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http://dx.doi.org/10.5010/JPB.2017.44.3.220

Analysis of ZjWRKY3, ZjWRKY7 induced by multiple stress in Zoysia japonica  

Kim, Woo-Nam (Faculty of Biotechnology, Jeju National University)
Song, In-Ja (Subtropical Horticulture Research Institute, Jeju National University)
Kang, Hong-Gyu (Subtropical Horticulture Research Institute, Jeju National University)
Sun, Hyeon-Jin (Subtropical Horticulture Research Institute, Jeju National University)
Yang, Dae-Hwa (Subtropical Horticulture Research Institute, Jeju National University)
Lee, Yong-Eok (Department of Medical Bioscience, Dongguk University)
Kwon, Yong-Ik (Subtropical Horticulture Research Institute, Jeju National University)
Lee, Hyo-Yeon (Faculty of Biotechnology, Jeju National University)
Publication Information
Journal of Plant Biotechnology / v.44, no.3, 2017 , pp. 220-228 More about this Journal
Abstract
Many crops including cereals, tuber crops, feeds, and turf grasses are often damaged by various environmental stresses such as drought, salt, cold, and high temperature, causing the reduction of their productivity. Plants are sessile and cannot escape from environmental stresses. Thus, plants evolve in the direction of overcoming the environmental stresses. Some plant genes such as ARF, ABI3, NAC, HSF, and WRKY are known to respond to environmental stresses as they transcriptionally regulate the stress response pathways. For example, the OsWRKY76 gene contributes to the enhanced resistance to low temperatures and pathogenic infections. The AtWRKY28 also plays a role in environmental stresses. Zoysiagrass (Zoysia japonica Steud.) is popularly grown for gardens and golf courses. However, the function of the WRKY gene, another environmental stress-related gene, is not known in zoysiagrass. In this study, the ZjWRKY3 and ZjWRKY7 genes with one shared WRKY domain have been isolated in zoysiagrass. The expression of these genes increased in response to low temperature, drought, and salt stresses. Furthermore, the infection of the brown patch-causing Rhozoctonia solani induced the expression of ZjWRKY3 and ZjWRKY7. The corresponding proteins bind to the W-box of the Zjchi promoter, possibly regulating their transcriptions. The researchers suggest that the ZjWRKY3 and ZjWRKY7 genes transcriptionally regulate abiotic and biotic stress related downstream genes.
Keywords
Stress; Biotic stress; WRKY; Transcription factor; Zoysiagrass;
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