• Korean Journal of Microbiology
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• v.25 no.2
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• pp.103-109
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• 1987

An ozone-sensitive mutant of Escherichia coli strain B, MQ 1844 is described. Its properties, including high sensitivity to ozone and radiation, inducible filamentation, extensive DNA degradation and impaired DNA synthesis following ozonation, are attributable to a mutation in ozrB, a gene which is cotransducible with malB. Based on differences in phenotypic expression as well as on the particular location of this gene on the bacterial chromosome, ozrB appears as distinct from the other ozone-or radiation-sensitivity genes previously described.

• Proceedings of the Korea Contents Association Conference
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• 2018.05a
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• pp.319-320
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• 2018

여러 단계를 걸쳐 이루어지는 RNA-seq 분석 과정을 한 번에 처리할 수 있는 shell script 파이프라인을 구축하였다. 연구자들로 하여금 trimming, quality control, mapping, assembly, quantification 등 개별 과정을 거치지 않고, 한 줄의 커맨드 라인(command line) 만으로 유전자 발현량과 상대적 발현량 차이를 확인할 수 있는 fold change(FC) 값까지 얻을 수 있도록 하였다.

• Journal of Aquaculture
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• v.14 no.1
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• pp.9-16
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• 2001

Characterization of a single pass of cDNA sequence, an expressed sequence tag (EST) has been a fast growing activity in fish genomics. Despite its relatively short history, fish EST databases (dbESTs) have already begun to play a significant role in bridging the gaps in our knowledge on the gene expression in fish genome. This review provides a brief description of the technology for establishing fish dbESTs, its current status, and implication of the ESTs to aquaculture and fisheries science with particular emphasis on the discovery of novel genes for transgenic application, the use of polymorphic EST markers in genetic linkage mapping and the evaluation of signal-responsive gene expression.

• Journal of Mechanical Science and Technology
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• v.17 no.12
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• pp.1922-1927
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• 2003

Nonlinear normal mode (NNM) vibration, in a nonlinear dual mass Hamiltonian system, which has 6$\^$th/ order homogeneous polynomial as a nonlinear term, is studied in this paper. The existence, bifurcation, and the orbital stability of periodic motions are to be studied in the phase space. In order to find the analytic expression of the invariant curves in the Poincare Map, which is a mapping of a phase trajectory onto 2 dimensional surface in 4 dimensional phase space, Whittaker's Adelphic Integral, instead of the direct integration of the equations of motion or the Birkhoff-Gustavson (B-G) canonical transformation, is derived for small value of energy. It is revealed that the integral of motion by Adelphic Integral is essentially consistent with the one obtained from the B-G transformation method. The resulting expression of the invariant curves can be used for analyzing the behavior of NNM vibration in the Poincare Map.

• The Korean Journal of Food And Nutrition
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• v.6 no.4
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• pp.314-321
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• 1993

We have cloned the Bacillus cellulyticus K-12 avicelase (Avi, E.C.3.2.1.4) gene (ace A) In E. coli. This was accompanied by using the vector PT7T3U 19 and Hind W -Hind m libraries of Bacillus cellulyticus K-12 chromosomal inserts created in 5.cofi. The Libraries were screened for the expression of avicelase by monitoring the immunoreaction of the anti-avicelase (immunoscreening). Positive clones (Ac-3, Ac-5, and Ac-7) contained the identical 3.5kb Hind III fragment as determined by restriction mapping and Southern hybridization, and expressed avicelase efficiently and constituvely using its own promoter in the heterologous host. From the immunoblotting analysis, a polypeptide which showed a CMCase activity with an Mr of 54000 was detected.

• The Journal of Korean Academy of Prosthodontics
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• v.55 no.4
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• pp.361-371
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• 2017

Purpose: We aimed to investigate the gene expression of human gingival fibroblasts on microgroove surface using DNA microarray. Materials and methods: Microgrooves were applied on grade II titanium discs to have 0/$0{\mu}m$ (NE0, control group), 60/$10{\mu}m$ (E60/10, experimental group) of respective width/depth by photolithography. The entire surface of the microgrooved Ti substrata was further acid etched and used as the two experimental groups in this study. Human gingival fibroblasts were cultured in the experimental group and the control group, and total RNA was extracted. The oligonucleotide microarray was performed to confirm the changes of various gene expression levels between experimental group and control group. Changes of gene expression level were determined at the pathway level by mapping the expression results of DNA chips, using the KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analysis. Results: Gene expression levels on E60/10 and NE0 were analyzed, there were 123 genes showing significant differences in expression more than 1.5 times on E60/10 microgrooved surface compared to NE0 surface, and 19 genes showing significant differences in expression more than 2 times. The KEGG pathway analysis confirmed the changes in gene expression levels under experimental conditions. Cell signaling, proliferation, and activity among the various gene expression results were identified. Conclusion: Microgrooved surfaces induce gene expression changes and related cell signaling. According to the results of this study, microgrooves can be used as the surface of various biomaterials which need to improve cell activity through gene expression changes and activation of cell signaling.

• Asian-Australasian Journal of Animal Sciences
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• v.19 no.7
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• pp.931-937
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• 2006

The full-length cDNA of the porcine SMPX gene was obtained by the rapid amplification of cDNA ends (RACE). The nucleotide sequences and the predicted protein sequences share high sequence identity with both human and mouse. The promoter of SMPX was sequenced and then analyzed to find the promoter binding sites. The reverse transcriptase-polymerase chain reaction (RT-PCR) revealed that SMPX has a high level of expression in heart and skeletal muscle, a very low expression in lung and spleen and no expression in liver, kidney, fat and brain. Moreover, SMPX has a differential expression level in skeletal muscle, the expression in 65-day embryos being higher than other stages. The porcine SMPX was mapped to SSCXp24 by using a somatic cell hybrid panel (SCHP) and was found closely linked to SW1903 using the radiation hybrid panel IMpRH. An A/G single nucleotide polymorphism (PCR-RFLP) in the 3'-untranslated region (3'-UTR) was detected in eight breeds. The analysis of allele frequency distribution showed that introduced pig breeds (Duroc and Large White) have a higher frequency of allele A while in the Chinese indigenous pig breeds (Qingping pig, Lantang pig, YushanBlack pig, Large Black-White pig, Small Meishan) have a higher frequencies of allele G. The association analysis using an experimental population (188 pigs), which included two cross-bred groups and three pure-blood groups, suggested that the SNP genotype was associated with intramuscular fat content.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.27 no.10A
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• pp.962-968
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• 2002

Reliable high-speed data communications over insufficient channel bandwidth is one of the major challenges of harsh wireless environments that push the achievable spectral efficiency far below its theoretical limits. A Quadrature amplitude modulation (QAM) scheme is a userful modulation technique for achieving high data rate transmission without increasing the bandwidth of wireless communication systems. The exact general bit error rate (BER) expression of arbitrary rectangular quadrature amplitude modulation has not yet been derived. In this paper, a generalized closed-form expression for the BER performance of rectangular QAM with Gray code bit mapping is derived and analyzed in the presence of additive white Gaussian noise (AWGN) channel. First we analyze the BER performance of an I-ary PAM scheme. Regular patterns in the k-th bit error probability are observed while developing the EBR expression. From these patterns we provide the exact and general closed-from EBR expression of an I-ary PAM. Then we present a general closed-from expression for BER of an arbitrary IXJ rectangular QAM by considering that this signaling format consists of two PAM scheme, i.e., I-ary and J-ary PAM. A simple approximate BER expression for rectangular QAM is given as well.

• Journal of the Korea Computer Graphics Society
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• v.22 no.2
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• pp.11-19
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• 2016

This paper proposes a method to directly retarget facial motion capture data to the facial rig. Facial rig is an essential tool in the production pipeline, which allows helping the artist to create facial animation. The direct mapping method from the motion capture data to the facial rig provides great convenience because artists are already familiar with the use of a facial rig and the direct mapping produces the mapping results that are ready for the artist's follow-up editing process. However, mapping the motion data into a facial rig is not a trivial task because a facial rig typically has a variety of structures, and therefore it is hard to devise a generalized mapping method for various facial rigs. In this paper, we propose a data-driven approach to the robust mapping from motion capture data to an arbitary facial rig. The results show that our method is intuitive and leads to increased productivity in the creation of facial animation. We also show that our method can retarget the expression successfully to non-human characters which have a very different shape of face from that of human.

• Korean Institute of Interior Design Journal
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• v.13 no.2
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• pp.64-71
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• 2004

This study attempts to clarify inter-relationship between design expression in the surface of contemporary multi-family housing and physical trends in this digital age. The study examines characteristics of housing design influenced from new digital technology in the forms of metaphor such as dematerialization, fluidity and mobility. Currently constructed multi-family housing in Europe after 1990 has been analyzed to find digital reflections in the surfaces. As a conclusion, findings are as follows; In this digital age, a house is considered as a base in the forms of boxes and containers to network to outer worlds. In a complex of multi-family housing, public spaces move to the inside and become parts of private domain. Therefore, a house directly faces a street/outer world without transitional areas and devices. The facades of housing become delicate skins to control private domain in the inside and the world in the outside. In this digital age, the surfaces of housing are designed by ways of wrapping and screening. Printing and mapping methods are used to surface design because they are manipulative and flexible methods in design like graphic design. Screening devices using louvers and shutters are powerful tools to make random and unpredictable images. If units of a louver and a shutter were pixels, a whole surface would be a digital screen. In such assumption, the facade of buildings reflects digital screen to interface while the louvers in operation reflect clicking to network. As a conclusion, design expression in surfaces in-between of multi-family housing is a metaphor of digital mechanism.