• Environmental Analysis Health and Toxicology
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• v.15 no.3
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• pp.99-106
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• 2000

Ethylenediaminetetraacetic acid (EDTA) and nitrilotriacetic acid (NTA) are various applied as chelating agents for metal ions, then they are widely used in many industrial processes and domestic products. A method is described for the determination of EDTA and NTA in water samples by GC/MS . The reaction temperature, reaction time and pH for esterification of EDTA and NTA were investigated using 10% sulfuric acid-methanol, ethanol and propanol. Optimum conditions were obtained by the esterification in 80$\^{C}$ for 1hr with ethanol. Method detection limits of ethylated EDTA and NTA in the 200 ml of water samples were 0.05 ng/ml, respectively, EDTA and NTA could be determined in the range of 0.05∼23.6 and 0.05∼7.0 ng/ml in treated water, and in the range of 0.06∼25.0 and 0.05∼6.40 ng/ml in raw water respectively. Risk assessments with EDTA and NTA exposure by drinking water ingestion were carried out. Based on the results of analysis, chronic daily intakes of EDTA and NTA would be less than the value of acceptable daily intake or tolerable daily intake.

• Journal of environmental and Sanitary engineering
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• v.13 no.2
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• pp.95-105
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• 1998

This research was investigated which denitrification of wastewater containing nitrate, using upflow anaerobic sludge blanket process. The upflow anaerobic sludge blanket process is also used for both artifical and industrial wastewater. Main ingredients investigated in the artifical and industrial wastewater experiment were the determination of optimum organism/nitrate ratios, nitrate removal efficiency by various hydrogen donor addition and characteristics of granular sludge and gas production in case of various hydrogen donor addition. From the experimental results the following conclusions were made: In case of adding methanol, ethanol and sodium acetate as hydrogen donor granular sludge was formed 50 days after seeding. Average diameter of granular sludge was 4.0 mm and settling velocity was 37 cm/min. Production rate of gas 3.3 L/d in case of adding methanol as hydrogen donor in wastewater containing 150mg/L nitrate. However adding ethanol and sodium acetate as hydrogen donor, gas production rate were 2.2-2.7L/d respectively. In case of adding methanol as hydrogen donor treatability of artifical wastewater contained 150mg/L as nitrate was about 93%. But in addition of sodium acetate in wastewater contained 40mg.L as nitrate, nitrate removal efficiency was 80%.

• Journal of Korean Society on Water Environment
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• v.21 no.6
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• pp.630-636
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• 2005

Groundwater contamination by nitrate exceeding water quality criteria (10 mg $NO_3{^-}-N/L$) occurs frequently. Fumarate, acetate, formate, lactate, propionate, ethanol, methane and hydrogen gas were evaluated for their nitrate removal efficiencies and removal rates for in situ bioremediation of nitrate contaminated groundwater. Denitrification rate for each substrate was in the order of: fumarate > hydrogen > formate/lactate > ethanol > propionate > methanol > acetate. Microcosm studies were performed with fumarate and acetate. When fumarate was used as a substrate, nitrate was removed 100 percent with rate of 0.66 mmol/day while conversion rate from nitrate to nitrogen gas or another by-product was 87 percent. 42 mg of fumarate was needed to remove 30 mg $NO_3{^-}-N/L$. When using acetate as carbon source, 31 percent of nitrate was removed during initial adjustment period. Among removed fraction, however, 83 percent of nitrate removed by cell growth. Overall nitrate removal rate was 0.37 mmol/day. Acetate showed longer lag time in consumption compared to that of nitrate, which implying that acetate would be better carbon source compared to fumarate as more amount was utilized for nitrate removal than cell growth.

• Korean Journal of Food Science and Technology
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• v.27 no.2
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• pp.266-269
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• 1995

In order to measure alcohol contents with speed and accuracy, alcohol sensor was prepared. Alcohol sensor was made by connecting with oxygen electrode after immobilized alcohol oxidase on nylon net with glutaraldehyde. Alcohol was determined by changing the rate of dissolved oxygen consumption using D.O. analyzer. Alcohol contents in alcoholic beverages were determined under the optimum conditions. The results were 0.71% in low-alcohol beverage, $4{\sim}5%$ in beers, 10.06% in wine, 16.12% in chungju, 25.71% in soju, and 6.18% in takju, respectively. The values by alcohol sensor showed an excellent correlation(r=0.999) with GC method.

• Analytical Science and Technology
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• v.16 no.2
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• pp.95-101
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• 2003

In this study, water content in the mixture of methanol and ethanol was nondestructively measured by near infrared (NIR) spectroscopy. Two types of NIR instruments, portable NIR system with a photo-diode array and scanning type NIR spectrometer were used and the calibration results were compared. Partial least squares regression (PLSR) was applied for the calibration and validation for the quantitative analysis. The calibration results from both instruments showed good correlation with actual values. The calibration with the use of PLS model predicted water concentration with a standard error of prediction (SEP) of 0.10% and 0.12% for photo diode array and scanning type, respectively. During 6 days, routine analyses for 3%, 5% and 7% water in ethanol solution with 2% methanol were performed to validate the robustness of the developed calibration model. The routine analyses showed good results with coefficient of variation (CV) of within 3% for both types of NIR spectrometers. This study showed that the rapid determination of water in the mixture of methanol and ethanol was successfully performed by NIR spectroscopy and the performance of the portable NIR system with a photo diode array detector was comparable to that of the scanning type NIR spectrometer.

• KSBB Journal
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• v.21 no.5
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• pp.371-375
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• 2006

Frontal analysis(FA) and Pulsed input method(PIM) have been frequently utilized to measure isotherm of single solute, as well as non-competitive isotherms of two solutes in chromatography(1). FA and PIM were used in this study as complementary methods to measure adsorption isotherms of loxoprofen racemate in HPLC. Prior to FA and PIM experiments, measurements of loxoprofen solubility were made at hexane/ethanol=50/50, 80/20, 95/5(v/v) with acetic acid(0.5%) for adjusting pH. The last composition(95/5) of hexane/ethanol allows us to separate loxoprofen racemate into two forms(retentate, extract). PIM and FA were used to determine the isotherms of re-and ex-loxoprofen.

• Korean Journal of Food Science and Technology
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• v.41 no.5
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• pp.572-577
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• 2009

The effects of cereal (rice, brown rice, barley, glutinous rice) ethanol extracts on the proliferation, nitric oxide (NO) production, and level of tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$) in B and T lymphocytes, isolated from human blood cells of sasang constitution, were examined in vitro. The rice, brown rice, and glutinous rice ethanol extract, which are known as suitable for eumin (taeumin and soeumin) constitutions, showed the highest activities for proliferation, NO production, and TNF-${\alpha}$ concentration, respectively. However, the barley ethanol extract, which is known tobe suitable for yangin (taeyangin and soyangin) constitutions, showed the highest overall activities for proliferation, NO production, and TNF-${\alpha}$ concentration. The different immunological activities of the cereal extracts in sasang constitution lymphocytes might be due to their individual components. Thus, determination of components from the cereal extracts suitable for sasang constitution could be useful in new food developments.

• Journal of the Korean Chemical Society
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• v.51 no.1
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• pp.21-30
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• 2007

A multiwavelength spectrophotometric titration method was applied to study protolytic constants of four water-soluble vitamins, folic acid(vitamin B9 or B0), thiamine(vitamin B1), riboflavin(vitamin B2) and pyridoxal (vitamin B6) in binary ethanol-water mixtures at 25oC and an ionic strength of 0.1M NaNO3. The protolytic equilibrium constants, spectral profiles, concentration diagrams and also the number of components has been calculated from the curve fitting of the pH-absorbance data with appropriate mass balance equations by an established factor analysis model. DATAN program was used for determination of acidity constant and SPECFIT program was used for calculation of standard deviations and partial correlation coefficients. A glass electrode calibration procedure based on the four parameter equation pH=α+SpcH+JH+[H+]+ JOH-Kw/[H+] based on the Gran,s plots was used to obtain pH-readings in the concentration scale (pcH). The effect of the solvent on the protolytic constants was discussed.

• The Korean Journal of Food And Nutrition
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• v.31 no.1
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• pp.104-108
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• 2018

The purpose of this study was to establish valerenic acid as a marker compound for the standardization of ethanol extract of Valerinan officinalis (valerian) root as a functional health food. We established valerenic acid as a marker compound using HPLC. HPLC was used to quantify the marker compound in the valerian extract after validation of methods with linearity, accuracy, and precision. The specificity for retention time was met by comparative analysis of the valerian extract and standard compound using HPLC. The method showed high linearity of the calibration curve with a coefficient of correlation ($R^2$) of 0.9999. The limit of quantification (LOQ) was $10{\mu}g/mL$. The accuracy of measurement was 99.88~00.68% and the relative standard deviation (RSD) value was 0.59%. In addition, our analytical method yielded a 29% mean content of valerenic acid in the valerian ethanol extract. These results indicate that the established HPLC method facilitated the determination of marker compounds in the valerian extract for the standardization of health functional foods.

• The Korea Journal of Herbology
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• v.29 no.4
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• pp.77-82
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• 2014

Objectives : The effects of ethanol extract of Plantago asiatica L. were investgated on adipocyte differentiation, lipopogenesis, lipolysis and apoptosis using differnentiated 3T3-L1 adipocytes. Methods : Plantago asiatica L. was extracted with ethanol (CCE). We carried on MTT assay for cell proliferation, Oil Red O staining for determination of cell differentiation and intracelluar adipogenesis. TUNEL staining assay for cell apoptosis, and Western blot analysis for measurement of pAMPK and pACC, $C/EBP{\alpha}$, $PPAR{\gamma}$ protein expressions were performed. Results : The addition of CCE up to 0.2 mg/ml into cell culture media showed no cytotoxicity. Treatment of 0.2 mg/ml CCE significantly inhibited differentiation in 3T3-L1 preadipocytes. Lipid accumulation of the CCE treated cells was decreased compared with that of control. Induction of cell apoptosis was increased in CCE treated cells compared with that of control. AMPK and ACC levels of the cells with 0.2 mg/ml CCE were led to phosphorylation and also expressions of $C/EBP{\alpha}$ and $PPAR{\gamma}$, as adipogenic transcription factors, were suppressed compared with those of control. Conclusions : Taken together, these results provide evidence that CCE has a regulatory role in lipid metabolism that is related to differentiation into adipocytes, adipogenesis and apoptosis.