• Title/Summary/Keyword: Enzyme-Linked Immunosorbent Assay

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Production and Characterization of Monoclonal Antibodies to a Generic Hapten for-Class-Specific Determination of Organophosphorus Pesticides

  • Jang, Mi-Soon;Lee, Soo-Jung;Xue, Xiaoping;Kwon, Hyuk-Man;Ra, Choon-Sup;Lee, Yong-Tae;Chung, Tae-Wan
    • Bulletin of the Korean Chemical Society
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    • v.23 no.8
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    • pp.1116-1119
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    • 2002
  • Monoclonal antibodies have been generated against a generic hapten, ο,ο-diethyl ο-(5-carboxy-2-fluorophenyl) phosphorothioate, for the determination of organophosphorus (OP) pesticides in a class-specific manner. In an indirect competitive enzyme-linked immunosorbent assay (ELISA) format, employing a heterologous coating antigen, these monoclonal antibodies showed desirable properties for use in the class-specific determination, i.e., broad specificity and high sensitivity. The IC50 values of four commonly used ο,ο-diethyl OP pesticides were fairly uniform ranging from 0.1 to 0.3 ㎛/mL. The IC50 values of three ο,ο-dimethyl derivatives were between 0.3 and 1.4 ㎛/mL. These values, together with the limits of detection (LOD), were better, in terms of the specificity and sensitivity, compared with the values obtained previously with polyclonal antibodies.

Seroprevalence of Antigens to Bovine Viral Diarrhea Virus in Korean Calves of the Shown Healthy, Digestive and Respiratory Symptom (한우송아지에서 ELISA를 이용한 소 바이러스성 설사병 바이러스 항원 검출)

  • Chon, Seung-Ki;Park, Jin-Ho;Kim, Nam-Soo
    • Journal of Veterinary Clinics
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    • v.24 no.2
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    • pp.150-153
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    • 2007
  • The aim of this study was to investigate the prevalence of bovine viral diarrhea virus (BVDV) infection in Chonbuk province. Blood samples were taken from 92 korean calves to determined their serological status against BVDV, Capture enzyme-linked immunosorbent assay (ELISA) was used to test for antigen. The number of seropositive calves ranged from 3.3% to 12.9%. Antigens against BVDV were detected in 3.3% of healthy calves, 6.4% of digestive symptom calves, 12.9% of respiratory symptom calves, respectively. Sex and age of calves had no significant differences on the prevalence of BVDV. The results indicate that transmission of BVDV may have become exposed as a result of contact with acute infected or persistently infected cattle.

Detection Rate of Bovine Viral Diarrhea Virus in Dairy Calves with Capture-ELISA (젖소송아지에서 ELISA를 이용한 소 바이러스성 설사병 바이러스 검출률)

  • Chon, Seung-Ki;Kim, Nam-Soo
    • Journal of Veterinary Clinics
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    • v.24 no.2
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    • pp.169-171
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    • 2007
  • The aim of this study was to detect bovine viral diarrhea virus (BVDV) from calves in Chonbuk province. Blood samples were taken from ninety-two dairy calves. Capture enzyme-linked immunosorbent assay (ELISA) was used to detect BVDV. BVDV were detected in eight out of ninety-two (8.6%) dairy calves. BVDV were detected in one of twenty five of female calves and one of twenty three of male calves of 4 months old, whereas in the 5 months age group, BVDV were detected in low of twenty three of female calves and two of twenty one of male calves. There were no significant differences (p>0.05) in the detection rate of BVDV on the basis of sex. On the other hand, ages of calves had significant differences (p<0.05) on the prevalence of BVDV.

Studies on Enzyme-linked Immunosorbent Assay(ELISA) for Detection of Antibody to Mycobacterium bovis in Serum and Milk (Enzyme-linked Immunosorbent Assay(ELISA)를 이용한 혈청 및 원유 중의 Mycobacterium bovis 항체 검출에 관한 연구)

  • 심항섭;국정희;박병옥;김성열;박유순
    • Korean Journal of Veterinary Service
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    • v.20 no.2
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    • pp.133-142
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    • 1997
  • In order to supplement a diagnostic method for detection of infectious cattle to bovine tuberculosis, performed ELISA for detection of antibody to if bovis in serum and milk. The diagnostic efficacy of the established ELISA was compared with test of the tuberculin skin test for bovine tuberculosis. The positive corresponding rate of serum ELISA and tuberculin skin test showed 84.3%, milk ELISA and tuberculin skin test showed 75.0%, milk ELISA and serum ELISA showed 75.0% respectively. Comparison of the serum and milk to tuberculin antibody concentration in tuberculin positive cattle, the milk contained 1/100-1/150 concentration compared serum tuberculin concentration. The established ELISA was considered efficient for detection of antibodies to M bovis in serum and milk.

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Enzyme-linked immunosorbent assay for detection of Trichinella spiralis antibodies and the surveillance of selected pig breeding farms in the Republic of Korea

  • Wee, Sung-Hwan;Lee, Chung-Gil;Joo, Hoo-Don;Kang, Yung-Bai
    • Parasites, Hosts and Diseases
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    • v.39 no.3
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    • pp.261-264
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    • 2001
  • Trichinellosis is a parasitic zoonosis of public health importance. It is caused by Trichinella spiralis which has a wide host range including humans. In the present communication, the ELISA technique was employed on a total of 803 blood samples from 7 selected pig breeding farms in 1996 for diagnosis and surveillance of trichinellosis. Out of the entire 803 samples, nine were found to be suspected while one was positive by ELISA. But western blot analyses employed for further confirmation have shown that all of 10 samples did not react to larval excretory-secretory product antigens. These results indicate that pig breeding farms included in the present study are free from trichinellosis . However, it does not mean Korea is free from trichinellosis since human trichinellosis has recently been reported. The necessity of continued surveillance for trichinellosis in both pigs and wild animals was discussed.

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A study on analytical methods for polycyclic aromatic hydrocarbons in foods (식품 중 다환방향족탄화수소 분석법 연구)

  • Kim, Yong-Yeon;Shin, Han-Seung
    • Food Science and Industry
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    • v.55 no.1
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    • pp.45-57
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    • 2022
  • This study was proceeded the analytical methods using various analytical instruments for polycyclic aromatic hydrocarbons (PAHs) in food products. Various analytical methods were developed to determine levels of PAHs including benzo[a]pyrene, benzo[a]anthracene, benzo[b]fluoranthene, and chrysene formed in various food products using gas chromatography-mass spectrometry (GC-MS), enzyme-linked immunosorbent assay (ELISA) and raman spectroscopy. Recently, the rapid on-site response for the detection of hazardous substances in food aims to develop an onsite rapid detection of a simplified technical analysis method to reduce the time and cost required for analysis of PAHs. Current PAHs detection methods have been reviewed along with new raman spectroscopy analytical method.

Assay of Cellobiohydrolnse by Column Single Immunodiffusion and Enzyme tinted Immunosorbent Assay (면역화학적 방법에 의한 Cellobiohydrolase 정량)

  • 오태광;고영희;김정일;박관희
    • Microbiology and Biotechnology Letters
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    • v.16 no.3
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    • pp.226-230
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    • 1988
  • Antibody against cellobiohydrolase purified from Trichoderma viride had been obtained by injection to rabbit. The antibody had a high specificity against the cellobiohydroase evidienced by absence of immunological reaction to other isozymes from Trichoderma viride. Assay limit of cellobiohydrolase was 1-10 $\mu\textrm{g}$ by column single immunodiffusion and by enzyme linked immunosorbent assay, it was 10-140 ng and 100-1200 pg when the dilution of antibody was 10$^{-6}$ and 10$^{-5}$, respectively.

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Serodiagnosis of Typhoid Fever by Enzyme-Linked Immunosorbent Assay(ELISA) (효소면역측정법에 의한 장티푸스의 혈청학적 진단)

  • Hwang, Eung-Soo;Cho, Myung-Je;Cha, Chang-Yong;Choe, Kang-Won;Lee, Seung-Hoon;Chang, Woo-Hyun
    • The Journal of the Korean Society for Microbiology
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    • v.21 no.3
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    • pp.387-391
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    • 1986
  • Serum samples from 51 patients with clinically suspected typhoid fever were tested for immunoglobulin G (IgG), IgM and IgA antibodies against the whole bacteria antigen of Salmonella typhi by an enzyme-linked immunosorbent assay. The levels of IgG and IgA antibody to-whole bacteria antigen were higher in the culture-proven patients than in controls. The levels of IgM antibody to- whole bacteria antigen showed better discrimination between culture negative patients and controls than those of IgG or IgA antibody to-whole bacteria antigen. The enzyme-linked immunosorbent assay was much more sensitive than the Widal test. It would be a useful tool for the diagnosis of typhoid fever with a single serum sample.

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Comparative evaluation of indirect immunofluorescent antibody test with enzyme-linked immunosorbent assay in serodiagnosis of human neurocysticercosls (뇌낭미충증의 혈청학적 진단에 있어서 간접 형광항체 반응 및 효소연결성 면역흡착 검사의 비교 평가)

  • Eom, Gi-Seon;Jo, Seung-Yeol;Im, Han-Jong
    • Parasites, Hosts and Diseases
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    • v.26 no.1
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    • pp.27-32
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    • 1988
  • The applicability of indirect immunoftuorescent antibody test (IFAT) was compared with enzyme-linked immunosorbent assay (ELISA) in sera from 163 cases of confirmed neurocysticercosis, 101 other neurologic and parasitic diseases and 100 normal controls. As antigen, frozen sections of a Taenia solium metacestode from a human brain was used in IFAT and cystic fluid was used in ELISA. For the detection of specific IgG antibody, IFAT was equally sensitive (89.6%) and specific (85.1%) as ELISA. The antibody titers by IFAT were correspondingly increased with mean absorbance of ELISA. The corresponding rate of positivity in the two techniques was 90.8%. Except for the difficulty in detecting antibodies in cerebrospinal fluid (CSF), IFAT was concluded to be very useful for the serodiagnosis of human neurocysticercosis.

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