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http://dx.doi.org/10.1186/s41240-017-0074-8

Comparison of different ploidy detection methods in Oncorhynchus mykiss, the rainbow trout  

Kim, Hong Seab (Noah Biotech Inc.)
Chung, Ki-Hwa (Department of Animal Resources and Technology, Gyeongnam National University of Science and Technology)
Son, Jung-Ho (Noah Biotech Inc.)
Publication Information
Fisheries and Aquatic Sciences / v.20, no.11, 2017 , pp. 29.1-29.7 More about this Journal
Abstract
The objective of this study was to determine a simple and reliable ploidy identification protocol for the rainbow trout (RT), Oncorhynchus mykiss, in the field condition. To evaluate the ploidy level and compare different detection protocols, triploid RT and gynogenesis were induced by UV irradiation and/or heat shock. The hatching rate at day 30 was 85.2% and the survival rate at day 90 was 69.4% (fingerling). The sex ratio of female RT was 93.75% in the gynogenesis group, illustrating that the UV irradiation inactivated the sperm DNA. The hatching rate and survival rate were 82.0 and 74.7%, respectively, in the triploid-induced group. The triploid induction rate by heat shock procedure was 73.9%. Cytogenetic protocols for ploidy identification such as chromosome counting, erythrocyte nuclear size comparison, and analysis of nucleolar organizing regions (NORs) by silver staining were compared. Silver nitrate staining showed the greatest success rate (22/23 and 32/32 for the triploid-induced group and gynogenesis group, respectively), followed by erythrocyte nuclear size comparison (16/23 and 19/32 for the triploid-induced group and gynogenesis group, respectively) and, lastly, chromosome preparation (2/23 and 6/32 for the triploid-induced group and gynogenesis group, respectively) with the lowest success rate. Based on our findings, silver staining for RT ploidy identification is speculated to be highly applicable in a wide range of research conditions, due to its cost-effectiveness and simplicity compared to other numerous ploidy detection protocols.
Keywords
Rainbow trout; Nucleolar organizing regions; Silver staining; Triploid; Gynogenesis;
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1 Alcantar-Vazquez JP, Dumas S, Puente-Carreon E, Pliego-Cortes HS, Pena R. Induction of triploidy in spotted sand bass (Paralabrax maculatofasciatus Steindachner, 1868) by cold shock. Aquac Res. 2008;39:59-63.
2 Barch MJ, Knusten T, Spurbeck JL. The AGT cytogenetic laboratory manual. 3rd ed. New York: Raven; 1997. p. 25-50.
3 Cal RM, Vidal S, Gomez C, Alvarez-Blazquez B, Martinez P, Piferrer F. Growth and gonadal development in diploid and triploid turbot (Scophthalmus maximus). Aquaculture. 2006;251:99-108.   DOI
4 Carman O, Oshiro T, Takashima F. Variation in the maximum number of nucleoli in diploid and triploid common carp. Nippon Suisan Gakkai Shi. 1992;58:2303-9.   DOI
5 Caterina F, Francesca A, Giuseppe P, Alessandro Z, Francesco F. Effect of three different anticoagulants and storage time on haematological parameters of Mugil cephalus (Linneaus, 1758). Turk J Fish Aquat Sci. 2014;14:615-21.
6 Colihueque N, Ittura P, Estay F, Diaz NF. Diploid chromosome number variations and sex chromosome polymorphism in five cultured stains of rainbow trout Oncorhynchus mykiss. Aquaculture. 2001;198:63-77.   DOI
7 Deng W, Tsao SW, Lucas JN, Leung CS, Cheung ALM. A new method for improving metaphase chromosome spreading. Cytometry A. 2003;51A:46-51.   DOI
8 Felip A, Piferrer F, Zanuy S, Carrillo M. Comparative growth performance of diploid and triploid European sea bass over the first four spawning seasons. J Fish Biol. 2001;58:76-88.   DOI
9 Felip A, Zanuy S, Carrillo M, Martinez G, Ramos J, Piferrer F. Optimal conditions for the induction of triploidy in the sea bass (Dicentrarchus labrax L.). Aquaculture. 1997;152:287-98.   DOI
10 Fernandez-Diez C, Gonzalez-Rojo S, Lombo M, Herraez MP. Reproduction. 2016; 152:57-67.   DOI
11 Thogaard GH. Chromosome set manipulation and sex control in fish. In: Hoar WS, Randall DJ, Donaldson EM, editors. Fish physiology. New York: Academic; 1983. p. 405-34.
12 Piferrer F, Beaumont B, Falguiere JC, Flajshans DM, Haffray PE, Colombo L. Polyploy fish and shellfish: production, biology and applications to aquaculture for performance improvement and genetic containment. Aquaculture. 2009;293:125-56.   DOI
13 Porto-Foresti F, Oliveira C, Tabata YA, Rigolino MG, Foresti F. NORs inheritance analysis in crossings including individuals from two stocks of rainbow trout (Oncorhynchus mykiss). Hereditas. 2002;136:227-30.   DOI
14 Pradeep PJ, Srijaya TC, Jose D, Papini A, Hassan A, Chatterji AK. Identification of diploid and triploid red tilapia by using erythrocyte indices. Caryologia. 2011; 64:485-92.
15 Shimizu Y, Oshiro T, Sakaizumi M. Electrophoretic studies od diploid, triploid and tetraploid forms of the Japanese silver crucian carp, Carassius auratus langsdorfi. Jpn J Ichthyol. 1993;40:65-75.
16 Spurbeck JL, Zinsmeister AR, Meyer KJ, Jalal SM. Dynamics of chromosome spreading. Am J Med Genet. 1996;61:387-93.   DOI
17 Thomas P, Morrison R. A method to assess triploidy in swim-up rainbow trout. Austasia Aquacult. 1995;9:62-3.
18 Tiwary BK, Kirubagaran R, Ray AK. Induction of triploid by cold shock in catfish, Heteropneustes fossilis (Bloch). Asian Fish Sci. 1997;10:123-9.
19 Tiwary BK, Kirubagaran R, Ray AK. The biology of triploid fish. Rev Fish Biol Fish. 2004;14:391-402.   DOI
20 Food and Agriculture Organization (FAO). Bartley D. Fisheries and aquaculture topics. Genetic biotechnologies. Topics fact sheets. Rome: FAO Fisheries and Aquaculture Department [online]; 2005.
21 Gjedrem T, Robinson N, Rye M. The importance of selective breeding in aquaculture to meet future demands for animal protein: a review. Aquaculture. 2012;350-353:117-29.   DOI
22 Hlics R, Muhlig P, Claussen U. The spreading of metaphases is a slow process which leads to a stretching of chromosomes. Cytogenet Cell Genet. 1997;76:167-71.   DOI
23 Howell WM, Black DA. Controlled silver staining of nucleolus organizer with a protective colloidal developer: a 1-step method. Experientia. 1980;36:1014-45.   DOI
24 Hwang GD. Inland fisheries research institute yearly research report. Korea: Chungcheongbuk-do; 2012.
25 Inokuchi T, Abe S, Yamaha E, Yamazaki F, Yoshida MC. BrdU replication banding studies on the chromosomes in early embryos of salmonid fishes. Hereditas. 1994;121:255-65.
26 Jankun M, Kuzminski H, Furgala-Selezniow G. Cytologic ploidy determination in fish-an example of two salmonid species. Environ Biotechnol. 2007;3:52-6.
27 Flajshans M, Rab P, Dobosz S. Frequency analyses of active NORs in nuclei of artificially induced triploid fishes. Theor Appl Genet. 1992;85:68-72.   DOI
28 Karami A, Araghi PE, Syed MA, Wilson SP. Chromosome preparation in fish: effects of fish species and larval age. Int Aquat Res. 2015;7:201-10.   DOI
29 Kavalco KF, Pazza R. A rapid alternative technique for obtaining silver-positive patterns in chromosoms. Genet Mol Biol. 2004;27(2):196-8.   DOI
30 Kenanoglu ON, Yilmaz S, Ergun S, Aki C. A preliminary study on the determination of triploidy by chromosome analysis at the different stages of development in rainbow trout, Oncorhychus mykiss. Mar Sci Tech Bull. 2013; 2:17-21.
31 Ministry of Ocean and Fisheries. Fishery production survey, statistics by type of fishery and species (inland water fisheries). Korea; 2016. p. 205-9.
32 Alcantar-Vazquez JP. Fisiologia de los peces triploides. Lat Am J Aquat Res. 2016; 44:1-15.   DOI
33 Kligerman AD, Bloom SE. Rapid chromosome preparation from solid tissues of fishes. J Fish Res Board Can. 1977;34:266-9.   DOI
34 Lamatsch DK, Steinlein C, Schmid M, Schartl M. Non-invasive determination of genome size and ploidy level in fishes by flow cytometry detection of triploid Poecilia formosa. Cytometry. 2000;39:91-5.   DOI
35 Liu S, Sezakki D, Hashimoto K, Kobayashi H, Nakamura M. Simplified techniques for determination of polyploidy in ginbuna, Carassius auratus langsdorfi. Bull Jpn Soc Sci Fish. 1978;44:601-6.   DOI
36 Maxime V. The physiology of triploid fish: current knowledge and comparisons with diploid fish. Fish Fish. 2008;9:67-78.   DOI
37 Phillips RB, Ihssen PE. Chromosome banding in salmonid fishes: nucleolar organizer regions in Salmo and Salvelinus. Can J Genet Cytol. 1985;27:433-40.   DOI
38 Olele NF, Tiguiri OH. Optimization of triploidy induction and growth performance of Claria anguillarias (African catfish) using cold shock. Acad J Interdisc Stud. 2013;2:189-96.
39 Oliveira C, Foresti F, Rigolino MG, Tabata YA. Synaptonemal complex analysis in spermatocytes and oocytes of rainbow trout, Oncorhynchus mykiss (Pisces, Salmonidae): the process of autosome and sex chromosome synapsis. Chromosom Res. 1995;3:182-90.   DOI
40 Kizak V, Guner Y, Turel M, Kayim M. Comparison of growth performance, gonadal structure and erythrocyte size in triploid and diploid brown trout (Salmo trutta fario L., 1758). Turk J Fish Aquat Sci. 2013;13:571-80.
41 Phillips RB, Zajicek KD, Ihssen PE, Johnson O. Application of silver staining to the identification of triploid fish cells. Aquaculture. 1986;54:313-9.   DOI