Browse > Article
http://dx.doi.org/10.5487/TR.2008.24.3.213

Micronucleus Test of Kong-Jin-Dan, a Polyherbal Formula, in Bone Marrow Cells of Male ICR Mice  

Lee, Sang-Nam (College of Oriental Medicine, Daegu Haany University)
Park, Ji-Ha (College of Oriental Medicine, Daegu Haany University)
Ku, Sae-Kwang (College of Oriental Medicine, Daegu Haany University)
Publication Information
Toxicological Research / v.24, no.3, 2008 , pp. 213-218 More about this Journal
Abstract
In this research, the genotoxic effects of Kong-Jin-Dan(KJD), a polyherbal formula were evaluated using the mouse micronucleus test. KJD was administered once a day for 2 continuous days by oral gavage to male ICR mice at doses of 2000, 1000 and 500 mg/kg. Cyclophosphamide was used as a known genotoxic agent in a positive control. The appearance of a micronucleus is used as an index for genotoxic potential. In addition, the changes on the total white blood cells and differential counts on the lymphocytes, neutrophils, eosinophils, basophils and monocytes in the prepared blood smears were also conducted to observe the possible immunosuppress. The results obtained indicated that KJD shows no genotoxicity effects up to 2000 mg/kg dosing levels, but KJD shows slight increased trends in the blood total leukocyte numbers as pharmacological effects of immune stimulation. In addition, it is also considered that there were no problems from cytotoxicity of KJD tested in this study because the polychromatic erythrocyte ratio was detected as > 0.42 in all tested groups.
Keywords
Kong-Jin-Dan$\beta$-Glucan; Micronucleus test; Genotoxicity; Mice; White blood cells;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Dourish, C.T. (1987). Effects of drugs on spontaneous motor activity in experimental psychopharmacology (Greenshaw A.J. and Dourish, C.T., Eds.), Humana Press, Clifton, pp. 325-334
2 Heddle, J.A. (1973). A rapid in vivo test for chromosome damage, Mutat. Res., 18, 187-190   DOI   ScienceOn
3 Kalantari, H., Larki, A. and Latifi, S.M. (2007). The genotoxicity study of garlic and pasipy herbal drops by peripheral blood micronucleus test. Acta Physiol. Hung., 94, 261-266   DOI
4 Matter, B. and Schmid, W. (1971). Trenimon-induced chromosomal damage in bone-marrow cells of six mammalian species, evaluated by the micronucleus test, Mutat. Res., 12, 417-425   DOI   ScienceOn
5 Schimid, W. (1975). The Micronucleus Test, Mutat. Res., 31, 9-15   DOI
6 Organization for Economic Co-Operation and Development (Ed.). (1997). Guideline for the Testing of Chemicals TG No. 474. Mammalian Erythrocyte Micronucleus Test, July 21, 1997
7 Kim, Y.S., Lee, J.D., Kim, C.H., Choi, D.Y., Park, D.S., Nam, S.S. and Kang, S.K. (1999). The Effect of Kongjindan Aqua-acupuncture on the CD4+ T cell count at al. in rats were administered with MTX. J. Korean Acup. Moxi. Soc 16, 179-202
8 Park, M.Y., Choi, H.Y., Kim, J.D., Lee, H.S. and Ku, S.K. (2007). Single oral dose toxicity test of Kong-Jin-Dan, a polyherbal formula in ICR mice. J. Appl. Pharmacology, 15, 245-251   과학기술학회마을   DOI
9 Lee, J.E., Kim, H.J., Choi, E.K, Chai, H.Y., Yun, Y.W., Kim, D.J., Nam, S.Y., Lee, B.J., Ahn, B.W., Kang, H.G. and Kim, Y.B. (2003). Four-week repeated-dose toxicity study on Pinellia Extract. Korean J. Lab. Anim. Sci., 19, 127-141
10 Irwin, S. (1968). Comprehensive observational assessment: Ia. A systemic, quantitative procedure for assessing the behavioral and physiological state of the mouse. Psychopharmacology, 13, 222-257   DOI
11 Korea Food and Drug Administration. (2005). Testing Guidelines for Safety Evaluation of Drugs (Notification No. 2005-60, issued by the Korea Food and Drug Administration on October 21, 2005)
12 Kim, Y.H. and Bae, M.J. (2001). The effect of Kong Jin Dan on the lipid metabolism in rats fed high fat-diet. J. East- West Med., 14, 61-78
13 Ashby, J. (1985). Is there a continuing role for the intraperitoneal injection route of exposure in short-term rodent genotoxicity assays? Mutat. Res., 156, 239-243   DOI   ScienceOn
14 Heddle, J.A., Hite, M., Kirkhart, B., Mavournin, K., MacGregor, J.T., Newell, G.W. and Salamone, M.F. (1983). The induction of micronuclei as a measure of genotoxicity: a report of the U.S. environmental protection agency genetox program, Mutat. Res., 123, 61-118   DOI   ScienceOn
15 Von Ledebur, M. and Schmid, W. (1973). The micronucleus test - methodological aspects, Mutat. Res., 19, 109-117   DOI   ScienceOn
16 Renner, H.W. (1990). In vivo effects of single or combined dietary antimutagens on mutagen-induced chromosomal aberrations, Mutat. Res., 244, 185-188   DOI   ScienceOn
17 Heddle, J.A., Stuart, E. and Salamone, M.F. (1984). The bone marrow micronucleus test in Handbook of mutagenicity test procedures (Kilbey, B.J., Legator, M., Nichols, W. and Ramel, C., Eds.), Elsevier, Amsterdam, pp. 441-457
18 Miyauchi, A., Hiramine, C., Tanaka, S. and Hojo, K. (1990). Differential effects of a single dose of cyclophosphamide on T cell subsets of the thymus and spleen in mice: flow cytofluorometry analysis, Tohoku J. Exp. Med., 162, 147-167   DOI
19 Grochow, L.B. (1996). Covalent-DNA binding drugs in The chemotherapy source book (Perry, M.C., Ed.), Williams & Wilkins, Baltimore, pp. 293-316
20 El-Bayoumy, K. (2001). The protective role of selenium on genetic damage and on cancer, Mutat. Res., 475, 123-139   DOI   ScienceOn