• Journal of the korean academy of Pediatric Dentistry
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• v.42 no.4
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• pp.331-339
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• 2015

The first priority of sedation for incorporative children in pediatric dentistry is a safety. Therefore, evidence-based practices in health care are needed for preventing medical accidents. In accordance with the rise of the evidence based medicine, the interest in Evidence-Based Dentistry is increasing in the field of dentistry. However, systematic research about Evidence-Based sedation in Korea has rarely been done. As such, the purpose of this systematic review is to critically analyze the available scientific literature regarding dental sedation and to seek the next developmental strategies about evidence based pediatric dental sedation. A broad search of the 5 databases of the systematic reviews manual of the National Evidence-based Healthcare Collaborating Agency in Korea were referenced: 1) Core search database- KMbase, KISS; 2) Academic information and portal; 3) the National Assembly Library; 4) DBpia, and 5) RISS. Of a total 470 themes limited to the search term of "dental sedation", in accordance with the PRISMA statement for reporting systematic reviews of health sciences interventions, a literature selection process, which includes the removal of overlapping down the flow chart, was performed. Of the remaining 31 articles, two authors read through articles independently and added or removed articles using the exclusion criteria. Finally, twenty published papers of acceptable quality were identified and reviewed. This systemic review of Korean pediatric dental sedation practices for the last twenty-five years was based on the objective criteria defined in the GRADE process and identified consistent evidence. The results were evidence of moderate quality. Therefore, more systemically well-designed clinical studies are needed about the safe use of a sedative medicines (drugs).

• The Journal of the Society of Korean Medicine Diagnostics
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• v.11 no.2
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• pp.13-26
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• 2007

Background: Neurofeedback is an electroencephalographic biofeedback technique for training individuals to alter their brain activity via operant conditioning. Also neurofeedback is a form of behavioural training aimed at developing skills for brain activity. Within the past decade, several neurofeedback studies have been published that tend to overcome the methodological shortcomings of earlier studies. This research describes the methodical basis of neurofeedback and reviews the evidence base for its clinical efficacy and effectiveness in attention-deficit hyperactivity disorder (ADHD). Methods: In neurofeedback training, self-regulation of specific aspects of electrical brain activity is acquired by means of immediate feedback and positive reinforcement. In frequency training, activity in different EEG frequency bands has to be decreased or increased. Slow cortical potentials (SCPs) training is focused on the regulation of cortical excitability. Results: Neurofeedback studies revealed training-specific effects on, for example, attention and memory processes and performance improvements in real-life conditions, in healthy subjects as well as in patients. In several studies it was shown that ADHD symptomatology was reduced after frequency training or SCP(Slow cortical potentials) training. Moreover a decrease of impulsivity errors and an increase of the contingent negative variation. Conclusions: This research provides evidence for both positive behavioural and specific neurophysiological effects of neurofeedback training. Also there is growing evidence for neurofeedback as a valuable module in neuropsychiatric disorders. Further, controlled studies are warranted.

• The Journal of the Korea Contents Association
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• v.12 no.3
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• pp.214-221
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• 2012

In recent years, some researchers reported that myofascia was innervated by the autonomic nervous system. However, there is no neurophysiological explanation and evidence for the effects of myofascial release(MFR). Thus, the aim of this study was to determine whether the excitability of the autonomic nervous system is modulated by MFR. In this study, thirty healthy subjects in their 20s were randomly assigned to a myofascial release group(MG) and a placebo control group(PCG); each group had 15 subjects. The MG conducted 5 minutes of cranial base release in supine position, and the PCG performed sham cranial base release. Muscle flexibility was measured with the neck range of motion and the changes of the autonomic nervous system excitability was measured by heart rate, blood pressure, and concentration of plasma epinephrine and norepinephrine. The results were as follows: 1. The percentage changes in the cervical range of motion for extension and side flexion were significantly increased in the MG, signifying that more muscle relaxation. 2. There was no significant percentage changes in heart rate, blood pressure, and concentration plasma epinephrine between MG and PCG. 3. The percentage change in concentration plasma norepinephrine was significantly different between MG and PCG. The result of this study suggests that there is no evidence that MFR can modulate the autonomic nervous system excitability.

• Journal of Plant Biotechnology
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• v.44 no.2
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• pp.107-114
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• 2017

Implementation of crop improvement programs relies on genetic diversity. To overcome the limited occurrence of natural mutations, researchers and breeders applied diverse methods, ranging from conventional crossing to classical bio-technologies. Earlier generations of knockout and gain-of-function technologies often result in incomplete gene disruption or random insertions of transgenes into plant genomes. The newly developed editing tool, CRISPR/Cas9 system, not only provides a powerful platform to efficiently modify target traits, but also broadens the scope and prospects of genome editing. Customized Cas9/guide RNA (gRNA) systems suitable for efficient genomic modification of mammalian cells or plants have been reported. Following successful demonstration of this technology in mammalian cells, CRISPR/Cas9 was successfully adapted in plants, and accumulating evidence of its feasibility has been reported in model plants and major crops. Recently, a modified version of CRISPR/Cas9 with added novel functions has been developed that enables programmable direct irreversible conversion of a target DNA base. In this review, we summarized the milestone applications of CRISPR/Cas9 in plants with a focus on major crops. We also present the implications of an improved version of this technology in the current plant breeding programs.

• Korean Journal of Veterinary Research
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• v.47 no.3
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• pp.309-314
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• 2007

A 7-year-old, intact female Korean Jindo dog was referred due to ataxia and pain on palpation of the thorax. Radiograph, echocardiography and computed tomogram revealed a mass on the heart base region and osteolytic change of fifth thoracic (T5) vertebra. At necropsy, a firm, encapsulated and round mass was seen arising from the heart base region surrounding the ascending aorta and pulmonary artery. Histopathologically, nests of cuboidal and polyhedral cells having abundantly granular and eosinophilic cytoplasm with round to oval nucleus were separated by fibrous septa. Immunohistochemistry using chromogranin A revealed that tumor cells were originated from neuroendocrine organ and metastasized into some organs including lung, spleen, liver, kidney and T5 vertebra. By electron microscopy, we found the electron-dense and membrane-bound granules in cytoplasm of the tumor cells. This study provides the uncommon evidence that aortic body tumor metastasized to both multiple organs and bone.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.34 no.4
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• pp.450-455
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• 2008

A Single Nucleotide Polymorphism (SNP) is a small genetic change or variation that can occur within a DNA sequence. It's the difference of one base at specific base pair position. SNP variation occurs when a single nucleotide, such as an A, replaces one of the other three nucleotide letters-C, G, or T. On average, SNP occur in the human population more than 1 percent of the time. They occur once in every 300 nucleotides on average, which means there are roughly 10 million SNPs in the human genome. Because SNPs occur frequently throughout the genome and tend to be relatively stable genetically, they serve as excellent biological markers. They can help scientists locate genes that are associated with disease such as heart disease, cancer, diabetes. They can also be used to track the inheritance of disease genes within families. SNPs may also be associated with absorbance and clearance of therapeutic agents. In the future, the most appropriate drug for an individual could be determined in advance of treatment by analyzing a patient's SNP profile. This pharmacogenetic strategy heralds an era in which the choice of drugs for a particular patient will be based on evidence rather than trial and error (so called "personalized medicine").

• Journal of Microbiology
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• v.41 no.1
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• pp.52-57
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• 2003

The mutational spectra in the manganese (II) peroxidase gene (mnp) of the Pleurotus ostreatus mutants induced by gamma radiation (Co$\^$60/) give evidence to prove the effect of gamma radiation on the gene. mnp of each mutant was cloned, sequenced and analyzed. Among the 1941 base pairs of the sequenced region of the mnP genes of 4 mutants (PO-5,-6,-15 and -16), nine mutational hotspots on which the same base was mutated simultaneously were found, additionally 6 mutations were also found at different positions in the mnp gene. These mutation-spectra were predominantly A:T\longrightarrowG:C transitions (50.1%). By the analysis of putative amino acid sequences, PO-5 and PO-16 mutants have 3 and 1 mutated residues, respectively. Since the mutational spectra reported herein are specific to the mnp gene, we propose that the mutational hotspots for the gamma radiation could be in the gene(5) within cells.

• Journal of Veterinary Clinics
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• v.35 no.1
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• pp.26-29
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• 2018

A 6-month-old, female poodle presented with a three-month history of persistent regurgitation immediately after eating. On physical examination, the patient was emaciated and dehydrated. Thoracic radiography showed ventral displacement of the trachea and increased radiopacity in the mediastinum, cranial to the heart base. A severely dilated esophagus was identified cranial to the heart on esophagram. Computed tomography (CT) revealed the esophagus was filled with gas, fluid and a little of contrast and dilated from caudo-cervical to cranio-thoracic part. The esophageal diameter was markedly decreased at the heart base. In addition, the trachea was displaced to the left-ventral side of the right aortic trunk and an aberrant left subclavian artery originating from the aorta was identified. There was no evidence of abdominal vascular anomaly. Based on diagnostic imaging, persistent right aortic arch (PRAA) with an aberrant left subclavian artery was diagnosed. The patient did not undergo surgery and died at 15 days after diagnosis. This report describes imaging diagnosis, including CT and radiography in a weaned dog with regurgitation due to esophageal obstruction by PRAA. When PRAA is suspected and conventional radiography or contrast study is insufficient for diagnosis, CT may be helpful for diagnosing PRAA.

• The Journal of Korean Academy of Sensory Integration
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• v.20 no.3
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• pp.48-59
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• 2022

Objective : This study was intended to systematically consider the sensory integration intervention methods offered in Korea for children with autism spectrum disorder and to provide an evidence base for the application of sensory integration interventions. Methods : The subjects of this study were published studies in a national journal for the last 10 years from January 2011 to December 2020. The databases used for the search were RISS and DBpia. The search terms were 'autism', 'autism spectrum', 'sensory integration', and 'intervention'. A total of 10 studies were used in the analysis, which were analyzed at the qualitative and methodological quality of the research evidence and the results were presented according to the PICO (Patient, Intervention, Comparison, Outcome). Results : In the research analysis, the quality level of the evidence was highest at level IV, followed by level II. The methodological quality of the evidence was the most common for 'Good' research, followed 'Fair'. The study subjects were children with autism spectrum disorder and their parents, and the experimental design had the highest frequency of single-subject studies. Interventions have the highest number of studies confirming adaptive behavior and sensory modulation, and the evaluation tools used to measure interventions have the highest frequency of sensory profiles and Canadian occupational performance measures (COPM). All 10 studies used in the analysis showed positive improvements and statistically significant effects on various outcome values from sensory integration interventions. Conclusion : In the recent clinical environment, sensory integration interventions have been continuously conducted in children with autism spectrum disorder. In future research, it is necessary to conduct research on various sensory integration intervention methods and the high quality of the evidence for the application of sensory integration interventions.

• Bulletin of the Korean Chemical Society
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• v.33 no.4
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• pp.1165-1169
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• 2012

The fluorescence intensity of DNA-intercalated ethidium with [ethidium]/[DNA base] being 0.005 was quenched upon the binding of another intercalating ligand, meso-tetrakis(N-methylpyridinium-4-yl)porphyrin (TMPyP). Addition of $Ca^{2+}$ enhanced the quenching efficiency. The range of separations between donor and acceptor molecules, within which total quenching occurs, was calculated using a one-dimensional resonance energy transfer mechanism to be 9.5 base-pairs or $32.3{\AA}$ in the absence of $Ca^{2+}$ ions. The distance increased to 18.7 base-pairs or about $63.6{\AA}$ in the presence $100{\mu}M$ $Ca^{2+}$. Considering that (1) $Ca^{2+}$ had little effect on the binding modes of ethidium and TMPyP, which was investigated by reduced linear dichroism and (2) spectral overlap between the emission spectrum of ethidium and the absorption spectrum of TMPyP was maintained in the presence of $Ca^{2+}$, contributions from orientation factor and spectral overlap to $Ca^{2+}$-induced enhancement in DNA mediated energy transfer was limited. Although there is no direct evidence, electron transfer along the DNA stem may accompany the observed fluorescence quenching. In this respect, DNA bound $Ca^{2+}$ act as a partially conducting medium.