• The KIPS Transactions:PartC
• /
• v.17C no.5
• /
• pp.407-414
• /
• 2010

A condition access system (CAS) refers to a hardware-based system that allows only authenticated users to have access to contents. The CAS has many disadvantages found in that in the replacement of multiple service operator (MSO) a set-top box should be also changed and the smart-card often causes malfunction. To deal with the problems, exchangeable CAS (XCAS) was developed in 2009. However the standards or evaluation schemes for XCAS are absent. Existing evaluation schemes are not appropriate for evaluating XCAS due to the evaluation standard, the evaluation cost and efficiencies. Therefore, a specific scheme that can evaluate the security and suitability of exchangeable conditional access systems has been requested. In this paper, we propose an appropriate evaluation scheme for XCAS. The evaluation scheme includes an evaluation purpose and four components to evaluate the evaluation target, the evaluation process, the evaluation subject, and the evaluation cost involved.

• The KIPS Transactions:PartC
• /
• v.17C no.4
• /
• pp.347-360
• /
• 2010

A condition access system (CAS) refers to a hardware-based system that allows only authenticated users to have access to contents. The CAS has many disadvantages found in that in the replacement of multiple service operator (MSO) a set-top box should be also changed and the smart-card often causes malfunction. To deal with the problems, exchangeable CAS (XCAS) was developed in 2009. However, in the XCAS, no method to authenticate a proper set-top box has been put forward. In this paper, we propose a novel program for set-top authentication in the XCAS. Additionally, we offer a format of certificate of authentication, and procedures of issuing the certificate for broadcasting services suitable for the XCAS. The technical method of authentication a set-top box that will be discussed is of high efficiency since in the MSO it requires only two subjects to communicate during the authentication in the MSO.

• Molecules and Cells
• /
• v.42 no.6
• /
• pp.460-469
• /
• 2019

Bacterial ${\alpha}-type$ carbonic anhydrase (${\alpha}-CA$) is a zinc metalloenzyme that catalyzes the reversible and extremely rapid interconversion of carbon dioxide to bicarbonate. In this study, we report the first crystal structure of a hyperthermostable ${\alpha}-CA$ from Persephonella marina EX-H1 (pmCA) in the absence and presence of competitive inhibitor, acetazolamide. The structure reveals a compactly folded pmCA homodimer in which each monomer consists of a 10-stranded ${\beta}-sheet$ in the center. The catalytic zinc ion is coordinated by three highly conserved histidine residues with an exchangeable fourth ligand (a water molecule, a bicarbonate anion, or the sulfonamide group of acetazolamide). Together with an intramolecular disulfide bond, extensive interfacial networks of hydrogen bonds, ionic and hydrophobic interactions stabilize the dimeric structure and are likely responsible for the high thermal stability. We also identified novel binding sites for calcium ions at the crystallographic interface, which serve as molecular glue linking negatively charged and otherwise repulsive surfaces. Furthermore, this large negatively charged patch appears to further increase the thermostability at alkaline pH range via favorable charge-charge interactions between pmCA and solvent molecules. These findings may assist development of novel ${\alpha}-CAs$ with improved thermal and/or alkaline stability for applications such as $CO_2$ capture and sequestration.

• Korean Journal of Environmental Agriculture
• /
• v.35 no.4
• /
• pp.247-255
• /
• 2016

BACKGROUND: Acrylamide (CAS No. 79-06-1) is known to be a carcinogenic compound, and is classified as a Group 2A compound by the International Agency for Research on Cancer (IARC, 1994). Acrylamide can be generated during the browning process via the non-enzymatic Maillard reaction of carbohydrates such as reducing sugars and of amino acids such as asparagine, both of which occur at a temperature above $120^{\circ}C$. Potato tubers contain reducing sugars, and thus, this will affect the safety of processed potato products such as potato chips and French fries. In order to reduce the level of acrylamide in potato processed products, it is therefore necessary to understand factors that affect the reducing sugar content of potatoes, such as environmental factors and potato storage conditions, as well as understanding factors affecting acrylamide formation during potato processing itself. METHODS AND RESULTS: Potatoes were cultivated in eight regions of Korea; For each of these different environments, soil physico-chemical characteristics such as pH, electrical conductivity, total nitrogen, available phosphate, and exchangeable cation content were measured and correlations with potato reducing sugar content and potato chip acrylamide levels were examined. The reducing sugar content in potato during storage for three months was determined and acrylamide level in potato chip was analyzed after processing. The storage temperature levels were $4^{\circ}C$, $8^{\circ}C$, or $10^{\circ}C$, respectively. The acrylamide content of chips prepared from potatoes stored at $10^{\circ}C$ or $20^{\circ}C$ for one month was analyzed and the different frying times were 2, 3, 5, and 7 min. CONCLUSION: This study showed that monitoring and controlling the phosphate content within a potato field should be sufficient to avoid producing brown or black potato chips. For potatoes stored at low temperatures, a reconditioning period ($20^{\circ}C$ for 20 days) is required in order to reduce the levels of reducing sugars in the potato and subsequently reduce the acrylamide and improve chip coloration and appearance.