Browse > Article
http://dx.doi.org/10.9799/ksfan.2018.31.1.104

HPLC Validation of Valerian Ethanol Extract as a Functional Food  

Jo, Kyungae (Dept. of Integrated Biomedical and Life Science, Graduate School, Korea University)
Han, Sung Hee (BK21Plus, College of Health Science, Korea University)
Bae, Song-Hwan (Dept. of Food and Biotechnology, Hankyong National University)
Shin, Jung Cheul (Neo Cremar Co. Ltd.)
Suh, Hyung Joo (BK21Plus, College of Health Science, Korea University)
Publication Information
The Korean Journal of Food And Nutrition / v.31, no.1, 2018 , pp. 104-108 More about this Journal
Abstract
The purpose of this study was to establish valerenic acid as a marker compound for the standardization of ethanol extract of Valerinan officinalis (valerian) root as a functional health food. We established valerenic acid as a marker compound using HPLC. HPLC was used to quantify the marker compound in the valerian extract after validation of methods with linearity, accuracy, and precision. The specificity for retention time was met by comparative analysis of the valerian extract and standard compound using HPLC. The method showed high linearity of the calibration curve with a coefficient of correlation ($R^2$) of 0.9999. The limit of quantification (LOQ) was $10{\mu}g/mL$. The accuracy of measurement was 99.88~00.68% and the relative standard deviation (RSD) value was 0.59%. In addition, our analytical method yielded a 29% mean content of valerenic acid in the valerian ethanol extract. These results indicate that the established HPLC method facilitated the determination of marker compounds in the valerian extract for the standardization of health functional foods.
Keywords
Valerinan officinalis; valerenic acid; HPLC; validation; functional health food;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Leathwood PD, Chauffard FI. 1985. Aqueous extract of valerian reduces latency to fall asleep in man. Planta Med 51:144-148
2 Leuschner J, Muller J, Rudmann M. 1993. Characterisation of the central nervous depressant activity of a commercially available valerian root extract. Arzneimittelforschung 43: 638-641
3 Ross SM. 2009. Sleep disorders: A single dose administration of valerian/hops fluid extract (dormeasan) is found to be effective in improving sleep. Holist Nurs Pract 23:253-256
4 Shohet D, Wills RB, Stuart DL. 2001. Valepotriates and valerenic acids in commercial preparations of valerian available in Australia. Pharmazie 56:860-863
5 Tania L, Brian CF. 2004. In vitro activity of commercial valerian root extracts against human cytochrome P450 3A4. J Pharm Pharmaceut Sci 7:265-273
6 Yuan CS, Mehendale, Xiao YP, Aung HH, Xie JT, Ang MK. 2004. The gamma-aminobutyric acidergic effects of valerian and valerenic acid on rat brainstem neuronal activity. Anesth Analg 98:353-358
7 Donovan JL, Devane CL, Chavin KD, Wang JS, Gibson BB, Gefroh HA, Markowitz JS. 2004. Multiple night-time doses of valerian (Valeriana officinalis) had minimal effexts on CYP3A4 activity and no effect on CYP2D6 activity in healthy volunteers. Drug Metab Dispos 32:1333-1336
8 Becker A, Felgentreff F, Schroder H, Meier B, Brettstrom A. 2014. The anxiolytic effects of a valerian extract is based on valerenic acid. BMC Complement Altern Med 14:267
9 Debnath T, Park DK, Lee BR, Jin HL, Lee SY, Samad NB, Lim BO. 2013. Antioxidant activity of inonotus obliquus grown on germinated brown rice extracts. J Food Biochem 37: 456-464
10 Donath F, Quispe S, Diefenbach K, Maurer A, Fietze I, Roots I. 2000. Critical evaluation of the effect valerian extract on sleep structure and sleep quality. Pharmacopsychiatry 33: 47-53
11 Gray WS. 2005. Use of solid phase extraction for improving HPLC assays of triterpene glycosides in gotu kola (Centella asiatica), parthenolide in feverfew (Tanacetum parthenium), and valerenic acids in valerian (Valeriana officinalis). J Liq Chromatogr Relat Technol 28:581-592
12 Goppel M, Franz G. 2004. Stability control of valerian ground material and extracts: A new HPLC-method routine quantification of valerenic acids and lignans. Pharmazie 59:446-452
13 Hintersteiner J, Haider M, Luger D, Schwarzer C, Reznicek GM, Jager W, Khom S, Mihovilovic MD, Hering S. 2014. Esters of valerenic acid as potential prodrugs. Eur J Pharmacol 735:123-131