Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
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MAXILLARY GROWTH FOLLOWING CULTURED EPIDERMAL TISSUE GRAFT AND THE ADMINISTRATION OF TGF-${\beta}_3$ ON SURGICALLY CREATED PALATAL DEFECTS IN RAT

백서 구개의 외과적 결손부에 자가배양상피조직 이식 및 TGF-${\beta}_3$ 투여가 상악골의 성장에 미치는 영향

  • Park, Jung-Hyun (Dept of Oral & Maxillofacial Surgery, College of Dentistry, Yonsei University) ;
  • Choi, Byung-Ho (Dept of Oral & Maxillofacial Surgery, College of Dentistry, Yonsei University) ;
  • Kang, Jung-Wan (Dept of Oral & Maxillofacial Surgery, College of Dentistry, Yonsei University) ;
  • Yook, Jong-In (Dept of Oral Pathology, College of Dentistry, Yonsei University) ;
  • Kim, Jin (Dept of Oral Pathology, College of Dentistry, Yonsei University) ;
  • Yi, Choong-Kook (Dept of Oral & Maxillofacial Surgery, College of Dentistry, Yonsei University)
  • 박정현 (연세대학교 치과대학 구강악안면외과학교실) ;
  • 최병호 (연세대학교 치과대학 구강악안면외과학교실) ;
  • 강정완 (연세대학교 치과대학 구강악안면외과학교실) ;
  • 육종인 (연세대학교 치과대학 구강병리학교실) ;
  • 김진 (연세대학교 치과대학 구강병리학교실) ;
  • 이충국 (연세대학교 치과대학 구강악안면외과학교실)
  • Published : 2000.12.31
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Abstract

This study was designed to evaluate the influence of cultured epidermal tissue graft and the administration of transforming growth factor(TGF)-${\beta}_3$ on maxillary growth in surgically created palatal defects. A total of 155 rats were divided into 2 groups according to surgical timing : postnatal 2 weeks(n=95), 4 weeks(n=40) and control(unoperated) group(n=20). The postnatal 2-week surgical group was subdivided into 3 groups according to repair methods: conventional surgery(Von Langenbeck technique)group(n=23); cultured tissue graft group(n=25); and full thickness skin graft group(n=25). Additionally, recombinant human TGF-${\beta}_3$ was administered(30ng or 150ng) on collagen matrix in surgically created palatal defects during surgery(9 conventional surgeries, 9 cultured tissue grafts) in 2-week-old rats. The results showed that all types of surgical treatment decreased maxillary growth compared with the control(unoperated) group(p<0.0001). On the other hand, the tissue graft group, whether cultured tissue or grafted skin, contributed to increased maxillary growth(p<0.0001).And exogenous TGF-${\beta}_3$ might play a role in connective tissue proliferation and new bone generation during wound healing on palatal defects. Our results suggest that grafting cultured epidermis with collagen matrix decreases the scar tension on maxillary growth more than conventional palatal surgery does. Therefore, exogenous TGF-${\beta}_3$ may contribute to accelerate wound healing on palatal defects.

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