• Journal of Microbiology and Biotechnology
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• 제19권5호
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• pp.455-461
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• 2009

There is a significant amount of experimental evidence suggesting that polysaccharides from mushrooms enhance the host immune system by activating various mechanisms in immune cells, including macrophages. In this study, polysaccharides from Lentinus edodes were found to stimulate the functional activation of macrophages to secrete inflammatory mediators and cytokines and increase the phagocytotic uptake. The chemical properties of the stimulatory polysaccharides, CPFN-G-I, CPBN-G, and CPBA-G, were determined based on their monosaccharide composition, which mainly consisted of glucose and mannose. According to FT-IR and GC/MS, the structure of CPFN-G-I, purified from the fruiting body of L. edodes, was found to consist of a $\beta$-1,6-branched-$\beta$-1,4-glucan, whereas CPBN-G and CPBA-G, purified from the liquid culture broth, were found to be composed of a heteromannan. The configuration of the p-linkage and triple helical conformation of each polysaccharide were confirmed using a Fungi-Fluor kit and Congo red, respectively.

• 한국축산식품학회지
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• 제35권3호
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• pp.382-388
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• 2015

A duplex real-time polymerase chain reaction (PCR) based assay for the detection of porcine and horse meat in sausages was designed by using EvaGreen fluorescent dye. Primers were selected from mitochondrial 12S rRNA and 16S rRNA genes which are powerful regions for identification of horse and porcine meat. DNA from reference samples and industrial products was successfully extracted using the GIDAGEN® Multi-Fast DNA Isolation Kit. Genomes were identified based on their specific melting peaks (Mp) which are 82.5℃ and 78℃ for horse and porcine, respectively. The assay used in this study allowed the detection of as little as 0.0001% level of horse meat and 0.001% level of porcine meat in the experimental admixtures. These findings indicate that EvaGreen based duplex realtime PCR is a potentially sensitive, reliable, rapid and accurate assay for the detection of meat species adulterated with porcine and horse meats.

• 한방안이비인후피부과학회지
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• 제20권2호통권33호
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• pp.68-76
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• 2007

Objectives : This experimental study was performed to investigate the effects of Sulfur extract on anti-inflammation and anti-Propionibacterium acnes. Methods : The cytotoxicity of Sulfur extract about viability of Raw 264.7 cell were tested using a colorimetric tetrazolium assay(MTT assay). To investigate the anti-inflammation effects of Sulfur extract on LPS-induced macrophage Raw 264.7 cell, we used ELISA kit and Western blots. We evaluated anti-oxidation effects of Sulfur extract on HaCaT cell by Enzyme recycling method. And we investigated the inhibitory effects of Sulfur extract on Propionibactrium acnes using paper disk diffusion method. Results: 1. Sulfur extract has a little cytotoxicity in Raw 264.7 cell. 2. Concentration of $100\;{\mu}g/m{\ell}$ Sulfur extract inhibited the production of NO in the Raw 264.7 cell stimulated with LPS. 3. Sulfur extract showed a oxidation inhibition effect by decreasing the DPPH radicals. 4. Sulfur extract has not the significant inhibition effect of Propionibactrium acnes. Conclusions: These results indicate that Sulfur extract has anti-inflammation and anti-oxidation effects. If further study is performed, the use of Sulfur extract will be valuable and benificial in the therapy of Propionibactrium acnes.

• 한국소성가공학회:학술대회논문집
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• 한국소성가공학회 1998년도 춘계학술대회논문집
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• pp.155-159
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• 1998

This paper describes a research work of developing a computer-aided design and manufacturing (CAD-CAM) of irregular shaped sheet metal product for blanking or piercing and bending operation. An approach to the system is based on the knowledge-based rules. Knowledge for the system is formulated form plasticity theories, experimental results and the empirical knowledge of field experts. This system has been written in AutoLIST on the AutoCAD and in customer tool kit on the SmartCAM with a personal computer and is composed of nine modules. the system is designed by considering several factors, such as material and thickness of product, complexities of blank geometry and punch profile, diameter and material of a wire, and availability of press. This system is capable of unfolding a formed sheet metal to give flat pattern and automatically account for the adjustment of bending allowances to match tooling requirements by checking dimensions and generating NC data automatically according to drawings of die-layout module. Results carried out in each module will provide efficiencies to the designer and the manufacture of blanking or piercing and bending die in this field.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2007년도 Proceedings of ISRS 2007
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• pp.633-636
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• 2007

The objective of this study is to measure backscattering coefficients of paddy rice using L, C, X-bands scatterometer system during a rice growth period. The measurement was conducted at an experimental field located in National Institute of Agricultural Science and Technology (NIAST), Suwon, Korea. The rice cultivar was a kind of Japonica type, called Chuchung. The scatterometer system consists of dual-polarimetric square horn antennas, HP8720D vector network analyzer (20MHz ${\sim}$ 20GHz), RF cables, and a personal computer that controls frequency, polarization and data storage. The scatterometer system is calibrated using a calibration kit (3.5mm, 85052D). The backscattering coefficients were calculated by applying radar equation for the measured at incidence angles between $20^{\circ}$ and $60^{\circ}$ for four polarization (HR, VV, HV, VH), respectively, and compared with rice growth data such as plant height, stem number, biomass, dry weight and LAI that were collected at time of each scatterometer measurement simultaneously.

• 한국정밀공학회지
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• 제15권8호
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• pp.174-182
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• 1998

This paper describes a research work of developing a computer-aided design and machining of irregular shaped-sheet metal product for blanking or piercing operation. An approach to the CAD/CAM system is based on the knowledge-based rules. Knowledge for the CAD/CAM system is formulated from plasticity theories, experimental results and the empirical knowledge of field experts. The system has been written in AutoLISP on the AutoCAD and in customer tool kit on the SmartCAM with a personal computer and is composed of nine modules, which are input and shape treatment, flat pattern-layout, production feasibility check, blank-layout, strip-layout, die-layout, data conversion, modelling, and post-processor module. Based on knowledge-based rules, the system is designed by considering several factors, such as material and thickness of product, complexities of blank geometry and punch profile, diameter and material of a wire, and availability of press. This system is capable of generating NC data automatically according to drawings of die-layout module. Results which are carried out in each module will provide efficiencies to the designer and the manufacturer of blanking or piercing die in this field.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2006년도 춘계학술대회 논문집
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• pp.371-372
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• 2006

This study was carried out to analyze the effect of wind load on the structural stability of a container crane according to the change of the boom shape using wind tunnel test and provide a container crane designer with data which can be used in a wind resistance design of a container crane assuming that a wind load 75m/s wind velocity is applied in a container crane. Data acquisition conditions for this experiment were established in accordance with the similarity. The scale of a container crane dimension, wind velocity and time were chosen as 1/200, 1/13.3 and 1/15. And this experiment was implemented in an Eiffel type atmospheric boundary layer wind tunnel with $11.52m^2$ cross-section area. Each directional drag and overturning moment coefficients of a container crane according to the change of the boom shape were investigated.

• 대한한의학회지
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• 제23권1호
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• pp.133-144
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• 2002

Objectives : Korean traditional medicinal herbs have been used to improve the function of the hematopoietic system. The purpose of this study is to examine the hematopoietic effects of Juakium-derivative on aplastic anemia. Methods : In vitro, after bone marrow cells of mice with aplastic anemia induced by benzene were cultured with Juakium-derivative, we measured the gene expressions of hematopoietic cytokines, the intracellular TPO and SCF expression and the colony number of CFU-GEMM and BFU-E. Results : The results are summarized as follows : 1. The gene expressions of hematopoietic cytokines (TPO, c-mpl, SCF, c-kit, IL-3, EPO, EpoR, GM-CSF) and the TPO and SCF expression of the group treated with Juakium-derivative increased significantly more than those of the control group. 2. The colony number of CFU-GEMM and BFU-E in the rEPO plus rIL-3 plus Juakium-derivative group increased significantly compared with the only rEPO plus rIL-3 group. Conclusion : It was acknowledged that Juakium-derivative has hemato-potentiating effects on aplastic anemia induced by benzene and it is expected that Juakium-derivative can be used clinically for the patient with hematopoietic system disorder. This study could also be used further as important research data for Korean oriental medicine about hemato-potentiating. The exact mechanism explaining our study would need to be studied further.

• Clinical and Experimental Reproductive Medicine
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• 제3권1호
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• pp.13-19
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• 1976

Serum levels of human placental lactogen have been measured by hemagglutination-inhibition reaction in 67 normal pregnant state and in 15 postpartum 24 hour state, HAIR is less sensitive and reliable method than radioimmunoassay, but simple, rapid, less expensive and fairly accurate, so it is more helpful in screening of large antenatal population with or without high risk complications. 1) Sensitivity of HPL-HAIR test kit was $0.1{\mu}g$/ml of H.P.L. serum level and had no cross reaction to HCG or male serum or non-pregenant female or newborn infant, 2) H.P.L. value was around $2{\mu}g$/ml until 24th week of pregnancy and rose to $6{\sim}8$ ${\mu}g$/ml continuously until about 36th week of pregnancy and then slightly decreased or stationary. 3) H.P.L. value in postpartum 24 hour state was undetectable. 4) There was poor correlation between maternal serum H.P.L. value at term and baby weight.

• 한국통신학회논문지
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• 제39B권9호
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• pp.570-577
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• 2014

거리 기반 실시간 측위 시스템에서 보다 정확한 측위가 가능하기 위해서는 우수한 측위 알고리즘과 함께 거리측정의 정확도 확보도 필수적으로 요구된다. 본 논문에서는 두 노드 간의 실제적인 거리측정의 정확도를 평가하기 위해 기존의 Symmetry 가정이 제거된 SDS-TWR 거리계산식 유도에 의한 정성적인 분석을 수행하고, 개발된 센서노드를 활용해 테스트 네트워크를 구축한 후 실험결과에 의한 정량적인 평가를 동시에 수행한다. 본 연구에서 구현된 IEEE 802.15.4a 소프트웨어 스택을 탑재한 센서노드 환경이 기존 나노트론사의 상용 참조보드 환경보다 평균 약 60% 감소된 거리측정 오차율을 산출하여 상대적으로 매우 높은 거리측정 정확도를 보인다.