• Title/Summary/Keyword: Experimental research

Search Result 23,510, Processing Time 0.057 seconds

Radioprotective Effect of Red Ginseng in Irradiated Mice with ${\gamma}$-ray (생쥐에서 홍삼의 감마선조사에 의한 방어효과)

  • Seung, Ka-Yeon;Lee, Heung-Man;Kim, Jong-Sang;Lee, Jun-Haeng
    • Journal of the Korean Society of Radiology
    • /
    • v.4 no.1
    • /
    • pp.31-35
    • /
    • 2010
  • Recently, the incidents of direct or indirect radiation exposure due to increase of use of radiation or radioisotope are on the increase in medical and industrial circles. If cells are irradiated, free radicals are created through biological process, and cells are directly or indirectly damaged. This research intends to explore into the effect of saponin at the level of cell (in vitro) and entity (in vivo), using red ginseng extract "saponin", as radioprotective agent. In the experiment implemented at the level of cell (in vitro), degree of cell activity was measures by adding mouse mesenchymal stem cells "C3H/10T1/2 cells" into red ginseng extract "saponin(0, 0.05, 0.2, and 0.4 g/L)", and then the optimal concentration of saponin influencing cells was calculated, in 24, 48, 72, and 96 hours after gamma irradiation at the optimal concentration of saponin, each cell survival rate was observed through XTT assay. The best time period of cultivation for the optimal activity of C3H/10T1/2 cells was as 48 hours, and the degree of optimal activity was shown at 0.05 g/L. In 48 hours after irradiation of 5 Gy to C3H/10T1/2 cells at 0.05 g/L, the degree of activity of cells increased by 10%. In the experiment implemented at the level of entity (in vivo), red ginseng extract "saponin" at a dose of 100 mg/kg/day was injected into the abdominal cavity of six-week immature mouse for two weeks. Right after the last abdominal injection, total body irradiation of gamma rays was carried out at a dose of 5 Gy and 10 Gy. And after irradiation, the blood sample was taken, and then the number of red corpuscles was counted. In result, the decrement of experimental group treated with red ginseng extract "saponin" was 2.3 times larger than that of control group. In view of the results so far achieved, it was revealed that red ginseng extract "saponin" has a radiation exposure protection effect in the experiment implemented at the level of cell (in vitro). In case of animal experiment, the decrement of number of red corpuscles decreased. Finally, it is necessary to carry out more various researches continuously.

Influences of Seleniferous Whole Crop Barley Supplement on Growth Performance and Blood Characteristics in Growing Pigs (셀레늄함유 청보리 급여가 육성돈의 생산성 및 혈액성상에 미치는 영향)

  • Lee, Sung-Hoon;Jo, Ik-Hwan;Kim, Guk-Won;HwangBo, Soon;Han, Ouk-Kyu;Song, Tae-Hwa;Park, Tae-Il;Choi, In-Bae
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.32 no.1
    • /
    • pp.39-48
    • /
    • 2012
  • This study was conducted to investigate effects of different levels of seleniferous whole crop barley (WCB) supplementation on performance, and blood characteristics as physiological responses in growing pigs. A total of 20 cross-bred pigs ((Landrace ${\times}$ Yorkshire) ${\times}$ Duroc) were divided into 4 treatments of 5 pigs each and experimental period lasted for 6 weeks. They were fed diets containing 0.1 (non-seleniferous WCB as controls), 0.2, 0.4, and 0.6 mg/kg levels of selenium (Se) by supplementing seleniferous WCB, and non-seleniferous or seleniferous WCB was formulated to 5% level in total ration. The diets were isonitrogenous (18% crude protein) and isocaloric (3,500 kcal/kg digestible energy) across treatments. Increasing levels of seleniferous WCB supplements did not affect feed intake and BW gain, and blood total protein concentration was (p<0.05) significantly higher for 0.2 mg/kg Se treatments than for controls. On d 14, blood albumin concentration was higher (p<0.05) for seleniferous WCB supplemented groups than for control group. Contrarily, blood glucose concentration was tended to be higher for controls than for seleniferous WCB groups. Blood total lipid concentration was significantly (p<0.05) lowered with increasing levels of seleniferous WCB. Serum glutamic-oxaloacetic transaminase and glutamic-pyruvic transaminase did not have any difference among treatments. It was tended that blood total cholesterol and triglyceride were lowered with increasing levels of seleniferous WCB. Blood Se concentration was significantly (p<0.05) increased with increasing levels of seleniferous WCB. The results indicate that Se present in seleniferous WCB had favorable effects on blood characteristics and blood Se increased by supplementing seleniferous WCB implies not only a good intestinal absorption of Se present in WCB but also the possibility of Se transfer into tissues.

Effect of Seleniferous Whole Crop Barley Silage on Growth Performance, Blood and Carcass Characteristics, and Tissue Selenium Deposition in Finishing Hanwoo Steers (셀레늄 강화 청보리 사일리지 급여가 비육기 거세한우의 생산성, 혈액성상 및 도체특성과 조직 내 셀레늄 축적에 미치는 영향)

  • Hwangbo, Soon;Jo, Ik Hwan;Kim, Guk Won;Choi, Chang Weon;Lee, Sung Hoon
    • Journal of The Korean Society of Grassland and Forage Science
    • /
    • v.33 no.4
    • /
    • pp.281-289
    • /
    • 2013
  • This study was conducted to investigate the effects of feeding seleniferous whole crop barley silage (WCBS) on the growth performance, blood and carcass characteristics, and tissue selenium deposition infinishing Hanwoo steers. A total of 20 growing Hanwoo steers were selected and assigned to one of the following feed groups: control (0.1 ppm Se), T1 (0.3 ppm Se), T2 (0.9 ppm Se), and T3 (0.9 ppm of inorganic Se). Five steers were allocated to each treatment group, and the trial lasted for 90 days. All experimental diets combined seleniferous and/or non-seleniferous WCBS up to a ratio of 30%. For the T3 diet, 0.9 ppm Se in the form of sodium selenite was added to the control diet. Dietary level and type of selenium did not affect feed intake and body weight gain. Blood total lipid and triglyceride concentrations were significantly (p<0.05) lower in the T2 group than in control. Blood immunoglobulin G concentration increased significantly (p<0.05) with increasing levels of dietary selenium; concentrations were significantly (p<0.05) higher in T2 and T3 than in control. Blood selenium concentration was the highest (p<0.05) in T2. No significant difference was observed in dressing rate, ribeye area, marbling score, meat color and fat color. Further, no association was found between levels and chemical form of dietary selenium and quality and quantity of meat. To the contrary, tissue selenium content in kidney, liver, and hind leg increased significantly (p<0.05) with increasing levels of selenium; however, feeding inorganic selenium did not introduce a significant increase in tissue selenium content of finishing Hanwoo steers. The results indicated that the selenium present in seleniferous WCBS was an effectively absorbable selenium source, suitable for increasing tissue selenium content in beef. Approximately 100 g of hind leg muscle from steers fed on the T2 diet met approximately 69% of the recommended daily selenium requirements.

Ultasonic Reflection Characteristics of the Underwater Corner Reflector (수중코오너리프렉터의 초음파반사특성에 관한 연구)

  • Lee, Dae-Jae;Sin, Hyeong-Il
    • Journal of the Korean Society of Fisheries and Ocean Technology
    • /
    • v.19 no.1
    • /
    • pp.25-32
    • /
    • 1983
  • The corner reflector is used to increase the echoing area of radar targets in the air, and it can also be applied to increase the echoing area of the sonar targets under water. As the basic research for this application, the authors investigated the ultrasonic reflection characteristics under water for the corner reflector which was made of aluminum plate. The experiments were made by pulse measuring method with the magnetostrictive ferrite transducers of 28, 50 and 75KHz in the experimental water tank. The results obtained are as follows; 1. The target strength of corner reflectors were increased in proportion to the diameter and were greater at higher frequency of 75KHz than at lower frequency of 28KHz. 2. In the case of 5 corner reflectors of 150mm in diameter which have corner angles of 15$^{\circ}$, 30$^{\circ}$, 45$^{\circ}$, 60$^{\circ}$ and 90$^{\circ}$the measured values of the maximum target strength at 75KHz were-25.0 dB, -17.2dB, -15.1dB, -13.4dB and 11.0dB, and then the number of main lobes showing the maximum target strength in the backscattering patterns were 24, 12, 8, 6 and 4, respectively. 3. When 7 corner reflector of 80mm in diameter and 90$^{\circ}$ in the corner angle was located on the minor axis of the horizontal section with directional angles of 0$^{\circ}$, 2.5$^{\circ}$, 5.0$^{\circ}$, 7.5$^{\circ}$, 10$^{\circ}$ and 12.5$^{\circ}$ against the sound beam axis, the measured values of the target strength on each position at 75KHz were -21.2dB, -21.9dB, -26.0dB, -30.5dB and -36.8dB, respectively.

  • PDF

Effect of Temperature Pre-conditioning on Fruit Quality of Early-season 'Hanareum' Pears (Pyrus pyrifolia Nakai) during Simulated Marketing (조생종 '한아름' 배 모의유통 전 예건처리 온도가 품질에 미치는 영향)

  • Lee, Ug-Yong;Oh, Kwang-Suk;Hwang, Yong-Soo;Lim, Byung-Sun;Ahn, Young-Jik;Chun, Jong-Pil
    • Horticultural Science & Technology
    • /
    • v.34 no.1
    • /
    • pp.94-101
    • /
    • 2016
  • The purpose of this study was to establish postharvest management techniques including a temperature pre-conditioning protocol for maintaining fruit quality in newly developed early-season Korean pear cultivar 'Hanareum' (Pyrus pyrifolia Nakai). The fruits were treated with three different pre-conditioning temperatures (21, 25, or $29^{\circ}C$) for 4 days according to the harvest time (103 or 110 days after full bloom, DAFB). The percent weight loss was relatively low in the fruits subjected to low pre-conditioning temperature regardless of harvest time. The firmness of the fruits treated with pre-conditioning at $21^{\circ}C$ remained high during 20 days of simulated marketing at $25^{\circ}C$, although all treated fruits showed a general decline of firmness with extended time of simulated marketing. These fruits also showed higher appearance and a lower incidence of mealiness disorder symptoms. During the experimental periods, the production of ethylene was lower in the fruits pre-conditioned at $21^{\circ}C$ in comparison with those of treated at 25 and $29^{\circ}C$. High respiration rates were obvious in the fruits pre-conditioned at high temperature ($29^{\circ}C$), especially in the optimum-harvested fruits, where respiration was approximately two times higher than that of fruits exposed to $21^{\circ}C$ during pre-conditioning. However, the respiration rate was similar during simulated marketing at $25^{\circ}C$ regardless of harvest time. These results demonstrated that temperature pre-conditioning at $21^{\circ}C$ is a simple and effective postharvest technique for summer harvested Korean pear cultivars including 'Hanareum'.

A Single Index Approach for Time-Series Subsequence Matching that Supports Moving Average Transform of Arbitrary Order (단일 색인을 사용한 임의 계수의 이동평균 변환 지원 시계열 서브시퀀스 매칭)

  • Moon Yang-Sae;Kim Jinho
    • Journal of KIISE:Databases
    • /
    • v.33 no.1
    • /
    • pp.42-55
    • /
    • 2006
  • We propose a single Index approach for subsequence matching that supports moving average transform of arbitrary order in time-series databases. Using the single index approach, we can reduce both storage space overhead and index maintenance overhead. Moving average transform is known to reduce the effect of noise and has been used in many areas such as econometrics since it is useful in finding overall trends. However, the previous research results have a problem of occurring index overhead both in storage space and in update maintenance since tile methods build several indexes to support arbitrary orders. In this paper, we first propose the concept of poly-order moving average transform, which uses a set of order values rather than one order value, by extending the original definition of moving average transform. That is, the poly-order transform makes a set of transformed windows from each original window since it transforms each window not for just one order value but for a set of order values. We then present theorems to formally prove the correctness of the poly-order transform based subsequence matching methods. Moreover, we propose two different subsequence matching methods supporting moving average transform of arbitrary order by applying the poly-order transform to the previous subsequence matching methods. Experimental results show that, for all the cases, the proposed methods improve performance significantly over the sequential scan. For real stock data, the proposed methods improve average performance by 22.4${\~}$33.8 times over the sequential scan. And, when comparing with the cases of building each index for all moving average orders, the proposed methods reduce the storage space required for indexes significantly by sacrificing only a little performance degradation(when we use 7 orders, the methods reduce the space by up to 1/7.0 while the performance degradation is only $9\%{\~}42\%$ on the average). In addition to the superiority in performance, index space, and index maintenance, the proposed methods have an advantage of being generalized to many sorts of other transforms including moving average transform. Therefore, we believe that our work can be widely and practically used in many sort of transform based subsequence matching methods.

THE EFFICACY OF PROGRAMMED CRYO-PRESERVATION UNDER PRESSURE IN RAT PERIODONTAL LIGAMENT CELLS (압력 저속 냉동 방법의 쥐 치아 치주인대세포 보존 효율 평가)

  • Lee, Young-Eun;Kim, Eui-Seong;Kim, Jin;Han, Seung-Hoon;Lee, Seung-Jong
    • Restorative Dentistry and Endodontics
    • /
    • v.34 no.4
    • /
    • pp.356-363
    • /
    • 2009
  • The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at $4^{\circ}C$for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in $37^{\circ}C$water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group. By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth.

Changes of Weed Populations Affected by Herbicide Treatment in Apple Fields (제초제(除草劑) 처리(處理)에 의(依)한 과수원(果樹園) 잡초군락(雜草群落)의 변화(変化))

  • Kim, Kil Ung;Shin, Dong Hyun;Cho, Young Woo
    • Current Research on Agriculture and Life Sciences
    • /
    • v.2
    • /
    • pp.34-41
    • /
    • 1984
  • This study was conducted to investigate yearly changes of weeds occurring in orchard and to evaluate an effect of continuous herbicide application on weed population in apple fields. Digitaria sanguinalis, Cephalonoplos segetum, Calystegia hederacea, Polygonum aviculare, Echinochloa crus-galli, and Portulaca oleracea were observed to be the problem weeds in orchard with yearly similar species for a three-year, showing D. sanguinalis was single dominant species for a three-year experimental period. An increase of Simpson's index from 0.22 in the first year to 0.33 in the 3 rd year was observed in the oxyfluorfen treated plot, indicating that most of the weed species were well controlled, while in the untreated plot, D. sanguinalis was relatively single dominant species showing Simpson's index, 0.56 in the 1st year and decreased to 0.32 in the 3 rd year meaning diversification of weed species, and the same trend was also observed in community dominance. Dissimilarity coefficient, 41.6, observed in the weed populations of 1st year and 2nd year, increased up to 58.8 between 1st year and 3 rd year's weed populations in the oxyfluorfen treated plot, indicating that weed community was greatly affected by oxyfluorfen treatment. Based on 3 year's dry weight oxyfluorfen treatment controlled 95 % of weeds such as $149.3g/m^2$ dry weight in the untreated control plot, with a little yearly changes of dry weight. Composition of perennial and biennial weeds was about 59% in the herbicide treated plots and 30% in the untreated control, showing a relative increase of these weeds in the treated plot. However, total dry weight of these weeds was markedly low, showing less than 5% of the untreated one. An annual, D. sanguinalis composed of 70% of total dry weight in the untreated control.

  • PDF

Estimation of Combining Abilities for Traits of Mice from Diallel Crosses -II. Estimation of Combining Abilities for Baby Weights at Weaning and at the Age of 60 Days (양면교잡(兩面交雜)에 의(依)한 Mouse 주요(主要) 형질(形質)의 결합능력(結合能力) 추정(推定) -II. 이유시(離乳時) 체중(體重)과 60일령(日齡) 체중(體重)에 대한 결합능력(結合能力) 추정(推定))

  • Hyun, Byung Hwa;Choi, Kwang Soo
    • Current Research on Agriculture and Life Sciences
    • /
    • v.4
    • /
    • pp.119-123
    • /
    • 1986
  • The study was conducted to find out the gene effects on body weights at weaning and at the age of 60 days in mice, with 343 progenies from full-dialell crosses of four lines of BALB/c, CBA, C3H and C57BL. The progenies were reared at the Experimental Animal Farm, College of Agriculture, Kyungpook National University from November, 1984 to February, 1985, and data collected from the progenies were analyzed into general combining ability, maternal effects, specific combining ability and reciprocal effects with Harvey's model. General combining ability effects estimated in line-crosses were -0.6033~0.5298 for weaning weights and -0.5086~1.0012 for body weights at the age of 60 days. General combining ability for BALB/c and C57BL were significantly better than general combining ability for CBA and C3H for both traits (P<0.05). Maternal effects for C3H were significantly larger than the maternal effects of BALB/c for both traits (P<0.05). The estimates of maternal effects were -0.9678~0.4609 for weaning weights and -1.1886~0.0729 for body weights at the age of 60 days. Specific combining ability effects were estimated to be significant (P<0.05), and the estimates were -0.1999~0.3380 for weaning weights and -0.4056~0.3317 for body weights at the age of 60 days. Reciprocal effects were found to be largest in BALB/c${\times}$C57BL and BALB/c${\times}$C3H. The estimates were -0.5049 from BALB/c${\times}$C57BL and 0.4972 from BALB/c${\times}$C3H form weaning weights, and -1.0336 from BALB/c${\times}$C57BL and 1.2793 from BALB/c${\times}$C3H for body weights at the age of 60 days.

  • PDF

Soil Analysis on Prediction of Consolidation Settlement in Marine Clays (항만점토(港灣粘土)의 압밀심하량(壓密沈下量) 예측(預測)을 위(爲)한 토질분석(土質分析))

  • Kwon, Moo Nam;Son, Kwang Sik;Lee, Sang Ho
    • Current Research on Agriculture and Life Sciences
    • /
    • v.4
    • /
    • pp.87-94
    • /
    • 1986
  • This study was performed in order to contemplate their correlations between physical and mechanical properties of the marine clays which were collected from main harbors in Korea. The results obtained are as follows: 1. Most of the soils in experimental districts consist of CH. CL. and ML. and they are considered to be still proceeding. 2. The equations of the relationship between compression index and liquid limit are as, follows: CH : $C_c=0.0137$ (LL-22.60) CL : $C_c=0.0123$ (LL-14.64) 3. The relationship between compression index and initial void ratio appears that the higher the plasticity, the easier the slope of the regression line. The equations are as follows : CH : $C_c=0.431$ ($e_o-0.504$) CH : $C_c=0.471$ ($e_o-0.235$) ML : $C_c=0.641$ ($e_o-0.393$) 4. The equations of the relationship between compression index and natural water content are as follows: CH : $C_c=0.0133$ ($W_n-28.27$) CL : $C_c=0.0225$ ($W_n-23.56$) ML : $C_c=0.0106$ ($W_n-16.42$) 5. The relationship between initial void ratio and natural water content, and compression index is highly positive correlation and the equations are as follows : CH : $C_c=0.301$ ($e_o+0.017W_n-1.05$) CL : $C_c=0.141$ ($e_o+0.0567W_n-1.054$) ML : $C_c=0.421$ ($e_o+0.0214W_n-1.121$) 6. The equations of the relationship between initial void ratio and liquid limit, and compression index are as follows : CH : $C_c=0.36$ ($e_o+0.08LL-0.819$) CL : $C_c=0.269$ ($e_o+0.026LL-0.929$) 7. The cohesion of marine clays is no concerned with the increment of depth. The equations of relationship between cohesion and unconfined compression strength are as follows. CH : qu=1.896C+0.0107 CL : qu=1.849C+0.04.

  • PDF