• Journal of Animal Science and Technology
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• 제59권7호
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• pp.16.1-16.6
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• 2017

Background: This study was performed to investigate the impact of exogenous ghrelin on the pancreatic ${\alpha}$-amylase outputs and responses of pancreatic proteins to ghrelin that may relate to pancreatic exocrine. Methods: Sprague-Dawley male rats (9 weeks old, $300{\pm}10g$) were injected with ghrelin via intraperitoneal (i.p.) infusion at dosage of 0, 0.1, 1.0 and $10.0{\mu}g/kg$ body weight (BW), respectively. The plasma ghrelin and cholecystokinin (CCK) level were determined using enzyme immunoassay kit; the mRNA expression of ghrelin receptor ($GHSR-1{\alpha}$) and growth hormone (GH) receptor were assessed by reverse transcription PCR; the expressions of pancreatic ${\alpha}$-amylase activity, extracellular-signal-regulated kinases (ERK), phosphorylated extracellular-signal-regulated kinases (pERK) and c-Jun N-terminal kinase (JNK) were evaluated by western blotting; moreover the responses of pancreatic proteins to ghrelin were analyzed using the two-dimensional gel electrophoresis system. Results: The exogenous ghrelin (1.0 and $10.0{\mu}g/kg\;BW$) elevated the level of plasma ghrelin (p < 0.05), and suppressed the expression of pancreatic ${\alpha}$-amylase at a dose of $10.0{\mu}g/kg\;BW$ (p < 0.05). No difference in the level of plasma CCK was observed, even though rats were exposed to any dose of exogenous ghrelin. In addition, a combination of western blot and proteomic analysis revealed exogenous ghrelin ($10.0{\mu}g/kg\;BW$) induced increasing the JNK and ERK expressions (p < 0.05) and four proteins such as Destrin, Anionic trypsin-1, Trypsinogen, and especially eukaryotic translation initiation factor 3 in rat pancreas. Conclusions: Taken together, exogenous ghrelin by i.p. infusion plays a role in the pancreatic exocrine secretion via mitogen-activated protein kinase signaling pathway.

• Clinical and Experimental Reproductive Medicine
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• 제18권2호
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• pp.143-151
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• 1991

An embryo-derived platelet activating factor (PAF) has been demonstrated to play an important role in reproduction. This report examined the effect of PAF on ovulation, fertilization, embryo development, implantation and fetal viability by using murine model. PAF had no stimulatory effect on ovulation and fertilization. But PAF had stimulatory effect on embryo development in in-vitro test, in spite of no effect on implantation and fetal viability. These results demonstrate that exogenous PAF could enhance embryo development and implantation and give suggestion that PAF may play an role in human IVF program.

• Asian-Australasian Journal of Animal Sciences
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• 제21권3호
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• pp.358-363
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• 2008

The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species such as pigs whose ooplasm is opaque and pronuclei are often invisible. In this study, the effects of sperm membrane disruption and electrical activation of oocytes on in vitro development and expression of transgene green fluorescent protein (GFP) in ICSI embryos were tested to refine this recently developed procedure. Prior to ICSI, sperm heads were treated with Triton X-100+NaCl or Triton X-100+NaCl+NaOH, to disrupt membrane to be permeable to exogenous DNA, and incubated with linearized pEGFP-N1 vector. To induce activation of oocytes, a single DC pulse of 1.3 kV/cm was applied to oocytes for $30{\mu}sec$. After ICSI was performed with the aid of a micromanipulator, in vitro development of embryos and GFP expression were monitored. The chemical treatment to disrupt sperm membrane did not affect the developmental competence of embryos. 40 to 60% of oocytes were cleaved after injection of sperm heads with disrupted membrane, whereas 48.6% (34/70) were cleaved without chemical treatment. Regardless of electrical stimulation to induce activation, oocytes were cleaved after ICSI, reflecting that, despite sperm membrane disruption, the perinuclear soluble sperm factor known to mediate oocyte activation remained intact. After development to the 4-cell stage, 11.8 (2/17, Triton X-100+NaCl+NaOH) to 58.8% (10/17, Triton X-100+NaCl) of embryos expressed GFP. The expression of GFP beyond the stage of embryonic genome activation (4-cell stage in the pig) indicates that the exogenous DNA might have been integrated into the porcine genome. When sperm heads were co-incubated with exogenous DNA following the treatment of Triton X-100+NaCl, GFP expression was observed in high percentage (58.8%) of embryos, suggesting that transgenic pigs may efficiently be produced using ICSI.

• Biomolecules & Therapeutics
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• 제13권3호
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• pp.174-180
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• 2005

Antigen presenting cells (APCs), dendritic cells (DCs) and macrophages, playa critical role not only in the initiation of immune responses, but also in the induction of immune tolerance. In an effort to regulate immune responses through the modulation of APC function, we searched for and characterized APC function modulators from natural products. Bifidobacterium pseudocatenulatum SPM1204 (SPM1204) isolated from feces of healthy Korean in the age of 20s was used in this experiment. DCs and macrophages were cultured in the presence of supernatants of SPM 1204 and then examined for their activities for the presentation exogenous antigen in association with major histocompatibility complexes (MHC) and macrophage activation. SPM1204 increased class I MHC-restricted presentation of exogenous antigen (cross-presentation) in a DC cell line, DC2.4 cells. The RAW 264.7 cell line was used to test the nonspecific effect of immune reinforcement of SPM1204 as a source of biological regulating modulator for the macrophage activation, include nitric oxide (NO) production and cytokine production. Results showed that the production of NO, tumor necrosis factor (TNF)-$\alpha$, interleukin 1 (IL-1)-$\beta$ and morphological changes in macrophages were largely affected by SPM1204 in a dose-dependent manner. Our results demonstrated that SPM1204 promote cross-presentation of dendritic cells as well as the induction of NO, TNF-$\alpha$ production, and activation of macrophage.

• 대한한의학원전학회지
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• 제27권4호
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• pp.15-20
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• 2014

Objectives : We search the contents about Bi disease in Huang Di Nei Jing(黃帝內經), to analyze the significance of Bi disease, etiopathogenisis and pathogenesis of Bi disease, treatment of Bi disease. Methods : We find that the key feature of Bi disease is joint pain induced by impatency of Qi and blood. Exterior and interior etiological factors are involved in, such as exogenous evil of cold and dampness, emotional disorders, intemperance of taking food, dysfunction of yingqi and weiqi, strong or weak constitution, etc. Results : The important pathogenesis are invaded by exogenous evil because of deficiency, disharmony of yingqi and weiqi and disharmony of five viscera. The key points of treatments are the individual concerned therapy and climate concerned therapy, selecting the acupoint according to the differentiation of symptoms and signs. Conclusions : The combined therapy should be used such as acupuncture and moxibustion, hot application of medicine, massage, Daoyin, outside apply, etc. These supply the theory foundation for etiological factor, pathogenesis, syndrome and treatment, and to direct the diagnosis and treatment of Bi disease later generations.

• 한국발생생물학회지:발생과생식
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• 제20권2호
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• pp.127-133
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• 2016

Purine metabolism is known factor for nuclear maturation of oocytes through both follicle cells and oocyte itself. However, it is largely unknown the roles of purine metabolism in the oocyte competence for fertilization and early development. In this study, the effects of adenosine in oocyte competence for development were examined using adenosine and its synthetic inhibitors. Adenosine treatment from GV intact stage for 18 hr (fGV) caused of decrease the fertilization rate but of increase the cleavage rate compared from the other stage treatment groups. Hadacidin did not effect on fertilization rate but increased cleavage rate without stage specificity. Adenosine did not block the effects of hadacidin with the exception of fGV group. Inhibition of purine synthetic pathways the fertilization rate was decreased in the fGV and fGVB groups but increased in the fMII group. Exogenous adenosine caused of decrease fertilization rate in the fGVB group but increase in the fMII group. Cleavage rate was dramatically increased in the adenosine treatment with synthetic inhibitors. It means that the metabolism of purine has stage specific effects on fertilization and cleavage. Exogenous adenosine had only can improve oocyte developmental competence when it treated at GV intact stage. On the other hand, endogenous synthesis in all maturation stage cause of increase the cleavage rate without effects on fertilization. These data suggest that adenosine at GV stage as a paracrine fashion and inhibitions of endogenous adenosine in all stage improve oocyte developmental competence.

• 한국조경학회지
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• 제23권4호
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• pp.50-60
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• 1996

The purpose of this study is to investigate the prime factors that affect user satisfaction of area and facilities in urban forests, to classify those factors, to find out factorial structure of user satisfaction about them, and then to suggest important indicators in the urban forest planning, design, and development. The survey research was done using the self-administered questionnaire method by the people in the city of the Pusan who participated in recreational activities in the Mt. Keumjung. Factor analysis was used to extract the prime factors related to the recreational satisfaction concerning the area and facilities of the urban forest by the SPSS program, and then prime factors were used to investigate the factorial structure of user recreational satisfaction in the urban forest by the LInear Structure RELation program. According to the factor analysis concerning the 13 variables, 4 prime factors by the statistical values were considered as effective, indicating, 'health and recreational facilities', 'convenience and management facilities','facilities for the physical and psychological training' and 'historical and cultural areas'. The results of the causal structure analysis were identified as having significant effects mutually on endogenous variables, 'overall recreational satisfaction of the areas and facilities in the Mt. Keumjung','health and recreational facilities','convenience and management facilities','facilities for the psychological training', and 'historical and cultural areas'. But exogenous variables which have significant effects on endogenous variables were also indentified. Overall fits of both causal model were very good. The hypothetical causal structural equation models based on the results found partially significant correlation between dndogenous variables and between envogenous and exogenous variables. These significant relationships indiate important factors and variables that should be considered in planning, design, and development of the urban forests. Therefore, development direction of the Mt. Keumjung has to be analyzed as significant factors concerning user recreational satisfaction in a systematic was, recognized as important variables for planning process. The plan for development and management also should reflect the natural conservation policy from the environmental conservation movement like natural conservation advertisement and environmental education.

• 한국조경학회지
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• 제20권1호
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• pp.29-38
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• 1992

The primary objective of this study is to investigate factors and variables which have significant effects on user satisfaction with recreational facilities in Children's Grand Park in Pusan City, theregby to establish the developmental way of urban park and open space. To test the causal models of this research, the data were gathered by self-administered questionnaires from 1085 households in Pusan City which were selected by the multi-stage probability sampling method. The analysis of the data primarily consists of two phase : The fist analysis dealt exploratory factor analysis which identified major factors involved in satisfaction with recreational facilities in Children's Grand Park and the second analysis tested the fit of the causal models of this research by employing LISREL methodology. The factor analysis identified that five factors are involved in satisfaction with recreational facilities. The five factors of satisfaction with recreational facilities are convenience and maintanance facilities, learnded recreational facilities, spaces for repose and relaxation, spaces for active recreation failities, and facilities for health and physical facilities. The second phase analysis tested the fit of the causal models for satisfaction with recreational facilities to the data and identified statistically significant causal linkage among overall satisfaction with Children's Grand Park, other endogenous factors and exogenous variables. Overall fits of both causal models were very good. Among endogenous factors, facilities for repose and relaxation, facilities for convenience and maintenance, learnded recreational facilities were identified as having significant effects on overall satisfaction. Exogenous variables which have significant effects on endogenous variables were also identified. These significant relationships indicate important factors and variables that should be considered in planning and development of urban park and open space. On the basis of these significant causal relationships, way for delovepment of urban park and open space were suggested.

• 대한교통학회지
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• 제29권3호
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• pp.93-101
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• 2011

우리나라의 교통사고 발생현황은 2008년 기준 215,822건으로 예년에 비하여 소폭 감소하는 추세이나 주요 선진국에 비해서는 여전히 높은 수준이다. 그 중 단속류 시설에서 발생하는 사고는 보행자들이 직접적으로 차량에 노출되어 차대사람 사고의 비율이 높아 심각한 결과를 유발하기 때문에 이를 방지하기 위한 추가적인 고려가 필요하다. 이에 교통사고의 유형별로 영향을 미치는 인자를 분석하였다. 단속류 시설에서 교통사고는 크게 차대차 사고와 차대 사람 사고로 그 성격과 특성이 구분될 수 있다. 따라서 교통사고 유형을 크게 두가지로 구분하고, 2005년부터 2007년까지 서대문구에서 발생한 교통사고 자료 분석을 통해 교통사고의 심각도와 외생적 변수들간의 관계를 추정하였다. 본 연구에서는 단속류 시설에서 교통사고 유형별 요인을 구조방정식모형(SEM : Structural Equation Modeling)을 이용하여 도출해내고, 모형을 구축하여 유형별로 사고의 주요인들을 파악하여 비교하였다. 최종 모형에서 도출된 결론은 차대차 사고에서는 도로 요인이, 차대사람 사고에서는 환경 요인이 크게 영향을 미치고 있는 것으로 분석되었다.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• 제26권6호
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• pp.565-580
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• 2000

This study was designed to evaluate the influence of cultured epidermal tissue graft and the administration of transforming growth factor(TGF)-${\beta}_3$ on maxillary growth in surgically created palatal defects. A total of 155 rats were divided into 2 groups according to surgical timing : postnatal 2 weeks(n=95), 4 weeks(n=40) and control(unoperated) group(n=20). The postnatal 2-week surgical group was subdivided into 3 groups according to repair methods: conventional surgery(Von Langenbeck technique)group(n=23); cultured tissue graft group(n=25); and full thickness skin graft group(n=25). Additionally, recombinant human TGF-${\beta}_3$ was administered(30ng or 150ng) on collagen matrix in surgically created palatal defects during surgery(9 conventional surgeries, 9 cultured tissue grafts) in 2-week-old rats. The results showed that all types of surgical treatment decreased maxillary growth compared with the control(unoperated) group(p<0.0001). On the other hand, the tissue graft group, whether cultured tissue or grafted skin, contributed to increased maxillary growth(p<0.0001).And exogenous TGF-${\beta}_3$ might play a role in connective tissue proliferation and new bone generation during wound healing on palatal defects. Our results suggest that grafting cultured epidermis with collagen matrix decreases the scar tension on maxillary growth more than conventional palatal surgery does. Therefore, exogenous TGF-${\beta}_3$ may contribute to accelerate wound healing on palatal defects.