• Title/Summary/Keyword: Extraction methods

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Extraction and Analysis fo CArthamin Contained in the Safflower (한국산 잇꽃 꽃잎의 유용성분에 관한 연구)

  • 박종선
    • Korean Journal of Plant Resources
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    • v.11 no.1
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    • pp.60-63
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    • 1998
  • For the purposed of improving the utilization of natural chemical pigment, carthamin, of Carthamus tinctorius, the effective extraction methods on this compound were pursued in the present study. The best solvent for the extraction was found to be the 1 %(v/v) NaOH solution, at 25 hours. In addition, more carthamin was extracted flowers from main stem than fromb-ranches . The carthamin content of Korean local safflower was shown to be higher than that of Japanese variety used for medicinal uses.

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Effects of feather processing methods on quantity of extracted corticosterone in broiler chickens

  • Ataallahi, Mohammad;Nejad, Jalil Ghassemi;Song, Jun-Ik;Kim, Jin-Soo;Park, Kyu-Hyun
    • Journal of Animal Science and Technology
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    • v.62 no.6
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    • pp.884-892
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    • 2020
  • Corticosterone is known as a biological stress index in many species including birds. Feather corticosterone concentration (FCC) has increasingly been used as a measure for chronic stress status in broiler chickens. As sample preparation is the first step of analytical process, different techniques of feather matrix disruption need to be validated for obtaining better result in analysing corticosterone extraction. The current study was a validation of pulverizing the feather by bead beater (BB) and surgical scissors (SS) processing prior to corticosterone extraction in feather of broiler chickens. The type of feather processing prior to the hormone extraction may alter the final output. Thereby, finding a standard method according to laboratory facilities is pivotal. This study carried out to determine the effects of feather pulverization methods on the extraction amount of corticosterone in broiler chickens. Feathers were sampled from four weeks old Ross 308 broiler chickens (n = 12 birds). All broiler chickens were kept under the same environmental condition and had access to feed and water. Feather samples were assigned to one of the following processing methods 1) using a BB for pulverizing and 2) using a SS for chopping into tiny pieces. Each sample was duplicated into two wells during enzyme immunoassay (EIA) analysis to improve the accuracy of the obtained data. The results showed lower standard errors and constant output of FCC by using the BB method compared with the SS method. Overall comparison of FCC showed a significantly higher (p < 0.001) amount of the FCC in the BB compared with the SS. Overall, using the BB method is recommended over the SS method for feather processing due to the ability to homogenize a large number of samples simultaneously, ease of use and greater extraction of feather corticosterone.

Separation and purification of elements from alkaline and carbonate nuclear waste solutions

  • Alexander V. Boyarintsev ;Sergei I. Stepanov ;Galina V. Kostikova ;Valeriy I. Zhilov;Alfiya M. Safiulina ;Aslan Yu Tsivadze
    • Nuclear Engineering and Technology
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    • v.55 no.2
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    • pp.391-407
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    • 2023
  • This article provides a survey of wet (aqueous) methods for recovery, separation, and purification of uranium from fission products in carbonate solutions during the reprocessing of spent nuclear fuel and methods for removal of radionuclides from alkaline radioactive waste. The main methods such as selective direct precipitation, ion exchange, and solvent extraction are considered. These methods were compared and evaluated for reprocessing of spent nuclear fuel in carbonate media according to novel alternative non-acidic methods and for treatment processes of alkaline radioactive waste.

초임계 이산화탄소를 이용한 유자 과피로부터 향기성분의 추출

  • Lee, Seung-Jin;Jeon, Byeong-Su
    • 한국생물공학회:학술대회논문집
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    • 2001.11a
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    • pp.701-704
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    • 2001
  • The volatile components of citron peel extracted by three methods, which were SDE (Simultaneous steam distillation & solvent extraction) extraction, solvent extraction and supercritical carbon dioxide extraction were analyzed with gas chromatography. The operation conditions was at the temperature between $30^{\circ}C$ and $45^{\circ}C$ and perssure between 1000 psi and 2500 psi. The aromatic compound was the principal component extracted, the optimum conditions for limonene extraction were 2000 psi and $40^{\circ}C$.

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Effects of Inhibition on Formation and Growth of Polymer in Butadiene Extraction Unit (Butadiene Extraction Unit 내의 Polymer 생성 억제 효과)

  • Im, Gyeong
    • The Journal of Natural Sciences
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    • v.5 no.2
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    • pp.63-73
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    • 1992
  • There are many methods of obtaining butadiene described in the literature. In the america it is produced largely from petroleum gases, i.e., by catalytic dehydrogenation of butene of butene-butane mixtures. Butadiene can be recovered from the $C_4$ residue of an olefin plant by distilling off a fraction containing most of the butadiene, catalytically hydrogenating the higher acetylenes to olefins and separating the product from other olefins and isobutane by extraction. Also it can be obtained by cracking naphtha and light oil. Among the individual dienes of commercial importance, 1, 3-butadiene is of first importance. It is used primarily for the production of polymers.In the present paper, it was investigated for a effect of the formation and the growth inhibition of popped corn polymer in butadiene extraction unit. As a result of study, inhibitors, $NaNO_2$ and TBC were good effective for inhibition of the formation and growth in popcorn polymer. The rational formula of popcorn polymer obtained was $(C_4H_6)_x$.

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Stroke Extraction of Chinese Character using Mechanism of Optical Neural Field (시각신경 메커니즘을 이용한 한자 획의 분리 및 추출)

  • Son, Jin-U;Lee, Uk-Jae;Lee, Haeng-Se
    • The Transactions of the Korea Information Processing Society
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    • v.1 no.3
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    • pp.311-318
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    • 1994
  • In this paper, a new stroke extraction method of Chinese character base on the human optical field(the Receptive Field of Cell) is proposed. In processing the feature extraction of the chinese character, needed are more perfect extraction methods for separated informations and its data base. This method can be applied to processing neural cell using conventional feature extraction mechanism in the optical boundary of retina and cerebrum. With this method, its applicability and effectiveness were demonstrated extracting strokes from Chinese character.

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Integration of Headspace Solid Phase Micro-Extraction with Gas Chromatography for Quantitative Analysis of Formaldehyde

  • Lo, Kong Mun;Yung, Yen Li
    • Bulletin of the Korean Chemical Society
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    • v.34 no.1
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    • pp.139-142
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    • 2013
  • A study was carried out to evaluate the solid phase micro-extraction (SPME) for formaldehyde emission analysis of uncoated plywood. In SPME, formaldehyde was on-fiber derivatized through headspace extraction and analyzed by gas chromatography coupled with mass spectrometry (GC/MS). The SPME was compared with desiccators (DC-JAS 233), small-scale chamber (SSC-ASTM D6007) and liquid-liquid extraction (LLE-EPA 556) methods which were performed in accordance with their respective standards. Compared to SSC (RSD 4.3%) and LLE (RSD 5.0%), the SPME method showed better repeatability (RSD 1.8%) and not much difference from DC (RSD 1.4%). The SPME has proven to be highly precise (at 95% confidence level) with better recovery (REC 102%). Validation of the SPME method for formaldehyde quantitative analysis was evidenced. In addition, the SPME by air sampling directly from plywood specimens (SPME-W) correlated best with DC ($r^2$ = 0.983), followed by LLE ($r^2$ = 0.950) and SSC ($r^2$ = 0.935).

Study of Nonlinear Feature Extraction for Faults Diagnosis of Rotating Machinery (회전기계의 결함진단을 위한 비선형 특징 추출 방법의 연구)

  • Widodo, Achmad;Yang, Bo-Suk
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2005.11a
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    • pp.127-130
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    • 2005
  • There are many methods in feature extraction have been developed. Recently, principal components analysis (PCA) and independent components analysis (ICA) is introduced for doing feature extraction. PCA and ICA linearly transform the original input into new uncorrelated and independent features space respectively In this paper, the feasibility of using nonlinear feature extraction will be studied. This method will employ the PCA and ICA procedure and adopt the kernel trick to nonlinearly map the data into a feature space. The goal of this study is to seek effectively useful feature for faults classification.

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The Simultaneous Analysis of Benzene, Toluene, Ethylbenzene, o,m,p-Xylenes and Total Petroleum Hydrocarbons in Soil by GC-FID after Ultra-Sonication

  • Sin, Ho Sang;Gwon, O Seung
    • Bulletin of the Korean Chemical Society
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    • v.21 no.11
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    • pp.1101-1105
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    • 2000
  • A simultaneous determination method of BTEX (benzene, toluene, ethylbenzene, o,m.p-xylene) and TPH (kerosene, diesel, jet fuel and bunker C) in soil with gas chromatography/flame ionization detection (GC-FID) was described. The effects of extracti on method, extraction solvent, solvent volume and extraction time on the extraction performance were studied. A sonication method was simpler and more efficient than Soxhlet or shaking methods. Sonication with 10 mL of acetone/methylene chloride (1 : 1, v/v) for 10 min was found to be optimal extraction conditions for 20 g of soil. Peak shapes and quantification of BTEX and TPH were excellent, with linear calibration curves over a wide range of 1-500 mg/L for BTEX and 10-5000 mg/L for TPH. Good reproducibilities by sonication were obtained, with the RSD values below 10%. By using about 20 g of soil, detection limits were 0.8 mg/L for BTEX and 10 mg/L for TPH. The advantages of this procedure are the use of simple and common equipment, reduced volumes of organic solvents, rapid extraction periods of less than 20 min, and simultaneous analysis of volatile and semivolatile compounds.

Isolation of High-Quality mRNA from Tannin-Rich Persimmon Fruit (고 Tannin 함유 감과실로 부터 mRNA의 분리)

  • ;Dav
    • Food Science and Preservation
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    • v.4 no.1
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    • pp.45-51
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    • 1997
  • In our studies on the role of $\beta$-galactosidase in fruit softening, significant difficulty, was encountered in our attempts to extract RNA from persimmon(Diospyros kaki L. cv. Fuyu) fruit due to astringency and tannin content. Initial, unsuccessful RNA extractions involved methods using guanidinium isothiocyanate/CsCl with and without polyvinylpyrrolidone(PVP), phenol/sodium lauryl sulfate(SDS), guanidinium hydrochloride, as well as polysomal RNA purification method that used 0.2 M Tris-HCI (pH 9.0) containing KCI, Mg-acetate, EDTA, $\beta$-mercaptoethanol, and sucrose. A method was devised which employed treatment of fruit with CO2 gas to diminish astringency prior to RNA extraction, followed by extraction of tissue powders with Proteinase K extraction buffer containing PVP and ascorbate at an alkaline pH. This procedure resulted in the removal of tannins and other polyphenolics and extraction of relatively large amount of high-quality RNA suitable for cDNA library construction and polymerase chain reaction(PCR). Futhermore, the procedure does not use the toxic and corrosive chemical guanidinium isothiocyanate or require ultracentrifugation.

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