• 한국임상수의학회지
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• 제20권3호
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• pp.294-301
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• 2003

개의 전층피부 창상에서 삼출물의 정도와 창상수축, 상피화, 그리고 치유에 대해 hydrocollodi(Duoderm/$\circledR$, HC)과 hydrogel(Nu-Gel$\circledR$, HG) occlusive dressing materials 의 효과를 비교하였다. 3쌍의 정사각형 전층피부 창상을 2${\times}$2cm 의 크기로 12마리 개의 몸통 등외측 면에(6개의 창상/한 마리) 만들었다. HC와 HG, 생리 식염수(대조창)를 무작위로 배분한 2개의 창상에 각각 적용하였다. 창상의 육안적 소견과 병리조직학적 소견을 4주 동안 평가하였다. 창상유발 7일 후, 비교 그룹 간의 평균 창상수축 정도, 평균 상피화율, 평균 창상 치유율에 유의성있는 변화가 관찰되지 않았다. 창상 유발 14일(P<0.01)과 21일(P<0.05) 후, HG 처치창의 평균 상피화율이 HC 처치창의 평균 상피화율과 대조창의 상피화율보다 유의적으로 더 높게 나타났다. 창상 유발 21일 후(P<0.05), HG 처치창의 창상 수축율이 HC 처치창의 평균 창상 수축율과 대조창의 평균 창상 수축율보다 유의적으로 더 높게 나타났다. 창상 유발 21일 후(P<0.02), HG 처치창의 창상 치유율이 HC 처치창의 평균 창상 치유율과 대조창의 평균 창상 치유율보다 유의적으로 더 높게 나타났다. 창상 유발 1, 4, 7일 후, HC 처치창과 대조창의 피하에 다형핵 백혈구의 심한 침착이 관찰되었고, HG 처치창의 피하에 중등도의 다형핵 백혈구의 침착이 관찰되었으나, HG 처치창, HC 처치창 간의 어떠한 유의적인 변화도 발견되지 않았다. 창상 유발 14일 후, HG 처치창에서 상피성 세포가 유의적으로 표면 위로 자라났고, 부종은 창상 밑 조직까지 감소하였으며, 육아조직은 교원질 섬유로 대체되었다. 창상 유발 21일 후, 다른 창상에 비해, HG 처치창은 유의적으로 창상 표면이 거의 재생성 상피로 덮였으며, 육아조직은 창상 유발 14일 후와 비교해서 유의적으로 교원질 섬유로 대체되었다. 위의 모든 결과에서 보듯이, 개에서 전층피부 창상의 처치 시 HG의 사용은 HC와 생리 식염수에 비해 창상치유 복구기의 치유 속도를 촉진하는 것으로 사료된다.

• Archives of Plastic Surgery
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• 제41권6호
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• pp.638-646
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• 2014

Background The combination of polycaprolactone and hyaluronic acid creates an ideal environment for wound healing. Hyaluronic acid maintains a moist wound environment and accelerates the in-growth of granulation tissue. Polycaprolactone has excellent mechanical strength, limits inflammation and is biocompatible. This study evaluates the safety and efficacy of bio-conjugated polycaprolactone membranes (BPM) as a wound dressing. Methods 16 New Zealand white rabbits were sedated and local anaesthesia was administered. Two $3.0{\times}3.0cm$ full-thickness wounds were created on the dorsum of each rabbit, between the lowest rib and the pelvic bone. The wounds were dressed with either BPM (n=12) or Mepitel (n=12) (control), a polyamide-silicon wound dressing. These were evaluated macroscopically on the 7th, 14th, 21st, and 28th postoperative days for granulation, re-epithelialization, infection, and wound size, and histologically for epidermal and dermal regeneration. Results Both groups showed a comparable extent of granulation and re-epithelialization. No signs of infection were observed. There was no significant difference (P>0.05) in wound size between the two groups. BPM (n=6): $8.33cm^2$, $4.90cm^2$, $3.12cm^2$, $1.84cm^2$; Mepitel (n=6): $10.29cm^2$, $5.53cm^2$, $3.63cm^2$, $2.02cm^2$; at the 7th, 14th, 21st, and 28th postoperative days. The extents of epidermal and dermal regeneration were comparable between the two groups. Conclusions BPM is comparable to Mepitel as a safe and efficacious wound dressing.

• Journal of Chest Surgery
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• 제30권8호
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• pp.739-746
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• 1997

기관결손을 대체할 이장적인 보철물을 개발하려는 다양한 실험연구가 있었다. 인공기관보철물의 조건으로 는 공기에 비투과성, 적이지 않는 형태의 안전성, 생체내에서 섬 유아세포의 증식과 상피세포 재생에 필요한 생체적합성이 요구된다. 생체적합성이 우수한 포리우레탄 소재의 다공성포리우레탄 튜브에 gelatin으로 도포하고 isoplastic고리를 보강하여 외경 20 m길이 30 m의 인공기관보철물을 제작하였다. 인공기관의 생체적합성과 상피세포재생을 촉진하기위해 콜라겐 물질인 gelatin을 감마선으로 고정하여 도포하였다. 인공기관보철물을 잡종성견 10마리를 대상으로 경흉부기관에 이식하였다. 실험군에서는 다공성 폴리머에 gelatin으로 피복한 인공기관을 이식하였고 대조군에서는 gelatin으로 피복하지않은 인공기관과 ,다공성 폴리머에 gelatin을 피복한 기관에 자가심장 편을 접합시킨 것을 이식하여 실험하였다. 다공성 폴리머에 gelatin피복한 기관과 자가심낭편을 접합한 기관 을 이식한 후 6주째에 인공기관 내면에 상피화가 관찰되었다. gelatin 피복한 기관을 대상으로 외번문합과 내 문합을 비교한 결과 외번문합에서 문합부의 육아종 협착이 더 심하게 생겼다. 심낭편을 접착한 기관에서는 문합부와 접착부에 감염과 염증이 있었다. 본 실험결과 다공성포리우레탄 튜브에 gelatin을 도포한 인공기관 보철물은 상피재생과 생체적합성이 우수하며 재질개선과 문합부의 협착을 막는 연구보완이 되면 임상적용 을 기대 할수 있다.

• Fibers and Polymers
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• 제6권3호
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• pp.219-223
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• 2005

Water-soluble chitin (WSC) was prepared by carefully deacetylating chitins to about $50\%$ of N-acetyl content. Topical formulations based on WSC were prepared and their effects on wound healing were evaluated on a rabbit ear model. Full-thickness, open skin wounds were made on the ears of rabbits and WSC ointments were embedded in the open wounds. The application of WSC ointments significantly accelerated wound healing and wound contraction. The areas of epithelial-ization and granulation tissues in WSC ointment group are remarkably larger than those in control group (no treatment) and in placebo group (treated with ointment-base materials). A large number of grown granulation tissues including dense fibroblast deposition were observed under the thickened epithelium of the wound treated with WSC ointments. The number of inflammatory cells in WSC ointment group was significantly decreased compared with those in control and placebo groups, indicating that WSC would give low stimuli to wounds and prevent excessive scar formation. Neovascularization was the most prominent in WSC ointment group. Wound contraction in WSC ointment group was much larger than those in control and placebo groups. Overall results demonstrate that the topical formulation based on WSC is considered to become an excellent dressing as a wound healing assistant.

• Journal of Pharmaceutical Investigation
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• 제32권2호
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• pp.113-117
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• 2002

Cyto-toxic effect of silver sulfadiazine (Ag-SD) on keratinocytes and its implication on wound healing process were investigated in $2^{nd}$ degree bum hairless mouse model. As a dermal model, HaCat (immortalized keratinocytes) monolayer culture in DMEM with 10% FBS was used. Cyto-toxicity of Ag-SD was estimated by measuring the cell viability using neutral red assay after adding the drug. The $2^{nd}$ degree bum was prepared on hairless mouse back skin (1 cm diameter) and dressings with Ag-SD were applied for 96 hr. The process of re-epithelialization and the presence of inflammatory cells were investigated and histology with Hematoxylin-Eosin staining was performed. Ag-SD displayed highly cyto-toxic effect on cultured HaCat cells in a concentration dependent manner $(1-100\;{\mu}g/mL)$. Topical application of Ag-SD (2%) could control the infection: no inflammatory cells were observed in histology. However the cyto-toxic effect of Ag-SD on skin cells induced the impairment in epidermal regeneration.

• 한국임상수의학회지
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• 제35권5호
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• pp.229-232
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• 2018

A 9-year-old intact female Poodle (weighing 3.6 kg) was presented for the treatment of a partial and full thickness burn that covering 45% of body including bilateral thigh and sacrolumbar region. Autologous platelet-rich plasma (PRP) stimulates angiogenesis, promoting vascular in-growth and fibroblast proliferation. On the unrecovered right thigh of the dog, autologous PRP was injected into the lesion after application of micro-needles. For macroscopic evaluation digital, photographs were taken from wounds at days 0, 3, 5, 13, 21, and 28. On the $3^{rd}$ day after PRP application, epithelialization was accelerated. Application of autologous PRP accelerated wound-healing rate and healing time in full thickness burns as well as secondary complications originating from unrecovered wounds. The delayed lesion was completely healed on the $28^{th}$ day by autologous PRP treatment. In human, PRP was increasingly used in the treatment of a variety of soft tissue in the management of chronic non-healing wounds. This study has shown that PRP treatment can be a valuable and effective aid on intractable wound healing in the dog with burns.

• Applied Microscopy
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• 제45권2호
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• pp.80-88
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• 2015

The structural change and distribution of mitochondrial enzyme (ATPase, cytochrome-c-oxidase), cell proliferation (proliferating cell nuclear antigen, PCNA), cell death (caspase-3) and cell growth factor (fibroblast growth factor 8, FGF-8) in the Sprague-Dawley rat bile duct during Clonorchis sinensis infection was investigated. Experimental groups were divided into C. sinensis infection, superinfection and reinfection of C. sinensis after 'praziquantel' treatment group. As a result, C. sinensis infected rat bile ducts showed the features of chronic clonorchiasis, i.e., connective tissue thickening, ductal fibrosis and epithelial tissue dilatation. PCNA for cell proliferation increased in the infection group, and decreased after praziquantel treatment. Caspase-3 was distributed in reinfection group only. FGF-8 was distributed in the rat bile duct after praziquantel treatment but not distributed in infection and reinfection group. Overall, C. sinensis infection causes physical and chemical irritations and then brings on the abnormalities of intracellular energy metabolism and cellular growth factors, which hinders bile duct tissue from functioning properly, and resultingly, fibrosis occurs and epithelial cells dilated abnormally. More intense infection makes tissue fibrosis chronical and activates apoptosis factors.

• 한국임상수의학회지
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• 제11권1호
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• pp.427-436
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• 1994

To investigate the effect of yeast glucan on wound healing in rabbits, histopathological findings and wound contraction after topical application of glucan on the open wound were observed. Formalin fixation and embedding of tissues at wound were prepared for histopathological findings at the other day after wounding. Wound size was measured for contraction. Macrophages appeared in the deeper site under the scab at day 1 after wounding in the glucan treated group, but not in the control. A number of macrophages, fibroblasts and a small number of neutrophils were observed at day 3 after wounding In the glucan treated group as compared with the control. Histopathological findings at day 5 after wounding in control were similar with those at day 3 in the glucan treated group. Glucan like granules were scattered in the cytoplasm of macrophages at day 5 in the glucan treated group. A great portion of wound reepithelialized at day 5 in the glucan treated group but a small portion of wound in the control. The Present study indicated that the treatment of 5,000$\mu\textrm{g}$/$m\ell$ of glucan has an effect on wound heal. ins in respect to migration of macrophage into the wound site, granulation tissue formation and epithelialization.

• Journal of Trauma and Injury
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• 제22권2호
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• pp.227-232
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• 2009

Purpose: Numerous experimental studies have shown the benefits of treating thermal burns by cooling. Nevertheless, few studies have shown the clinical effect of cooling therapy on thermal burns. This study aimed to identify the clinical effect of immediate cooling therapy. Methods: The research was conducted as a retrospective, case-control study. All patients had thermal injuries characterized as a superficial second-degree burn. In the cooling group, 14 patients had first-aid cooling therapy delivered by either parents, caregivers, general practitioners, local hospitals, and/or Myongji hospital. Included in the study were 22 control patients who were not treated with any cooling therapies. Other clinical factors, such as age, sex, cause of burn injury, and burn area (Total Body Surface Area %), were taken into consideration. The duration of treatment was defined as the time from the occurrence of the injury to the presence of complete re-epithelialization, as confirmed by two surgeons. Results: The duration of treatment in the cooling group was significantly less than that the control group (p<0.05). Conclusion: Cooling therapy as an initial emergent treatment is clinically effective for superficial second-degree burn injuries.

• 생약학회지
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• 제46권1호
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• pp.59-64
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• 2015

Wound healing is a complex process that includes inflammation, granulation tissue formation, re-epithelialization, and remodeling. We reported previously that BuOH fraction from Hydnocarpi Semen (HS) crude extract exhibited wound healing activity in animal ulcer models. In this study, we investigated whether BuOH fraction activates keratinocyte and fibroblast via wound closure test and migration assay. In the scratch test, BuOH fraction accelerated the closure of a monolayer wound scratch at $100{\mu}g/mL$. After treatment with BuOH fraction for 18 h, keratinocytes showed a increase in migration at $25{\mu}g/mL$, whereas the migration of fibroblast increased significantly at $100{\mu}g/mL$ of BuOH fraction compared to control. The mechanism that the BuOH fraction of HS helps to promote healing by inflammation is possibly associated with the migration of keratinocyte and fibroblast.