The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Ethanol extract of Plantago asiatica L. controls intracellular fat accumulation and lipid metabolism in 3T3-L1 Adipocytes

차전초의 에탄올추출물이 3T3-L1 지방세포의 지방축적 및 지질대사에 미치는 영향

  • Jeon, Seo Young (Team for Scientification of Korean Medical Intervention (BK21 Plus)) ;
  • Park, Ji Young (Dept. of Herbal Pharmacology, Coll. of Oriental Medicine, Daegu Haany Univ.) ;
  • Shin, Insoon (Dept. of Herbal Pharmacology, Coll. of Oriental Medicine, Daegu Haany Univ.) ;
  • Kim, Sung Ok (Dept. of Herbal Pharmacology, Coll. of Oriental Medicine, Daegu Haany Univ.) ;
  • An, Hee Duk (Dept. of Oriental Rehabilitation Medicine, Coll. of Oriental Medicine, Daegu Haany Univ.) ;
  • Kim, Mi Ryeo (Team for Scientification of Korean Medical Intervention (BK21 Plus))
  • 전서영 (한의치료기술과학화 사업팀(BK 21 Plus)) ;
  • 박지영 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 신인순 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 김성옥 (대구한의대학교 한의과대학 본초약리학교실) ;
  • 안희덕 (대구한의대학교 한의과대학 한방재활의학과) ;
  • 김미려 (한의치료기술과학화 사업팀(BK 21 Plus))
  • Received : 2014.06.15
  • Accepted : 2014.07.16
  • Published : 2014.07.30
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Abstract

Objectives : The effects of ethanol extract of Plantago asiatica L. were investgated on adipocyte differentiation, lipopogenesis, lipolysis and apoptosis using differnentiated 3T3-L1 adipocytes. Methods : Plantago asiatica L. was extracted with ethanol (CCE). We carried on MTT assay for cell proliferation, Oil Red O staining for determination of cell differentiation and intracelluar adipogenesis. TUNEL staining assay for cell apoptosis, and Western blot analysis for measurement of pAMPK and pACC, $C/EBP{\alpha}$, $PPAR{\gamma}$ protein expressions were performed. Results : The addition of CCE up to 0.2 mg/ml into cell culture media showed no cytotoxicity. Treatment of 0.2 mg/ml CCE significantly inhibited differentiation in 3T3-L1 preadipocytes. Lipid accumulation of the CCE treated cells was decreased compared with that of control. Induction of cell apoptosis was increased in CCE treated cells compared with that of control. AMPK and ACC levels of the cells with 0.2 mg/ml CCE were led to phosphorylation and also expressions of $C/EBP{\alpha}$ and $PPAR{\gamma}$, as adipogenic transcription factors, were suppressed compared with those of control. Conclusions : Taken together, these results provide evidence that CCE has a regulatory role in lipid metabolism that is related to differentiation into adipocytes, adipogenesis and apoptosis.

Keywords

References

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