• 한국융합학회논문지
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• 제9권8호
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• pp.101-108
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• 2018

본 연구는 상엽추출물이 포함된 보리면의 혈당조절능력을 입증하기 위하여 4주간 고지방/고탄수화물 식이 후 streptozotocin과 nicotinamide로 당뇨를 유발하고 음성대조군, 양성대조군, 보리면대조군, 상엽대조군, 보리면+상엽추출물 2.5%군, 보리면+상엽추출물 5%군의 6군으로 나누었다. 그 후 2주간 식이를 진행하며 체중, 혈당, 간 기능 및 혈액 내 지질의 변화를 측정하였다. 그 결과 보리면과 상엽 단독 섭취군보다 혼합섭취군에서 체중 감소가 적었고 혈당조절효과도 뛰어났으며 이는 경구 당 부하검사에서도 동일한 결과를 보였다. 또한 간기능, 혈중 지질 개선 효과도 혼합 섭취군에서 강하게 나타났다. 따라서, 보리면과 상엽추출물을 함유한 식이는 복합식이에서 단독식이보다 강한 혈당조절효과와 간 기능 개선, 지질 강하효과도 나타났다. 결과적으로 상엽을 포함하는 보리면은 혈당을 조절하고, 간 기능, 지질 강하 기능을 보여 만성질환 관리에도 도움이 될 것으로 사료된다.

• 생약학회지
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• 제41권4호
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• pp.282-288
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• 2010

We evaluated the anti-diabetic effects of Triticum aestivum sprout water extract (TA) in diabetes mellitus type 2. For the experiments, the diabetic animal model db/db mice were divided to 3 groups: diabetic control (db/db) and two experimental groups orally treated with 25 and 100 mg/kg single dose of TA (TA-25 and TA-100, respectively). The lean mice were used as the non-diabetic normal control. All mice have free access to water and AIN-93 diet. TA was administrated to diabetic mice for 5 weeks and the diabetic clinical markers, including blood glucose level, body weight, food intake and insulin level, were measured at a time. After administration for 5 weeks, the blood glucose level was decreased 1.10 and 1.98 folds in TA-25 and TA-100 groups, respectively, compared with db/db group. The body weight and diet consumption were significantly reduced by TA treatment in dose-dependent manner. The treatments of TA-100 also significantly decreased remarkedly liver weight and slightly serum insulin levels when compared with them of the diabetic control group. However the immunohistochemical staining for insulin clearly showed high expression of insulin in the pancreatic islet cells derived from all db/db mice, even if TA was administrated. Moreover, TA-100 treatment significantly improved impaired glucose tolerance in diabetic db/db mice. The results suggest that TA has anti-hyperglycemic effect attenuating blood glucose in the animal model of type 2 diabetes and might be useful as a functional diet for human diabetic diseases.

• 한국간호교육학회지
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• 제19권2호
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• pp.127-136
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• 2013

Purpose: The aim of the study was to evaluate the effect of self-monitoring of blood glucose (SMBG)-based Diabetes Self-Management Education (DSME) on glycemic control in type 2 diabetes. Methods: This study was designed to compare changes in glycemic control over 12months in SMBG-based DSME group (n=65) versus control group (n=65). Data were obtained from medical records type 2 diabetic patients treated with oral antidiabetic agents and above HbA1c 7.0% from June 2006 to August 2008. All participants completed DSME defined as informational intervention of lifestyle habits and reinforcement of educational Monthly News letter delivered by the diabetes nurse educator. SMBG-based DSME group requested to measure blood glucose 7 times a day for a week and to record their diary and received counseling with a focus on diet and lifestyle during the education. Assessments were conducted baseline, 3, 6 and 12 months. HbA1c was used as an index of glycemic control. Results: 12 months later, the level of HbA1c was reduced by $1.28{\pm}1.68%$ in experimental group and $0.49{\pm}1.05%$ in the control group. We found a significant effect of $Time^*$ Group interaction (p=.013). Conclusion: SMBG-based DSME for patients with type 2 diabetes with oral antidiabetic agents was effective in improving glycemic control and maintaining long-term glycemic control.

• Journal of Nutrition and Health
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• 제42권2호
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• pp.107-118
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• 2009

In patients with type 2 diabetes, oxidative stress could be increased by their metabolic changes. Elevated plasma homocysteine is considered as one of markers of enhanced oxidative stress. Due to oxidative stress, some complications like cardiovascular or renal diseases may develop in type 2 diabetes patients. Plasma homocysteine concentration may be increased if folate status were inadequate. Protective effects against oxidative stress may be diminished if the status of anti-oxidative nutrient as vitamin C was poor. It is, therefore, important to maintain adequate status of folate and vitamin C in type 2 diabetes patients. Thus, this study was performed to determine the effects of supplementation of folate and/or ascorbate on blood glycated hemoglobin ($HbA_{1c}$) level, serum concentrations of homocysteine and cholesterol, plasma oxidized low density-lipoprotein (LDL), concentration and plasma glutathione peroxidase (GSH-Px) activity in the patients with type 2 diabetes. A total of 92 type 2 diabetes patients participated voluntarily with written consents. They were divided into one of the four experimental groups; Control (C), Folate-supplemented (F), Ascorbate-supplemented (A), and Folate plus ascorbate-supplemented (FA). The subjects in C were taken placebo, those in F were supplemented 1 mg of folate, those in A received 1,000 mg of ascorbate, and those in FA were given 1 mg of folate plus 1,000 mg of ascorbate daily for 4 weeks. Supplementation of folate or ascorbate resulted to increase serum folate level or plasma ascorbate concentration apparently, respectively. Folate supplementation not ascorbate seemed to decrease plasma concentrations of homocysteine and oxidized LDL and reduce plasma GSH-Px activity. There might not be synergic effect of the supplementation of folate plus ascorbate. The results indicate that oxidative stress in the patients with type 2 diabetes may lower mainly by folate supplementation.

• 동의생리병리학회지
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• 제22권6호
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• pp.1537-1543
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• 2008

The purpose of this study was investigation to examine the prevention diabetic mellitus and treatment effect on Gastrodia Elata Blume(GEB) dieted at prior and after induced diabetic application. Prior induced diabetic 3 weeks ago application GEB dieted. It is to analysis changes in body weight, blood glucose, SOD, CAT and histopathological findings. For the fingding significantly concentration diabetic rats were divided into 3 different experimental groups and each groups were induced diabetic. Experimental group Ⅰ (STZ-induced diabetic rats; n=10), experimental group Ⅱ (after induced DM and GEB dieted rats; n=10), experimental group Ⅲ (Prior GEB dieted thereafter DM induced; n=10). Prior and After GEB dieted application was that body weight, blood glucose were increase in experimental group Ⅱ, Ⅲ. Specially, group Ⅲ was significantly change than group Ⅱ at 1st weeks. and the level of CAT were significantly decrease in experimental group Ⅱ, Ⅲ than group Ⅰ. but SOD level was increase in experimental group Ⅱ, Ⅲ than group Ⅰ. In histological observation; group Ⅰ showed decrease in the intensity and incidence of vacuolations, cellular infiltration and hypertrophy of in liver and kidney. The Gomori's stain result, group Ⅰ showed disruption ${\beta}$-cell in pancrease.

• Journal of Acupuncture Research
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• 제22권1호
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• pp.1-11
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• 2005

한약복합처방(SHP)의 약침 및 경구투여가 STZ으로 유발된 흰쥐의 당뇨병과 항산화능에 미치는 영향을 연구한 결과 다음과 같은 결론을 얻었다. 1. In vitro 에서 SHP은 ${\alpha}$-glucosidase 저해 및 DPPH 라디칼 소거 활성을 나타냈으며, t-BHP에 의한 신장 피질 조직에서의 지질 과산화 생성을 억제하였다. 2. SHP은 대조군에 비해 STZ에 의한 혈청 중 insulin 함량 저하를 유의성있게 증가시켰으며, 혈청 중 glucose 함량변화에 있어서도 유의성 있게 감소시켰다. 3. SHP은 대조군에 비해 STZ에 의해 상승된 혈청 중 triglyceride 함량을 유의성있게 감소시켰고, 혈청 중 total cholesterol 함량을 감소시키는 경향을 보였으나 유의성은 없었다. 4. STZ 투여로 인해 혈청 내 지질과산화물의 함량이 증가되었으며 SHP의 약침 및 경구투여로 감소되는 경향을 보였으나 유의성은 없었다. 5. STZ에 의해 증가된 catalase 활성은 SHP에 의해 감소되는 경향을 보였으나 유의성은 없었으며, glutathione 활성 역시 SHP에 의해 감소되는 경향을 보였으나 유의성은 없었다.

• 한국식품영양과학회지
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• 제41권6호
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• pp.759-765
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• 2012

본 연구에서는 5주간 야콘 추출물을 경구투여 하여 STZ에 의한 제1형 당뇨모델에서 주간혈당, 경구 당부하 검사, 랑게르한스섬의 크기 및 혈중 내 인슐린 농도를 관찰함으로써 당뇨 개선에 대한 평가를 하였다. 7주령 SD rats은 normal군, control군(STZ), GI(STZ+125 mg/kg 야콘), GII(STZ+250 mg/kg 야콘)와 GIII(STZ+500 mg/kg 야콘)으로 군 구성을 하였다. 주간 체중은 군간 차이가 없었으며, 주간 혈당은 5주차의 control군에 비하여 GIII군에서 감소하는 경향이 나타났으나 통계학적인 유의성은 없었다. 경구당부하 검사 결과는 STZ으로 유도된 동물모델에서 glucose 투여에 의한 혈당변화는 유의하게 감소하였고 maltose 투여에 의한 혈당변화는 감소하는 경향은 나타났으나 유의한 차이는 없었다. 또한 ICR계 mice에서의 경구 당부하 검사에서 maltose에 의한 혈당변화는 control군에 비하여 GIII군에서 유의하게 감소하였다. 더욱이 STZ에 의해 감소한 랑게르한스섬의 크기가 유의하게 증가되었으며, 비슷한 결과로 혈중 인슐린 농도가 증가되었다. 그러나 혈중 fructosamine, total cholesterol 및 triglyceride의 농도는 유의한 차이를 관찰할 수 없었다. 본 연구에서는 5주간의 야콘 추출물 섭취가 경구당부하 검사, 췌장조직 내 랑게르한스섬의 크기 및 혈중 인슐린 농도를 개선시킴으로써 당뇨 개선에 도움을 줄 것으로 생각된다.

• 대한한의학방제학회지
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• 제14권2호
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• pp.57-66
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• 2006

Objectives : The objective of this study is to observe the effect of Saryuktangkamibang, Saryuktang with Liriopis Tuber, Lycii Fructus, Schizandrae Fructus, and Bombyx Batryticatus, on the experimental diabetes. Methods : In order to induce diabetes experimentally, injected streptozotocin to the vein in the tail of rats and then treated oral administration of Saryuktangkamibang water extracts. In the measurement of the variation levels of glucose, ALP, creatinine, and BUN concentration for each concentration levels for serum (0.2g/100g, 0.4g/l00g, 0.6g/100g), concentration level of glucose significantly decreased in 0.4g/l00g concentration level of Saryuktangkamibang. With this 0.4g/l00g concentration level of Saryuktangkamibang, the following conclusion was derived from the measurement of the serum levels of glucose. ALP, AST, ALT, creatinine, and BUN concentration for the injection of each 4th, 11th, and 18th day. Results : 1. In the measurement for each concentrations, the glucose concentration level in the serum significantly decreased on the 9th day in the Saryuktangkamibang-dosed group. 2. The creatinine and BUN concentration level in the serum significantly decreased on the 9th day in the Saryuktangkamibang-dosed group to which 0.6g/100g injected. 3. In the measurement for each dosage dates, the glucose concentration level in the serum significantly decreased on the 11th day in the Saryuktangkamibang-dosed group to which 0.6g/100g injected. 4. The AST concentration level in the serum significantly decreased on the 11th day in the Saryuktangkamibang-dosed group. Conclusion : Saryuktangkamibang, Saruktang with Liriopis Tuber, Lycii Fructus, Schizandrae Fructus, and Bombyx Batryticatus, has an effect of hypoglycemia, with no hepato-renal dysfunction. For the experimental results above, in that nephrotoxicity significantly decreased on the 0.6g/100g concentration, another research such as study for effect in other ranges and study for effect of dosage term except the concentration range of this study is necessary. Also dosage of appropriate concentration of Saryuktangkamibang is thought to provide valuable aid to cure on diabetes.

• Clinical and Experimental Pediatrics
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• 제50권6호
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• pp.565-569
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• 2007

목 적 : 란투스는 최대효과 없이, 24시간 동안 효과가 지속되기 때문에 NPH에 비해 생리적인 기저 인슐린 대체 제제이다. 1형 당뇨병 소아 및 청소년에서 란투스와 휴마로그의 병합 치료와 혼합형 인슐린의 혈당 조절 효과를 비교하기 위해 본 연구를 시행 하였다. 방 법 : 혼합형 인슐린에서 란투스와 휴마로그의 병합 치료로 인슐린 요법을 바꾼 1형 당뇨병을 가진 20세 미만의 환아 25명을 대상으로 란투스 시작 전과 시작 6개월 후의 하루 인슐린 투여량, 월간 저혈당 횟수, 공복시 혈당, C-peptide 농도 및 당화혈색소를 비교하였다. 이들 중 11명의 환아와 혼합형 인슐린을 사용하는 10명의 환아를 대상으로 24시간 자가 혈당 검사를 시행하여 매 식사 30분 전과 식후 30분 간격으로 2시간 동안의 혈당과 취침 전의 혈당 비교하였다. 결 과 : 란투스 치료 6개월 후 저혈당 빈도가 월간 15.1회에서 7.6회로 50% 감소하였으며, 특히 야간 저혈당 빈도는 월간 6.7회에서 2.5회로 67% 감소하였다. 당화혈색소는 란투스 치료 6개월 후 9.3%에서 8.7%로 감소하였다. 24시간 혈당 검사에서는 란투스를 사용하는 군에서 아침 식후 30분, 60분, 90분, 120분에서의 혈당이 혼합형 인슐린을 사용하는 군보다 유의하게 낮았고, 24시간 평균 혈당은 란투스 사용군이 $164.1{\pm}78.2mg/dL$로 혼합형 인슐린 사용군의 $211.5{\pm}108.7mg/dL$보다 유의하게 낮았다. 결 론 : 1형 당뇨병 소아 및 청소년에서 란투스와 휴마로그의 병합 치료는 혼합형 인슐린과 비교하여 혈당 조절에 보다 효과적이고, 특히 야간 저혈당 감소에 유효한 것으로 생각된다.

• Journal of Microbiology and Biotechnology
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• 제17권12호
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• pp.2005-2017
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• 2007

Diabetic nephropathy remains a major cause of morbidity and mortality in the diabetic population and is the leading cause of end-stage renal failure. Despite current therapeutics including intensified glycemic control and blood pressure lowering agents, renal disease continues to progress relentlessly in diabetic patients, albeit at a lower rate. Since synthetic drugs for diabetes are known to have side effects, fungal mushrooms as a natural product come into preventing the development of diabetes. Our previous report showed the hypoglycemic effect of extracellular fungal polysaccharides (EPS) in streptozotocin (STZ)-induced diabetic rats. In this study, we analyzed the differential expression patterns of rat kidney proteins from normal, STZ-induced diabetic, and EPS-treated diabetic rats, to discover diabetes-associated proteins in rat kidney. The results of proteomic analysis revealed that up to 500 protein spots were visualized, of which 291 spots were differentially expressed in the three experimental groups. Eventually, 51 spots were statistically significant and were identified by peptide mass fingerprinting. Among the differentially expressed renal proteins, 10 were increased and 16 were decreased significantly in diabetic rat kidney. The levels of different proteins, altered after diabetes induction, were returned to approximately those of the healthy rats by EPS treatment. A histopathological examination showed that EPS administration restored the impaired kidney to almost normal architecture. The study of protein expression in the normal and diabetic kidney tissues enabled us to find several diabetic nephropathy-specific proteins, such as phospholipids scramblase 3 and tropomyosin 3, which have not been mentioned yet in connection with diabetes.