• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.7
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• pp.597-606
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• 2016

This is descriptive research to understand the constipation rate of elementary school students due to their differences in cognition of their eating habits, hours of exercise/physical activity, stress, and school restroom environment in accordance with the matter of constipation, as well as the factors related to the constipation rate. A total of 741 students (grade3-6) from six elementary schools in Jeollanam-do were targeted; the survey was conducted from April 30th to May 12th, 2014. The frequency, percentage, ${\chi}^2$ test, independent t-test, and bivariate logistic regression analysis were conducted. The constipation rate was 23.9%, and there were factors related to the constipation rate, such as restroom preference, matter of defecation during breaks, number of meals per day, number of breakfasts skipped, size of the meal, hours of institute class, hours of internet use, stress, and cognition of school restroom environment. In particular, students with 'high' stress had a 5.44 times higher rate of constipation than students with 'low' stress. As subjective symptoms are limited in diagnosing constipation, a detailed medical examination and physical checkup should be included in the process of understanding the constipation rate, as well as in education and management to prevent elementary school students from having constipation.

• The Korean Journal of Physiology and Pharmacology
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• v.4 no.3
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• pp.219-226
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• 2000

In order to elucidate the role of platelet activating factor (PAF) in the acute lung injury induced by endotoxin (ETX), activities of phospholipase A2, lyso PAF acetyltransferase and oxidative stress by neutrophilic respiratory burst were probed in the present study. To induce acute lung injury, $100\;{\mu}g$ of E.coli ETX (type 0127; B8) was instilled directly into the tracheae of Sprague-Dawley rats. Five hours after the ETX instillation, induction of acute lung injury was confirmed by lung leak index and protein contents in the bronchoalveolar lavage (BAL) fluid. At the same time, lung phospholipase A2 (PLA2) activity and expression of group I and II secretory type PLA2 were examined. In these acutely injured rats, ketotifen fumarate, known as lyso PAF acetyltransferase inhibitor and mepacrine were administered to examine the role of PAF in the pathogenesis of the acute lung injury. To know the effect of the ETX in the synthesis of the PAF in the lungs, lyso PAF acetyltransferase activity and PAF content in the lungs were measured after treatments of ETX, ketotifen fumarate and mepacrine. In addition, the role of neutrophils causing the oxidative stress after ETX was examined by measuring lung myeloperoxidase (MPO) and enumerating neutrophils in the BAL fluid. To confirm the oxidative stress in the lungs, pulmonary contents of malondialdehyde (MDA) were measured. After instillation of the ETX in the lungs, lung leak index increased dramatically (p＜0.001), whereas mepacrine and ketotifen decreased the lung leak index significantly (p＜0.001). Lung PLA2 activity also increased (p＜0.001) after ETX treatment compared with control, which was reversed by mepacrine and ketotifen (p＜0.001). In the examination of expression of group I and II secretory PLA2, mRNA synthesis of the group II PLA2 was enhanced by ETX treatment, whereas ketotifen and WEB 2086, the PAF receptor antagonist, decreased the expression. The activity of the lysoPAF acetyltransferase increased (p＜0.001) after treatment of ETX, which implies the increased synthesis of PAF by the remodelling of lysoPAF in the lungs. Consequently, the contents of the PAF in the lungs were increased by ETX compared with control (p＜0.001), while mepacrine (p＜0.001) and ketotifen (p＜0.01) decreased the synthesis of the PAF in the lungs of ETX treated rats. The infiltration of the neutrophils was confirmed by measuring and enumerating lung MPO and the neutrophils in the BAL fluid respectively. Compared with control, ETX increased lung MPO and number of neutrophils in BAL significantly (p＜0.001) whereas mepacrine and ketotifen decrerased number of neutrophils (p＜0.001) and MPO (p＜0.05, p＜0.001, respectively). The lung MDA contents were also increased (p＜0.001) by ETX treatment, but treatment with mepacrine (p＜0.001) and ketotifen (p＜0.01) decreased the lung MDA contents. Collectively, we conclude that ETX increases PLA2 activity, and that the subsequently increased production of PAF was ensued by the remodelling of the lyso PAF resulting in tissue injury by means of oxidative stress in the lungs.

• Journal of Mushroom
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• v.16 no.3
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• pp.213-217
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• 2018

This study was carried out to identify medicinal mushrooms with protective effects against oxidative stress in PC12 neuronal cell line, followed by evaluation of their antioxidant property. Extracts of medicinal mushrooms, including Ganoderma lucidum extract (GLE), antler-shaped Ganoderma lingzhi extract (AGLE), Hericium erinaceus extract (HEE), and Sanghuangporus baumii extract (SBE), were screened for cytotoxicity using MTT assay. None of the extracts up to $10{\mu}g/ml$ concentration affected cell viability. These extracts were further checked for their protective effect against oxidative stress-induced reactive oxygen species (ROS) production. Exposure to $50{\mu}M$ $H_2O_2$ induced ROS generation in PC12 cells, which was inhibited only by treatment with AGLE. In addition, inhibition of $H_2O_2-induced$ ROS generation by AGLE was found to be in a dose-dependent manner (2.5, 5, and $10{\mu}g/ml$). Microscopic examination of DCF fluorescence for detection of ROS showed a similar pattern. Further, antioxidant activity of AGLE was determined by ABTS radical cation assay, and its $IC_{50}$ was found to be $46.90{\pm}0.31{\mu}g/ml$. Taken together, these results suggest that AGLE may help to alleviate oxidative stress in PC12 neuronal cells.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.17 no.8
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• pp.369-378
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• 2016

The purpose of this study was to determine the effects of mother's education about amblyopia occlusion therapy on parenting stress and efficacy and the visual acuity improvement of the amblyopia child. The study was designed as a nonequivalent control group pretest posttest quasi-experimental study. The mothers with amblyopia children who signed the consent form were assigned to one of two groups, the experimental group (29) and control group (31). The data were collected from March 5 to October 5, 2010, at G university hospital outpatient department located in G city. The subjects in the experimental group participated in the Mothers Education about Amblyopia Occlusion Therapy for 24 weeks, which included face to face consultations, telephone counseling, etc.. The degree of parenting stress and efficacy were measured by using questionnaires and the visual acuity was measured by visual examination. The data were analyzed using the ${\chi}^2-test$, Fisher's exact probability test, t-test and Repeated Measure ANOVA using SPSS PC+20.0 for Windows. The following results were found after the therapy. The experimental group had statistically significantly higher visual acuity (Rt, F=40.64, p<.001, Lt, F=10.28, p<.001), lower parenting stress scores (F=9.85, p=.003) and higher parenting efficacy scores (F=22.19, p<.001) than the control group. In conclusion, the Mothers about Amblyopia. Occlusion Therapy Education Program was effective in improving the visual acuity of the amblyopia children, and decreasing the parenting stress and enhancing the parenting efficacy of their parents.

• Fisheries and Aquatic Sciences
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• v.23 no.9
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• pp.26.1-26.9
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• 2020

Background: The study evaluated the effects of a butaphosphan and cyanocobalamin mixture on the immune system and stress in olive flounders, Paralichthys olivaceus. Methods: The mixture was intramuscularly injected into olive flounders at the current recommended dose. Furthermore, to determine the toxicity of overdose, a histological examination was performed after injection of 1-, 2-, and 4-fold higher than the recommended dose. Results: Immunity parameters were altered during the first 2 weeks after a single intramuscular injection of the mixture in olive flounders (average weight 20.5 ± 1.1 g). The levels of all tested items, except glutathione and antiprotease, were higher in the treated group than in the control group in the first week; the levels of all tested items were even higher in the second week in the treated group than in the control group. The level of nitro-blue tetrazolium, myeloperoxidase, and superoxide dismutase between the two groups differed significantly. Changes in the stress response to different seawater temperatures (increase or decrease in seawater temperature by 3-5 ℃ using 50 L heated or cooled seawater tanks) were studied by determining the changes in cortisol and glucose levels on days 1 and 7. Both cortisol and glucose levels were significantly lower in the treated group than in the control group. Histological analysis did not reveal any abnormalities after intramuscular injection of the mixture at doses that were 1-, 2-, and 4-fold higher than the recommended dose. Conclusions: Intramuscular injection of a butaphosphan and cyanocobalamin mixture is safe and effective in reducing stress and improving immunity in olive flounders.

• Journal of Society of Preventive Korean Medicine
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• v.8 no.1
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• pp.47-58
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• 2004

This study was conducted to evaluated the degree of job-related life style and stress of industrial workers to present fundamental materials of preventive oriental medicine. The medical examination with oriental medicine method was performed from October 14 to November 1, 2002 for the 474 industrial workers and general characters, life style and Psychosocial Well-being Index(PWI) were collected by using self-recording type questionnaire. The results were as follows; 1) All 474 of subjects were male, job type was distributed into technician, 37.8% of them, white collar worker, blue collar worker, research worker in an orderly fashion. 2) As a result of comparison between the job types in general characters, research worker group was the highest in education level, above 10 years group in employee duration and 1.5-2.99 million won group in monthly income was the largest group in all job type and the distribution was significantly different. 3) Analyzing the difference in life style according to the job type shows that significantly highest prevalence of drinking alcohol was found in blue collar workers, they drank alcohol 2-4 times a week and that workers did not smoke in all group of job type and the prevalence of exercise was significantly high in less than 1 time a week. 4) Analyzing the difference in the degree of stress according to the job type shows that blue collar worker group had the highest score in Factor 1, Factor 4 fields, technician group had the highest score in Factor 2 field and the differences were significant. Considering above results, the present study shows that there are difference in life style and stress according to the job type in industrial workers and that health management according to job type must be required to improve health condition and to prevent disease of industrial workers in the future.

• Animal Bioscience
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• v.34 no.10
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• pp.1590-1599
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• 2021

Objective: This study investigates the expression patterns of toll-like receptors (TLRs) and intracellular mediators in horse muscle cells after exercise, and the relationship between TLRS expression in stressed horse muscle cells and immune cell migration toward them. Methods: The expression patterns of the TLRs (TLR2, TLR4, and TLR8) and downstream signaling pathway-related genes (myeloid differentiation primary response 88 [MYD88]; activating transcription factor 3 [ATF3]) are examined in horse tissues, and horse peripheral blood mononuclear cells (PBMCs), polymorphonuclear cells (PMNs) and muscles in response to exercise, using the quantitative reverse transcription-polymerase chain reaction (qPCR). Expressions of chemokine receptor genes, i.e., C-X-C motif chemokine receptor 2 (CXCR2) and C-C motif chemokine receptor 5 (CCR5), are studied in PBMCs and PMNs. A horse muscle cell line is developed by transfecting SV-T antigen into fetal muscle cells, followed by examination of muscle-specific genes. Horse muscle cells are treated with stressors, i.e., cortisol, hydrogen peroxide (H₂O₂), and heat, to mimic stress conditions in vitro, and the expression of TLR4 and TLR8 are examined in stressed muscle cells, in addition to migration activity of PBMCs toward stressed muscle cells. Results: The qPCR revealed that TLR4 message was expressed in cerebrum, cerebellum, thymus, lung, liver, kidney, and muscle, whereas TLR8 expressed in thymus, lung, and kidney, while TLR2 expressed in thymus, lung, and kidney. Expressions of TLRs, i.e., TLR4 and TLR8, and mediators, i.e., MYD88 and ATF3, were upregulated in muscle, PBMCs and PMNs in response to exercise. Expressions of CXCR2 and CCR5 were also upregulated in PBMCs and PMNs after exercise. In the muscle cell line, TLR4 and TLR8 expressions were upregulated when cells were treated with stressors such as cortisol, H₂O₂, and heat. Migration of PBMCs toward stressed muscle cells was increased by exercise and oxidative stresses, and combinations of these. Treatment with methylsulfonylmethane (MSM), an antioxidant on stressed muscle cells, reduced migration of PBMCs toward stressed muscle cells. Conclusion: In this study, we have successfully cultured horse skeletal muscle cells, isolated horse PBMCs, and established an in vitro system for studying stress-related gene expressions and function. Expression of TLR4, TLR8, CXCR2, and CCR5 in horse muscle cells was higher in response to stressors such as cortisol, H₂O₂, and heat, or combinations of these. In addition, migration of PBMCs toward muscle cells was increased when muscle cells were under stress, but inhibition of reactive oxygen species by MSM modulated migratory activity of PBMCs to stressed muscle cells. Further study is necessary to investigate the biological function(s) of the TLR gene family in horse muscle cells.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.22 no.3
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• pp.44-50
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• 2021

This study is a descriptive research study to determine the effect of job stress and job satisfaction of 119 paramedics on their job performance ability. The subjects were 119 paramedics working at the fire department who agreed to participate in the study, and data collection was conducted from July 1st to September 10th, 2020. Data analysis was performed using the SPSS 23.0 program, with frequency and percentage, mean and standard deviation, correlation analysis, t-test, and ANOVA. As a result, the average job stress, job satisfaction, and job performance ability of 119 paramedics were 2.85±0.29 points, 3.35±0.30 points, and 3.53±0.89, respectively. A higher job satisfaction indicated higher job performance ability. An examination of the characteristics of the variables according to the general characteristics showed that the job performance ability was related significantly to the marital status, age, and first aid experience. Based on this, to establish a positive professional intuition to increase the job performance ability of 119 paramedics, lower job stress, and increases job satisfaction, it is important to raise awareness of job satisfaction, establish a job view, and promote job satisfaction through performance improvement. Program development and education programs for children should be actively carried out.rough performance improvement. Program development and education programs for children should be actively carried out.

• Korean Journal of Culture and Social Issue
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• v.21 no.3
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• pp.299-315
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• 2015

This study examines correlations among factors that reportedly affect adjustment to military life: self esteem, locus of control,, social support and stress coping strategies. The study also attempts to validate the structural model to enhance understanding of the process of adjusting to the military life. The subjects of the study were 447 listed soldiers from private to sergeant currently stationed and serving in an army post in Gangwon-Do, Korea. The following tools were used for this study. Based on self-inventory questionnaires, evaluations were made regarding self esteem, locus of control, social support, stress management, adaptation to military life. Reliability of each criterion was evaluated based on reliability examination, correlations among parameters were analyzed, and the structural equation model was validated to verify the research model. The results of the study can be summarized as follows. First, internal control and self esteem have significant positive influence on social support and stress coping. Second, internal control and self esteem affect adjusting to military life through social support and active coping, two factors mediated in this study to act as parameters between self-esteem and internal control and military adaption. This study emphasizes intervention such as establishment of a social support system and training for taking necessary actions as limitations of studies dealing with internal traits and military adaptation. Since the subjects of this study were chosen from an army based in a single location for convenience' sake, it may be difficult to generalize the results to analyze the entire military.

• Journal of the Korean Geographical Society
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• v.42 no.4
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• pp.648-665
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• 2007

The present curricula of department of common social studies education and department of geography education have the problem including only the basic completion subjects, and not including their principal themes. This unestablished state of principal themes in the basic completion subjects has given rise to the absent state of standard curriculum in department of common social studies education and department of geography education, and that finally has aroused many obstacles in the problem-setting and the examination-applying of middle and high school teacher appointment examination. Accordingly, this study established the principal themes of 21 basic completion subjects in common social studies and geography. The principal themes of 21 basic completion subjects in common social studies and geography will be form the base of establishing the content element of standard curriculum in department of common social studies education and department of geography education and the assessment element of middle and high school teacher appointment examination in common social studies and geography as an indication subject for examination of the teacher's qualification. Especially, considering the curriculum modification of college of education and the system change of middle and high school teacher appointment examination due to be propulsed simultaneously by Ministry of Education to the year of 2009, the establishment of principal themes in the basic completion subjects and the making of standard curriculum are very pertinent to the occasion. Because the principal themes of 21 basic completion subjects established in this study are made for some 50 days driven by the stress of circumstances, those have to be studied again. Also, because the construction of basic completion subjects includes many problems, that has to be revised.