• 대한의생명과학회지
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• 제15권1호
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• pp.37-45
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• 2009

Insect cell cultures have become important tools in the production of biological substances for use in a variety of research, human and veterinary medicine, and pest control applications. These applications often require the introduction of foreign DNA into the cells and have generally used methods originally developed for use with human and other mammalian cell cultures. While these methods can be successfully employed, they are often less efficient with insect cells and frequently involve complex procedures or require specialized equipment. Even when they do work, they may require substantial modification because of differences in the culture medium or growth patterns of insect cells. In this study, We have optimized transfection conditions of Sf9 cell line using insect expression vector pIZT/V5-His which expresses green fluorescent protein effectively. Human stem cell factor (hSCF) is a glycoprotein that plays a key role in hematopoiesis acting both as a positive and negative regulator, often in synergy with other cytokines. It also plays a key role in mast cell development, gametogenesis, and melanogenesis. It can exist in membrane-bound form and in proteolytically released soluble form. As determined by an enzyme-linked immunosorbent assay performed, hSCF level in supernatant averaged 995ng/ml. The human hSCF was partially purified by immunoaffinity chromatography and analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The results show that the hSCF has N-linked carbohydrate and corresponds to the soluble form, at or about 223 amino acids in length. The findings suggest functional importance for soluble hSCF in cells.

• Structural Engineering and Mechanics
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• 제53권2호
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• pp.227-248
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• 2015

A CFPBS (Cone-type Friction Pendulum Bearing System) was developed to control the acceleration delivered to a structure to prevent the damage and degradation of critical communication equipment during earthquakes. This study evaluated the isolation performance of the CFPBS by numerical analysis. The CFPBS was manufactured in the shape of a cone differenced with the existing FPS (Friction Pendulum System), and a pattern was engraved on the friction surface. The natural frequencies of the CFPBS were evaluated from a free-vibration test with a seismic isolator system consisting of 4 CFPBS. To confirm the earthquake-resistant performance, a numerical analysis program was prepared using the equation of the CFPBS induced from the equations of motion. The equation reported by Tsai for the rolling-type seismic isolation bearings was proposed to design the equation of the CFPBS. Artificial seismic waves that satisfy the maximum earthquake scale of the Korean Building Code-Structural (KBC-2005) were created and verified to review the earthquake-resistant performance of the CFPBS by numerical analysis. The superstructural mass of the CFPBS and the skew angle of friction surface were considered for numerical analysis with El Centro NS, Kobe NS and artificial seismic waves. The CFPBS isolation performance evaluation was based on the numerical analysis results, and comparative analysis was performed between the results from numerical analysis and simplified theoretical equation under the same conditions. The validity of numerical analysis was verified from the shaking table test.

• 수산해양교육연구
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• 제28권6호
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• pp.1561-1572
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• 2016

The ability to handle materials from one location to another, whether during transit or at the worksite is vital to all segments of industry. To varying degrees, many personnel in numerous workplaces take part in materials handling. Consequently, some employees are injured. In fact, rigging & slinging is a dangerous work using a crane and sling equipment to carry a cargo and the mishandling of materials is the single largest cause of accidents and injuries in the workplace. The majority of accidents associated with cranes and other lifting appliances are caused by faulty slinging, overloading, unbalanced loads, etc. which result in the load falling or tipping out of control, causing injury to people, damage to plant, machinery and the load. Therefore, recognizing the dangers of the works, there are much technical support including skill training in various institutes to minimize accidents during works on land. Although rigging work at sea is much dangerous than on land work because it needs to take account of the movements of the ships and waves, etc. in addition to land based rigging hazards, it is insufficient in appropriate actions that can improve the safety of the workers at sea. Therefore, this study suggested a rigging and slinging course for seafarers to improve their safety at sea by researching hazards and risk of rigging works and related skill training conducted on land.

• Journal of Ship and Ocean Technology
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• 제6권2호
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• pp.23-36
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• 2002

This paper describes the powering, seakeeping and maneuvering performances for a 2,500-ton class trimaran. Influence of the side-hull forms and location of those in longitudinal and transverse direction to resistance performance was systematically investigated by a series of model tests and numerical calculations. It was found that the longitudinal location of side-hulls was the most influential design parameter to the resistance performance of the trimaran and the optimum location of side-hull depends on ship speeds. When the side-hull stem is located near the primary wave hollow generated by the main hull, the trimaran shows the best resistance performance. Powering performance of the trimaran is superior to those of similar mono-hull ships. Seakeeping model tests for the trimaran were executed and the results were compared with the theoretical results of a similar mono-hull ship. Generally speaking, seakeeping performance of the trimaran is superior to that of a mono-hull ship. In particular, pitching and rolling performance of the trimaran is excellent, which is due to the increased length and breadth. Maneuvering model tests using a HPMM equipment were executed to evaluate the maneuvering performance of the trimaran. Maneuvering simulation was performed using the maneuvering coefficients from the model tests. The results show that the control ability of heading angle and the direction keeping stability of the trimaran is excellent, even though the turning performance is rather worse compared to those of a similar mono-hull ship.

• 한국반도체및디스플레이장비학회:학술대회논문집
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• 한국반도체및디스플레이장비학회 2002년도 추계학술대회 발표 논문집
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• pp.100-111
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• 2002

The effects of intake mixture temperature on performance and exhaust emissions under four kinds of engine loads were experimentally investigated by using a four-cycle, four-cylinder, swirl chamber type, water-cooled diesel engine with scrubber EGR system operating at three kinds of engine speeds. The purpose of this study is to develop the scrubber exhaust gas recirculation(EGR) control system for reducing $NO_x$ and soot emissions simultaneously in diesel engines. The EGR system is used to reduce $NO_x$ emissions. And a novel diesel soot-removal device of cylinder-type scrubber with five water injection nozzles is specially designed and manufactured to reduce soot contents in the recirculated exhaust gas to the intake system of the engine. The influences of cooled EGR and water injection, however, would be included within those of scrubber EGR system. In order to survey the effect of intake mixture temperature on performance and exhaust emissions, the intake mixtures of fresh air and recirculated exhaust gas are heated by a heating device with five heating coils made of a steel drum. It is found that the specific fuel consumption rate is considerably elevated by the increase of intake mixture temperature, and that $NO_x$ emissions are markedly decreased as EGR rates are increased and intake mixture temperature is dropped, while soot emissions are increased with increasing EGR rates and intake mixture temperature. Thus one can conclude that the performance and exhaust emissions are considerably influenced by the cooled EGR.

• 대한전자공학회논문지TE
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• 제39권2호
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• pp.95-103
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• 2002

지난 걸프전에서 보아 익히 알 수 있듯이 최근 전장에 배치되는 무기체계들은 고도로 복잡 다양해지고 있으며, 모든 무기체계들은 전자${\cdot}$정보화되어 모든 장비들이 네트워크로 연결되고 연동이 가능하게 설계되는 추세이다. 특히 구축함이나 호위함, 초계함, 잠수함 등과 같은 군함에 탑재되는 모든 함상 장비들은 컴뱃 시스템 데이터 버스(Ethernet)와 같은 네트워크로 연결되어 컴뱃 시스템에 의해 통제 운영되고 있다. 이러한 관점에서 볼 때 군함 네트워크의 정상 동작 상태 유지는 전장에서 가장 중요한 요소라 할 수 있다. 따라서, 본 논문에서는 함정의 탑재 장비간 네트워크 관리방안에 대해 제시하고, 특히 SNMP 기반 POP 폴링 기법의 구축함(DX : Destroyer, Experimental) 탑재 장비 네트워크 관리 시스템 구조설계안을 제시하며, 시뮬레이션을 통해 사용율의 변화를 측정한다.

• 대한전자공학회논문지SD
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• 제46권6호
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• pp.31-38
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• 2009

휴대용 기기의 소비 전력을 낮추고 영상의 질을 개선하기 위해, 주변 밝기에 따라서 LCD 모듈의 백라이트를 조정하는 방법을 사용할 수 있다. 이를 효과적으로 구현하기 위해서 LCD 패널에 광 센서와 신호취득 회로를 집적하고자 했으며, LTPS TFT 공정을 이용하여 설계했다. 서로 다른 LCD 패널의 광 센서에 대한 특성 편차를 보정하기 위해 새로운 개념의 start-up 보정 방식을 제안하였다. 이와 더불어 광 전류 정보를 디지털 형태로 전달하기 위해 time-to-digital 방식을 사용하였으며, 이를 start-up 보정 방식과 효과적으로 결합하는 dual slope 보정 방법을 제안하였다. LTPS TFT 공정을 이용하여 최종적인 신호취득 회로를 구현하고자, 간단하고 안정적인 회로 구조와 타이밍을 제안하고 설계 및 검증을 진행했다. 설계한 신호취득 회로는 별도의 검사 설비 없이 광 센서 편차의 보정이 가능하며, 60dB 범위의 입력 광에 대해 10배수 구간 마다 4 단계의 디지털 데이터를 출력한다. 신호취득 속도는 100Hz이며, 디지털 변환의 선형 오차는 18% 미만이다.

• 전기전자학회논문지
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• 제14권2호
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• pp.58-63
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• 2010

본 논문에서, 평탄한 경로 뿐 아니라 바위 등의 험로환경에서도 로봇이 주행하도록 다족류 절지동물과 유사한 구조를 갖는 다중 모듈형 로봇의 설계 및 구현을 다룬다. 각 모듈은 독립 전원과 제어장치, 구동부 및 근거리 Zigbee 무선통신을 포함하고 있으며 절지동물의 한마디를 구현하였다. 주행방향 및 경로 조건에 따라 각 모듈은 무선통신에 의하여 동일한 주행방향을 가지며 모듈의 구동부를 가동 또는 차단할 수 있도록 설계하였다. 주행시 경로의 조건에 따라 각 모듈의 속도와 토크를 계산하며 험한 경로의 경사도에 따라 구동 되는 모듈 수를 가변적으로 조정하여 험로 및 평탄도로 등의 다양한 경로환경에 효율적으로 주행하도록 하였다. 험로 모델을 이용한 실험을 통하여 모듈을 가변적으로 구동할 수 있는 로봇을 구현하였다.

• 전기전자학회논문지
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• 제20권3호
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• pp.310-313
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• 2016

본 논문에서는 48개 채널의 GM Tube 센서 테스터 장치를 설계한다. 제안하는 장치는 48 채널의 GM Tube 센서들을 동시에 테스트하여 불량의 여부 및 센서 특성을 분석한다. 시간의 변화에 따라 달라지는 센서의 특성에 적합한 300 ~ 1000V의 가변 고전압을 발생할 수 있는 회로로 센서의 특성을 분석한다. 따라서 다양한 종류의 GM Tube 센서의 특성 분석에 용이하게 사용된다. 제안하는 장치를 통해 대량의 GM Tube를 동시에 테스트 할 수 있는 환경이 구축되어 센서의 불량 여부 및 센서의 특성을 미리 파악하여 생산 및 재작업 등에 소요되는 비용을 대폭 줄일 수 있다. 개발된 48 채널 GM Tube 센서 테스터 장치의 측정 불확도에 대하여 공인 시험기관의 장비를 사용하여 실험한 결과 우수한 성능을 나타내었다.

• 한국결정학회지
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• 제7권2호
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• pp.147-155
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• 1996

할로겐 램프를 열원으로 이용하는 결정성장장치인 부유대역융로를 제작하여 고온초전도체 Bi2Sr2CaCu2O8의 성장에 이용하였다. 제작된 결정성장장치는 할로겐 램프를 초점에 위치시키기 위한 holder unit, 1kW의 할로겐 램프로서 최대 1800℃까지 사용이 가능한 image furnace, 냉각장치, 원료 물질을 공급하는 feeding unit, 성장된 단결정을 인상하는 pulling unit, 그리고 2mm/hr - 40mm/hr의 속도로 상하 이동 및 15rpm -120rpm의 회전이 가능한 제어부 등으로 구성되었다. 300W의 할로겐 램프를 이용하여 Bi2Sr2CaCu2O8 fiber를 성장시켰으며 성장된 fiber는 XRD, SEM, EDS 등으로 분석하였다. 성장조건은 공기분위기에서 성장속도 3∼4mm/hr, 상·하부축 회전속도 20∼25 rpm이었으며 성장된 fiber는 Bi2Sr2CaCu2O8 기지 내에 (Sr,Ca)CuO2 및 (Sr,Ca)2CuO3의 2차상을 포함하고 있었다.