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The Effect of the Butanol Fraction from Hydnocarpi Semen Extract on Activation of Keratinocyte and Fibroblast  

Lee, Geum Seon (College of Pharmacy, Sahmyook University)
Lee, Ki Man (College of Pharmacy, Sahmyook University)
Yim, Dongsool (College of Pharmacy, Sahmyook University)
Cheong, Jae Hoon (College of Pharmacy, Sahmyook University)
Kang, Tae Jin (College of Pharmacy, Sahmyook University)
Publication Information
Korean Journal of Pharmacognosy / v.46, no.1, 2015 , pp. 59-64 More about this Journal
Abstract
Wound healing is a complex process that includes inflammation, granulation tissue formation, re-epithelialization, and remodeling. We reported previously that BuOH fraction from Hydnocarpi Semen (HS) crude extract exhibited wound healing activity in animal ulcer models. In this study, we investigated whether BuOH fraction activates keratinocyte and fibroblast via wound closure test and migration assay. In the scratch test, BuOH fraction accelerated the closure of a monolayer wound scratch at $100{\mu}g/mL$. After treatment with BuOH fraction for 18 h, keratinocytes showed a increase in migration at $25{\mu}g/mL$, whereas the migration of fibroblast increased significantly at $100{\mu}g/mL$ of BuOH fraction compared to control. The mechanism that the BuOH fraction of HS helps to promote healing by inflammation is possibly associated with the migration of keratinocyte and fibroblast.
Keywords
Hydnocarpi Semen; Butanol fraction; Keratinocyte; Fibroblast; Migration;
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Times Cited By KSCI : 4  (Citation Analysis)
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