• Genomics & Informatics
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• 제16권4호
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• pp.19.1-19.11
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• 2018

MicroRNAs (miRNAs), small non-coding RNAs, have been implicated in various diseases and cellular functions as microregulators of gene expression. Although the history of miRNA investigation in autoimmune $Sj{\ddot{o}}gren^{\prime}s$ syndrome (SjS) is fairly short, a substantial amount of data has already been accumulated. These findings clearly indicate potential clinical implications of miRNAs, such as autoantigen expression and autoantibody production, viral miRNAs regulating the calcium signaling pathway, and aberrant immune cell regulation and cytokine production. Research endeavors in the field are currently underway to select disease-specific diagnostic and prognostic biomarkers by utilizing different types of tissues or biological specimens of SjS patients. Various techniques for miRNA analysis with different stringencies have been applied, with the most recent one being next-generation sequencing. This review compiles and highlights differentially-expressed miRNAs in various samples collected from SjS patients and their potential implications in the pathogenesis of SjS. To facilitate the development of miRNA-targeted personalized therapy in the future, we urge more follow-up studies that confirm these findings and elucidate the immunopathological roles of differentially-expressed miRNAs. Furthermore, improved diagnostic criteria for the disease itself will minimize sampling errors in patient recruitment, preventing the generation of inconsistent data.

• BMB Reports
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• 제40권2호
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• pp.226-231
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• 2007

Housekeeping genes are widely used as internal controls in a variety of study types, including real time RT-PCR, microarrays, Northern analysis and RNase protection assays. However, even commonly used housekeeping genes may vary in stability depending on the cell type or disease being studied. Thus, it is necessary to identify additional housekeeping-type genes that show sample-independent stability. Here, we used statistical analysis to examine a large human microarray database, seeking genes that were stably expressed in various tissues, disease states and cell lines. We further selected genes that were expressed at different levels, because reference and target genes should be present in similar copy numbers to achieve reliable quantitative results. Real time RT-PCR amplification of three newly identified reference genes, CGI-119, CTBP1 and GOLGAl, alongside three well-known housekeeping genes, B2M, GAPD, and TUBB, confirmed that the newly identified genes were more stably expressed in individual samples with similar ranges. These results collectively suggest that statistical analysis of microarray data can be used to identify new candidate housekeeping genes showing consistent expression across tissues and diseases. Our analysis identified three novel candidate housekeeping genes (CGI-119, GOLGA1, and CTBP1) that could prove useful for normalization across a variety of RNA-based techniques.

• 복식문화연구
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• 제19권3호
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• pp.518-530
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• 2011

Based on the development of digital technology, Photoshop as a computer graphics program has extended its range of application to creative techniques that can express the contemporary sense of fashion illustration. Thus, the objectives of this study are to seek a new technique of fashion illustration through a study of computer graphics programs required for vitalization in this digital era and to present the application. This is done after a literature review and empirical investigation. For the literature review, specialty publications with precedent studies related to the subject of this study are examined, and internet materials with related key words are sought to establish the reference data for the study. For the empirical investigation, cases of each expression for blending mode are examined to seek the correct means of fashion illustration. Five works are created as a result. The specific contents of this study are as follows: First, the types and characteristics of blending mode are examined. Second, cases in which the blending mode is applied are analyzed to seek insight into their utilization. Third, five works are created by applying the blending mode to present the result.

• 패션비즈니스
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• 제21권4호
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• pp.90-104
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• 2017

In a rapidly changing fashion design world, creative ideas are always required. Knowledge has been created as an art, exhibiting a new imagination that surpasses reality, while being visualized from the past. The purpose of this study is to derive types of visualization of knowledge for continuous creation of fashion design. The study consists of literature and empirical studies. ATTA, a creativity evaluation method made by Torrance applied for analyzing of Gucci fashion design collections from 2016-2017. Creativity of the Gucci collection by ATTA evaluation items are that first, a vivid idea was revealed through collection history, myths and animal and plants, second, conceptual incongruity is in the composition of the garment, third, provocative questions are the symbolism of the meaning of the text, fourth, different perspectives derive a new formative beauty through the synthesis of twisted elements, fifth, abstraction is a symbolic expression of animals and plants, sixth, synthesis is a mixture of various materials and techniques by plural inspiration, seventh, context, it is developed as a design accompanied by stories of history and myth and eighth, fantasy is fictitious animals and animals and detail. In addition, formativeness of fashion design by visualization of this knowledge was extracted with contamination, symbolism, enjoyment and fabrication. Visualization of the knowledge is expected to be used as a strategy to attract ongoing ideas for creative fashion designs.

• 만화애니메이션 연구
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• 통권10호
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• pp.149-157
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• 2006

인터넷이 생활매체로 자리잡으면서 본격적인 잔자상거래 시대가 도래함에 따라 온라인 시장의 성장 그래프는 가파르게 올라가고 있으며 인터넷 광고 시장의 규모도 빠르게 성장하고있다. 기존의 수동적 정보전달 형식과 달리 통신과 네트워크 기술의 발달로 능동적 쌍방향 커뮤니케이션이 가능한 온라인 매체는 광고 효과나 사용자의 반응을 즉각적으로 측정할 수 있는 장점을 가지고 있다. 리치미디어는 이러한 인터넷 에서의 신 기법 광고들을 지칭하며 향후 인터넷 광고시장을 주도할 것으로 전망 되고 있다. 지금까지의 인터넷 광고보다 상호작용성이 뛰어난 리치미디어 광고를 인지하고 리치 미디어 광고의 독특한 표현 요소들을 분석함으로써 리치미디어가 인터넷광고에 미치는 영향과 효과적인 활용방안을 연구하고자 한다.

• BMB Reports
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• 제45권12호
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• pp.677-685
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• 2012

In the process of tumorigenesis, normal cells are remodeled to cancer cells and protein expression patterns are changed to those of tumor cells. A newly formed tumor microenvironment elicits the immune system and, as a result, a humoral immune response takes place. Although the tumor antigens are undetectable in sera at the early stage of tumorigenesis, the nature of an antibody amplification response to antigens makes tumor-associated autoantibodies as promising early biomarkers in cancer diagnosis. Moreover, the recent development of proteomic techniques that make neo-epitopes of tumor-associated autoantigens discovered concomitantly has opened a new area of 'immuno-proteomics', which presents tumor-associated autoantibody signatures and confers information to redefine the process of tumorigenesis. In this article, the strategies recently used to identify and validate serum autoantibodies are outlined and tumor-associated antigens suggested until now as diagnostic/prognostic biomarkers in various tumor types are reviewed. Also, the meaning of autoantibody signatures and their clinical utility in personalized medicine are discussed.

• 한국실내디자인학회논문집
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• 제17권3호
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• pp.139-147
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• 2008

Modern architecture's optical mechanism focused on Ocuularcentrism neglects the tactility of vision and tends to eliminate the optical and tactile dualism of traditional spaces by representing spaces and surfaces that are abstract and cold-hearted. In other words, all sensory experiences, except for visual experiences, are eliminated to make it impossible to create the substantial core of architecture that combines time, image, and surface textures. The fast-changing social trends, the emergence of new materials and technologies, and the corresponding development of various types of media since the Industrial Revolution have changed the paradigm of human perception and representation. With the development of media, other sensory experiences besides visual experience have been stressed and human perception has converted from single perspective to complex perspective. In result, new sensory items, such as visual tactility, have replaced the traditional vision-centered hierarchy. The composition of architectural surfaces has represented the functional and commercial needs of technology, structure, as well as the socio-cultural needs of the community. In contemporary times, it is being changed and developed by the new tactility and the corresponding expression of modern architecture. Based on the visual representation of tactility of architectural surface, this study used a composition of surface that combines various events, meanings, and senses to examine how architecture can mediate and reproduce viewers' visual experiences and discover the existential relationship between architecture and men.

• International Journal of Fuzzy Logic and Intelligent Systems
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• 제12권3호
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• pp.250-255
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• 2012

Face recognition has wide applications in security and surveillance systems as well as in robot vision and machine interfaces. Conventional challenges in face recognition include pose, illumination, and expression, and face recognition at a distance involves additional challenges because long-distance images are often degraded due to poor focusing and motion blurring. This study investigates the effectiveness of applying photon-counting linear discriminant analysis (Pc-LDA) to face recognition in harsh environments. A related technique, Fisher linear discriminant analysis, has been found to be optimal, but it often suffers from the singularity problem because the number of available training images is generally much smaller than the number of pixels. Pc-LDA, on the other hand, realizes the Fisher criterion in high-dimensional space without any dimensionality reduction. Therefore, it provides more invariant solutions to image recognition under distortion and degradation. Two decision rules are employed: one is based on Euclidean distance; the other, on normalized correlation. In the experiments, the asymptotic equivalence of the photon-counting method to the Fisher method is verified with simulated data. Degraded facial images are employed to demonstrate the robustness of the photon-counting classifier in harsh environments. Four types of blurring point spread functions are applied to the test images in order to simulate long-distance acquisition. The results are compared with those of conventional Eigen face and Fisher face methods. The results indicate that Pc-LDA is better than conventional facial recognition techniques.

• 한국어교육
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• 제25권1호
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• pp.115-144
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• 2014

The purpose of this paper is to compare the two different instruction methods for Korean learners of academic purposes in learning request expression. Participants were divided into two groups, explicit and implicit group. Both groups viewed several scenes from the drama that involved native speakers interacting in different situations. The instructional treatment for the explicit group included metapragmatic information while the treatment for the implicit group did not. On the other hand, the treatment for the implicit group followed implicit techniques, which were repetition of the video presentation and a script reading activity. This study was made up of a pre-test, a post-test, and a delayed-test. The pre-test was conducted prior to the instructional treatment. The post-test was administered a day after the last instruction and the delayed-test was conducted five weeks after the treatments. Two types of tests, speaking and writing, were used in this study to examine subjects' knowledge of Korean request. The result of this research reveals that implicit treatment was more effective than explicit treatment in Korean learners' request acquisition. This results might have been due to the operationalization of the implicit condition in this study. Implicit instruction may help language learners make rules by themselves through tasks.

• 만화애니메이션 연구
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• 통권15호
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• pp.151-164
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• 2009

민화(民畵)에 대한 논의는 지금까지 여러 방향으로 지속되어왔다. 회화 작가들에 의해 응용되며, 또한 디자인의 기본 자료로 활용되기도 한다. 이는 대부분 '한국의 미'라는 관점에서 용인되어 시도되는 것들이다. 그만큼 민화는 한국의 대중적인 회화를 상징하고 잊혔던 정신의 한 면으로 통용되는 것이다. 그러나 많은 활용에도 불구하고 민화의 뚜렷한 독창성에 비해 명맥만 유지하는 현상이다. 만화의 특성과 많은 공통점이 있음에도 불구하고 과감한 만화의 시도는 보이지 않는다. 따라서 과장과 생략, 풍자와 시사성, 대중적인 그림화법, 희화적 요소, 이상향의 세계표현, 작가마다의 다른 이해에 의한 화법 등 민화의 특징에서 독창적인 그림을 분류하고 연구하여 만화적인 화법에 적극 활용할 수 있는 계기가 되고자 한다. 민화의 유형은 대부분 그림 내용으로 분류한다. 그러나 이 논문의 연구 목적이 민화 속에 만화적인 요소를 찾아내는 것이므로 동물, 식물, 인물, 곤충, 어류, 조류, 자연현상 등 그린 대상으로 크게 묶어 분석하였다. 이러한 분류는 대상의 캐릭터화에 대한 접근이 용이한 장점이 있으며 적극적인 접근을 유도하기 위한 것이다.