Bulletin of the Korean Chemical Society

  • 제23권8호
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  • Pages.1116-1119
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  • 2002
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  • 0253-2964(pISSN)
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  • 1229-5949(eISSN)
대한화학회 (Korean Chemical Society)
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Production and Characterization of Monoclonal Antibodies to a Generic Hapten for-Class-Specific Determination of Organophosphorus Pesticides

  • 발행 : 2002.08.20
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초록

Monoclonal antibodies have been generated against a generic hapten, ο,ο-diethyl ο-(5-carboxy-2-fluorophenyl) phosphorothioate, for the determination of organophosphorus (OP) pesticides in a class-specific manner. In an indirect competitive enzyme-linked immunosorbent assay (ELISA) format, employing a heterologous coating antigen, these monoclonal antibodies showed desirable properties for use in the class-specific determination, i.e., broad specificity and high sensitivity. The IC50 values of four commonly used ο,ο-diethyl OP pesticides were fairly uniform ranging from 0.1 to 0.3 ㎛/mL. The IC50 values of three ο,ο-dimethyl derivatives were between 0.3 and 1.4 ㎛/mL. These values, together with the limits of detection (LOD), were better, in terms of the specificity and sensitivity, compared with the values obtained previously with polyclonal antibodies.

키워드

참고문헌

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피인용 문헌

  1. Development of a Class-specific Immunochromatographic Strip Test for the Rapid Detection of Organophosphorus Pesticides With a Thiophosphate Group vol.29, pp.4, 2010, https://doi.org/10.1089/hyb.2009.0111
  2. Destruction and Detection of Chemical Warfare Agents vol.111, pp.9, 2011, https://doi.org/10.1021/cr100193y
  3. A Novel Photoelectrochemical Sensor for the Organophosphorus Pesticide Dichlofenthion Based on Nanometer-Sized Titania Coupled with a Screen-Printed Electrode vol.83, pp.13, 2011, https://doi.org/10.1021/ac200706k
  4. Development of an Enzyme Linked Immunosorbent Assay and an Immunochromatographic Assay for Detection of Organophosphorus Pesticides in Different Agricultural Products vol.7, pp.12, 2012, https://doi.org/10.1371/journal.pone.0053099
  5. Multi-analyte enzyme-linked immunosorbent assay for organophosphorus pesticides and neonicotinoid insecticides using a bispecific monoclonal antibody vol.5, pp.6, 2013, https://doi.org/10.1039/c3ay26398c
  6. Immunoassay Development for the Class-Specific Assay for Types I and II Pyrethroid Insecticides in Water Samples vol.15, pp.1, 2010, https://doi.org/10.3390/molecules15010164
  7. Synthesis of three haptens for the class-specific immunoassay of O,O-dimethyl organophosphorus pesticides and effect of hapten heterology on immunoassay sensitivity vol.615, pp.2, 2002, https://doi.org/10.1016/j.aca.2008.03.050
  8. Effect of hapten structures on specific and sensitive enzyme-linked immunosorbent assays for N-methylcarbamate insecticide metolcarb vol.625, pp.1, 2002, https://doi.org/10.1016/j.aca.2008.07.003
  9. Production and characterization of a broad-specificity polyclonal antibody for O,O-diethyl organophosphorus pesticides and a quantitative structure–activity relationship study of antibody recogn vol.647, pp.1, 2009, https://doi.org/10.1016/j.aca.2009.05.025
  10. Application of computer-assisted molecular modeling for immunoassay of low molecular weight food contaminants: A review vol.647, pp.2, 2009, https://doi.org/10.1016/j.aca.2009.06.003
  11. Production of the class-specific antibody and development of direct competitive ELISA for multi-residue detection of organophosphorus pesticides vol.23, pp.2, 2012, https://doi.org/10.1080/09540105.2011.608120
  12. Immunochemical and molecular characteristics of monoclonal antibodies against organophosphorus pesticides and effect of hapten structures on immunoassay selectivity vol.26, pp.1, 2002, https://doi.org/10.1080/09540105.2013.873390