• Journal of Gastric Cancer
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• 제13권2호
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• pp.111-116
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• 2013

Purpose: Chronic inflammation induces cancer and cancer induces local tissue damage with systemic inflammation. Therefore, the aim of this study is to investigate the potential relationship between the severity of inflammation and prognosis in cancer patients. Materials and Methods: This study enrolled 220 patients from January 2002 to December 2006 who underwent gastric surgery. We evaluated the relationship between preoperative inflammatory parameters (erythrocyte sedimentation rate, neutrophil-to-lymphocyte ratio) and other clinicopathological factors. Survival outcomes were compared according to the extent of inflammation. Results: Significant elevation of erythrocyte sedimentation rate was related with old age, increased neutrophil-to-lymphocyte ratio, decreased hemoglobin, increased carcinoembryonic antigen, increased tumor size and advanced TNM stage. Neutrophil-to-lymphocyte ratio was significantly correlated with old age, increased erythrocyte sedimentation rate and advanced TNM stage. In the univariate analysis, elevated erythrocyte sedimentation rate and increased neutrophil-to-lymphocyte ratio had significantly poorer survival than those without elevation (all P<0.05). However, the multivariate analysis failed to prove erythrocyte sedimentation rate and neutrophil-tolymphocyte ratio as independent prognostic factors. Conclusions: The elevation of erythrocyte sedimentation rate and neutrophil-to-lymphocyte ratio were correlated with poor prognosis in the univariate analysis and there was a strong correlation between inflammatory parameters (erythrocyte sedimentation rate and neutrophil- to-lymphocyte ratio) and tumor progression. Thus, erythrocyte sedimentation rate and neutrophil-to-lymphocyte ratio are considered useful as follow-up factors.

• 대한방사선기술학회지:방사선기술과학
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• 제31권1호
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• pp.49-54
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• 2008

선형가속기를 이용한 X-선 5Gy를 마우스 모델에 전신 조사 후 백혈구, 적혈구, 혈소판을 주사전자현미경으로 관찰하였다. 또한 프로폴리스를 복강 주사 후 혈구들의 방사선 방어효과를 알아보았다. 혈구의 미세구조 관찰에서는 외관상 방사선 조사한 실험군에서 수가 줄어들었으며, 조사 후 10일에서 20일로 경과함에 따라 혈구의 엉김이 관찰되었다. 적혈구에서는 표면이 균열이 생기고 깨어진 형태와 변성구상적혈구들이 관찰되었다. 백혈구는 방사선조사군에서 혈구의 크기가 줄어들었으며, 가장자리 미융모들의 크기와 수가 대조군 보다 작은 것으로 관찰되었다. 프로폴리스 투여 후 방사선 조사군에서는 혈구들이 대조군과 유사한 형태로 확인되었다.

• The Korean Journal of Physiology
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• 제20권2호
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• pp.209-217
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• 1986

The functional molecular weight of band 4.5 polypeptide was measured by applying the classical target theory to radiation inactivation data of the cytochalasin B binding. Band 4.5 polypeptides purified from human erythrocyte membranes were irradiated at -45 to $-50^{\circ}C$ with an increasing dose of 1.5 MeV electron beam, and after thawing, cytochalasin B binding activities were assayed. Each activity measured was reduced as a simple exponential function of radiation dose. $D_{37}$, dose appeared to be 6.7 mega rads, from which the target size (radiation sensitive mass) of band 4.5 polypeptide was calculated to be 95,500 daltons. This result with other informations available in literature suggests that band 4.5 polypeptide may exist as a dimer in human erythrocytes.

• 한국양식학회지
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• 제13권3호
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• pp.193-196
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• 2000

버들치, Rhynchocypris oxycephalus와 버들개, R. steindachneri에서의 flowcytometry에 의한 핵 DNA 함량 측정과 세포유전학적 분석을 실시하였다. 버들치와 버들개의 핵형은 2n=50 (FN=90)으로 12개의 중부염색체, 28개의 차중부염색체 및 10개의 단부염색체로 구성되어 유사하였으나, 핵 DNA 함량은 각각 2.64 pg/nucleus 및 2.52pg/nucleus로 나타났다(P<0.05). 두 종간 적혈구 핵과 세포 크기는 유사한 반면, 버들치의 적혈구 수는 버들개의 적혈구 수보다 적었다. 본 연구 결과는 버들치속, Rhynchocypris 어류인 버들치와 버들개간 에서의 진화는 두 종간 핵형과 적혈구 크기의 명확한 변화 없이 핵 DNA 함량의 증가만이 이루어진 것을 시사한다.

• Fisheries and Aquatic Sciences
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• 제20권11호
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• pp.29.1-29.7
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• 2017

The objective of this study was to determine a simple and reliable ploidy identification protocol for the rainbow trout (RT), Oncorhynchus mykiss, in the field condition. To evaluate the ploidy level and compare different detection protocols, triploid RT and gynogenesis were induced by UV irradiation and/or heat shock. The hatching rate at day 30 was 85.2% and the survival rate at day 90 was 69.4% (fingerling). The sex ratio of female RT was 93.75% in the gynogenesis group, illustrating that the UV irradiation inactivated the sperm DNA. The hatching rate and survival rate were 82.0 and 74.7%, respectively, in the triploid-induced group. The triploid induction rate by heat shock procedure was 73.9%. Cytogenetic protocols for ploidy identification such as chromosome counting, erythrocyte nuclear size comparison, and analysis of nucleolar organizing regions (NORs) by silver staining were compared. Silver nitrate staining showed the greatest success rate (22/23 and 32/32 for the triploid-induced group and gynogenesis group, respectively), followed by erythrocyte nuclear size comparison (16/23 and 19/32 for the triploid-induced group and gynogenesis group, respectively) and, lastly, chromosome preparation (2/23 and 6/32 for the triploid-induced group and gynogenesis group, respectively) with the lowest success rate. Based on our findings, silver staining for RT ploidy identification is speculated to be highly applicable in a wide range of research conditions, due to its cost-effectiveness and simplicity compared to other numerous ploidy detection protocols.

• Applied Microscopy
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• 제18권2호
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• pp.167-176
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• 1988

Extrachromosomal circular DNA complexes from erythrocytes and splenocytes isolated from Carassius carassius were examined by mica-press-absorption method. The method was described that released small polydisperse circular DNA molecules in situ from the erythrocytes and the splenocytes and that allows selective observation of the small circular DNA complexes bound to cellular components. The released polydisperse circular DNA complexes were absorbed preferentially on mica in a divalent cation-free medium then processed for electron microscopy. Small circular DNAs showed a heterogeneous size distribution of $2{\sim}10{\mu}m$ with a mean contour length of $4.3{\mu}m$ for the circulating erythrocytes and that of $0.7{\sim}3.6{\mu}m$ with a mean contour of length $2.04{\mu}m$ for the splencytes. Cells contained $100{\sim}300$ copies and $300{\sim}700$ copies obtained from the erythrocytes and the splenocytes, repectively. Possible biological functional implications for size distribution of extrachromosomal circular DNAs are discussed.

• 한국발생생물학회지:발생과생식
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• 제19권3호
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• pp.127-134
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• 2015

The influence of triploidization on cell and nucleus size characteristics of the same tissues of erythrocyte, retina, kidney, hepatocyte and midgut epithelium in marine medaka, Oryzias dancena has been determined histologically. Induced triploid fish are produced by cold shock treatments. Likewise, the size of horizontal cell nucleus in inner nuclear layer of retina, ganglion cell nucleus in ganglion cell layer of retina, proximal tubule cell of kidney, hepatocytes and nuclear height of midgut epithelium all appear to be significantly larger than diploid (P<0.05). On the other hand, retina thickness is larger in diploid than induced triploid (P<0.05). Induced triploid shows low density of cell number. Results of this study suggest that same characteristics in the induced triploid exhibiting larger cells and nucleus sizes with fewer number of cells than the diploid can be useful criteria for the distinction between diploid and induced triploid, and also the ploidy level in marine medaka.

• 대한핵의학회지
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• 제6권1호
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• pp.33-39
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• 1972

This is an attempt to evaluate normal spleen size in the Koreans. Spleen scans were obtained in 29 patients who were admitted to the Seoul National University Hospital because of trivial illnesses. All scans were performed by use of $^{51}Cr$-labelled, heat-treated erythrocyte. As a simple screening criteria for the presence of splenomegaly, vertical height of the spleen was considered to be the best one. After statistical analysis it was concluded that the upper limit of normal spleen height is 13cm in anterior view and 14cm in lateral view.

• 한국양식학회지
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• 제21권4호
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• pp.259-264
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• 2008

한국산 누치속(genus Hemibarbus) 어류 3종에 대한 세포유전학적 특성을 구령하기 일해 적혈구 세포 크기, 세포당 DNA 함량 및 염색체 핵형을 분석하였다. 누치 H. labeo, 참마자 H. longirostris 및 어름치 H. mylodon의 적혈구 크기를 비교한 결과, 적혈구 세포 장경, 세포 표면적 및 세포 체적에서 어름치가 다른 2종보다 크게 나타났다(P<0.05). 그러나 DNA 함량은 적혈구 세포 크기와 다른 경향을 보여 2.33-2.51 범위로 3종간에 유의적인 차이를 보이지는 않았다(P>0.05). 누치속 어류 3종의 염색체 수는 모두 2n=50개로 나타났으나 핵형에 있어서는 종별로 서로 달라 누치 16M+16SM+18T,A (Fundamental number: NF, 82), 참마자 18M+16SM+16T,A (NF, 84) 그리고 어름치가 18M+24SM+8T,A (NF, 92)로 나타나 3종은 핵형에서 뚜렷한 차이를 보였다.

• 한국어류학회지
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• 제6권1호
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• pp.71-78
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• 1994

참돔 암 수 친어에 5 mg/kg 체중의 잉어 뇌하수체 추출물(CPE) 및 1,000 IU/kg 체중의 태반성 성선자극 호르몬(HCG)을 주사, 인공 산란을 유도한 후 수정난에 $3^{\circ}C$의 저온 처리를 가하여 참돔 3배체를 유도하였다. 그 결과 수정 후 6분에 $3^{\circ}C$의 저온으로 20분 및 30분간 처리시 100%의 3배체 유도율을 나타내었으며 초기 생존율을 고려시 81.3% 및 80.0%의 높은 3배체 생산율을 각각 나타내었다. 유도 3배체는 2배체에 비해 염색체수와 적혈구 세포 및 핵의 크기에 있어 증가를 보여 3배체는 2배체에 비해 세포핵의 부피가 1.58배 증가하였다. 염색체 및 핵형은 대조군이 2n=48 acrocentric 염색체인 반면, 3배체는 72개의 acrocentric 염색체로 구성되어 있었다. 부화 10일 후부터 34일 까지의 유도 3배체의 초기 전장 성장 및 부레 성장은 2배체의 그것에 비해 낮게 나타났다.