Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Mutation Spectrum of Manganese (II) Peroxidase Gene in the Pleurotus ostreatus Mutants Induced by Gamma Radiation

  • Chang, Hwa-Hyoung (Research Center for Bio-Medicinal Resource, PaiChai University) ;
  • Lee, Young-Keun (Radiation Application Division, Korea Atomic Energy Research Institute) ;
  • Kim, Jae-Sung (Radiation Application Division, Korea Atomic Energy Research Institute) ;
  • Lee, Ki-Sung (Research Center for Bio-Medicinal Resource, PaiChai university) ;
  • Cho, Kyu-Seong (Radiation Application Division, Korea Atomic Energy Research Institute)
  • Published : 2003.03.01
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Abstract

The mutational spectra in the manganese (II) peroxidase gene (mnp) of the Pleurotus ostreatus mutants induced by gamma radiation (Co$\^$60/) give evidence to prove the effect of gamma radiation on the gene. mnp of each mutant was cloned, sequenced and analyzed. Among the 1941 base pairs of the sequenced region of the mnP genes of 4 mutants (PO-5,-6,-15 and -16), nine mutational hotspots on which the same base was mutated simultaneously were found, additionally 6 mutations were also found at different positions in the mnp gene. These mutation-spectra were predominantly A:T\longrightarrowG:C transitions (50.1%). By the analysis of putative amino acid sequences, PO-5 and PO-16 mutants have 3 and 1 mutated residues, respectively. Since the mutational spectra reported herein are specific to the mnp gene, we propose that the mutational hotspots for the gamma radiation could be in the gene(5) within cells.

Keywords

References

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