• Journal of Environmental Health Sciences
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• v.36 no.4
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• pp.279-287
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• 2010

In this study we examined the effects of water extract of garlic on carbon tetrachloride-induced liver injury, and demonstrated increased beneficial enzyme and anti-oxidant activity as well as histopathological changes (by Hematoxylin-Eosin (H&E) staining, Trichrome staining, and TEM examination), and showed that the treatment was dose-dependent and safe. A total of 42 male Sprague-Dawley rats were divided equally (n=7) into six groups. To induce hepatotoxicity in these subjects, carbon tetrachloride diluted in an equal volume of olive oil was intraperitoneally administrated at 0.5 ml/kg (0.20 g/kg/day) once a day for five days. Water extract of Korean-grown garlic was administered via a stomach sonde once a day, 5 days a week, for a total of 4 weeks. Groups received 0.35 g/kg (E1), 0.70 g/kg (E2), or 1.40 g/kg (E3), with the dose adjusted for body weight. Administration of garlic extract resulted in positive physiological effects in terms of reduced oxidative stress and toxicity, and induced functional changes in the liver. Comparing the subject groups (E1, E2, E3) administered different doses of garlic extract, the importance of morphological analysis in further studies is emphasized, because morphological changes indicating hepatotoxicity could occur, even though beneficial enzyme activities were found to be elevated.

• The Journal of Internal Korean Medicine
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• v.24 no.1
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• pp.21-32
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• 2003

Objective : The aim of this study is to investigate the inhibitory effect of Injinchunggantang on hepatic sclerosis induced by $CCl_4$. Methods : Weight, liver function test and complete blood cell count, gross findings, and findings on liver tissue of the past(Hematoxylin & Eosin stain, Masson-Trichrome stain) were studied. Results : When it comes to the change of rats' body weight, The $CCl_{4^+}$Injinchunggantang group lost far less weight than The $CCl_{4^-}$only group. In the liver function test, which is focused on various areas such as total cholesterol, alkaline phosphotase, albumin, aspartate transaminase, alanine transaminase, The $CCl_{4^+}$Injinchunggantang group was much more closer to normal limit than the $CCl_4$ only group. In the complete blood cell count, including white blood cell, red blood cell, hemoglobin, hematocrite, platelet, The $CCl_{4^+}$Injinchunggantang group significantly closer to normal limit than $CCl_{4^-}$only group. In the gross findings of hepatic fibrosis models, Injinchunggantang showed inhibitory effect on hepatic fibrosis in the order. In the past findings of hepatic fibrosis models in Hematoxylin & Eosin, Masson-Trichrome staining, the liver in $CCl_{4^-}$only group showed atrophy and necrotic change with white nodules, whereas that of $CCl_{4^+}$Injinchunggantang group showed lesser significant change with the well_preserved tone of the tissue. In the extent of the inhibition of the hepatic fibrosis, the Injinchunggantang group showed statistically significant inhibitory effect(p<0.05) in the sclerosis model. Conclusions : These results show that Injinchunggantang have inhibitory effect on hepatic sclerosis induced by $CCl_4$ and further ultimately prevent liver cirrhosis. To obtain more credible results in this experiment, the invention of a new experimental model more similar to human hepatic sclerosis is still needed.

• Reproductive and Developmental Biology
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• v.34 no.4
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• pp.299-304
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• 2010

To identify the treatment effect of lactic acid bacteria for diabetes, the treatment effects of a single administration of acarbose (a diabetes treatment drug) or lactic acid bacteria, and the mixture of acarbose and lactic acid bacteria on diabetes in a type 1 diabetes animal model, were studied. In this study, streptozotocin was inoculated into a Sprague-Dawley rat to induce diabetes, and sham control (Sham), diabetic control (STZ), STZ and composition with live cell, STZ and composition with heat killed cell, STZ and composition with drugs (acarbose) were orally administered. Then the treatment effect on diabetes was observed by measuring the body weight, blood glucose, and serum lipid. For the histopathological examination of the pancreas, the Langerhans islet of the pancreas was observed using hematoxylin and eosin staining, and the renal cortex, outer medullar, and inner medullar were also observed. The induced diabetes decreased the body weight, and the fasting blood glucose level decreased in the lactic-acid-bacteria-administered group and the mixture-administered group. In addition, the probiotic resulted in the greatest decrease in the serum cholesterol level, which is closely related to diabetes. Also, the hematoxylin and eosin staining of the Langerhans islet showed that the reduction in the size of the Langerhans islet slowed in the lactic-acid-bacteria-administered group. The histopathological examination confirmed that the symptoms of diabetic nephropathy decreased in the group to which viable bacteria and acarbose were administered, unlike in the group to which dead bacteria was administered. The mixture of lactic acid bacteria and acarbose and the single administration of lactic acid bacteria or acarbose had treatment effects on the size of the Langerhans islet and of the kidney histopathology. Thus, it is believed that lactic acid bacteria have treatment effects on diabetes and can be used as supplements for the treatment of diabetes.

• The Journal of Korean Academy of Prosthodontics
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• v.15 no.1
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• pp.26-33
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• 1977

Present study investigated the effect of Nuva-Lite irradiation on the labial oral mucosa of the albino rats. The specimens were embedded in paraffin wax and stained with histological and histochemical procedures such as hematoxylin-eosin stain, Feulgen reaction, methyl green-pyronin stain, alloxan Schiff reaction, -SH group reaction, PAS reaction, colloidal iron reaction and toluidine blue stain. The specimens 24 hours to 3 days after ultraviolet irradiation exhibited mild intracellular edema in the prickle cell layer. On 6th and 10th day after irradiation rete peg hyperplasia was prominent. Ultraviolet irradiation caused diminution of Feulgen reacted DNA. However, sulfhydril and ${\alpha}$-amino acid radicals were increased at the upper layer of stratified squamous epithelium after irradiation.

• Journal of the Korea Convergence Society
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• v.8 no.4
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• pp.123-129
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• 2017

This study has been conducted to see influence of fibroid hepatic tissue on alpha-tocopherol and its histologic changes. Experimental groups were divided into 3 groups, one where fibrosis was caused by carbon tetrachloride and the other where alpha-tocopherol and carbon tetrachloride were administered together and another normal group. Results of test showed fibrosis reaction is weak compared to the group administered with carbon tetrachloride in the group administered with alpha-tocopherol. In conclusion, we could observe proliferation of collagen fibers through Hematoxylin&Eosin and Massom trichrom dye and increase in ALT, AST, ALP in fibroid hepatic tissues, indicating convergence anti-oxidation reaction by changes of alpha-tocopherol intake. Thus, it is considered that we can expect to utilize alpha-tocopherol as secondary treatment and find clinical meanings of diagnosis on hepatic diseases through results of this study.

• Restorative Dentistry and Endodontics
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• v.34 no.4
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• pp.356-363
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• 2009

The purpose of this study was to evaluate the viability of periodontal ligament cells in rat teeth using slow cryo-preservation method under pressure by means of MTT assay and WST-1 assay. Eighteen teeth of Sprague-Dawley white female rats of 4 week-old were used for each group. Both sides of the first and second maxillary molars were extracted as atraumatically as possible under Tiletamine anesthesia. The experimental groups were group 1 (Immediate control), group 2 (Cold preservation at $4^{\circ}C$for 1 week), group 3 (Slow freezing), group 4 (Slow freezing under pressure of 3 MPa). F-medium and 10% DMSO were used as preservation medium and cryo-protectant. For cryo-preservation groups, thawing was performed in $37^{\circ}C$water bath, then MTT assay and WST-1 assay were processed. One way ANOVA and Tukey method were performed at the 95% level of confidence. The values of optical density obtained by MTT assay and WST-1 were divided by the values of eosin staining for tissue volume standardization. In both MTT and WST-1 assay, group 4 showed significantly higher viability of periodontal ligament cells than group 2 and 3 (p < 0.05), but showed lower viability than immediate control group. By the results of this study, slow cryo-preservation method under pressure suggests the possibility for long term cryo-preservation of the teeth.

• Journal of Environmental Science International
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• v.12 no.3
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• pp.319-324
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• 2003

The characteristics of photocatalytic decomposition of dye waste water by titanium dioxide was studied in a batch reactor under constant strength of ultra-violet ray. The decomposition rate of methyl orange by TiO$_2$ was pseudo-first order, anatase type TiO$_2$ was more effective than rutile type below the dosage of 5g. The decomposition rate was increased with decreasing initial pH, increasing reaction temperature and oxidant concentration. The decomposition rate of water soluble dyes was decreased in order of rhodamine B>eosin Y>methyl orange.

• Rapid Communication in Photoscience
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• v.2 no.3
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• pp.72-75
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• 2013

Bulk nanobubble is one of the nanoscopic gaseous state found in the solution. We measured transient absorption spectra of eosin Y in the excited triplet state ($^3EY^{2-*}$) to elucidate differences in the dissipation process of the bulk nanobubble of oxygen molecule at air pressure. The time dependence of the oxygen dissipation process was classified three time domains (P1, P2 and P3). The comparison of ordinary bubbling method gave different results at P3 in contrast to similar results at P1 and P2, indicating the existence of characteristic dissolving state in the case of nanobubble.

• The Korean Journal of Zoology
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• v.9 no.1
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• pp.39-45
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• 1966

백서(Rattus horvegicus var. albinus) 50 두를 키우며 자성백서의 발정을 질정도말법으로 확인하여 임신케 한후 각 단계별로 80개의 배를 얻었다. 고정액은 Bouin's solution 과 Carnoy's solution II였으며 표본은 5-7$\mu$의 두께로 연속절편을 만들었다. 염색은 Delafield's hematoxylin 과 eosin-Y의 이중염색법과 신경섬유를 관찰하기 위하여 Ramoney Cajal's silver-nitrate method를 사용하였다. 실험결과는 안와가 stage 17에서 나타났고 안포가 stage 18에서 형성되었다. 심안포가 stage 19에서 형성되었고 망막판과 수정체판이 stage 21에서 형성되었으며 체표외배엽과 접촉하고 있는 안포의 부분은 stage 22(A)에서 안배를 형성하기 위해 몰입되었다. state 22 (B)에서 수정체공은 아직 열려 있었고 stage 23(A)에서 색소립이 망막의 색소층에 나타났고 수정체공이 닫혀졌다. 수정체와는 stage 23(B)에서 체표외배엽으로부터 분리되었고 수정체 내벽세포의 신장이 stage23(C)에서 시작되었다. 수정체강은 stage 25에서 수정체섬유에 의해서 인멸되었고 stage 26에서 홍채가 형성되기 시작하였다.

• The Journal of the Korean dental association
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• v.18 no.8 s.137
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• pp.657-667
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• 1980

This study was undertaken to observe the salivary gland of the white rat when exposed to single and fractionated doses of Cobalt-60 irradiation. One hundred fifty white rats of the experiment were devided into control and 3 experimental groups. In experimental groups, group I receivcd 1200 rads everyweek untill 4800 rads reached, group II received 1500 rads and group III received 2000 rads with single dose. irradiation was carried out using a RAC-120 Cobalt-60 Teletherapy Unit with a dose rate 84.3 r/min, field size 4×5 cm measured at 80 from source. Rats were serially sacrificed at the following postirradiation time intervals: 1, 3, 5, 7, 10, 14, 21, 28, 35, and 42days. At sacrifice, the parotid and submandibular glands were dissected out in toto, and stained with: 1) hematoxylin and eosin; 2) periodic acid Shiff; 3) toludine blue.