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Clinical Application of Autologous Platelet-Rich Plasma (PRP) on Delayed Wound Healing of a Dog with Burns

  • Lee, Shinho (Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University) ;
  • Cheong, Jongtae (Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University) ;
  • Lee, Joo-Myoung (Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University)
  • Received : 2018.08.17
  • Accepted : 2018.10.05
  • Published : 2018.10.31

Abstract

A 9-year-old intact female Poodle (weighing 3.6 kg) was presented for the treatment of a partial and full thickness burn that covering 45% of body including bilateral thigh and sacrolumbar region. Autologous platelet-rich plasma (PRP) stimulates angiogenesis, promoting vascular in-growth and fibroblast proliferation. On the unrecovered right thigh of the dog, autologous PRP was injected into the lesion after application of micro-needles. For macroscopic evaluation digital, photographs were taken from wounds at days 0, 3, 5, 13, 21, and 28. On the $3^{rd}$ day after PRP application, epithelialization was accelerated. Application of autologous PRP accelerated wound-healing rate and healing time in full thickness burns as well as secondary complications originating from unrecovered wounds. The delayed lesion was completely healed on the $28^{th}$ day by autologous PRP treatment. In human, PRP was increasingly used in the treatment of a variety of soft tissue in the management of chronic non-healing wounds. This study has shown that PRP treatment can be a valuable and effective aid on intractable wound healing in the dog with burns.

Keywords

References

  1. Abegao KG, Bracale BN, Delfim IG, Santos ES, Laposy CB, Nai GA, Nogueir, RM. Effects of heterologous platelet-rich plasma gel on standardized dermal wound healing in rabbits. Acta Cir Bras, 2015; 30: 209-215. https://doi.org/10.1590/S0102-865020150030000008
  2. Abuaf OK, Yildiz H, Baloglu H, Bilgili ME, Simsek HA, Dogan B. Histologic Evidence of New Collagen Formulation Using Platelet Rich Plasma in Skin Rejuvenation: A Prospective Controlled Clinical Study. Ann Dermatol 2016; 28: 718-724. https://doi.org/10.5021/ad.2016.28.6.718
  3. Araki J, Jona M, Eto H, Aoi N, Kato H, Suga H, Yoshimura K. Optimized preparation method of platelet-concentrated plasma and noncoagulating platelet-derived factor concentrates: maximization of platelet concentration and removal of fibrinogen. Tissue Eng Part C Methods 2012; 18: 176-185.
  4. Bohling MW. Burns. In: Veterinary Surgery Small Animal. Tobias KM, Johnston SA (eds). Elsevier: St Louis, 2014; 1291-1302.
  5. Calderon Mdel S, Figueroa CS, Arias JS, Sandoval AH, Torre FO. Combined therapy of Ulmo honey (Eucryphia cordifolia) and ascorbic acid to treat venous ulcers. Rev Lat Am Enfermagem 2015; 23: 259-266. https://doi.org/10.1590/0104-1169.0020.2550
  6. Canapp SO, Jr. Leasure CS, Cox C, Ibrahim V, Carr BJ. Partial Cranial Cruciate Ligament Tears Treated with Stem Cell and Platelet-Rich Plasma Combination Therapy in 36 Dogs: A Retrospective Study. Front Vet Sci 2016; 3: 112.
  7. Carter CA, Jolly DG, Worden CE, Sr. Hendren DG, Kane CJ. Platelet-rich plasma gel promotes differentiation and regeneration during equine wound healing. Exp Mol Pathol 2006; 74: 244-255.
  8. Crovetti G, Martinelli G, Issi M, Barone M, Guizzardi M, Campanati B, Carabelli A. Platelet gel for healing cutaneous chronic wounds. Transfus Apher Sci 2004; 30: 145-151. https://doi.org/10.1016/j.transci.2004.01.004
  9. DeSanti L. Pathophysiology and current management of burn injury. Adv Skin Wound Care 2005; 18: 323-332. https://doi.org/10.1097/00129334-200507000-00013
  10. Fernandez-Moure JS, Van Eps JL, Cabrera FJ, Barbosa Z, Medrano Del Rosal G, Weiner BK, Tasciotti E. Platelet-rich plasma: a biomimetic approach to enhancement of surgical wound healing. J Surg Res 2017; 207: 33-44. https://doi.org/10.1016/j.jss.2016.08.063
  11. Franceschi F, Papalia R, Franceschetti E, Paciotti M, Maffulli N, Denaro V. Platelet-rich plasma injections for chronic plantar fasciopathy: a systematic review. Br Med Bull 2014; 112: 83-95. https://doi.org/10.1093/bmb/ldu025
  12. Hatakeyama I, Marukawa E, Takahashi Y, Omura K. Effects of platelet-poor plasma, platelet-rich plasma, and plateletrich fibrin on healing of extraction sockets with buccal dehiscence in dogs. Tissue Eng Part A 2014; 20: 874-882.
  13. Hermeto LC, Rossi Rd Padua SB, Pontes ER, Santana AE. Comparative study between fibrin glue and platelet rich plasma in dogs skin grafts. Acta Cir Bras 2012; 27: 789-794. https://doi.org/10.1590/S0102-86502012001100008
  14. Kakudo N, Kushida S, Minakata T, Suzuki K, Kusumoto K. Platelet-rich plasma promotes epithelialization and angiogenesis in a split thickness skin graft donor site. Med Mol Morphol 2011; 44: 233-236. https://doi.org/10.1007/s00795-010-0532-1
  15. Kazakos K, Lyras DN, Verettas D, Tilkeridis K, Tryfonidis M. The use of autologous PRP gel as an aid in the management of acute trauma wounds. Injury 2009; 40: 801-805. https://doi.org/10.1016/j.injury.2008.05.002
  16. Kim YC, Park JH, Prausnitz MR. Microneedles for drug and vaccine delivery. Adv Drug Deliv Rev 2012; 64: 1547-1568. https://doi.org/10.1016/j.addr.2012.04.005
  17. Lee JH, Kim MJ, Ha SW, Kim HK. Autologous Plateletrich Plasma Eye Drops in the Treatment of Recurrent Corneal Erosions. Korean J Ophthalmol 2016; 30: 101-107. https://doi.org/10.3341/kjo.2016.30.2.101
  18. Liu M, Lu J, Muller P, Turnbull L, Burke CM, Schlothauer RC, Harry EJ. Antibiotic-specific differences in the response of Staphylococcus aureus to treatment with antimicrobials combined with manuka honey. Front Microbiol 2014; 5: 779.
  19. Lu Y. Effects of "surrounding needling" on hydroxyproline content and ultrastructures in the dermis of aged rats. Zhongguo Zhen Jiu 2008; 28: 61-64.
  20. Marquez-de-Aracena R, Montero-de-Espinosa I, Munoz M, Pereira G. Subconjunctival application of plasma platelet concentrate in the treatment of ocular burns. Preliminary results. Arch Soc Esp Oftalmol 2007; 82: 475-481.
  21. Monteiro SO, Lepage OM, Theoret CL. Effects of plateletrich plasma on the repair of wounds on the distal aspect of the forelimb in horses. Am J Vet Res 2009; 70: 277-282. https://doi.org/10.2460/ajvr.70.2.277
  22. Nofal E, Helmy A, Nofal A, Alakad R, Nasr M. Platelet-rich plasma versus CROSS technique with 100% trichloroacetic acid versus combined skin needling and platelet rich plasma in the treatment of atrophic acne scars: a comparative study. Dermatol Surg 2014; 40: 864-873.
  23. Pallua N, Wolter T, Markowicz M. Platelet-rich plasma in burns. Burns 2010; 36: 4-8. https://doi.org/10.1016/j.burns.2009.05.002
  24. Quist EM, Tanabe M, Mansell JE, Edwards JL. A case series of thermal scald injuries in dogs exposed to hot water from garden hoses (garden hose scalding syndrome). Vet Dermatol 2012; 23: 162-166 https://doi.org/10.1111/j.1365-3164.2011.01015.x
  25. Safdar A, Shaaban H, Tibayan R, Miller R, Boairdo R, Guron G. The clinical efficacy of using autologous platelet rich plasma in hip arthroplasty: A retrospective comparative study. J Nat Sci Biol Med 2015; 6: 49-55.
  26. Salemi S, Rinaldi C, Manna F, Guarneri GF, Parodi PC. Reconstruction of lower leg skin ulcer with autologous adipose tissue and platelet-rich plasma. J Plast Reconstr Aesthet Surg 2008; 61: 1565-1567. https://doi.org/10.1016/j.bjps.2008.04.048
  27. Tang, Y. Q., Yeaman, M. R., & Selsted, M. E. (2002). Antimicrobial peptides from human platelets. Infect Immun, 70: 6524-6533. https://doi.org/10.1128/IAI.70.12.6524-6533.2002
  28. Tiwari VK. Burn wound: How it differs from other wounds? Indian J Plast Surg 2012; 45: 364-373. https://doi.org/10.4103/0970-0358.101319
  29. Tozum TF, Demiralp B. Platelet-rich plasma: a promising innovation in dentistry. J Can Dent Assoc 2003; 69: 664.
  30. Zhang J, Middleton KK, Fu FH, Im HJ, Wang JH. HGF mediates the anti-inflammatory effects of PRP on injured tendons. PLoS One 2013; 8.