• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.28 no.1
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• pp.68-84
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• 2015

Object : The object of this study was effected of composed of 4 herbal medicines(Rubus coreanus, Rehmanniae Radix, Houttuynia cordata, Betulae cortex) on acute atopic dermatitis mice. Methods : BALB/c mice were divided 4 groups ; normal group, negative control group of acute atopic dermatitis mice induced by DNCB, treated apply to the back skin with the herbal medicine group, and treated orally with herbal medicine group. Treated with herbal medicine and DNCB during 2 weeks. After treated, measured WBCs, RBCs, and platelet in the blood, and analysed mRNA expression of spleen. Result : Composed herbal medicines could reduce WBCs, lymphocytes, monocytes, neutrophils, and eosinophils in the mouse blood. And, could down TNF-${\alpha}$ levels in spleen. In the skin tissue histology results, herbal medicines reduce the cells and T cells. Conclusion : Composed herbal medicines help to another inflammatory disease because they reduce the cells and T cells in the skin tissues. And they have inhibitory effects of TNF-${\alpha}$ mRNA expression, and Th 2 immune responses. Therefore herbal medicines use for an acute atopic dermatitis treatment.

• The Korean Journal of Cytopathology
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• v.10 no.2
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• pp.185-189
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• 1999

The diagnosis of peripheral T cell lymphoma is difficult due to the varying size and shape of the neoplastic lymphoid cells and the frequent admixture of nonneoplastic mature lymphyocytes, histiocytes, eosinophils, and plasma cells. We report a case of peripheral T cell lymphoma, lymphoepithelioid ceil type, which was difficult to differentiate from tuberculous lymphadenitis due to the aggregates of epithelioid histlocytes mimicking granuloma and the past history of pulmonary tuberculosis. Fine needle aspiration cytology of the inguinal lymph node in a 63-year-old male was characterized by hypercellular aspirates composed of a mixture of small and intermediate-size lymphoid cells and large lymphoid cells with background of confluent epithelioid histiocytes. The neoplastic lymphocytes demonstrated significant nuclear irregularity with protrusion and indentations of the nuclear membrane, prominent nucleoli, and frequent mitotic figures. The diagnosis of peripheral T cell lymphoma was confirmed by histological and immunohistochemical studios.

• Toxicological Research
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• v.24 no.3
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• pp.213-218
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• 2008

In this research, the genotoxic effects of Kong-Jin-Dan(KJD), a polyherbal formula were evaluated using the mouse micronucleus test. KJD was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000 and 500 mg/kg. Cyclophosphamide was used as a known genotoxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. In addition, the changes on the total white blood cells and differential counts on the lymphocytes, neutrophils, eosinophils, basophils and monocytes in the prepared blood smears were also conducted to observe the possible immunosuppress. The results obtained indicated that KJD shows no genotoxicity effects up to 2000 mg/kg dosing levels, but KJD shows slight increased trends in the blood total leukocyte numbers as pharmacological effects of immune stimulation. In addition, it is also considered that there were no problems from cytotoxicity of KJD tested in this study because the polychromatic erythrocyte ratio was detected as > 0.42 in all tested groups.

• Parasites, Hosts and Diseases
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• v.35 no.2
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• pp.135-138
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• 1997

This study was performed to observe intestinal patholoay ill early Infection by Centrocestus crnlctus. The flukes were in the lowermost part of the intfrvillous space of the duodenum and jejunum from 1 day to 7 days postinfection (PI). Thr stroma of villi around the young fluke was edematous and infiltrated by inflanlrllatory knells Luch as Iymphocytes, plasma cells and eosinophils. The crypt became mildly llyperplastic and villi were moderately atrophied at 4 days Pl. The intestinal lesion produced was confillrd to the areas around the fluke. The pathologic findings were not significarltly different between 1,000 and 5,000 metacercariae infection groups. It is suggested that the lesion should be produced by mechanical destruction of the fluke on the enteroepithelial cells.

• Journal of Veterinary Clinics
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• v.29 no.3
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• pp.247-249
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• 2012

An 8-year-old castrated male Cocker Spaniel (weighing 12.0 kg) was referred to the Kangwon National University Veterinary Teaching Hospital, with primary complaints of persistent hematuria. Diagnostic studies revealed neutrophilia, hematuria, proteinuria, abnormal irregular shaped hyperechoic lesion in urinary bladder. The lesion was demarcated from the intact region of bladder and consisted of eosinophils, macrophages, lymphocyte and fibrocytes. Based on the histopathological exam, the case was diagnosed as eosinophilic polypoid cystitis and treated by surgical removal and short-term medical therapy (meloxicam and amitriptyline). The therapy was successful and recurrence has not been occurred.

• Korean Journal of Veterinary Pathology
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• v.1 no.1
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• pp.46-52
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• 1997

The hematology and clinical chemistry data based on the well defined normal data are essential in the safety assessment. It is important that normal values of cliniclal pathology parameters would be optimized to be accurate. So the purpose of this experiment was to compare the ranges of normal data in hematology value and serum chemistry between both sexes of F344 and Wistar rats at 5, 10, 20, 40 and 90 weeks. of age. The neutrophils eosinophils and monocytes increased with age in male Wistar rats. And serum total bilirubin and potassium were highest and alkaline phosphatase was lowest at 90 weeks in Wistar rats. As compared with Wistar rat the serum aspartate aminotransferase of F344 rats increased with age in both sexes but inorganic phosphorus decreased with age. The serum alkaline phosphatase decreased with age and potassium was highest at 90weeks. These normal data would be useful in improving the accuracy of clinical pathology value in the safety assessment.

• Tuberculosis and Respiratory Diseases
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• v.75 no.4
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• pp.161-164
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• 2013

Eosinophilic pleural effusion (EPE) is defined as a pleural effusion that contains at least 10% eosinophils. EPE occurs due to a variety of causes such as blood or air in the pleural space, infection, malignancy, or an autoimmune disease. Undifferentiated connective tissue disease (UCTD) associated with eosinophilic pleural effusion is a rare condition generally characterized by the presence of the signs and symptoms but not fulfilling the existing classification criteria. We report a case involving a 67-year-old man with UCTD and EPE, who has been successfully treated with a single intrapleural corticosteroid injection.

• The Journal of Korean Medicine
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• v.25 no.1
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• pp.40-48
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• 2004

Background and Objectives: Aroma therapy using Eucalyptus Globulus essential oil for curing allergic rhinitis is an inhalation treatment which has been applied as an aid to oriental clinics which use a fundamental treatment such as acupuncture and herbs. There have been no studies so far performed on the effect of this oils' use, only . We aimed to find out the therapeutic effect of its exclusive use only on an Animal Model with Allergic Rhinitis. Materials and Methods: Sprague-Dawley rats were induced the allergic rhinitis by ovalbumin solution. And then, rats in the treated group were treated by diffusing Eucalyptus essential oil in a flask for 7 days. Compared with the treated group, rats in the control group were treated by diffusing normal saline in a flask for 7 days. We observed changes in glandular cells and mast cells in nasal mucosa and submucosa; also changes in the number of eosinophils and neutrophils in blood and the succus of rhinenchysis. Results: The number of neutrophils in the succus of rhinenchysis was significantly decreased in treated group compared with control group(p<0.05). The number of neutrophils in blood and mast cells in nasal mucosa were decreased in the treated group but, these were not significant statistically(p>0.05). Histologic changes showed edema; congestion and expansion of grandular cells in nasal submucosa and hypertrophy of epithelium in nasal mucosa were decreased in treated group compared with control group. Conclusions: The results may suggest that the inhalation treatment using Eucalyptus essential oil decreases the inflammatory response on an Animal Model with Allergic Rhinitis.

• CELLMED
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• v.3 no.1
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• pp.8.1-8.6
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• 2013

Ikwi-tang (IW) is an oriental medicine that has been used for the treatment of general symptoms due to a stomach yin deficiency. The aim of this study is to investigate precisely the effect of IW on allergic rhinitis (AR). We well show the effects and the mechanism of IW in the ovalbumin-sensitized AR model. IW significantly decreased the number of nasal/ear rubs and the increment of IgE levels in the AR mice. The levels of interferon-${\gamma}$ were enhanced while the levels of interleukin (IL)-4 were reduced in the spleen tissue of the IW-administered AR mice. Expressions of IL-$1{\beta}$ and cyclooxygenase-2 were inhibited by IW-administration in the nasal mucosa tissues. Infiltration of eosinophils and mast cells was decreased in the IW-administered AR mice. Our results indicate that IW may attenuate the development of AR by the inhibition of caspase-1 activity.

• The Journal of Korean Physical Therapy
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• v.20 no.2
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• pp.19-24
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• 2008

Purpose: The purpose of this study was to examine how norepinephrine affects immunity in patients over age 65. Methods: We enrolled 25 male and female subjects age 65 or older. A low frequency electroacupuncture (EA) device was used to stimulate acupoint Hogu (L14). The 2 Hz frequency EA was applied to the acupoint for 20 minutes. Leukocyte subtypes-including neutrophils, lymphocytes, monocytes, eosinophils, and basophil-were then measured. The immunoglobulins IgG and IgM were also quantified. The data were finally analyzed using Wilcoxon singed-rank test and regression test as part of the SPSS WIN v. 10.1 program. Results: As norepinephrine levels decreased after EA stimulation, neutrophil, lymphocyte, and monocyte levels increased, and eosinophil and neutrophils levels decreased. Neutrophil and monocyte levels did not change to a statistically significant degree, but eosinophil levels showed a statistically significant decrease (p<0.05). Immunoglobulin IgG showed a statistically significant increase (p<0.05). Conclusion: This study showed that norepinephrine does affect immunity in persons over the age of 65. This indicates that there is an interaction between the nervous system and the immune system, and interaction that plays a crucial roles in the body's immune resistance and homeostasis.