• Korean Journal of Ecology and Environment
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• v.55 no.4
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• pp.274-284
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• 2022

Through sample-size-based rarefaction analyses, we tried to suggest the appropriate degree of sample concentration and sub-sample extraction, as a way to estimate more accurate zooplankton species diversity when assessing biodiversity. When we collected zooplankton from three reservoirs with different environmental characteristics, the estimated species richness (S) and Shannon's H' values showed different changing patterns according to the amount of sub-sample extracted from the whole sample by reservoir. However, consequently, their zooplankton diversity indices were estimated the highest values when analyzed by extracting the largest amount of sub-sample. As a result of rarefaction analysis about sample coverage, in the case of deep eutrophic reservoir (Juam) with high zooplankton species and individual numbers, it was analyzed that 99.8% of the whole samples were represented by only 1 mL of sub-sample based on 100 mL of concentrated samples. On the other hand, in Soyang reservoir, which showed very small species and individual numbers, a relatively low representation at 97% when 10 mL of sub-sample was extracted from the same amount of concentrated sample. As such, the representation of sub-sample for the whole zooplankton sample varies depending on the individual density in the sample collected from the field. If the degree of concentration of samples and the amount of sub-sample extraction are adjusted according to the collected individual density, it is believed that errors that occur when comparing the number of species and diversity indices among different water bodies can be minimized.

• Analytical Science and Technology
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• v.14 no.1
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• pp.64-71
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• 2001

In this paper, the formula of lower limit of detection considering the measuring time of sample and background was derived using the basic concepts of lower limit of detection. Among parameters affecting the determination of MDA value, the MDA values were calculated with variation of amount of sample and measuring time of sample and background. The results of adequate division between the measuring time of sample and that of background studied in this research make it possible to evaluate confidence limits on the radio analytical results in the environmental sample.

• Journal of Radiation Protection and Research
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• v.20 no.1
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• pp.8-15
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• 1995

An estimate of a change in radioactivity's circumstances around the nuclear power plant is validated with the results of the radioactivity measurements are compared. In this study, to further enhance the reliability of the results obtained from the environmental radioactivity measurements and analysis around the nuclear power plants that have been carried out up to the present. In the korea standard, there is the technical analysis guide for general stable chemical element's, but there is not the technical analysis guide for the radionuclei. therefore the environmental sample collection, the pretreatment of the sample and radionuclide analysis in the sample, the result's of the environmental radioactivity measurements by each organization, etc. are different. It is not sufficient for the database to forecasting a change in radioactivity's circumstances. A comparative study of collection and pretreatment techniques for the soil sample, the results by comparison, the method of minimizing the relative error are proposed. At one side of sample collection, there are going to considered that the surroundings of sample collection like the lay of the land, the provision of the selection standard for the area and pathway of radionuclide adhesion, the coherence of sample collection, etc.. at another side of pretreatment of the sample and measurement in the case of soil sample, how to do homogeneously the soil particle size and the standard tools, i.e. kinds of meshes, must to be selected.

• Journal of Korean Society for Atmospheric Environment
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• v.22 no.5
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• pp.712-718
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• 2006

In order to investigate the analytical uncertainties associated with sampling and analysis of major greenhouse gaseous pollutants(carbon dioxide and methane), we attempted to quantify their adsorptive loss due to the contact with the container wall(such as Tedlar bag and vial). Using the GC/FID method, some basic experimental parameters(such as reproducibility and method detection limit) have been evaluated as part of the essential QA/QC The reproducibilities of carbon dioxide and methane were estimated as 2.02 and 0.2%, respectively. In addition, method detection limits were measured as 0.61 and 0.06 ng, respectively. A test of sample loss rate has also been made for Tedlar bag and vial by assessing the absolute amount of sample loss on the wall. By transferring the samples contained in Tedlar bag to various sizes of Tedlar bags, we measured differences in the absolute loss quantity due to such transfer. In addition, we also examined such loss mechanism as a function of elapsed time and light penetration rate for vial. As results, carbon dioxide and methane have shown about 2% of sample loss due to such contact. It is also noticed that the amount of loss with vial surface is lower than that of Tedlar bag. Therefore, field collection of greenhouse gases using various container types should be made more cautiously to minimize the possibility of sample loss and bias related to such loss.

• Journal of Korean Society on Water Environment
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• v.31 no.2
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• pp.159-165
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• 2015

The organic matter sources of phytoplankton and related environmental factors influencing algal bloom in Paldang reservoir were studied using nitrogen and carbon isotope ratio(${\delta}^{15}N$, ${\delta}^{13}C$). Phytoplankton samples for stable isotope analysis were collected from four points in reservoir using a plankton net. Physicochemical water quality, algal taxa and hydrological data were collected from published monitoring material. Phytoplankton samples were analyzed by IRMS. CN ratio of each sample was very similar to that of phytoplankton from literature cited. ${\delta}^{15}N$ of each sample was decreased during July. Mixing and dilution of nitrogen sources due to increment of influx by concentrated rainfall were considered as the main reason for the decline of ${\delta}^{15}N$. Based on analyzed ${\delta}^{15}N$ value of each sample, nitrogen source of Bughan river sample was presumed to come from soil. The nitrogen sources of Namhan river and Kyeongan stream samples seemed to be sewage or animal waste. Low ${\delta}^{15}N$ value in August (2012) seemed to be influenced by isotope fractionation due to the blooming of nitrogen-fixation blue-green algae (Anabaena spp.). Variation in ${\delta}^{15}N$ values particularly by blue-green algal bloom was considered the important factor for estimating the organic matter sources of phytoplankton.

• Environmental Mutagens and Carcinogens
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• v.15 no.2
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• pp.106-114
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• 1995

A method was developed to detect total mutagenicity of fried fish for S. typhimurium TA98, using Ames assay. Method described herein circumvented problems associated with the sample preparation for Ames assay, i.e., a multi-purification step of sample and interference with solvent residuals. Experiment A, the best method developed in the present study, consisted of two important steps: pH adjustment of the aqueous sample solution from fried fish samples to remove impurities, and simultaneous distillation extraction (SDE) for partially purified samples to remove volatile compounds from solvents. The procedure and results were described as below. Fillet of gizzard shad (Konosirus punctatus) fish sample fried for 10 min each side on the temperature-controlled fry-pan (210$\circ$C) was homogenized in an aqueous acidic solution (pH 2) with a homogenizer, followed by filtration through Celite. The tiltrate (pH 2), removed some impurities by extraction with chloroform:methanol (2:1, v/v) mixture, was adjusted pH to 10 and then centrifuged to remove precipitate. The ethylacetate extract from the tiltrate of pH 10 was rotoevaporated and purified by SDE apparatus for 2 hours. Experiment A revealed significantly higher revertants (1928 per 25 g fried sample) than other Experiment (B, C, or D) tested. Experiment A gave good results in the mutagenicity test of fried fish sample with few purification steps using only 25 g fried sample and 650 ml of solvents; and thus this method could be a useful tool for the screening the mutagenicity or antimutagenicity of other foods as well.

• Analytical Science and Technology
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• v.33 no.6
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• pp.274-284
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• 2020

This study described the analytical method for simultaneous determination of 89Sr and 90Sr in liquid sample using automated separation system. Radiostrontium in 0.5 kg of liquid sample was concentrated as SrCO3 to reduce the volume of sample, and purified from the sample using Sr-resin 2 mL (BV, Bed volume). The behavior of Sr and interferences such as Ba, Ca and Y were estimated with various flow rate ranging from 1 to 4 mL min-1. The detailed procedure for the purification of Sr on Sr-resin was presented. The purified radiostronitum was measured in Cerenkov mode and then measured in Scintillation mode by mixing scintillation cocktail. The measured value in both modes were used to calculate the activity of 89Sr and 90Sr. The performance tests were carried out the lab-control-sample having various activity ratio of between 89Sr and 90Sr. The recovery of Sr was ranged from 68 to 94 %. The relative bias of 89Sr activity was ranged from -5 to 20 %, and it was ranged from -10 to 10 % for 90Sr.

• Environmental Sciences Bulletin of The Korean Environmental Sciences Society
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• v.4 no.4
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• pp.215-220
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• 2000

This study on the incineration ash and sintering pellet from sewage sludge was carried out to evaluate the possibility of recycling in sewage sludge disposal. The samples used in this experiment were raw sewage sludge, its incineration ash, and sintering pellet. The characteristics of the three sample types were identified using X-ray diffraction(XRD), X-ray fluorescence(XRF), atomic absorption spectroscopy(AAS), and inductively coupled plasma spectroscopy(ICP). The chemical compositions of all three sample types were similar in their clay component, however, the sewage sludge had a high content of organic materials. From an analysis of the mineral content of the three sample types, the conversion of their compositions was found to be relative to temperature. Accordingly, incineration ash and sintering pellet both have potential for use as a functional construction material.

• Journal of Radiation Protection and Research
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• v.26 no.2
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• pp.51-58
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• 2001

In the low level radioactivity measurement, such as environmental radioactivity, there were used commonly cylindrical and Marinelli type beakers by means of measurement container. If there are differences in the matrix density or sample height between standard source and sample, it must be determined full energy peak efficiency considering self absorption effect. In this paper, we compared measured efficiency with calculated full energy peak efficiencies in the HPGe detector using the Monte Carlo method. For cylindrical container, we calculated the variation of the efficiency with sample height. Also, we calculated the variation of the detection efficiency with apparent density in the cylindrical and Marinelli container. It was seen that it need to be corrected for self absorption in the energy range of below 1000keV. Also, in order to verify the validity of calculation, we compared the calculated value with reference value using NIST SRM 4353 reference soil.

• Proceedings of the Korean Geotechical Society Conference
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• 2009.09a
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• pp.1141-1146
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• 2009

The compressibility of clay has been expressed e-log p' graph. In natural clay, e-log p' graph are changed by deposition condition and chemical cementation as well as Atterberg limits, whereas in reconstituted clay, it is generally known that e-log p’ curve is varied with Atterberg limits. However, e-log p' graph is possible to change according to the reconstituting methods and test conditions. In this study, consolidation tests are performed as various test condition for reconstituted Busan clay. Test results show that the relationship e/$e_L$ and log p' is almost constant with $e_L$. And the compression index obtained from slurry method sample is larger than one obtained from kneading method sample. Intrinsic compression line (ICL) of Busan clay is identical with ICL suggested by Burland.