• Korean Journal of Microbiology
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• v.43 no.3
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• pp.166-172
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• 2007

ERM proteins transfer the methyl group to $A_{2058}$ in 23S rRNA, which reduces the affinity of MLS (macrolide-lincosamide-streptogramin B) antibiotics to 23S rRNA, thereby confer the antibiotic resistance on micro-organisms ranging from antibiotic producers to pathogens and are classified into monomethyltransferase and dimethyltransferase. To investigate the differences between mono- and dimethyltransferase, tirD, a representative monomethylase gene was cloned in Escherichia coli from Streptomyces fradiae which contains ermSF, dimethylase gene as well to overexpress the TlrD for the first time. T7 promoter driven expression system successfully overexpress tlrD as a insoluble aggregate at $37^{\circ}C$ accumulating to around 55% of the total cell protein but unlike ErmSF, culturing at temperature as low as $18^{\circ}C$ did not make insoluble aggregate of protein into soluble protein. Coexpression of Thioredoxin and GroESL, chaperone was not helpful in turning into soluble protein either as in case of ErmSF. These results might suggest that differences between mono- and dimethylase could be investigated on the basis of the characteristics of protein structure. However, a very small amount of soluble protein which could not be detected by SDS-PAGE conferred antibiotic resistance on E. coli as in ErmSF which was expected from the activity exerted by monmethylase in a cell.

• The Journal of the Korea Contents Association
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• v.17 no.1
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• pp.634-661
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• 2017

This study examines the procedure of successful novel adaptation. It is well known from precedent studies that narrative structure of novels should change forms to suit the media characteristics of films. But, the changing forms of narrative structure is not a sufficient condition of successful novel adaptation, but a necessary condition. A successful adaptation could be completed with filmic expressions on the presented narrative structure. The core of filmic expression is cinematic language which means the composition and array of image and sound. The novel, Kirishma Thing deals with everyday life of high school students and it consists of six stories which are narrated by one student each in first person point of view. The film, Kirishma Thing implemented a different strategy. It reveals the same events several times to show many characters over in each person's point of view in the first half. In the second half, all the characters gathers at the rooftop of the school to have an unilinear narrative structure over one event. This film utilize all kinds of cinematic language to achieve these structures including the widescreen aspect ratio which exposes as many characters as possible in one shot, picture composition which shows the same event in a different point of view, contrast in lighting and music which differentiates and empowers the last sequence of the film.

• Journal of Science Education
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• v.34 no.1
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• pp.33-46
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• 2010

The purpose of this study was to find out the image as science teachers recognized by pre-service science teachers. The data was collected from 312 pre-service science teachers from Kyungpook National University in Daegu and participants were asked to write about the image of science teachers they liked most and least in their secondary school years freely. The result of this research was as follows. The image as science teachers categorized 2 factors: science instructional situation, image of science teacher. Each factor was subdivided into more detailed ones. First of all, 'science instructional situation' category subdivided into lesson style, teaching-learning materials, teaching methods, and class atmosphere. In lesson style, 'experiment' and 'observation' gained the most favorable comments, and questioning-answering gained the least. In teaching-learning materials, print materials such as handouts, worksheets, reports were the most liked, and 'writing on the blackboard' was the least liked. In teaching methods, the 'detailed and systematic explanation of the theory and concepts' was preferred to rote learning and memorization lacking explanation. In class atmosphere, friendly and free atmosphere was the most preferred, and uncomfortable, boring one was the least preferred. Secondly, in 'image of the science teachers' category and 'quality as the teachers' sub-category, thoughtful and considerate teachers who respect students' personality was the most preferred. On the contrary, they didn't prefer teachers who were indifferent and humiliated students. Finally in 'characteristics of the teachers' sub-category, the participants liked clear, energetic voice, and mild expression, and they didn't like formal style, overly fancy clothes, etc. Based on the result of this study, more empirical study on the teachers' image is needed, and the thoughts of educational administrators, students, parents, and teachers should be reflected because an undesirable teacher can be advised and get opportunity to be a better teacher.

• Smart Media Journal
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• v.7 no.1
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• pp.37-44
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• 2018

Utilizing sequence control and numerical computing, embedded devices are used in a variety of automated systems, including those at industrial sites, in accordance with their control program. Since embedded devices are used as a control system in corporate industrial complexes, nuclear power plants and public transport infrastructure nowadays, deliberate attacks on them can cause significant economic and social damages. Most attacks aimed at embedded devices are data-coded, code-modulated, and control-programmed. The control programs for industry-automated embedded devices are designed to represent circuit structures, unlike common programming languages, and most industrial automation control programs are designed with a graphical language, LAD, which is difficult to process static analysis. Because of these characteristics, the vulnerability analysis and security related studies for industry automation control programs have only progressed up to the formal verification, real-time monitoring levels. Furthermore, the static analysis of industrial automation control programs, which can detect vulnerabilities in advance and prepare for attacks, stays poorly researched. Therefore, this study suggests a method to present a discussion on an industry automation control program designed to represent the circuit structure to increase the efficiency of static analysis of embedded industrial automation programs. It also proposes a medium term translation technology exploiting LLVM IR to comprehensively analyze the industrial automation control programs of various manufacturers. By using LLVM IR, it is possible to perform integrated analysis on dynamic analysis. In this study, a prototype program that converts to a logical expression type of medium language was developed with regards to the S company's control program in order to verify our method.

• Proceedings of the Korean Society for Bioinformatics Conference
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• 2006.02a
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• pp.83-89
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• 2006

Cellular functions are carried out by a concerted action of biochemical pathways whose components have genetic interactions. Abnormalities in the activity of the genes that constitute or modulate these pathways frequently have oncogenic implications. Therefore, identifying the upstream regulatory genes for major biochemical pathways and defining their roles in carcinogenesis can have important consequences in establishing an effective target-oriented antitumor strategy We have analyzed the gene expression profiles of human liver cancer samples using cDNA microarray chips enriched in liver and/or stomach-expressed cDNA elements, and identified groups of genes that can tell tumors from non-tumors or normal liver, or classify tumors according to clinical parameters such as tumor grade, age, and inflammation grade. We also set up a high-throughput cell-based assay system (cell chip) that can monitor the activity of major biochemical pathways through a reporter assay. Then, we applied the cell chip platform for the analysis of the HCC-associated genes discovered from transcriptome profiling, and found a number of cancer marker genes having a potential of modulating the activity of cancer-related biochemical pathways such as E2F, TCF, p53, Stat, Smad, AP-1, c-Myc, HIF and NF-kB. Some of these marker genes were previously blown to modulate these pathways, while most of the others not. Upon a fast-track phenotype analysis, a subset of the genes showed increased colony forming abilities in soft agar and altered cell morphology or adherence characteristics in the presence of purified matrix proteins. We are currently analyzing these selected marker genes in more detail for their effects on various biological Processes and for Possible clinical roles in liver cancer development.

• Journal of the Korean Institute of Intelligent Systems
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• v.22 no.2
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• pp.192-197
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• 2012

With the advent of DNA microarray technologies, researches for disease diagnosis has been actively in progress. In typical experiments using microarray data, problems such as the large number of genes and the relatively small number of samples, the inherent measurement noise and the heterogeneity across different samples are the cause of the performance decrease. To overcome these problems, a new method using functional modules (e.g. signaling pathways) used as markers was proposed. They use the method using an activity of pathway summarizing values of a member gene's expression values. It showed better classification performance than the existing methods based on individual genes. The activity calculation, however, used in the method has some drawbacks such as a correlation between individual genes and each phenotype is ignored and characteristics of individual genes are removed. In this paper, we propose a method based on the ensemble classifier. It makes weak classifiers based on feature vectors using subsets of genes in selected pathways, and then infers the final classification result by combining the results of each weak classifier. In this process, we improved the performance by minimize the search space through a filtering process using gene-gene interaction information and the mRMR filter. We applied the proposed method to a classifying the lung cancer, it showed competitive classification performance compared to existing methods.

• Journal of Korean Society of Forest Science
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• v.99 no.3
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• pp.298-303
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• 2010

To explore on the defense strategies against hervivory of Juglans regia and J. mandshurica, morphological characteristics of the leaf, leaf domatia structure and the number, herbivores insects and mites on the leaves, collected from the trees growing in Mt. Chiak, Mt. Cheongtae, Mt. Jungwang, Namyangju-si and Wonju-si, were investigated from May to October, 2009. Domatia of J. mandshurica revealed tuft type, these of J. regia revealed pocket+tuft type. Domatia number per leaflet proved the higher figures, 28.3/leaflet for J. mandshurica, and the lower, 19.6/leaflet for J. regia. Leaf surface trichomes of J. regia revealed stellates only on the domatia structures, and that of J. mandshurica does dense stellates and glandular hairs on the leaf-blade and vein. Predatory mites' number per leaflet proved highly significant differences among tree species, and mean of predatory mites was higher values (4.8/leaflet) in J. mandshurica and lower values (3.5/leaflet) in J. regia. Small amount of nectar are found distal veinparts on the leaf margin of J. mandshurica. Dense glandular hairs on the lower leaf surfaces of J. mandshurica estimates useful strategy against herbivory. It may be possible to breed J. regia for better expression of leaf traits such as dense glandular hairs that increase predator populations and efficacy.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.04a
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• pp.59-59
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• 2018

Panax ginseng Meyer (Korean ginseng) is in the spotlight of Oriental medicine and is proclaimed as the king of medicinal plants owing to its adaptogenic characteristics. Ginseng root rot is a devastating disease caused by the fungus, Ilyonectria mors-panacis that generally attacks younger roots (~2 years), leading to defects in root quality, ginsenoside accumulation and also life cycle of the plant. Hence, there is an indispensable need to develop strategies resulting in tolerance against ginseng root rot. In the present study, we evaluated the effect of silica nanoparticles(N-SiO2) in Panax ginseng during I. mors-panacis infection. Long term analysis (30 dpi) revealed a striking 50% reduction in disease severity index upon 1mM and 2mM treatment of N-SiO2. However, N-SiO2 did not have any direct antifungal activity against I. mors-panacis. Membrane bound sugar efflux transporter, SWEET (Sugars Will Eventually be Exported Transporters) was identified in ginseng and as expected, its expression was suppressed upon N-SiO2 treatment in the root rot pathosystem. Furthermore, the total and reducing sugars in the apoplastic fluid clearly revealed that N-SiO2 regulates sugar efflux into apoplast. In a nut shell, N-SiO2 administration induces transcriptional reprogramming in ginseng roots, leading to regulated sugar efflux into apoplast resulting in enhanced tolerance against I. mors-panacis.

• Korean journal of applied entomology
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• v.55 no.4
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• pp.413-419
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• 2016

Ryanodine receptors (RyRs) regulate the contractions of insect muscles by altering intracellular $Ca^{2+}$ concentration and are the targets of chlorantraniliprole. Recently, a chlorantraniliprole-resistant strain was reported in the diamondback moth Plutella xylostella by obtaining point mutations on the RyRs. In the present study, we established two resistant strains from Drosophila melanogaster, which were treated with low or high concentrations of chlorantraniliprole, and their resistance levels were determined on the basis of contact and ingestion toxicities. Compared with the control strain, the two resistant strains did not show any significant differences in contact toxicity. However, they showed significantly increased resistance ratios in ingestion toxicity than that by the control strain. The low and high concentration resistant strains exhibited 2.1- and 8.1-fold increased resistance ratios, respectively, compared with that by the control strain. Moreover, we found that the resistant strains had altered expression levels of RyRs and more enhanced Acetylcholinesterase and Glutathione-S-transferase activities than that by the non-selected strain. These results suggested that the resistance development of chlorantraniliprole in the two strains might be mediated by the activation of detoxification pathways in D. melanogaster.

• Korean journal of communication and information
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• v.47
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• pp.120-147
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• 2009

The purpose of this paper is to promote bilateral friendly relations, the elements that hinder Hate Korea wave's(Kenkanryu) status and an analyzation of the characteristics of its causes and solutions for it. The Hate Korea wave is to be discussed in the fountain Hate Korea wave 2ch(2channel, ni channeru) acquaintances of information and representation to analyze the causes of this Japanese anti-Korean thinking. All of society should be aware of the differences and see what we have in common. I researched discussions of 2ch information and the content of other similar media. The expression was very violent. However, there was both general awareness of a gap between criticism and support. The gap was about what is not unusual on the Internet and not to be greatly concerned about. I also compares anti-Korean's awareness to Korea with general Japanese's, and finds that there are gap between them. For example by analyzing general Japanese's awareness we can find 18% of people who criticize the Hate Korea wave or have positive image of Korea. futhermore the proportion of anti-Korean contents in internet is not very high, so we don't have to worrying about the phenomenon seriously. but because of the following three points, there are sufficient needs to concern about the phenomenon constantly. First, sources of Hate Korea wave are traditional media. Second, the consumers of Hate Korea wave are mainly young generation who will make future relation of two countries. And last, Hate Korea wave include potential power to explode when certain conditions are met.