• Title/Summary/Keyword: Environmental stresses

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The Stress-Activated Signaling (SAS) Pathways of a Human Fungal Pathogen, Cryptococcus neoformans

  • Jung, Kwang-Woo;Bahn, Yong-Sun
    • Mycobiology
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    • v.37 no.3
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    • pp.161-170
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    • 2009
  • Cryptococcus neoformans is a basidiomycete human fungal pathogen that causes meningoencephalitis in both immunocompromised and immunocompetent individuals. The ability to sense and respond to diverse extracellular signals is essential for the pathogen to infect and cause disease in the host. Four major stress-activated signaling (SAS) pathways have been characterized in C. neoformans, including the HOG (high osmolarity glycerol response), PKC/Mpk1 MAPK (mitogen-activated protein kinase), calcium-dependent calcineurin, and RAS signaling pathways. The HOG pathway in C. neoformans not only controls responses to diverse environmental stresses, including osmotic shock, UV irradiation, oxidative stress, heavy metal stress, antifungal drugs, toxic metabolites, and high temperature, but also regulates ergosterol biosynthesis. The PKC(protein kinase C)/Mpk1 pathway in C. neoformans is involved in a variety of stress responses, including osmotic, oxidative, and nitrosative stresses and breaches of cell wall integrity. The $Ca^{2+}$/calmodulin- and Ras-signaling pathways also play critical roles in adaptation to certain environmental stresses, such as high temperature and sexual differentiation. Perturbation of the SAS pathways not only impairs the ability of C. neoformans to resist a variety of environmental stresses during host infection, but also affects production of virulence factors, such as capsule and melanin. A drug(s) capable of targeting signaling components of the SAS pathway will be effective for treatment of cryptococcosis.

Functional Characterization of PR-1 Protein, β-1,3-Glucanase and Chitinase Genes During Defense Response to Biotic and Abiotic Stresses in Capsicum annuum

  • Hong, Jeum-Kyu;Hwang, Byung-Kook
    • The Plant Pathology Journal
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    • v.21 no.3
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    • pp.195-206
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    • 2005
  • Spatial and temporal expression of pathogenesis-related (PR) gene and proteins has been recognized as inducible defense response in pepper plants. Gene expression and/or protein accumulation of PR-1, $\beta-1,3-glucanase$ and chitinase was predominantly found in pepper plants during the inoculations by Xanthomonas campestris pv. vesicatoria, Phytophthora capsici and Colletotrichum coccodes. PR-1 and chitinase genes were also induced in pepper plants in response to environmental stresses, such as high salinity and drought. PR-1 and chitinase gene expressions by biotic and abiotic stresses were regulated by their own promoter regions containing several stress-related cis-acting elements. Overexpression of pepper PR-1 or chitinase genes in heterogeneous transgenic plants showed enhanced disease resistance as well as environmental stress tolerances. In this review, we focused on the putative function of pepper PR-1, $\beta-1,3-glucanase$ and chitinase proteins and/or genes at the biochemical, molecular and cytological aspects.

Cross-talk between Phosphate Starvation and Other Environmental Stress Signaling Pathways in Plants

  • Baek, Dongwon;Chun, Hyun Jin;Yun, Dae-Jin;Kim, Min Chul
    • Molecules and Cells
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    • v.40 no.10
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    • pp.697-705
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    • 2017
  • The maintenance of inorganic phosphate (Pi) homeostasis is essential for plant growth and yield. Plants have evolved strategies to cope with Pi starvation at the transcriptional, post-transcriptional, and post-translational levels, which maximizes its availability. Many transcription factors, miRNAs, and transporters participate in the Pi starvation signaling pathway where their activities are modulated by sugar and phytohormone signaling. Environmental stresses significantly affect the uptake and utilization of nutrients by plants, but their effects on the Pi starvation response remain unclear. Recently, we reported that Pi starvation signaling is affected by abiotic stresses such as salt, abscisic acid, and drought. In this review, we identified transcription factors, such as MYB, WRKY, and zinc finger transcription factors with functions in Pi starvation and other environmental stress signaling. In silico analysis of the promoter regions of Pi starvation-responsive genes, including phosphate transporters, microRNAs, and phosphate starvation-induced genes, suggest that their expression may be regulated by other environmental stresses, such as hormones, drought, cold, heat, and pathogens as well as by Pi starvation. Thus, we suggest the possibility of cross-talk between Pi starvation signaling and other environmental stress signaling pathways.

Analysis of ZjWRKY3, ZjWRKY7 induced by multiple stress in Zoysia japonica (다양한 스트레스에 유도되는 들잔디 ZjWRKY3, ZjWRKY7의 분석)

  • Kim, Woo-Nam;Song, In-Ja;Kang, Hong-Gyu;Sun, Hyeon-Jin;Yang, Dae-Hwa;Lee, Yong-Eok;Kwon, Yong-Ik;Lee, Hyo-Yeon
    • Journal of Plant Biotechnology
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    • v.44 no.3
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    • pp.220-228
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    • 2017
  • Many crops including cereals, tuber crops, feeds, and turf grasses are often damaged by various environmental stresses such as drought, salt, cold, and high temperature, causing the reduction of their productivity. Plants are sessile and cannot escape from environmental stresses. Thus, plants evolve in the direction of overcoming the environmental stresses. Some plant genes such as ARF, ABI3, NAC, HSF, and WRKY are known to respond to environmental stresses as they transcriptionally regulate the stress response pathways. For example, the OsWRKY76 gene contributes to the enhanced resistance to low temperatures and pathogenic infections. The AtWRKY28 also plays a role in environmental stresses. Zoysiagrass (Zoysia japonica Steud.) is popularly grown for gardens and golf courses. However, the function of the WRKY gene, another environmental stress-related gene, is not known in zoysiagrass. In this study, the ZjWRKY3 and ZjWRKY7 genes with one shared WRKY domain have been isolated in zoysiagrass. The expression of these genes increased in response to low temperature, drought, and salt stresses. Furthermore, the infection of the brown patch-causing Rhozoctonia solani induced the expression of ZjWRKY3 and ZjWRKY7. The corresponding proteins bind to the W-box of the Zjchi promoter, possibly regulating their transcriptions. The researchers suggest that the ZjWRKY3 and ZjWRKY7 genes transcriptionally regulate abiotic and biotic stress related downstream genes.

Multifactor Balance Concept as a Primary Countermeasure for Environmental Stresses of Crops (작물의 일차적 재해방지 요건으로서의 다요인 평형조절 개념)

  • 박천서
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.34 no.s02
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    • pp.1-12
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    • 1989
  • The primary countermeasure for environmental stresses of crops grown in Korea would be to maintain top soil content of available B for upland crops and Si for low land rice in balance with other nutrient elements such as N, P and K, so as to maintain those nutrient balances in plants. Development of standard levels of elements in soils for balances uptake of those elements by plants are needed under the multi nutrient factor balance concept using the soil test results.

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COMPARISON OF ENVIRONMENTAL STRESS DIAGNOSIS OF CUCUMBER PLANT USING VARIOUS PHYSIOLOGICAL INSTRUMENTS

  • Suh, S.R.;Chung, G.C.;Sung, J.H.;Ryu, Y.S.;Lee, K.H.
    • Proceedings of the Korean Society for Agricultural Machinery Conference
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    • 2000.11c
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    • pp.736-743
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    • 2000
  • This paper represents our efforts to diagnose environmental stresses using physiological instruments in cucumber plants. The stresses could be detected by measuring and analyzing the difference of chlorophyll content, photosynthetic efficiency(Fv/Fm), differential temperature(DT), stomatal resistance and light absorbance values between treated and controlled plants. From the all over experiments, the stresses could be first diagnosed on the 1st to 5th day after treatment and the overall diagnosis rate was estimated at more than 50%.

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Residual stress of cold-formed thick-walled steel rectangular hollow sections

  • Zhang, Xingzhao;Liu, Su;Zhao, Mingshan;Chiew, Sing-Ping
    • Steel and Composite Structures
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    • v.22 no.4
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    • pp.837-853
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    • 2016
  • This paper presents the experimental and numerical study on the distribution of transverse and longitudinal residual stresses in cold-formed thick-walled structural steel rectangular hollow sections manufactured by indirect technique. Hole-drilling method is employed to measure the magnitude of the transverse and longitudinal surface residual stress distribution, and the effects of the residual stresses are evaluated qualitatively by sectioning method. It is shown that compared to normal cold-formed thin-walled structural hollow sections (SHS), the cold-formed thick-walled SHS has similar level of residual stress in the flat area but higher residual stresses in the corner and welding areas. Both the transverse and longitudinal residual stresses tend to open the section. In order to predict the surface residual stresses in the corners of the cold-formed thick-walled SHS, an analytical model is developed. 2D finite element simulation of the cold bending process is conducted to validate the analytical approach. It is shown that in analyzing bending for thick-walled sections, shifting of neutral axis must be considered, since it would lead to nonlinear and non-symmetrical distribution of stresses through the thickness. This phenomenon leads to the fact that cold-formed thick-walled SHSs has different distribution and magnitude of the residual stresses from the cold-formed thin-walled SHSs.

Curling Behavior of Long-Span Concrete Pavement Slab under Environmental Loads (환경하중에 의한 장스팬 콘크리트 포장 슬래브의 컬링 거동 특성 분석)

  • Kim, Seong-Min;Shim, Jae-Soo;Park, Hee-Beom;Yun, Dong-Ju
    • International Journal of Highway Engineering
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    • v.11 no.3
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    • pp.151-161
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    • 2009
  • This study was conducted to investigate the characteristics of the curling behavior of long-span pavement slabs under environmental loads. By developing and using finite element models of the long-span pavement slabs, the stress distribution and the effects of slab length, slab thickness, stiffness of underlying layers, and the restraints of the slab ends on the curling behavior were analyzed. In addition, the field experiments were performed with the actual long-span pavement slab to obtain the curling behavior of the real structure under environmental loads. As a result of this study, it was found that the vertical displacements of the long-span pavement slab along the centerline due to the curling behavior were zero except for the areas near the slab ends, and the curling stresses were maximum and constant where the displacements were zero. The slab length and the stiffness of underlying layers did not affect the maximum curling stresses. The restraints at the slab ends made the curling stresses occur near the slab ends, but did not much affect the maximum curling stresses.

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Behavior of girth-welded buried steel pipes under external pressure (원주 용접된 압력 매설강관의 거동 분석)

  • Jeon, Juntai;Lee, Chinhyung;Chang, Kyongho
    • Journal of the Society of Disaster Information
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    • v.11 no.1
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    • pp.1-8
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    • 2015
  • This paper presents finite element (FE) analyses to clarify the effects of external pressure on the residual stresses in a girth-welded steel pipe. At first, FE simulation of the girth welding process is carried out to obtain the weld-induced residual stresses employing sequentially coupled three-dimensional (3-D) thermo-mechanical FE formulation. Then, 3-D elastic-plastic FE analyses incorporating the residual stresses and plastic strains obtained from the preceding FE simulation are performed to investigate the residual stress behavior in the girth-welded pipe under external pressure. The FE analysis results show that the hoop compressive stresses induced by the external pressure significantly alter the hoop residual stresses in the course of the mechanical loading.

Investigation on Behaviors of Concrete Interfaces Repaired Using Anchors (앵커로 보수한 콘크리트 계면 거동의 고찰)

  • Song Hyung-Soo;Lee Chin-Yong;Yoon Dong-Yong;Min Chang-Shik;Choi Dong-Uk
    • Journal of the Korea Concrete Institute
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    • v.17 no.6 s.90
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    • pp.885-892
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    • 2005
  • Recently, the damaged concrete structures are often strengthened or repaired using the polymer concrete or the polymer cement mortar. In the repaired concrete structures at early ages, internal stresses could be developed due to the differential drying shrinkage of the repair material. Due to the difference of the thermal coefficients of the repair material and existing concrete, additional stresses also could be developed as the structures are subjected to the ambient temperature changes. Theses environmentally-induced stresses can sometimes be large enough to cause damage to the structures, such as debonding of the interface between the two materials. In this study, a rational procedure was developed where anchors can be designed and installed to prevent damages in such structures by thermally-induced stresses. Finally, through the experimental study and numerical study, the effects of the repair method using anchors with debonding was investigated and discussed the results.