• Journal of Environmental Science International
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• v.33 no.2
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• pp.187-191
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• 2024

This study aimed to investigate the effect of treating environmental purification insect larvae to pig manure on crude ash contents and ammonia production. The experiment set up consisted go two groups: 1 kg of each 3^rd instar TM (Tenebrio molitor) and 3^rd instar PBS (Protaetia brevitarsis seulensis) larvae in Experiment 1 or 3^rd and 4^th instar of HI (Hermetia illucens L.) larvae in Experiment 2 were treated with 5 kg of pig manure. In Experiment 1, the crude ash content was higher in TM larvae-treated pig manure at days 0 and 5 (p>0.05), but was similar to that in PBS larvae-treated pig manure over (p>0.05). Ammonia production was observed at day 0 of TM and PBS larvae-treated pig manure (p<0.05), but did not occur thereafter. For Experiment 2, there was significant difference in crude ash content of 3^th and 4^th instar HI larvae-treated pig manure on day 15. Additionally, ammonia production was found in 3^th and 4^th instar HI larvae-treated pig manure at days 0 and 5, but did not continue over time. In conclusion, treating TM, PBS and HI to pig manure changed the crude ash contents and reduced ammonia through the ability to decompose pig manure. Thus, environmental impact can be minimized using environmental purification insect larvae.

• Korean Journal of Environmental Biology
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• v.37 no.4
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• pp.585-591
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• 2019

Most insect-resistant LMOs have been produced by applying Cry and Vip3Aa proteins. Vip3Aa protein is activated during the vegetative stage of Bacillus thuringensis (Bt) and the inhibitory activity of the Vip3Aa protein against pathogenic attacks from lepidopteran insect species is well known. However, a risk assessment of the Vip3Aa protein compared to the Cry protein has not been conducted in South Korea. This study demonstrates a possible risk assessment method for Vip3Aa protein against honeybees (Apis mellifera). For the risk assessment of the protein, we purified the recombinant Vip3Aa protein in Escherichia coli. The survival rate and symptoms of general intoxication of 4 months honeybees were measured after Vip3Aa exposure. These results indicated that there was no significant difference in the survival rate and the symptom between Vip3Aa and the control buffer. In this study, we established standard methods of Vip3Aa protein purification and oral adult toxicity test using A. mellifera as an LMO risk assessment technique for preserving the natural ecosystem of South Korea.

• Journal of Korean Society of Rural Planning
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• v.21 no.4
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• pp.45-53
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• 2015

Agriculture, rural landscapes are accompanied by a variety of environmental issues. Therefore, it is necessary to study on biodiversity and ecosystem services. Horticulture complex is low groundwater recharge function, it can be evaluated as a facility that biodiversity is impaired. The ecosystem services in agricultural landscapes were obtained 19 kinds of functions. Experts survey Groundwater recharge function (4.13) teeth chapter higher, Water storage (4.05), Amphibian & Reptile habitat (3.96), Aquatic insect habitat (3.92), Flood control (3.87), Water purification (3.86), Avian habitat (3.76 ), Creating landscape (3.74), Vegetation diversity (3.71), Experience, Education (3.69), Biological control (3.48), Fishery habitat (3.42), Climate regulation (3.30), Mammal habitat (3.30), Air quality regulation (3.25 ), Mainenance of genetic diversity (3.25), were analyzed in order Rest area (3.14). Improving capabilities in the Detention Pond, Wetland, Green space, Corridor, Non-Chemical, Program development, Green spaces, Rainwater storage facilities, Water cycle system, Surface water storage facilities, Infiltration trench, Water purification facilities, Permeable pavement. Environmentally friendly, and to contribute to sustainable agricultural development through ecological planning.

• Biotechnology and Bioprocess Engineering:BBE
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• v.11 no.5
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• pp.442-448
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• 2006

Human lactoferrin (hLf) is an iron-binding glycoprotein that has been considered to play many biological roles in the human, including the stimulation of the immune system, antimicrobial and anti-inflammatory effects, and regulation of iron absorption. We generated transgenic Siberian ginseng (Acanthopanax senticosus) cell cultures producing a functional hLf protein using the signal peptide sequence from the endoplasmic reticulum and driven by an oxidative stress-inducible SWPA2 promoter which is highly expressed in plant cell cultures. The production of hLf increased proportionally to cell growth and showed a maximal level (up to 3.6% of total soluble protein) at the stationary phase in suspension cultures. Full-length hLf protein was identified by immunoblot analysis in transgenic cell cultures of Siberian ginseng. Recombinant hLf (rhLf) was purified from suspension cells of Siberian ginseng by ammonium sulfate precipitation, cation-exchange and gel filtration chromatography. N-terminal sequences of rhLf were identical to native hLf (nhLf). The overall monosaccharide composition of rhLf showed the presence of plant specific xylose while sialic acid is absent. Antibacterial activity of purified rhLf was higher than that of nhLf. Taken together, we anticipate that medicinal Siberian ginseng cultured cells, as demonstrated by this study, will be a biotechnologically useful source for commercial production of functional hLf not requiring further purification.

• Journal of Wetlands Research
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• v.16 no.4
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• pp.319-325
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• 2014

A lot of Pond Wetland (Palustrine Wetland) are distributed in agricultural landscapes, Korea. These wetlands are evaluated as important resources for conservation of biodiversity. However, the study of Pond Wetland is rarely conducted except type classification in Korea. In this study, the function of pond wetland as ecosystem services is studied for conservation and utilization of wetland. Research was conducted in three steps; functionality analysis, functionality derivation, and importance analysis. A total of 22 features were derived by analyzing the previous studies. As a result of expert survey, 4 features were selected by each in the field of biological, environmental, and socio-culture among the 22 features. Importance was calculated by conducting a cross-functional features using Analytic Hierarchy Process (AHP). Finally, 10 ecosystem service functions of pond wetland were derived through expert survey; (1) Water Storage & Irrigation, (2) Vegetation Diversity, (3) Amphibian & Reptile Habitat, (4) Water Purification, (5) Aquatic Insect Habitat, (6) Nutriments Control was derived, (7) Groundwater Recharge, (8) Fishery Habitat, (9) Eco-Experience & Education, and (10) Aesthetic landscape. It is expected that this study is able to utilize in evaluation of pond wetlands ecosystem services by further study on analysis of functional importance and economic value index.

• Journal of Life Science
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• v.31 no.12
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• pp.1094-1099
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• 2021

Hermetia illucens (Black soldier fly) is attracting attention as an environmental purification insect because it can supply a wide range of by-products of the agricultural food industry. Also, it has a potential feed for fish, birds, and pets due to a short life cycle and excellent nutritional components. Several pharmacological effects, including antimicrobial, of H. illucens have been reported. However, no study has focused on antiobesity effects of ethanol extract of H. illucens. In this study, we aimed to assess the anti-obesity effects of ethanol extract of H. illucens larvae (HIE) through inhibition of differentiation of 3T3-L1 preadipocytes into adipocytes. The amount of lipid accumulated in adipocytes was measured by oil red-O staining, and the inhibitory effect on adipogenesis was confirmed. The expression levels of factors related to adipocyte differentiation and fat synthesis were determined using Western blot analysis. Lipid droplet formation in adipocytes was remarkably inhibited by HIE. In addition, treatment with 400 ㎍/ml of HIE significantly reduced the expression levels of peroxisome proliferator-activated receptor γ and CCAAT/enhancer-binding protein α-transcription factors involved in adipocyte differentiation. Therefore, the results of this study indicate that HIE is a potential anti-obesity agent because it inhibits adipocyte differentiation.

• Journal of Wetlands Research
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• v.19 no.4
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• pp.533-541
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• 2017

Agricultural landscape has many ecosystem service functions. However, the development of the horticulture complex has no consideration for environmental conservation. Therefore, we analyzed the priorities of ecosystem service functions required for the composition. The study was conducted in three stages. As a result of the first survey, 17 functions were selected to be improved. In the second survey, 12 functions were selected excluding 5 functions. Finally, 1. Measures for water purification, 2. Groundwater recharge plan, 3. Surface water storage space, 4. Flood control measures, 5. Vegetation diversity space, 6. Carbon emission reduction plan, 7. Aquatic insect habitat space, 8. Amphibian reptiles 9. Landscape and Waste Management, 10. Bird Species Space, 11. Heat Island Mitigation Plan, 12. Experience / Ecological Education Plan. We proposed the structure, capacity, flow rate, arrangement and form of the water treatment facility to improve water quality by improving the function. We proposed a reservoir space of 7-10% for groundwater recharge. The development of reservoir and storage facilities suitable for the Korean situation is suggested for the surface water storage and flood control measures. And proposed to secure a green space for the climate cycle. Proposed habitat and nutrient discharge management for biodiversity. We propose green area development and wetland development to improve the landscape, and put into the facilities for experiential education. The results of the research can be utilized for the development and improvement of the horticultural complex.