• Title/Summary/Keyword: Experiments

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Pie-establishment of Microwave-Assisted Extraction Conditions for Antioxidative Extracts from Cabbage (양배추의 항산화성 추출물 제조를 위한 마이크로웨브 추출조건 설정)

  • Noh Jungeun;Choi You-Kyoung;Kim Hyun-Ku;Kwon Joong-Ho
    • Food Science and Preservation
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    • v.12 no.1
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    • pp.62-67
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    • 2005
  • Microwave-assisted extraction (50 W, 2,450 MHz, MAE) with properties of selective heating and subsequent extraction for certain phytochemicals from natural materials was applied to pre-establish the extraction conditions for total yield total phenolics, and electron donating ability (EDA) from Brossica oleacea. The experiments with $50\%$ EtOH solvent showed that 20 mesh in particle size of cabbage flake $(moisture\;4.5\%)$ and 1:10 (g/mL) in the sample to solvent ratio for both raw $(moisture\;90.2\%)$ and flake cabbages were optimal for MAE efficiency. Under these conditions, total yield increased with extraction tim, which was highest for raw cabbage extract in $50\%\;EtOH$ solvent followed by $100\%\;EtOH$ and water. While that of flake cabbage extracts was highest in $50\%\;EtOH$ followed by water and $100\%\;EtOH$. The contents of total phenolics and EDA in extracts gradually increased after 3 min of MAE, which were highest when using $100\%\;EtOH$ solvent followed by $50\%\;EtOH$ and water in raw cabbage and $50\%\;EtOH$ followed by water and $100\%\;EtOH$ in flake cabbage, respectively.

Optimization of microwave-assisted extraction process of Hordeum vulgare L. by response surface methodology (반응표면분석법을 이용한 새싹보리 마이크로웨이브 추출공정의 최적화)

  • Lee, Jae-Jun;Park, Dae-Hee;Lee, Won-Young
    • Food Science and Preservation
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    • v.24 no.7
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    • pp.949-956
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    • 2017
  • This study attempted to find optimum extract range of active ingredient for barley sprouts (Hordeum vulgare L.). Extracts from Hordeum vulgare L. were made by microwave extraction method and total polyphenol content (TPC), total flavonoid content (TFC), DPPH radical scavenging activity (DPPH) were measured with extract of Hordeum vulgare L.. Response surface methodology (RSM) was applied to a extraction process, and central composite design (CCD) was also used for this process to examine the optimum condition. Independent variables ($X_n$) are concentration of ethanol ($X_1$: 0, 25, 50, 75, 100%), microwave power ($X_2$: 60, 120, 180, 240, 300 W), extraction time ($X_3$: 4, 8, 12, 16, 20 min). Dependent variables ($Y_n$) are TPC ($Y_1$), TFC ($Y_2$), DPPH radical scavenging ($Y_3$). It is formed by sixteen conditions to extract. The $R^2$ value of dependent variables is ranged from 0.90 to 0.97 (p<0.05). Experiments values within the optimal range (40% of ethanol concentration, 120 W of microwave power, 18 min of extraction time) were 3.74 mg GAE/g (TPC), 3.00 mg RE/g (TFC), 35.43% (DPPH), respectively. Under the optimized conditions, predicted value showed no significant difference comparing with the experimental values.

Quality characteristics of Weissella confusa strain having gluten degradation activity from salted seafood (젓갈로부터 분리된 글루텐 분해능을 가지는 Weissella confusa 균주와 특성)

  • Yoon, Jong Young;Hwang, Kwontack
    • Food Science and Preservation
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    • v.23 no.6
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    • pp.883-889
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    • 2016
  • A new lactic acid bacteria with gluten-degrading activity which was isolated from salted sea foods (traditional Korea fermented food), identified as Weissella confusa (99%) by use of API kit and 16S rRNA sequencing, and designated as W. confusa. When the W. confusa cultured for 48 hours at $30^{\circ}C$ in a MRS medium containing 1% gluten, 45% of gluten was founded to be degraded. W. confusa showed 85% of survival rate at pH 3, and 94% tolerance at 0.1% oxgall, which indicates that W. confusa would survive in stomach of human. Experiments on the thermostability was confirmed that it has a stability of 70% in $50^{\circ}C$. W. confusa inhibited the growth of some pathogen, except for S. aureus. Results in this study suggest that using W. confusa for fermentation of grain flour containing gluten would be desirable to prepare the gluten-free foods needed for those who suffer from celia disease and gluten allergy.

Spatial and temporal variation on fruit set in Epipactis thunbergii (Orchidaceae) from southern Korea (한국남부 자생 닭의난초 (난초과)의 시 공간에 따른 결실률 변이)

  • Chung, Mi Yoon;Chung, Myong Gi
    • Korean Journal of Plant Taxonomy
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    • v.45 no.4
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    • pp.353-361
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    • 2015
  • Spatio-temporal variation in fruit set in orchids would affect long-term population viability and will influence genetic diversity over many generations. The aim of this study was to examine the breeding system of the nectariferous terrestrial orchid Epipactis thunbergii, to specifically determine levels of fruit set in terms of time and space under natural conditions. We examined pollination under natural conditions and conducted hand pollination experiments during a 2-year survey in four populations located along 1.5 km of coastal line in Jinguiri (rual village) [Jeollanam-do (province), southern Korea]. We found that, over a 2-year period, levels of percentage of fruit set were similar within patches of the four populations. By contrast, we detected significant differences in the percentage of fruit set among patches. We also found that plants with larger inflorescence size produced significantly more fruits than plants with fewer flowers. Over a 2-year period, the percentage of fruit set for E. thunbergii was similar but low (14.1%) compared to that averaged for eighty-four rewarding species (37.1%). However, an increase in fruit set was achieved by hand-pollinations: artificial self-pollination (90.5-95.2%), artificial geitonogamy (94.7-95.0%), and cross-pollination (artificial xenogamy, 91.3-91.4%). No emasculated flowers produced fruits and no automatic pollination was found in E. thunbergii. Our findings suggest that E. thunbergii is a self-compatible terrestrial orchid that depends on pollinators (insects) to achieve fruit set in natural habitats, and that local environmental conditions were similar over a period of 2 years in the study area. Our results also highlight the cryptic variation of fruit production in time, but more pronounced variability in space.

Hand Gesture Recognition Regardless of Sensor Misplacement for Circular EMG Sensor Array System (원형 근전도 센서 어레이 시스템의 센서 틀어짐에 강인한 손 제스쳐 인식)

  • Joo, SeongSoo;Park, HoonKi;Kim, InYoung;Lee, JongShill
    • Journal of rehabilitation welfare engineering & assistive technology
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    • v.11 no.4
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    • pp.371-376
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    • 2017
  • In this paper, we propose an algorithm that can recognize the pattern regardless of the sensor position when performing EMG pattern recognition using circular EMG system equipment. Fourteen features were extracted by using the data obtained by measuring the eight channel EMG signals of six motions for 1 second. In addition, 112 features extracted from 8 channels were analyzed to perform principal component analysis, and only the data with high influence was cut out to 8 input signals. All experiments were performed using k-NN classifier and data was verified using 5-fold cross validation. When learning data in machine learning, the results vary greatly depending on what data is learned. EMG Accuracy of 99.3% was confirmed when using the learning data used in the previous studies. However, even if the position of the sensor was changed by only 22.5 degrees, it was clearly dropped to 67.28% accuracy. The accuracy of the proposed method is 98% and the accuracy of the proposed method is about 98% even if the sensor position is changed. Using these results, it is expected that the convenience of the users using the circular EMG system can be greatly increased.

Study on Indoor Wireless Environment of mmWave WLAN Communication (초고주파 근거리 통신의 실내 무선 환경 연구)

  • Shin, Dong-Il;Kim, Woo-Seong;Park, Yang-Jae
    • Journal of Digital Convergence
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    • v.16 no.1
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    • pp.147-152
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    • 2018
  • Recently, as the demand for transmission of ultra-high quality media data such as UHD, AR, and VR increases, various technologies for this have been actively developed and IEEE 802.11ad standard have been commercialized. In this paper, a test bed is constructed to analyze the indoor wireless environment using the IEEE 802.11ad standard based on mmWave, and the experimental results of various indoor wireless environments are introduced and analyzed. We compared the data from the module by data transmission, such as signal to noise ratio(SNR) and throughput. And we measured the beam pattern and width of the module and compared the effects on the indoor environment of the corridor and the office. This shows that the signal reflection of the wall shows higher SNR values and is more suitable to use for indoor than outdoor. It is confirmed that the loss when not in line of sight(LoS) is not enough to compensate the wall reflected signal. As a result, it is judged to be suitable for the indoor use of the mmWave LAN and can be usefully used for further experiments.

Development of Functional Tea Products for Hypertension Patients with the Mixed Medicinal Herbal Extracts (고혈압 환자에 도움을 줄 수 있는 한방 다류 개발)

  • Ahn, Byung-Yong
    • Journal of the East Asian Society of Dietary Life
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    • v.19 no.6
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    • pp.1049-1053
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    • 2009
  • This study was carried out to investigate the effects of Eucommia ulmoides Oliver (Du-Zhong) as a main ingredient in a mixture of various medicinal herbal extracts on blood pressure, serum lipid concentrations, and hematological variables in hypertensive patients. After 4 weeks on drinking the mixed medicinal herbal teas, systolic blood pressure remarkably decreased from 167.14 to 134.86 mmHg and diastolic blood pressure decreased from 100.00 to 87.10 mmHg. In terms of serum lipid profiles, there were no differences in total-cholesterol, HDL-cholesterol, LDL-cholesterol level, and atherogenic index, However, the level of triglycerol was significantly reduced from 237.1 before the experiment to 145.00 mg/dL after 4 weeks on drinking the mixed medicinal herbal teas. Serum triglyceride were remarkably reduced from 237.1 to 145.00 mg/dL in the hypertensive patients who had taken the combined medicinal herbal extracts. Compared with data obtained from the patients before the experiment, there were no differences in hematological variables (RBC, WBC, hematocrit, hemoglobin, platlets and homocysteine) after the 4 weeks experiments, but mean corpuscular hemoglobin concentration was significantly increased by 2.70% (p<0.05) in the blood samples. Based on these findings, it can be presumed that the examined mixed medicinal herbal tea may be effective in lowering blood pressure and in increasing mean corpuscular hemoglobin concentration due to reduced serum triglyceride levels in hypertension patients.

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A Study on the Habitat Environment and Mutualism with Ants of Genus Melampyrum - focusing on M. roseum, M. setaceum var. nakaianum and M. roseum var. ovalifolium in Kangwondo - (며느리밥풀속 서식지 특성 및 개미와의 공생관계에 관한 연구 - 강원도의 꽃며느리밥풀, 새며느리밥풀 및 알며느리밥풀을 중심으로 -)

  • Lee, Jae-Hyeon;Lyu, Dong-Pyo;Kim, Gab-Tae
    • Korean Journal of Environment and Ecology
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    • v.30 no.2
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    • pp.139-145
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    • 2016
  • To search for habitat characteristics of myrmecochory Melampyrum species, diaspores and related ant species, habitat characteristics, collecting fruits and diaspores of three Melampyrum species, cafeteria experiments, and the related ant species were studied from May 2012 to November 2014. The habitat of Melampyrum species was south-facing open forest edge with slightly acidic soil and relatively shallow soiil layers. Major plants coexisting with Melampyrum species were four woody species including Quercus mongolica and four herbaceous species including Carex siderosticta. These Plants might be host plants of the Melampyrum species. The shape of the diaspore was an oval seed attached to the white cap on one side. Significant differences is proved between three plant species in weight of diaspores, seeds and and elaiosome ratios. Mean values of diaspores weight showed 10.6mg, 8.0mg and 7.2mg in M. setaceum var. nakaianum, M. roseum var. ovalifolium and M. roseum, respectively. The heaviest elaiosome is M. roseum var. ovalifolium with 1.2mg, M. setaceum var. nakaianum has 1.0mg and the lightest elaiosome is M. roseum with 0.8mg. Major ant species frequently transporting the diaspores of Melampyrum species are Myrmicinae four species; Myrmica kotokui, M. excelsa, Myrmecina nipponica and Aphaenogaster japonica. Further studies on Melampyrum species, hemiparasite annual myrmecochorous plant, and related ant species should be needed.

Effect of Salinity on the Survival and Growth of Larvae of the Boreal Digging Frog (Kaloula borealis) (맹꽁이 유생의 생장과 생존에서의 염분영향)

  • Ko, Sang-Beom;Ko, Young-Min;Lee, Jeong-Hyun
    • Korean Journal of Environment and Ecology
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    • v.29 no.4
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    • pp.533-538
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    • 2015
  • This study was conducted to examine the effect of salinity on the early embryonic development stage of boreal digging frogs (Kaloula borealis). An experiment was carried out with the eggs of K. borealis mating couples laid along the coastal zone of Jeju Island in June 2013. The salinity was set to 0, 1, 3, 5, and 7 ‰, respectively. The survival and growth of the larvae at various stages from the egg phase to the closing of their external gills phase were observed. In another experiment, tadpoles in internal gill stage were placed in solutions with salinity of 0, 1, 3, 5, and 7 ‰, respectively, and their survival rate and growth rate were observed, and then the results of the two experiments above were compared. The results showed that in egg and external gill stage, there was no significant difference in survival rate in 3 ‰ solution, though there was low survival rate in 5 ‰ solution. Further, all the eggs were dead in 7 ‰ solution, so it appeared that solutions with salinity of 5 ‰ or higher affect the survival of K. borealis in the early embryonic development. Larvae in the internal gill stage showed no significant difference in survival rate from the control group up to a salinity of 5 ‰, but when placed in a solution of over 7 ‰ salinity, the survival rate decreased. The growth rate also slowed down with the higher salinity. It appeared that in both the external gill stage and the internal gill stage in 5 ‰ solution, the growth rate decreased significantly compared to the control group. From this study it is concluded that higher salinity values have a significant impact on the survival and growth of the K. borealis larvae and this finding can be used to conserve K. borealis species that are decreasing in number due to the side effects of development in coastal areas.

Characteristics of Isotherm, Kinetic, and Thermodynamic Parameters for Reactive Blue 4 Dye Adsorption by Activated Carbon (활성탄에 의한 Reactive Blue 4 염료의 흡착에 대한 등온선, 동력학 및 열역학적 특성)

  • Lee, Jong-Jib
    • Clean Technology
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    • v.26 no.2
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    • pp.122-130
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    • 2020
  • The isotherm, kinetic, and thermodynamic parameters of reactive blue 4 adsorbed by activated carbon were investigated for activated carbon dose, pH, initial concentration, contact time, and temperature data. The adsorption of the RB 4 dye by activated carbon showed a concave shape in which the percentage of adsorption increased in both directions starting from pH 7. The isothermal adsorption data were applied to Langmuir, Freundlich, and Temkin isotherms. Both Freundlich and Langmuir isothermal adsorption models fit well. From determined Freundlich separation factor (1/n = 0.125 ~ 0.232) and Langmuir separation factor (RL = 1.53 ~ 1.59), adsorption of RB 4 by activated carbon could be employed as an effective treatment method. The constant related to the adsorption heat (BT = 2.147 ~ 2.562 J mol-1) of Temkin showed that this process was physical adsorption. From kinetic experiments, the adsorption process followed the pseudo second order model with good agreement. The results of the intraparticle diffusion model showed that the inclination of the first straight line representing the surface diffusion was smaller than that of the second straight line representing the intraparticle pore diffusion. Therefore, it was confirmed that intraparticle pore diffusion is the rate-controlling step. The negative Gibbs free energy change (ΔG = -3.262 ~ -7.581 kJ mol-1) and the positive enthalpy change (ΔH = 61.08 kJ mol-1) indicated the spontaneous and endothermic nature of the adsorption process, proving this process to be spontaneous and endothermic.