• Cartoon and Animation Studies
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• s.31
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• pp.249-266
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• 2013

The purpose of this research is to study a case of the minimalism on Game Graphic User Interface design in Computer game. It is to analyse the minimal tendency on Game Graphic User Interface design in several Computer games such as Prince of Persia, Mirror's Edge, Splinter Cell, Gears of War, Call of Duty4, Metro 2033, Crysis2. These games expanded the field of Game Graphic User Interface design with the unique form of minimalism. Minimalism came from the formative arts in the 1960s and influenced to other fields of art. It seems to be an expression of various styles that promptly meet the needs of the times. Therefore, in this research, I would highlight the phenomenon of minimalisation in Game Graphic User Interface design and research the influence of Minimalism that appears in modern design focused on "simplicity" and "minimum" which are representative trend of Minimalism.

• Journal of Microbiology and Biotechnology
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• v.3 no.3
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• pp.146-155
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• 1993

A DNA fragment from an alkali-tolerent Bacillus sp., conferring strong promoter activity, was subcloned into the promoter probe plasmid pPL703 and the nucleotide sequence of this promoter region was determined. The sequence analysis suggested that this highly efficient promoter region containing the complex clustered promoters comprised three kinds of promoters (P1, P2 and P3), which are transcribed by $\sigma^B (formerly \sigma^{37}), \sigma^E(formerly \sigma^{29}) and \sigma^A (formerly \sigma^{43})$ RNA polymerase holoenzymes which play major rules at the onset of endospore formation, during sporulation and at the vegetative phase of growth, respectively. S1 nuclease mapping experiments showed that all three promoters had staggered transcription initiation points. The results of chloramphenicol acetyltransferase assay after the subcloning experiments also indicated that the expression of these clustered promoters was correlated with the programs of growth and endospore development. Promoter P1, P2 and P3 were preceded by 75% AT, 79% AT and 81% AT regions, respectively, and a partial deletion of AT-rich region prevented transcription from promoter P1 in vivo. Two sets of 5 -AGTGTT-3 sequences and inverted repeat sequences located around the promoter P1 were speculated as the possible cis acting sites for the catabolite repression in B. subtilis. In vivo transcripts from these sequence regions may be able to form a secondary structure, however, the possibility that a regulatory protein induced by the excess amount of glucose could be bound to such a domain for crucial action remains to be determined.

• The Korean Journal of Physiology and Pharmacology
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• v.17 no.4
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• pp.331-338
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• 2013

AMP-activated protein kinase (AMPK), an important regulator of energy metabolism, is activated in response to cellular stress when intracellular levels of AMP increase. We investigated the neuroprotective effects of AMPK against scopolamine-induced memory impairment in vivo and glutamate-induced cytotoxicity in vitro. An adenovirus expressing AMPK wild type alpha subunit (WT) or a dominant negative form (DN) was injected into the hippocampus of rats using a stereotaxic apparatus. The AMPK WT-injected rats showed significant reversal of the scopolamine induced cognitive deficit as evaluated by escape latency in the Morris water maze. In addition, they showed enhanced acetylcholinesterase (AChE)-reactive neurons in the hippocampus, implying increased cholinergic activity in response to AMPK. We also studied the cellular mechanism by which AMPK protects against glutamate-induced cell death in primary cultured rat hippocampal neurons. We further demonstrated that AMPK WT-infected cells increased cell viability and reduced Annexin V positive hippocampal neurons. Western blot analysis indicated that AMPK WT-infected cells reduced the expression of Bax and had no effects on Bcl-2, which resulted in a decreased Bax/Bcl-2 ratio. These data suggest that AMPK is a useful cognitive impairment treatment target, and that its beneficial effects are mediated via the protective capacity of hippocampal neurons.

• The Journal of the Korea Contents Association
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• v.18 no.3
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• pp.636-647
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• 2018

This study is to explore the characteristics of contemporary Chinese architecture from the aspects of the regionalism of environment, culture and technology. Six cases of contemporary Chinese architecture which won the world-wide famous were selected and analyzed. The results are the followings; First, the regionalism of environment emphasizes the building's response to the regional environment (topography, climate and regional landscape). Second, the regionalism of culture reflects as the relevance between architecture and community structure, economic patterns, folk customs and other social factors of the region. Third, the regionalism of technology requires the coordination of construction process, structure form, materials and other contents of architecture with the regional environment and culture, and emphasizes the suitability of technology. Environment, culture and technology determine that the regionalism of contemporary Chinese architecture is not purely the meaning at the formal level, but explores the underlying causes.

• The Journal of the Korea Contents Association
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• v.18 no.5
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• pp.46-57
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• 2018

Fashion is a cultural element expressing general and common aesthetic awareness in a relevant region in the relevant times. On the aspect that shoes are also a part of fashion reflecting the unique aesthetic awareness of Korea, this paper aims to analyze the aesthetic awareness contained in the aesthetic features observed in the traditional boots. The aesthetic features in terms of the form were the balanced usage of each element including curves, straight lines, simple shapes, contrast colors and materials, and the application and ideological significance as the symbol of social position and status in terms of the contents. Aesthetic awareness pursues the beauty of moderation through the compromise between curves and straight lines, as both elements accept each other's features and keep balance without inclination to one element. Next, solid and void are naturally expressed by appropriately adjusting the expression on colors, materials and shapes through the harmony of composure and techniques. While traditional boots were used as the sign indicating social position and status as the unity of spirit and a thing, they pursue the value of rhythmic vitality by giving spiritual meaning to a thing.

• Journal of Convergence for Information Technology
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• v.10 no.10
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• pp.24-31
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• 2020

In non-orthogonal multiple access (NOMA), the degraded performance of the weaker channel gain user is a problem. In this paper, we propose the asymmetric binary pulse amplitude modulation (2PAM), to improve the bit-error rate (BER) performance of the weaker channel user in NOMA with the tolerable BER loss of the stronger channel user. First, we design the asymmetric 2PAM, calculate the total allocated power, and derive the closed-form expression for the BER of the proposed scheme. Then it is shown that the BER of the weaker channel user improves, with the small BER loss of the stronger channel user. The superiority of the proposed scheme is also validated by demonstating that the signal-to-noise ratio (SNR) gain of the weaker channel user is about 10 dB, with the SNR loss of 3 dB of the stronger channel user. In result, the asymmetric 2PAM could be considered in NOMA of 5G systems. As a direction of the future research, it would be meaningful to analyze the achievable data rate for the propsed scheme.

• Proceedings of the Materials Research Society of Korea Conference
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• 2011.05a
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• pp.53.1-53.1
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• 2011

Bioactive glass powders were synthesized by hydrothermal chemical route by the use of ultrasonic energy irradiation. We used sodalime, calcium nitrate tetra hydrate and di ammonium hydrogen phosphate as the precursor material to synthesize $SiO_2$ rich bio-active glass materials. The $SiO_2$ content was varied in the precursor mixture to 60, 52 and 45 mole%. Dense compacts were obtained by microwave sintering at $1,100^{\circ}C$. Mechanical properties were characterized for the fabricated dense bioactive glasses and were found to be comparable with conventional CaO-$SiO_2$-$Na_2O$-$P_2O_5$ bioactive glass. Detailed biocompatibility evaluation of the glass composition was investigated by in-vitro culture of MG-63 cell and mesenchyme stem cell. Cell adhesion behavior was investigated for both of the cell by one cell morphology for 30, 60 and 90 minutes. Cell proliferation behavior was investigated by culturing both of the cells for 1, 3 and 7 days and was found to be excellent. Both SEM and confocal laser scanning microscopy were used for the investigation. Western blot analysis was performed to evaluate the bimolecular level interaction and extent and rate of specific protein expression. The ability to form biological apatite in physiological condition was observed with simulated body fluid (SBF). In-vivo bone formation behavior was investigated after implanting the materials inside rabbit femur for 1 and 3 month. The bone formation behavior was excellent in all the bioglass compositions, specially the composition with 60% $SiO_2$ content showed most promising trend.

• Journal of Korean Society on Water Environment
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• v.28 no.2
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• pp.313-319
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• 2012

Various nanomaterials may flow into the aquatic ecosystem via production, use, and treatment processes. Especially, gold nanoparticles (AuNPs) were categorized as manufactured nanomaterials presented by the Organization for Economic Cooperation and Development Working Party on Manufactured Nanomaterials (OECD WPMN) in 2010. AuNPs have been used in medical area, however, they were reported to induce cytotoxicity and oxidative DNA damage, as well as down-regulation of the DNA repair gene in mice and human cell lines. In this study, the aquatic toxicity data of AuNPs and gold ions were collected, with the specific test methods analyzed with respect to the form and size of AuNPs, test species, exposure duration, and endpoints. Currently, aquatic toxicity data of AuNPs and gold ions have been presented in 14 studies including 4 fish, 6 crustacean, 2 green algae, and 2 macrophytes studies, as well as a further 8 studies including 4 fish, 4 crustacean, 1 platyhelminthes, and 1 green algae, respectively. The AuNPs were 0.8-100 nm in size, as gold nanoparticles, gold nanorod, glycodendrimer-coated gold nanoparticles, and amine-coated gold nanoparticles. The tested endpoints were the individual toxicities, such as mortality, malformation, reproduction inhibition, growth inhibition and genetic toxicity such as oxidative stress, gene expression, and reactive oxygen species formation. The accumulation of AuNPs was also confirmed in the various receptor organs. These results are expected to be useful in understanding the aquatic toxicity of AuNPs and gold ions, as well as being applicable to future toxicity studies on AuNPs.

• The Journal of the Petrological Society of Korea
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• v.28 no.4
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• pp.307-317
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• 2019

Petrological studies were performed on the Sanbangsan lava dome, located in the southwest of Jeju Island Volcanic Field. According to the lava ejection method, it is 'an internal primitive form' that is gradually pushed up and expanded by continuous magma injection from the bottom to the top of the vent and it corresponds to the 'low lava dome'. The rocks are partly plotted in the field of benmoreite, but mostly plotted in the field of trachyte of the Cox et al.(1979) classification diagram, and also mainly plotted in the field of trachyte of Le Maitre et al.(2002) and Zr/TiO₂-Nb/Y classification diagram. Therefore, the expression that described the rock of Sanbangsan lava dome as 'trachy-andesite' should be corrected to 'trachyte'. The volcanic rocks that consists in the Sanbangsan lava dome are trachyte containing normative quartz and shows differentiation trend in the range of 59.75-63.46 wt.% SiO₂.

• Korean Journal of Microbiology
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• v.41 no.2
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• pp.93-98
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• 2005

The sphingosine kinase gene, which is 969-nucleotide long, was identified during the whole genome sequencing of Sphingomonas chungbukensis DJ77. The amino acid sequence showed the identity of $55\%$ with that of Zymomonas mobilis subsp. mobilis ZM4. C2, C3, and C5 domains of eukaryotic sphingosine kinase were found in sphingosine kinase from Sphingomonas chungbukensis DI77. One of these three conserved sites, GGDG, was predicted as a ATP-binding site, and the functions of the others were unknown currently. The phylogenetic tree constructed by ClustalX indicated that the sphingosine kinase of S. chungbukensis DJ77 was near the phylogenetic group COG1597, and did not belong to the group of diacylglycerol kinase of the same strain. The recombinant sphingosine kinase was expressed in Escherichia coli, but it was made in form of inclusion body.