• The Journal of Engineering Geology
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• v.11 no.1
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• pp.1-11
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• 2001

The geological characteristics of Korea are that we can encounter the rock layer only after 10m of excavation, methods to presume the rock pressure distribution of the rock layer is urgently needed. When using the existing empiric science of Terzaghi-Peck, Tschebotarioff to measure the rock pressure of the rock layer, underestimate the real strength because of the cohesion is ignored. Therefore calculating the horizontal sliding force of wedge block, which includes the dips and shear strength of discontinuities and surcharge load etc., think to be to getting a closer rock stress of the real rock pressure acting upon the earth structure in rock mass. This research use Coulomb soil pressure theory assuming that the backfill soil will yield wedge failure when it has cohesion, applying Prakash-Saran(l963), and then it uses equilibrium of force and shear strength $\tau$=c+$\sigma$tan $\Phi$ of the cliscontinuities. Analyzing shear strength and dips of cliscontinuities using calculated theory according to the status of discontinuities aperture, we were able to find out that because the cohesion and friction angle of the rock layer itself is large enough, how the dip directions and dips facing the excavation face is the only factor deciding whether or not the rock stress is applied. The evaluated theory of this research should be strictly estimated, so that the many parameters such as c, $\Phi$value, types and structures of rock class, excessive lateral pressure, dynamic load, earthquake, needed later when calculating shear strength of discontinuities and especially the ground water effect acting on rock layer should be coumpted with many measuring data achieve at the insite to study the application.

• Proceedings of the Korean Society for Rock Mechanics Conference
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• 2000.09a
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• pp.93-103
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• 2000

The concrete plug in an underground cavern prevents the stored product (oil, gas, etc) from leaking and the excessive show of underground water, so it plays an important role in construction and operation of the storage cavern. Additionally, it should maintain its stability under every possible loading condition. Once the plug is constructed, the cavern is isolated from the external access. Therefore, mechanical and hydraulic consideration should be made in construction to fulfill its function. Therefore, in this study, numerical analyses were conducted to study the optimal shape and thickness of the plug with respect to the various conditions of installation depth, the shape of the plug, in-situ stress ratio (K), the condition of rock-plug interface, and the effect of Excavation Damaged Zone (EDZ). This paper also presents the effect of slot depth on the hydraulic behavior of the plug. These analyses were carried out by using the 2-dimensional finite difference code, rm FLAC, and the 3D code, m FLA $C^{3D}$./.

• Tunnel and Underground Space
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• v.10 no.3
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• pp.344-354
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• 2000

The concrete plug in an underground cavern prevents the stored product (oil, gas, etc) from leaking and the excessive inflow of underground water, so it plays an important role in construction and operation of the storage cavern. Additionally, it should maintain its stability under every possible loading condition. Once the plug is constructed, the cavern is isolated from the external access. Therefore, mechanical and hydraulic consideration should be made in construction to fulfill its function. Therefore, in this study, numerical analyses were conducted to study the optimal shape and thickness of the plug with respect to the various conditions of installation depth, the shape of the plug, in-situ stress ratio (K), the condition of rock-plug interface, and the effect of Excavation Damaged Zone (EDZ). This paper also presents the effect of slot depth on the hydraulic behavior of the plug. These analyses were carried out by using the 2-dimensional finite difference code, rm FLAC, and the 3D code, rm FLAC$\^$3D/.

• Toxicological Research
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• v.31 no.1
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• pp.17-23
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• 2015

Excessive manganese (Mn) in the brain promotes a variety of abnormal behaviors, including memory deficits, decreased motor skills and psychotic behavior resembling Parkinson's disease. Hereditary hemochromatosis (HH) is a prevalent genetic iron overload disorder worldwide. Dysfunction in HFE gene is the major cause of HH. Our previous study has demonstrated that olfactory Mn uptake is altered by HFE deficiency, suggesting that loss of HFE function could alter manganese-associated neurotoxicity. To test this hypothesis, Hfe-knockout ($Hfe^{-/-}$) and wild-type ($Hfe^{+/+}$) mice were intranasally-instilled with manganese chloride ($MnCl_2$ 5 mg/kg) or water daily for 3 weeks and examined for memory function. Olfactory Mn diminished both short-term recognition and spatial memory in $Hfe^{+/+}$ mice, as examined by novel object recognition task and Barnes maze test, respectively. Interestingly, $Hfe^{-/-}$ mice did not show impaired recognition memory caused by Mn exposure, suggesting a potential protective effect of Hfe deficiency against Mn-induced memory deficits. Since many of the neurotoxic effects of manganese are thought to result from increased oxidative stress, we quantified activities of anti-oxidant enzymes in the prefrontal cortex (PFC). Mn instillation decreased superoxide dismutase 1 (SOD1) activity in $Hfe^{+/+}$ mice, but not in $Hfe^{-/-}$ mice. In addition, Hfe deficiency up-regulated SOD1 and glutathione peroxidase activities. These results suggest a beneficial role of Hfe deficiency in attenuating Mn-induced oxidative stress in the PFC. Furthermore, Mn exposure reduced nicotinic acetylcholine receptor levels in the PFC, indicating that blunted acetylcholine signaling could contribute to impaired memory associated with intranasal manganese. Together, our model suggests that disrupted cholinergic system in the brain is involved in airborne Mn-induced memory deficits and loss of HFE function could in part prevent memory loss via a potential up-regulation of anti-oxidant enzymes in the PFC.

• Herbal Formula Science
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• v.23 no.2
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• pp.189-198
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• 2015

Objectives : Sparganii Rhizoma is frequently used in traditional herbal medicine for treatment of blood stasis, amenorrhea and functional dyspepsia and has been reported to exhibit anti-oxidant, anti-proliferation and anti-angiogenesis peoperties. In this study, we investigated the cytoprotective effect and underlying mechanism of Sparganii Rhizoma water extract (SRE) against oxidative stress-induced mitochondrial dysfunction and apoptosis in hepatocyte. Methods : To determine the effects of SRE on oxidative stress, we induced synergistic cytotoxicity by co-treatment of arachidonic acid (AA) and iron in the HepG2 cell, a human derived hepatocyte cell line. Results : Treatment of SRE increased relative cell viability and altered the expression levels of apoptosis-related proteins such as Bcl-xL, Bcl-2 and procaspase-3. And SRE also inhibited the mitochondrial dysfunction and excessive reactive oxygen species production induced by AA+iron. In addition, SRE activated of AMP-activated protein kinase (AMPK), a potential target for cytoprotection, by increasing the phosphorylation of AMPKα at Thr-172. Morever, SRE increased phosphorylation of acetyl-CoA carboxylase, a direct downstream target of AMPK. Conclusion : These results indicated that SRE has the ability to protect against oxidative stress-induced hepatocyte damage, which may be mediated with AMPK pathway.

• Journal of Electrochemical Science and Technology
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• v.14 no.4
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• pp.388-396
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• 2023

Photoelectrochemical (PEC) water splitting is a vital source of clean and sustainable hydrogen energy. Moreover, the large-scale H₂ production is currently necessary, while long-term stability and high PEC activity still remain important issues. In this study, a GaN-based photoelectrode was modified by an additional NH₃ treatment (900℃ for 10 min) and its PEC behavior was monitored. The bare GaN exhibited a highly crystalline wurtzite structure with the (002) plane and the optical bandgap was approximately 3.2 eV. In comparison, the NH₃-treated GaN film exhibited slightly reduced crystallinity and a small improvement in light absorption, resulting from the lattice stress or cracks induced by the excessive N supply. The minor surface nanotexturing created more surface area, providing electroactive reacting sites. From the surface XPS analysis, the formation of an N-Ga-O phase on the surface region of the GaN film was confirmed, which suppressed the charge recombination process and the positive shift of E_FB. Therefore, these effects boosted the PEC activity of the NH₃-treated GaN film, with J values of approximately 0.35 and 0.78 mA·cm^-2 at 0.0 and 1.23 V_RHE, respectively, and an onset potential (V_on) of -0.24 V_RHE. In addition, there was an approximate 50% improvement in the J value within the highly applied potential region with a positive shift of V_on. This result could be explained by the increased nanotexturing on the surface structure, the newly formed defect/trap states correlated to the positive V_on shift, and the formation of a GaO_xN_1-x phase, which partially blocked the charge recombination reaction.

• Journal of Dental Anesthesia and Pain Medicine
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• v.17 no.1
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• pp.21-28
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• 2017

Background: The skin consists of tightly connected keratinocytes, and prevents extensive water loss while simultaneously protecting against the entry of microbial pathogens. Excessive cellular levels of reactive oxygen species can induce cell apoptosis and also damage skin integrity. Propofol (2,6-diisopropylphenol) has antioxidant properties. In this study, we investigated how propofol influences intracellular autophagy and apoptotic cell death induced by oxidative stress in human keratinocytes. Method: The following groups were used for experimentation: control, cells were incubated under normoxia (5% $CO_2$, 21% $O_2$, and 74% $N_2$) without propofol; hydrogen peroxide ($H_2O_2$), cells were exposed to $H_2O_2$ ($300{\mu}M$) for 2 h; propofol preconditioning (PPC)/$H_2O_2$, cells pretreated with propofol ($100{\mu}M$) for 2 h were exposed to $H_2O_2$; and 3-methyladenine $(3-MA)/PPC/H_2O_2$, cells pretreated with 3-MA (1 mM) for 1 h and propofol were exposed to $H_2O_2$. Cell viability, apoptosis, and migration capability were evaluated. Relation to autophagy was detected by western blot analysis. Results: Cell viability decreased significantly in the $H_2O_2$ group compared to that in the control group and was improved by propofol preconditioning. Propofol preconditioning effectively decreased $H_2O_2$-induced cell apoptosis and increased cell migration. However, pretreatment with 3-MA inhibited the protective effect of propofol on cell apoptosis. Autophagy was activated in the $PPC/H_2O_2$ group compared to that in the $H_2O_2$ group as demonstrated by western blot analysis and autophagosome staining. Conclusion: The results suggest that propofol preconditioning induces an endogenous cellular protective effect in human keratinocytes against oxidative stress through the activation of signaling pathways related to autophagy.

• Journal of Plant Biotechnology
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• v.44 no.3
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• pp.320-324
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• 2017

Development of modern agricultural machinery and accompanying agricultural development cause soil compaction and reduce growth by stressing roots. Kalanchoe pinnata was used to investigate the impact of stress on rooting and changes in plant growth and reproduction. K. pinnata forms somatic embryos capable of asexual reproduction at the edge of leaves. Impact of root pressurization of K. pinnata on somatic embryogenesis and organ differentiation according to external stress factors was investigated by using a high concentration of agar and this phenomenon was studied histologically. Agar concentration in culture media ranged from 0.5%-1.5% to induce a compression effect on roots. The stem and leaf of K. pinnata were subjected to a microtechnique process to study changes in tissue. In vivo, K. pinnata produced 2nd and 3rd plantlets at edges of leaves from lack of water and excessive lighting conditions. In in vitro culture studies, the lower the concentration of agar, the higher the population and the higher the biomass, but plantlet did not occur in leaf bends. Conversely, as concentration of agar increased, increase in the number of individuals was low. Plantlet development occurred only in agar 1.5% medium. The difference in agar concentration was a stressor in the root of K. pinnata, and thus the pattern of asexual reproduction changed from the division method in root to a plantlet generation in leaf. This suggests root pressurization may act as stress and change in the plant reproduction pattern.

• Journal of the Korea institute for structural maintenance and inspection
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• v.27 no.4
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• pp.94-103
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• 2023

Built in urban ares tunnels is necessary to accurately grasp not only the above-ground environment of the tunnel but also the below-ground environment of the tunnel for design and construct. However, fully coupled analysis of stress and flow is very difficult due to the limited function of the tunnel numerical analysis program and difficulty in using program. This can lead to excessive design that increases the construction cost or occur problems that can lead to accidents during construction. In particular, in the case of an urban tunnel has a low layer soil section above the tunnel and the groundwater level exists in the upper layer of the tunnel. Therefore, a reduction in the groundwater level during underground construction may increase the effective stress of the upper layer and cause the ground to subsidence. So It is necessary to design after accurately evaluating the change in the groundwater level. In this study, the tunnel's behavioral characteristics were analyzed through fully coupled analysis of stress and flow according to the drainage type for an urban underground tunnel.

• Journal of Applied Biological Chemistry
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• v.64 no.2
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• pp.165-170
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• 2021

In this study, the antioxidant effects of mineral-containing deep sea water (DSW) on kidney function was confirmed using a cell model. DSW samples were prepared with different mineral concentrations including calcium and magnesium-the main minerals found in DSW-to derive the following sample groups: trace minerals (TM), high magnesium (HM), high magnesium, low salt (HMLS) and high magnesium, high calcium (HMHC). The purpose of this preparation was to determine the optimal calcium/magnesium ratio in DSW. Human embryonic kidney (HEK293) cells were exposed to sodium chloride (NaCl) for 2 h to induce release of reactive oxygen species (ROS). Thereafter, the cells were treated with the respective DSW samples before ROS concentrations, as well as antioxidant enzyme activity and protein levels, were measured. Among the water samples, HMLS showed the most protective effect against ROS, whereas the intracellular glutathione content was highest in cells from the HMLS- and HMHC-treated groups. However, TM- and HMHC-treated cells showed similar tendencies to the control group, in terms of mRNA expression of antioxidant genes. These results suggested that DSW may aid in preventing renal oxidative stress caused by excessive sodium intake. Furthermore, it was determined that HMLS and HMHC water samples displayed good antioxidant effects in the kidney cell model, based on the combined results of ROS concentration and antioxidant marker measurements.