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Induction on in vitro Plant Regeneration the Apple Rootstocks of Fire Blight Resistance by Plant Growth Regulators (생장조절제 처리에 따른 과수화상벙 저항성 사과대목의 기내 식물체 유도)

  • Young Hee Kwon;Won IL Choi;Hee Kyu Kim;Kyung Ok Kim;Ju Hyoung Kim;Yong Sup Song
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2021.04a
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    • pp.23-23
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    • 2021
  • Apple (Malus×domestica Borkh.; Rosaceae) is an important fruit crop grown mainly in temperate regions of the world. Tissue culture in vitro is a biotechnological technique that has been used to genetically improve cultivars (scions) and rootstocks. This could be important in the production of genetically uniform scions and rootstocks for commercial apple production. In nurseries, apple plants are produced by grafting scions onto rootstocks. The Cornell-Geneva (Geneva® series) breeding program has bred several dwarf rootstocks that are resistant to diseases and pests and are also cold hardy. This study was conducted to determine the optimal medium strength to improve sprouting shoot rate of apical meristem of the apple rootstocks of fire blight resistance. The apple rootstocks apical meristem at size (0.2 mm to 0.3 mm) with axillary buds were cultured on the MS(Murashige & Skoog) medium supplemented with plant growth regulators. The sprouting ratio and growth characteristics was evaluated after eight weeks in vitro culture. The highest rate of bud differentiation and shoot formation were 23.8% and 55.6%, respectively. After 6 weeks, shoots were regenerated from apical meristem, and their growth characteristics was significantly varied on the respective basal medium with different plant growth regulators. Our studies showed that the apple rootstocks the apple rootstocks of fire blight resistance plantlets could be successfully produced from apical meristem differentiated out of young twigs via organogenic regeneration.

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Plant Regeneration through Leaf Explant Culture of Boxthorn (Lycium chinense Mill.)

  • Ham, In-Ki;Park, Sang-Kyu;Lee, Bong-Chun;Lee, Mi-Ae;Kwon, Kyeong-Hak;Lee, Eun-Mo;Jo, Man-Hyun
    • Korean Journal of Plant Resources
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    • v.20 no.3
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    • pp.251-254
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    • 2007
  • This study was conducted to investigate the optimal plant growth regulator level for the shoot formation of Lycium chinense Mill. In vitro plant propagation was developed for leaf explants of boxthorn. Leaf explants were cultured on MS medium supplemented with cytokinins (BA and 2-iP) alone. Plants were successfully regenerated through in vitro culture by using leaf explants of boxthorn grown in the field. After 4 weeks of culture, 58% of shoot formation had developed from the leaf explants. The shoot formation rate of 'Jangmyeong' was highest followed by 'Myeongan', ;Cheondae', and 'Bullo'. The use of 0.2mg/L BA was critical for enhanced production of shoot formation and resulted in 58% of the culture producing shoot formations. Regenerated plantlets transplanted to pots were developed and successfully acclimatized to greenhouse.

Analysis of the Factors Involved in the Occurrence of Rice Stripe virus in Chungcheongbukdo in 2008 and 2009 (2008-2009년 충북지역 벼 줄무늬잎마름병 발생 요인 분석)

  • Kang, Hyo-Jung;Ahn, Ki-Su;Han, Chong-U;Jeong, Kyeong-Heon;Park, See-Jung;Ji, Jae-Jun;Kim, Jeong-Soo
    • Research in Plant Disease
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    • v.16 no.2
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    • pp.109-114
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    • 2010
  • The occurrence factors of Rice stripe virus (RSV) in Chungcheongbukdo were analyzed by investigating the viruliferous insect rate (VIR) of overwintered small brown plant hopper (SBPH), the population density of SBPH, the infection rate of natural host plants, and the occurrence rate of RSV on rice paddy fields at the 3 areas of Cheongwon, Jincheon, and Boeun in 2008 and 2009. The average VIR of overwintered SBPH was 0.0% in 2008 and 1.1% in 2009. From SBPH collected on early June in 2009, VIR was higher as 1.4% at Jincheon and 4.2% at Boeun than those of overwintered SBPH, and this higher VIR might relate stronlgy with the adult population of SBPH immigrated from China. The populations of SBPH at Cheongwon, Jincheon and Boeun in 2008 were 3.8, 7.5 and 20.8 Head/$m^2$, respectively. However, those of Cheongwon and Jincheon increased up to about two folds as 8.4 and 13.1 in 2009. No RSV was detected on the natural host plants including barley. The factors involved in RSV occurrence were affected negatively by the low VIR of overwintered SBPH, the low population of overwintered SBPH, the low infection rate of RSV on the natural host plants, and the clean cultivation environment in Chungcheongbukdo.

Effects of PAA (Polyaspartic Acid) Contained Complex Fertilizer on Rice Growth and CH4 emission from Rice Cultivation (PAA(Polyaspartic Acid) 함유 복합비료가 벼 생육 및 벼 재배 논에서의 메탄 발생에 미치는 영향 연구)

  • Ju, Okjung;Lee, Jeong-Hyung;Choi, Byoung-Rourl;Won, Tae-Jin;Cho, Kwang-Rae;Seo, Jae-Sun;Kim, Young-Sun;Park, In-Tae
    • Journal of Environmental Impact Assessment
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    • v.22 no.6
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    • pp.705-711
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    • 2013
  • This study was carried out to investigate the effects of the complex fertilizers containing polyaspartic acid (PAA) on growth and $CH_4$ emission in rice field and optimum application rate of the fertilizer compared to the standard recommended application rate (control). The PAA-containing complex fertilizers (PCF) were applied at 55, 65 and 75% levels of standard recommended application rate (control). The application rate of PAA in the plot of every PCF treatment was 150g ai/10a. The PCF was applied as a basal dressing without topdressing at tillering stage. The growth parameters of rice and its nitrogen use efficiency treated with PCF at a 65 to 75% level were not different compared with those of control, and the rice yield was also not significantly different between PCF at a 65 to 75% level and control during 2 years(2010~2011) field experiment. And the $NH_4$-N content in soil was not affected by 65% to 75% level of PCF treatment. Considering overall research results such as rice yield and growth parameters PCF is not significantly different with the control and the optimum application rate of the PCF as a basal fertilization was determined to be 65~75% of the standard application rate based on the result in rice cultivation. Moreover, $CH_4$ emission rate was significantly reduced by PCF treatments, showing 216 kg and 229 kg $CH_4/ha$ at 65% and 75% PCF treatment level, respectively, compared to 266 kg $CH_4/ha$ of the control.

Effect of thoracic cage mobilization on respiratory function, spinal curve and spinal movement in patients with restrictive lung disease

  • Wang, Joong San
    • Journal of International Academy of Physical Therapy Research
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    • v.7 no.2
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    • pp.1006-1010
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    • 2016
  • This study aimed to examine the effects of thoracic cage mobilization on the respiratory function, spinal curve and spinal movement in patients with restrictive lung diseases. The subjects were ten community-dwelling elderly with a restrictive lung diseases when measured using a spirometer($FEV1/FVC{\leq}65%$, FVC<80%). They received an intervention over an eight-week period: three times a week and for 30 minutes a day. SPSS for Windows(ver. 19.0) was used to analyze all the collected data. Independent t-tests were used to examine changes before and after the intervention. The study's results showed statistically significant improvement(p<.05) in forced expiratory volume in 1 second(chage rate: .$24{\pm}.25$), thoracic curve(chage rate: $-2.50{\pm}2.76$), lumbar curve(chage rate: $-.80{\pm}1.32$), thoracic flexion(chage rate: $2.10{\pm}1.52$), thoracic extension(chage rate: $-2.00{\pm}1.25$), lumbar flexion(chage rate: $2.40{\pm}3.13$) and lumbar extension(chage rate: $-1.30{\pm}1.42$). The results of this study suggest that the thoracic cage mobilization contribute to improve pulmonary function in patients with restrictive lung disease.

Incidence of Viral Diseases on Red Pepper in Yeongyang-Gun, Gyeongbuk Provice (경북 영양지역 노지고추의 바이러스병 발생양상)

  • Kwon, Oh-Hun;Lee, Joong-Hwan;Jang, Kil-Su;Kim, Dong-Jin;Kim, Chan-Yong;Jeon, Su-Gyeong;Kwon, Jung-Bae;Jung, Hee-Young
    • Research in Plant Disease
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    • v.23 no.3
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    • pp.234-240
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    • 2017
  • Incidence of virus diseases in red pepper of open field in Yeongyang-Gun, Gyeongbuk Province was investigated by reverse transcription-polymerase chain reaction in 2012-2016. The infection rate of Cucumber mosaic virus (CMV), Broad bean wilt virus 2 (BBWV2), Pepper mottle virus, Potato virus Y, Pepper mild mottle virus and Tomato spotted wilt virus was 46.1%, 41.5%, 2.0%, 2.0%, 4.4% and 0.1% respectively. Incidence rate of single and mixed infection was 31.2% and 62.6%. Most of single infections were CMV and BBWV2. Among mixed infections, the incidence rate of CMV+BBWV2 mixed infection was the highest as 49.3% and most of mixed infections of triplex and tetraplex included CMV+BBWV2 mixed infection. CMV single infection caused mosaic, chlorosis, yellowing and vein necrosis and BBWV2 single infection induced cholosis and mosaic. CMV+BBWV2 mixed infection caused severe mosaic with chlorosis or malformation.

Effect of Drying Temperature on High Quality Functional Processed Products of Chinese Matrimony Vine (가공용 소재로서 구기자나무 잎의 건조온도 조건)

  • Ju, Jung Il;Lee, Jeong;Paik, Seung Woo;Yun, Tug Sang;Park, Young Chun;Lee, Bo Hee;Kim, Hyun Ho;Lee, Hee Bong
    • Korean Journal of Medicinal Crop Science
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    • v.23 no.6
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    • pp.468-472
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    • 2015
  • Background : Chinese matrimony vine (Lycium chinense Mill.) is a deciduous shrub belonging to the Solanaceae. The leaves are used as an alternative raw material replacing dried fruits associated with high production costs in many industires. The aim of this experiment was to determine the effect of drying temperature on the leaves used in the manufacture of functional products. Methods and Results : The leaves of Chinese matrimony vine were harvested when the plant height reached 60 - 70 cm in the spring and treated at four different temperatures ($40^{\circ}C$, $50^{\circ}C$, $60^{\circ}C$ and $70^{\circ}C$). The time to reach the desiccation state of 20% was 63 h at $40^{\circ}C$, 37 h at $50^{\circ}C$, 17 h at $60^{\circ}C$ and 11 h at $70^{\circ}C$. The drying rate per hour was 1.28% at $40^{\circ}C$, 2.25% at $50^{\circ}C$, 4.94% at $60^{\circ}C$ and 7.60% at $70^{\circ}C$. No significant difference were observed in ash, crude fat, polyphenol or rutin content of treated samples. Crude protein and betaine content decreased with higher dry temperatures. Nitrogen free extract of the treated samples increased with higher drying temperatures. Conclusions : Taking into consideration drying time, drying rate, color value, energy consumption and functional ingredients is advantageous to dry the leaves of the Chinese matrimony vine at $60^{\circ}C$ using a hot air agricultural dryer.

Analyzing the Effect of Climatic Variables on Growth and Yield of Rice in Chuncheon Region (춘천지역의 기상요소가 벼 생육과 수량에 미치는 영향 해석)

  • Lee, An-Soo;Kim, Jae-Rok;Cho, Youn-Sang;Kim, Yong-Bog;Ham, Jin-Kwan;Jeong, Jeong-Su;Sa, Jong-Gu;Shin, Jin-Chul
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.56 no.2
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    • pp.99-106
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    • 2011
  • Here we reported an analyzing result for the relationship between climatic variables and rice(c.v. Odaebyeo and Ilpumbyeo) yield characteristics (including some growth characteristics) based on a long-term observed data at GARES and at KMA for rice and weather, respectively. Most of crop parameters investigated, such as heading date, culm height, panicle number $m^{-2}$, grain number $panicle^{-1}$ ripening rate, 1,000 grain weight and yield were strongly affected by wind velocity and relative humidity, as well as by daily mean air temperature, precipitation, sunshine hours and daily variations in air temperature depending on variety and crop developmental stages. Air velocity and relative humidity had not been studied as climatic variables affecting on the characteristics of rice growth and yield, however, they turned out to affect all the characteristics of rice investigated, especially ripening rate and yield, as much as any other climatic variables in this study. Air velocity appeared to affect highly the culm height and yield of Odaebyeo and ilpumbyeo. Relative humidity appeared to affect highly grain number and ripening rate of Odaebyeo and yield of Ilpumbyeo. Consequently Rice yield revealed to increase in the climatic conditions of high air velocity and low relative humidity.

Occurrence Status of Five Apple Virus and Viroid in Korea (국내 주요지역의 사과 바이러스 및 바이로이드 5종의 발생 현황)

  • Lee, Seongkyun;Cha, Jae-Soon;Kwon, Yeuseok;Lee, Yun Sang;Yoo, Se Eun;Kim, Ju Hyung;Kim, Daeil
    • Research in Plant Disease
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    • v.26 no.2
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    • pp.95-102
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    • 2020
  • The investigation of the infection rate of domestic apple orchards by four types of apple viruses (Apple chlorotic leaf spot virus [ACLSV], Apple stem pitting virus [ASPV], Apple stem grooving virus [ASGV], Apple mosaic virus [ApMV]) and one type of viroid (Apple scar skin viroid, ASSVd) found that most apple trees were infected with viruses and viroid at the rate of 97.3%. By region, the infection rate in Jeongseon stood at 98.8%, Danyang at 100%, Yesan at 100%, Jangsu at 89.1%, and Muju at 98.1%. By each virus and viroid, the infection rate of ASGV was the highest at 93.4%, followed by ASPV at 85.7%, ACLSV at 59.0%, ASSVd at 6.7%, and ApMV at the lowest 3.6%. In addition, 84.8% of the cases were infected with two or more types of viruses and viroid, nearly seven times the single type infection rate of 12.4%, and the cases infected with three viruses, ASPV, ACLSV, and ASGV accounted for 56.2%, more than the half the total number of trees investigated.

Effect of the Slow-releasing Fertilizer Applied Directly to Rice Seedling Tray Before Transplanting and Practical Test at Field (이앙직전 벼 육묘상자 살포용 완효성 비료의 처리 효과)

  • Chi, Jeong-Hyun;Choi, Byoung-Rourl;Jo, Gwang-Lae;Kim, Soon-Jae;Park, Kyeong-Yeol;Kwon, O-Youn
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.58 no.1
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    • pp.8-14
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    • 2013
  • The newly developed fertilizer is the slow-releasing fertilizer which can be used as a basal fertilizer without no additional fertilization at tillering stage. It has 30-4-6% of $N-P_2O_5-K_2O$ and was coated with mixture of LDPE (Low density polyethylene), EVA (Ethylene vinyl acetate), BDP (Bio degraded polymer), TALC and nonionic surfactant for the controlled release up to 50 days after application. Coating materials were designed to be decomposed naturally. This fertilizer can be applied directly to the seedling tray mechanically just before transplanting, resulting in significant labor saving effect. The developed slow-release fertilizer, which can replace both basal fertilization and top dressing at tillering stage by single application directly to seedling tray, showed the highest release at 14~21 days after transplanting. Considering the plant growth at different growth stages and yield, the optimal application rate of developed slow-release fertilizer was 300 g per rice nursery tray and the yield of rice at this application rate was 5.25 MT/ha. Rice quality in terms of head rice grain ratio, amylose content, whiteness, and taste value decreased as fertilization rate increased from 200 g to 500 g per nursery tray. Fertilization rate based on quantity of fertilizer ingredients (N, P, K) was reduced by 49.3% compared to the standard application rate and there was 49.2% reduction in labor input for fertilization.