• Korean Journal of Pharmacognosy
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• v.14 no.4
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• pp.131-136
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• 1983

The composition of the phenolic compounds of 14 species, 2 varieties and I taxon of the Sedum plant in Korea were studied to identify their inter-specific relationships and their taxonomical position. The phenograms and contour diagrams obtained from the HPLC of EtOAc soluble fraction of these plants were classified into 7 alliances according to their chemical patterns. These chemical patterns agreed with Satake's classification except for Sedum aizoon. These plants were subdivided into 5 alliances in the Sedum section and 2 alliances in the Hylotelephium section. I type of the Sedum spectabile showed a difference in the number of stamens, it could be trated as another category above variety according to this result.

• Natural Product Sciences
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• v.17 no.3
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• pp.212-215
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• 2011

In the course of screening anti-adipogenic activity of natural products employing the preadipocyte cell line, 3T3-L1 as an in vitro assay system, the EtOAc fraction of the stem barks of Acer tegmentosum Maxim (Aceraceae) showed significant inhibitory activity on adipocyte differentiation as assessed by measuring fat accumulation using Oil Red O staining. Activity-guided fractionation led to the isolation of active constituent, (+)-catechin. (+)-Catechin showed inhibitory activity on adipocyte differentiation in dose-dependent manner. Further studies with interval treatment demonstrated that (+)-catechin exerted inhibitory activity on adipocyte differentiation via acting on early stage of adipogenesis. Our present study also showed that (+)-catechin significantly inhibited the preadipocyte proliferation. Taken together, these results suggest that (+)-catechin, a constituent of A. tegmentosum might contribute the anti-adipogenic activity of A. tegmentosum.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1998.11a
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• pp.186-186
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• 1998

Persicaria lapathifolia Gray (Polygonaceae) is a common weed in Korea. This plant and other Persicaria species including P. orientale and P. pubescens have been used as an analgesic and stomachic as well as for the treatment of rheumatoid arthritis and malaria. During the screening program of plant extracts, MeOH extract of P. scabrum showed anticomplement activity and the MeOH extract was partitioned with hexane, chloroform, ethyl acetate, and buthanol. EtOAc fraction showed strong activity and activity guided separation yielded eight flavonoids. Two known galloylated and a novel ferulloylated flavonoid glycosides showed strong anticomplement activity. Other flavonoid glycosides, kaempferol 3-O-${\alpha}$-$\sub$L/-arabinopyranoside, kaempferol 3-O-${\beta}$-$\sub$D/-glucopyranoside, kaempferol 3-O-${\beta}$-$\sub$D/-galactopyranoside, quercetin 3-O-${\alpha}$-$\sub$L/-arabinopyranoside, quercetin 3-O-${\beta}$-$\sub$D/-glucopyranoside, quercetin O-${\beta}$-$\sub$D/-galactopyranoside did not showed anicomplement activity.

• Archives of Pharmacal Research
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• v.25 no.5
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• pp.652-654
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• 2002

Brine shrimp assay-guided fractionation and isolation of the EtOAc soluble fraction of Phryma leptostachya L. (Phrymacaceae) gave two active compounds, phrymarolin II (1) and ursolic acid (2), which were identified by physicochemical and spectroscopic methods. Compound 1 exhibited potent lethality with $LD_{50}$ value of 0.0013 $\mu\textrm{g}$/ml, whereas 2 showed moderate lethality with $LD_{50}$ value of 27.0 $\mu\textrm{g}$/ml against brine shrimp. The cytotoxic activities of 1 and 2 were also evaluated against one murine and five human cancer cell lines employing the sulforhodamin B (SRB) method. Compound 2 exhibited cytotoxic activity against L1210 and SK-MEL-2 cells with $ED_{50}$ values of 3.70 and 9.27 mg/ml, respectively, whereas 1 was devoid of any cytotoxic activity against all cancer cells tested.

• Archives of Pharmacal Research
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• v.27 no.6
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• pp.593-599
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• 2004

From the EtOAc fraction of the MeOH extract of Albizzia julibrissin (Leguminosae）, a rare 5-deoxyflavone (geraldone, 1）, isookanin (2）, luteolin (3）, an isoflavone (daidzein, 4）, five prenylated flavonoids ［soph6f1avescenol (5）, kurarinone (6）, kurarinol (7）, kuraridin (8） and kuraridinol (9）］, a cerebroside (soya-cerebroside I, 10）, and $(-)-syringaresinol-4-O-{\beta}-D-glucopyranoside$ (11） were isolated and characterized on the basis of spectral data. Compounds 2, 3, and 11, showed 1, 1-diphenyl-2-picrylhydrazyl radical scavenging activity.

• International Journal of Oral Biology
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• v.35 no.4
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• pp.177-184
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• 2010

In our present study, we investigated the effects of continentalic acid on Streptococcus mutans (S. mutans) biofilm. Methanol extract of Aralia continentalis (A. continentalis) was suspended in water and sequentially partitioned with CHCl3, ethyl acetate (EtOAc), and n-butanol (n-BuOH). The CHCl3 fraction showed the highest activity and an antibacterial compound against S. mutans was isolated from this preparation through various chromatography methods by bioassay guided fractionation. MS, $^1H-NMR$ and $^{13}C-NMR$ analysis showed that the active principle was continentalic acid which was confirmed to show significant inhibitory effects against S. mutans biofilm. These results may provide some scientific rationale for the traditional use these extracts for the treatment of dental diseases.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.372.1-372.1
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• 2002

AIzelin (1) and quercitrin (2) isolated from the EtOAc-soluble fraction of the leaves of Litsea japonica Jussieu (Lauraceae) showed inhibitory activity against classical pathway complement system with 50% inhibitory concentration ($IC_{50}$/) values of 112.2 and 198.2 $\mu\textrm{g}$/$m\ell$. respectively. For the structure-activity relationship of flavonoids on anti-complement system. myricitrin (3) from JUQ/ans mandshurica Maximowicz (Juglandaceae) also tested anti-complement activity. while this was devoid of any significant activity. (omitted)

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.193.3-194
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• 2003

Platelets play critical roles in both hemostasis and thrombosis. It was reported that platelet aggregation is associated with an increase in superoxide production and can be inhibited by hydroxyl radical scavengers. In the course of our search for the anti-platelet, anti-coagulant and/or anti-oxidative components from plants, the MeOH extract of Crassula cv. "Himaturi" (Crassulaceae) was observed to have both anti-aggregatory and anti-coagulant effects. A novel compound, 12-O-(4'-O-methyl-galloyl)-bergenin (1), was isolated as an active component from the EtOAC soluble fraction. (omitted)

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.194.3-194.3
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• 2003

As a part of an ongoing collaborative program to discover novel bioactive components of plant origin, the stem barks of Kalopanax septemlobus were extracted with MeOH, and successively partitioned with CH$_2$Cl$_2$, EtOAc, BuOH and water. Repeated column chromatographic separation of the CH$_2$Cl$_2$ fraction resulted in the isolation of four compounds. Their structures were identified as vladinol E (1), (-)-simulanol｛4-［3-hydroxymethyl-5-((E)-3-hydroxypropenyl)-7-methoxy-2,3-dihydrobenzofuran-2-yl］-2,6-dimethoxy-phenol｝ (2), vladinol F (3), and (${\pm}$)-secoisolariciresinol (4). This is the first report on the isolation of these compounds from Kalopanax species.

• Proceedings of the PSK Conference
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• 2003.10b
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• pp.197.4-198
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• 2003

As an attempt to search for bioactive natural constituents exerting antinociceptive and antiinflammatory activities, we examined the potency of the extract of R. coreanus fruits by the activity-guided fractionation. The EtOAc- and BuOH fraction and those alkaline hydrolysates showed significant antinociceptive effects as assessed by writhing-, hot plate- and tail flicks tests in mice and rats as well as antiinflammatory effect in rats with carrageenan-induced edema. BuOH extract was subjected to column chromatography to obtain a large amount of niga-ichigoside F$_1$ (1, 23-hydroxytormentic acid 28-O-glc), which was again hydrolyzed in NaOH solution to yield an aglycone 23-hydroxytormentic acid (1a). (omitted)