Browse > Article
http://dx.doi.org/10.6116/kjh.2014.29.4.77.

Ethanol extract of Plantago asiatica L. controls intracellular fat accumulation and lipid metabolism in 3T3-L1 Adipocytes  

Jeon, Seo Young (Team for Scientification of Korean Medical Intervention (BK21 Plus))
Park, Ji Young (Dept. of Herbal Pharmacology, Coll. of Oriental Medicine, Daegu Haany Univ.)
Shin, Insoon (Dept. of Herbal Pharmacology, Coll. of Oriental Medicine, Daegu Haany Univ.)
Kim, Sung Ok (Dept. of Herbal Pharmacology, Coll. of Oriental Medicine, Daegu Haany Univ.)
An, Hee Duk (Dept. of Oriental Rehabilitation Medicine, Coll. of Oriental Medicine, Daegu Haany Univ.)
Kim, Mi Ryeo (Team for Scientification of Korean Medical Intervention (BK21 Plus))
Publication Information
The Korea Journal of Herbology / v.29, no.4, 2014 , pp. 77-82 More about this Journal
Abstract
Objectives : The effects of ethanol extract of Plantago asiatica L. were investgated on adipocyte differentiation, lipopogenesis, lipolysis and apoptosis using differnentiated 3T3-L1 adipocytes. Methods : Plantago asiatica L. was extracted with ethanol (CCE). We carried on MTT assay for cell proliferation, Oil Red O staining for determination of cell differentiation and intracelluar adipogenesis. TUNEL staining assay for cell apoptosis, and Western blot analysis for measurement of pAMPK and pACC, $C/EBP{\alpha}$, $PPAR{\gamma}$ protein expressions were performed. Results : The addition of CCE up to 0.2 mg/ml into cell culture media showed no cytotoxicity. Treatment of 0.2 mg/ml CCE significantly inhibited differentiation in 3T3-L1 preadipocytes. Lipid accumulation of the CCE treated cells was decreased compared with that of control. Induction of cell apoptosis was increased in CCE treated cells compared with that of control. AMPK and ACC levels of the cells with 0.2 mg/ml CCE were led to phosphorylation and also expressions of $C/EBP{\alpha}$ and $PPAR{\gamma}$, as adipogenic transcription factors, were suppressed compared with those of control. Conclusions : Taken together, these results provide evidence that CCE has a regulatory role in lipid metabolism that is related to differentiation into adipocytes, adipogenesis and apoptosis.
Keywords
Plantago asiatica L; differentation; adipogenesis; apoptosis; anti-obesity;
Citations & Related Records
Times Cited By KSCI : 7  (Citation Analysis)
연도 인용수 순위
1 Yook CS. Korean medicinal plants. Academy publishing. 1989 : 502.
2 Song HJ. Healthy native grass therapy. Gookillmedia. 1994 : 142.
3 Yang KH, Kwon TJ, Choo SY, Yun HS, Chang IM. Protective effect of Aucuba japonica against CCl4-induced liver damage in rats. Drug Chem Toxicol. 1983 ; 6(5) : 429-41.   DOI
4 Chang IM, Ryu JC, Yun HS, Yang KW. Protective activites of aucubin against CCl4-induced liver damage in mice. Drug Chem Toxicol. 1983 ; 6(5) : 443-53.   DOI
5 Kurt J. Harrison's Principles of internal medicine. New York : Isselbacher. 1994 : 446-52.
6 Bray MS. Genomics, genes, and environmental interaction: the role of exercise. J Appl Physiol. 1985 ; 88(2) : 788-92.
7 Sharrett AR, Ballantyne CM, Coady SA, Heiss G, Sorlie PD, Catellier D, Patsch W. Coronary heart disease prediction from lipoprotein cholesterol levels, triglycerides, lipoprotein(a), apolipoproteins A-I and B, and HDL density subfractions: the atherosclerosis risk in communities (ARIC) study. Circulation. 2001 ; 104(10) : 1108-13.   DOI   ScienceOn
8 Huh KB. Symposium: recent progress in obesity research: pathogenesis of obesity. Kor J Soc Food Sci Nutr. 1990 ; 23 : 333-6.
9 Woo HC, Shin BK, Cho IJ, Kim MH, Han JH. Bioactive Materials : anti-obestiy effect of carbon dioxide supercritical fluid extracts of pannax ginseng ca meyer. J Korean Soc Appl Biol Chem. 2011 ; 54(5) : 738-43.   과학기술학회마을   DOI
10 Koutnikova H, Auwerx J. Regulation of adipocyte differentiation. Ann Med. 2001 ; 33 : 556-61.   DOI   ScienceOn
11 Rosen ED, Spiegelman BM. Molecular regulation of adipogenesis. Annu Rev Cell Dev Biol. 2000 ; 16 : 145-71.   DOI   ScienceOn
12 Park JA, Jin KS, Oh YN. Antiadipogenic effect of vitis amurensis root methanol extract and its solvent fractions in 3T3-L1 preadipocytes. J Life Sci. 2013 ; 23 : 69-78.   과학기술학회마을   DOI   ScienceOn
13 Morrison RF, Farmer SR. Hormonal signaling and transcriptional control of adipocyte differentiation. J Nutr. 2000 ; 130(12) : 3116-21.
14 Ntambi JM, Kim YC. Adipocyte differentiation and gene expression. J Nutr. 2000 ; 130 : 3122-6.   DOI
15 Wu Z, Rosen ED, Brun R, Hauser S, Adelmant G, Troy AE, McKeon C, Darlington GJ, Spiegelman BM. Cross-regulation of $C/EBP\alpha$ and PPAR $\gamma$ controls the transcriptional pathway of adipogenesis and insulin sensitivity. Mol Cell. 1999 ; 3(2) : 151-8.   DOI   ScienceOn
16 Kim HJ, Kang CH, Kim SK. Anti-adipogenic effect of undaria pinnatifida extracts by ethanol in 3T3-L1 adipocytes. J Life Sci. 2012 ; 22 : 1052-6.   과학기술학회마을   DOI   ScienceOn
17 Choi KH. Obesity. Seoul : Shinwonmonhwasa. 2002 : 15-44.
18 Park YW. Supplements used for weight loss of the evidence-based prescribing. J Korean Acad Fam Med. 2003 ; 24(5) : 409-15.
19 Park CY. The Update of Anti-Obesity Drug. Med Kyunghee. 2002 ; 18(2) : 77-85.
20 Sjostrom L, Lonn L, chowdhury B, grangard LL, Sjostrom D, Sullivan L. The sagirral diameter is a valid maker of the visceral adipose tissue volume. J Progress Obes. 1994 ; 7 : 309-19.
21 Sjostrom LV. Morbidity of severely obese subject. Am J Clin Nutr. 1992 ; 55 : 508-13.
22 Bradford BL, Bruce MS. Towards a molecular understanding of adaptive thermogenesis. Nature. 2000 ; 404 : 652-60.   DOI
23 Munoz A, Guichard JP, Reginault P. Micronized fenofibrate. Atherosclerosis. 1994 ; 110 : 45-8.   DOI
24 Kim CM, Shin MK. Chinese herbology an unabridged dictionary. Seoul : Jungdam publishing. 1998 : 5209-13.
25 Shin IS, Choi HM, Ku SK, Kim MR. The effect of natural mixture supplementation on histopathological and histomorphometrical aspects in high fat diet-induced obese mice. Kor J Herbology. 2012 ; 27(4) : 53-8.   과학기술학회마을   DOI   ScienceOn
26 Guay DR. Micronized fenofibrate: a new fibric acid hypolipidemic agent. Ann Pharmacother. 1999 ; 33(10) : 1083-103.   DOI   ScienceOn
27 Hardic DG. The AMP-activated protein kinase pathway-new players upstream and downstream. J Cell Sei. 2004 ; 117(23) : 5479-87.   DOI   ScienceOn
28 Nam KC, Kim MS, Lee WJ, Jang PG, Han SM, Koh EH, Park JY, Lee KU. Hypothalamic ampk activity in diabetic rats. J Kor Diabetes Assoc. 2004 ; 28 : 468-77.
29 Kahn BB, Alquier T, Carling D, Hardie DG. AMP-activated protein kinase: ancient energy gauge provides clues to modern understanding of metabolism. Cell Metab. 2005 ; 1 : 15-25.   DOI   ScienceOn
30 Park BG, Lee HS, Jung SH, Gu YC, Hong CE, Lee SJ, Lee GW. Single & 14-day repeated oral toxicity study and genotoxicological safety estimate of plantamajoside isolated from Plantago asiatica. J Toxicol Pub Health. 2007 ; 23(1) : 79-86.   과학기술학회마을   DOI   ScienceOn
31 Lee HY, Yoon KH, Seo BI, Park GR, Yoon MC, Shen ZB, Cui HH, Shin SS. Molecular biologic mechanism of obesity by GGEx18. Kor J Herbology. 2011 ; 26(1) : 65-74.   과학기술학회마을
32 Park JA, Park C, Han MH, Kim B W, Chung YH, Choi YH. Inhibition of adipocyte differentiation and adipogenesis by aged black garlic extracts in 3T3-L1 preadipocytes. J Life Sci. 2001 ; 21 : 720-8.   과학기술학회마을   DOI   ScienceOn