• Title/Summary/Keyword: Environmental performance

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Development of a Residue Analysis Method for Metamifop in Paddy Water, Soil, and Rice with HPLC (HPLC를 이용한 농업용수, 논토양, 및 현미 중 metamifop의 잔류분석법 개발)

  • Park, Hee-Woon;Moon, Joon-Kwan
    • The Korean Journal of Pesticide Science
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    • v.21 no.1
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    • pp.68-74
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    • 2017
  • An analytical method for detecting metamifop residue in paddy water, soil, and rice with high performance liquid chromatography (HPLC) was developed. Water was extracted with ethyl acetate before analyzing by HPLC. Soil residues were extracted with acetone under acidic condition and after purifying with $Extrelut^{(R)}$ NT, and silica SPE, the residue was analyzed by HPLC. For residue analysis in rice, the procedure involved extraction with acetone, purification with $Extrelut^{(R)}$ NT, partitioning between acetonitrile/hexane, purification with silica SPE cartridge, and analysis by HPLC. The limit of detection (LOD) was 1.0 ng, limit of quantitation (LOQ) was 3.0 ng, and method limit of quantitation (MLOQ) were 0.001 mg/L for paddy water, 0.01 mg/kg for rice and soil, respectively. Standard calibration curve shows linearity from 0.05 mg/kg to 5.0 mg/kg ($R^2=0.9999$). The recoveries in fortified paddy water were $91.3{\pm}3.5%$ (0.01 mg/L level) and $93.2{\pm}6.3%$ (0.05 mg/L level). The recoveries in fortified paddy soils were $92.5{\pm}4.0%$ (0.1 mg/kg level) and $92.7{\pm}4.0%$ (0.5 mg/kg level) in soil A, while, $102.3{\pm}4.4%$ (0.1 mg/kg level) and $98.9{\pm}7.9%$ (0.5 mg/kg level) in soil B, respectively. The recoveries in fortified rice were $93.0{\pm}6.9%$ (0.1 mg/kg level) and $85.0{\pm}3.5%$ (0.5 mg/kg level). This method was proved to be effective and can be used to determine the metamifop residue in paddy water, paddy soil, and rice.

Study on the estimation of environmental effects on milk yield in Holstein (Holstein종(種)의 유량(乳量)에 영향(影響)을 미치는 환경효과(環境效果) 추정(推定)에 관한 연구(硏究))

  • Yun, Doo Hag;Choi, Kwang Soo
    • Current Research on Agriculture and Life Sciences
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    • v.9
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    • pp.37-49
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    • 1991
  • This study was conducted to estimate the effects of year, age of dam at calving, farm and lactation period on milk yield with the data of 4,008 cows' records which were collected at 32 farms by Korea Animal Improvement Association from 1985 to 1989. The results obtained in this study are summarized as follows: 1. The average performance of the dairy cattle in the study were $5,959.23{\pm}2,113.03kg$ in actual milk yield, $49.19{\pm}22.77$ months in age of dam at calving, $27.11{\pm}5.13$ months in age at first calving and $255.11{\pm}79.68$ days in lactation period. 2. The percentages of variance component for different sources were 29.39% for the residuals, 1.91% for years, 4.86% for age at calving, 8.89% for farms and 54.94% for lactation period. 3. The overall mean of least-square estimate on the milk yield was 6,229.31kg. In the effects of year, the least-square means of milk yield were estimated 6,000.76kg in 1985-1987, 6,028.11kg in 1988 and 6,659.07kg in 1989. 4. The least-square means of calving age on the milk yield were estimated 5,456.01kg in less than 24 months, 6,565.48kg in 61-66 months which were the highest least-square means. This effects were gradually increased until the 61-66months and gradually decreased after the 61-66months, with highly significant differences among different months of age at calving(p<0.01). 5. In the effects of farm, the least-square means of milk yield were estimated 4,959.50 kg in the lowest farm and 7,497.07kg in the highest farm. Among the milk yield of each farm the effects showed highly significant difference(p<0.01). 6. The least-square means of milk yield in the effects of lactation period were gradually increased with the lapse of the lactation period. Among the lactation period the effects showed highly significant difference(p<0.01).

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The Effect of Vision Sharing at Social Enterprise on Organizational Socialization - Focusing on Mediation Effects of Organizational Health - (사회적기업 종사자의 비전공유가 조직사회화에 미치는 영향 -조직건강을 매개로-)

  • Cheon, Han-Seul;Cho, Young-Bohk;Lee, Na-Young
    • Management & Information Systems Review
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    • v.37 no.1
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    • pp.75-101
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    • 2018
  • Social enterprise in Korea has faced with many problems such as small size, management capability, lack of technology and weak ability to obtain resources despite its quantitative growth, raising concern over sustainability of social enterprises. Despite such tough environment, unique feature of social enterprise, differentiated from commercial enterprise is that it has clear social mission. In addition, social enterprise has the organizational feature in that vulnerable social group of workers coexists with ordinary workers, and plays a role of helping independence of vulnerable social group. Due to this feature, successful organizational socialization of members in social enterprise is a very important feature. Based on assumption that social mission of social enterprise can be utilized as the unique competitiveness of social enterprise through vision-sharing in the organization, and may give positive effects on successful organizational socialization of organization members, this study aims to conduct empirical research on relationship between vision-sharing and organizational socialization and to explore mediation effects of organizational health as organizational environmental element in relationship between vision sharing and organizational socialization. This study was conducted on 156 employees working at social enterprises. As a result of study, first, vision sharing is found to have positive effects on organizational socialization at social enterprises. Second, vision sharing in social enterprise has positive effects on organizational health. Third, vitality and community-oriented in social enterprise are found to have mediation effects among lower elements of organizational health in relationship between vision sharing and organizational socialization. In conclusion, it is confirmed that the more visions of organization are shared, the more members recognize their organization healthy, resulting in successful organizational socialization. This study is meaningful in that it presents the plans for successful organizational socialization of members of social enterprise including vulnerable groups and that it is the empirical study on plans of social enterprise on human resource management.

The Work and Job Satisfaction of Paramedics in the Emergency Room of University Hospitals (대학병원 응급실 내 1급 응급구조사의 업무와 직무만족도)

  • Lee, Ok-Hee
    • The Korean Journal of Emergency Medical Services
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    • v.15 no.1
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    • pp.47-63
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    • 2011
  • Purpose : This research is to examine the work and job satisfaction of paramedics in the emergency room of university hospitals. This research is done to provide basic data needed for establishing work realms of paramedics in hospitals and to enhance their degree of satisfaction. Methods : Research questionnaire survey was conducted on 141 paramedics working in the emergency room of 32 university hospitals from August 24, 2010 to September 30, 2010 through direct visits and telephone interviews or email to explain the purpose of this research and assurance of confidentiality of responses on the questionnaires. As the tool for the degree of job satisfaction, 'The Index of Work Satisfaction' developed by Slavitt, et al(1978) and revised and supplemented by Soon-shim Kim and Hye-ran Kwon(2002) was used. The collected data were analyzed by evaluating frequency, percentage, mean, standard deviation, t-test and ANOVA, Cronbach's $\alpha$ by using SPSS WIN 18.0 program. Results : 1. Investigating the work and role of paramedics in the emergency room of university hospitals, electrocardiogram(EKG) was found to be highest with $\alpha$ was widely used with the rate of patient evaluation and test area. In the medical treatment for patients area, cardiopulmonary resuscitation(CPR) with 95%(134 persons) and ventilation assistance through ambu bagging(BVM) with 95%(134 persons) were found to be high. $\alpha$ were performed. In the role within the hospital and other areas, a member of CPR team in the hospital accounted for 78%(110 persons). 2. In the measurements of the job satisfaction of paramedics working at university hospitals, the total mean score was 2.91. The mean score in each question area indicated: section on job 3.48, autonomy 3.05, interaction 3.01, organizational demand 2.85, working conditions 2.67, salaries 2.40. This result obviously demonstrates the work of paramedics itself was most satisfied but the salaries were most dissatisfied. 3. In the measurements of the job satisfaction of paramedics working at university hospitals, job satisfaction based on the general characteristics showed significant difference in age (F=6.547, p=.002), gender (F=4.436, p=.000) marital status (F=-3.270, p= .001), religion (F=2.041, p= .043), motive for application (F=3.603, p= .015), and salary (F=6.658, p= .000). 대학병원 응급실 내 1급 응급구조사의 업무와 직무만족도 The Journal of the Korean Society of Emergency Medical Technology Vol. 15 (1) 63 4. In the measurements of the job satisfaction of paramedics working at university hospitals, job satisfaction based on the working environmental characteristics showed significant difference in total number of paramedics (F=3.779, p= .012), form of employment (F=5.601, p= .001), existence or non-existence of intention to change jobs (F=-4.037, p= .000). Conclusion : The work of paramedics in the emergency room of university hospitals consists of lots of treatment processes after specialized diagnosis and performance of professionally subdivided works. However, current legislation does not reflect such circumstances to which paramedics are exposed; thus, it should be considered for further revision and modification. The degree of job satisfaction of paramedics in the emergency room of university hospitals was high but low in salaries and working conditions were the weak points. The measures to enhance their degree of job satisfaction should be taken though improvement of labor conditions such as consideration of the rate of increase in salaries, compensation for overtime work, providing rest areas, improvement of current employment system, and conversion of temporary employees into regular employees.

Organizational Factors Facilitating the Internationalization of Korean Franchising Companies (해외진출 국내 프랜차이즈기업의 조직특성)

  • Lim, Young-Kun;Lee, Dong-Whuy;Kim, Hee-Jung
    • Journal of Global Scholars of Marketing Science
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    • v.19 no.2
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    • pp.40-52
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    • 2009
  • Franchising is one of the fastest growing types of business. It is already popular and well-known in the U.S., and has been growing in many other countries including Korea. Furthermore, many Korean franchising companies have expanded their business overseas actively. According to the data by the Ministry of Industry and Resource, 82 companies out of a sample of 500 franchising companies are already operating in many foreign countries and 48% of them have started their foreign business since 2006. This clearly indicates the fast growing current trend of foreign operation by Korean franchising companies. In spite of the fast growing trend of foreign expansion in the industry, academic research on internationalization of franchising companies is extremely difficult to find. Accordingly, academic research on the issue is necessary and urgent in Korea. Among the various research questions on internationalization of franchising business, this study intends to investigate the difference in organizational factors between the franchising companies doing foreign operation and those doing business only domestically. More specifically, this research has the following purposes. First, considering the lack of theoretical basis of previous studies, resource-based theory and agency theory are employed as the theoretical bases. Second, this study explains the difference in internationalization based on organizational factors such as company size, history and growth rate. Third, the five hypotheses regarding the difference in organizational factors are presented and tested empirically, which is the first attempt in the area of this topic. Finally, the study attempts to clarify the conflicting implications among theories regarding some organizational factos such as growth rate. As the theoretical background, resource-based theory and agency theory are discussed. According to resource-based theory, a firm can grow continuously when it has competence and resource, and also the ability to develop them. The competence and resource can include capital, human resource, management skill, market information, ability to manage risk, etc. Meanwhile, agency theory views the relationship between franchisor and franchisee as an agency relationship. In agency theory, bonding capability and monitoring capability are the two key factors which promote internationalization of franchising companies. Based on the two theories, a conceptual model is designed. The model consists of two groups of variables. One is organizational factors including size, history, growth rate, price bonding and geographic dispersion. The other is whether a franchising company is operating overseas or not. We developed the following five research hypotheses basically describing the relationship between organizational factors and internationalization of franchising companies. H1: The size of franchising companies operating overseas is larger than that of franchising companies operating domestically. H2: The history of franchising companies operating overseas is longer than that of franchising companies operating domestically. H3: The growth rate of franchising companies operating overseas is higher than that of franchising companies operating domestically. H4: The price bonding of franchising companies operating overseas is higher than that of franchising companies operating domestically. H5: The geographic dispersion of franchising companies operating overseas is wider than that of franchising companies operating domestically. Data for the analyses are obtained from 2005 Korea Franchise Survey data co-generated by Ministry of Industry and Resource, GS1 Korea, and Korea Franchise Association. Out of 2,804 population companies, 2,489 companies are excluded for various reasons and 315 companies are selected as the final sample. Prior to hypotheses tests, validity and reliability of the measures of size, history, growth rate and price bonding are examined for further analyses. Geographic dispersion is not validated since it is measured using nominal data. A series of independent sample T-tests is used to find out whether there exists any significant difference between the companies internationalized and those operating only domestically for each organizational factor. Among the five factors, size and geographic dispersion show significant difference, growth rate and price bonding do not reveal any difference and, finally, history factor shows conflicting results in the difference depending on how to measure it.

    shows the summary statistics for hypotheses testing. In conclusion, the results show that the size and history, which are the key variables in resource-based theory, have a significant relationship with internationalization and that geographic area, which belongs to agency theory, also has a strong relationship with internationalization. The results support the findings of extant research and, therefore, prove the usefulness of resource-based theory and agency theory in explaining internationalization of franchising companies. However, growth rate and price-bonding do not show a clear difference between the two types of companies. Accordingly, these two factors need further attention in the future research. Although this study shows meaningful findings theoretically and practically, it has several limitations. First, only organizational factors are considered even if there are various environmental factors influencing franchising firm's internationalization. Second, only being internationalized or not is considered. That is, modes of entry and the size of foreign operations are not included in the study. Third, internationalization strategy is often determined based on the desire for business expansion and higher profitability and egoistical reasons of the CEOs. However, this type of factors belonging to behavioral science is not discussed in the study. Finally, organizational ecology perspective is usefully applicable in explaining the survival and performance of internationally operating companies. Accordingly, research propositions based on this perspective need to be developed and tested.

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  • Semi-pilot Scaled Biofilter Treatment of Malodorous Waste Air Containing Hydrogen Sulfide and Ammonia: 2. Performance of Biofilter Packed with Media Inoculated with a Consortium of Separated Microbes (황화수소와 암모니아를 함유한 악취폐가스의 세미파일럿 규모 바이오필터 처리: 2. 분리 미생물들을 접종한 담체를 충전한 바이오필터 운전)

    • Lim, Kwang-Hee
      • Korean Chemical Engineering Research
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      • v.52 no.2
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      • pp.240-246
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      • 2014
    • A semi-pilot biofilter inoculated with the microbes consortium of Bacillus cereus DAH-1056 and Arthrobacter sp. KDE-0311 was operated under various operating conditions in order to treat malodorous waste air containing both hydrogen sulfide and ammonia. When both hydrogen sulfide and ammonia contained in malodorous waste air were treated simultaneously by semi-pilot biofilter inoculated with Thiobacillus sp. IW and return-sludge, the removal efficiencies of hydrogen sulfide and ammonia were ca. 80% and ca. 50%, respectively. On the other hand, in this study, the removal efficiencies of hydrogen sulfide and ammonia were ca. 90% and ca. 60%, respectively. Therefore, the removal efficiencies of hydrogen sulfide and ammonia were enhanced by ca. 13% and 20%, respectively, compared to the semipilot biofilter inoculated with Thiobacillus sp. IW and return-sludge. In addition, in this study, the maximum elimination capacities of hydrogen sulfide and ammonia were enhanced by ca. 15% ($8g/m^3/h$) and 10~17% ($3{\sim}5g/m^3/h$), respectively. In this study, it was observed either that in case of even a same inlet load of hydrogen sulfide, a higher concentration of hydrogen sulfide causes more difficulties in treating ammonia containing in waste air than a lower one, or that in case of even a same inlet load of ammonia, a lower concentration of ammonia results in higher removal efficienciy and elimination capacity than a higher one. Even though hydrogen sulfide and ammonia were treated simultaneously by a biofilter in this study, the maximum elimination capacity of hydrogen sulfide in this study exceeded or was similar to that in previous study of biofilter treating only hydrogen sulfide. In addition, this study showed the higher maximum elimination capacity of ammonia than other previous investigation of biofilter treating hydrogen sulfide and ammonia simultaneously.

    Clinical Charicteristics of Primary Lung Cancer Patients in a Tertiary Hospital (3차 의료기관에서 진단된 원발성 폐암의 임상적 특성)

    • Ryu, Jeong-Seon;Lee, Hun-Jae;Leem, Jong-Han;Kim, Lucia;Lee, Kyung-Hee;Cho, Jae-Hwa;Yoon, Young-Han;Kwak, Seung-Min;Lee, Hong-Lyeol;Kim, Kwang-Ho;Loh, John-Kyu;Jung, Soo-Kyung
      • Tuberculosis and Respiratory Diseases
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      • v.60 no.3
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      • pp.321-329
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      • 2006
    • Background : To evaluate the clinical characteristics of lung cancer patients in Korea, where there is a higher number of smokers than in Western countries. Methods : A retrospective study was performed on 1655 lung cancer patients, who were diagnosed at a university hospital between September 1996 and August 2005. Age, gender, cell types and clinical stage were analysed. Of 941 patients, who responded to a questionnaire at the time of diagnosis, the smoking habits, occupational history, family history of lung cancer in the first-degree relatives, coexisting diseases (diabetes mellitus and cardiovascular disease), body weight loss, ECOG performance status and presenting symptoms, were examined prospectively. In addition, coexisting diseases including chronic obstructive pulmonary disease, idiopathic pulmonary fibrosis and active pulmonary tuberculosis were evaluated. Results : Of the 1655 patients, the male to females ratio was 3.6. Squamous cell carcinoma was the most common cancer whereas adenocarcinoma was more common in lifetime nonsmokers or women. 19.9% of the patients were non smokers and 80.1% ever smokers. Since 2000, there was an increase in the incidence of adenocarcinoma with a corresponding decrease in the incidence of squamous cell carcinoma. 6.2% of patients were asymptomatic. A coincident diagnosis of chronic obstructive pulmonary disease, cardiovascular disease, diabetes mellitus, active pulmonary tuberculosis, and idiopathic pulmonary fibrosis was made in: 44.1%, 22.2%, 10.7%, 3.9%, and 1.6% of patients, respectively. A positive family history of lung cancer in the first-degree relatives was identified in 4.4% of patients. An occupational history relevant to lung cancer was identified in 12.2% of patients. Conclusion : There is a high proportion of cigarette smokers in Korean lung cancer patients. The most common cell type was squamous cell carcinoma. However, a more detailed, prospective study of the clinical characteristics will be needed to better characterize lung cancer in Korea.

    A Study on Singapore Startup Ecosystem using Regional Transformation of Isenberg(2010) (싱가포르 창업생태계 연구: Isenberg(2010) 프레임워크의 지역적 변용을 통한 질적 연구를 중심으로)

    • Kim, Soyeon;Cho, Minhyung;Rhee, Mooweon
      • Asia-Pacific Journal of Business Venturing and Entrepreneurship
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      • v.15 no.2
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      • pp.47-65
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      • 2020
    • With the era of the Fourth Industrial Revolution in sight, innovative business models utilizing new technologies are emerging, and startups are enjoying an abundance of opportunities based on the agility to respond to disruptive innovations and the opening to new technologies. However, what is most important in creating a sustainable start-up ecosystem is not the start-up itself, but the process of research-start-investment-investment-the leap to listing and big business-in order to build a virtuous circle of startups that leads to re-investment. To this end, the environment created in the hub area where start-ups were conducted is important, and these material and non-material environmental factors are described as being inclusive by the word "entrepreneurial ecosystem." This study aims to provide implications for Korea's entrepreneurial ecosystem through the study of the interaction of the elements that make up the start-up ecosystem and the relationship of ecosystem participants in Singapore. Singapore has been consistently mentioned as the top two Asian countries in assessing the start-up environment and business environment. In this process, six elements of the entrepreneurial ecosystem presented by Isenberg(2010)-policies, finance, culture, support, human resources, and market-are the best frameworks for analyzing entrepreneurial ecosystems in terms of well encompassing prior studies related to entrepreneurial ecosystem elements, and a model of regional transformation is formed focusing on some elements to suit Singapore, the target area of study. By considering that Singapore's political nature would inevitably have a huge impact on finance, Smart Nation policy was having an impact on university education related to entrepreneurship, and that the entrepreneurial networks and global connectivity formed within Singapore's start-up infrastructure had a significant impact on Singapore's start-up's performance, researches needed to look more at the factors of policy, culture and market. In addition, qualitative research of participants in the entrepreneurial ecosystem was essential to understand the internal interaction of the elements of the start-up ecosystem, so the semi-structured survey was conducted by visiting the site. As such, this study examined the status of the local entrepreneurial ecosystem based on qualitative research focused on policies, culture and market elements of Singapore's start-up ecosystem, and intended to provide implications for regulations related to start-ups, the role of universities and start-up infrastructure through comparison with Korea. This could contribute not only to the future research of the start-up ecosystem, but also to the creation of a start-up infrastructure, boosting the start-up ecosystem, and the establishment of the orientation of the start-up education in universities.

    Genome-Wide Analysis of DNA Methylation before- and after Exercise in the Thoroughbred Horse with MeDIP-Seq

    • Gim, Jeong-An;Hong, Chang Pyo;Kim, Dae-Soo;Moon, Jae-Woo;Choi, Yuri;Eo, Jungwoo;Kwon, Yun-Jeong;Lee, Ja-Rang;Jung, Yi-Deun;Bae, Jin-Han;Choi, Bong-Hwan;Ko, Junsu;Song, Sanghoon;Ahn, Kung;Ha, Hong-Seok;Yang, Young Mok;Lee, Hak-Kyo;Park, Kyung-Do;Do, Kyoung-Tag;Han, Kyudong;Yi, Joo Mi;Cha, Hee-Jae;Ayarpadikannan, Selvam;Cho, Byung-Wook;Bhak, Jong;Kim, Heui-Soo
      • Molecules and Cells
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      • v.38 no.3
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      • pp.210-220
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      • 2015
    • Athletic performance is an important criteria used for the selection of superior horses. However, little is known about exercise-related epigenetic processes in the horse. DNA methylation is a key mechanism for regulating gene expression in response to environmental changes. We carried out comparative genomic analysis of genome-wide DNA methylation profiles in the blood samples of two different thoroughbred horses before and after exercise by methylated-DNA immunoprecipitation sequencing (MeDIP-Seq). Differentially methylated regions (DMRs) in the pre-and post-exercise blood samples of superior and inferior horses were identified. Exercise altered the methylation patterns. After 30 min of exercise, 596 genes were hypomethy-lated and 715 genes were hypermethylated in the superior horse, whereas in the inferior horse, 868 genes were hypomethylated and 794 genes were hypermethylated. These genes were analyzed based on gene ontology (GO) annotations and the exercise-related pathway patterns in the two horses were compared. After exercise, gene regions related to cell division and adhesion were hypermethylated in the superior horse, whereas regions related to cell signaling and transport were hypermethylated in the inferior horse. Analysis of the distribution of methylated CpG islands confirmed the hypomethylation in the gene-body methylation regions after exercise. The methylation patterns of transposable elements also changed after exercise. Long interspersed nuclear elements (LINEs) showed abundance of DMRs. Collectively, our results serve as a basis to study exercise-based reprogramming of epigenetic traits.

    Diagnostic Performance of HPV E6/E7 mRNA and HPV DNA Assays for the Detection and Screening of Oncogenic Human Papillomavirus Infection among Woman with Cervical Lesions in China

    • Wang, Hye-young;Lee, Dongsup;Park, Sunyoung;Kim, Geehyuk;Kim, Sunghyun;Han, Lin;Yubo, Ren;Li, Yingxue;Park, Kwang Hwa;Lee, Hyeyoung
      • Asian Pacific Journal of Cancer Prevention
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      • v.16 no.17
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      • pp.7633-7640
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      • 2015
    • Background: Human papillomavirus (HPV) is the most common sexually transmitted infection worldwide and it is responsible for most cases of cervical uterine cancer. Although HPV infections of the cervix do not always progress to cancer, 90% of cervical cancer cases have been found to be associated with high risk HPV (HR-HPV) infection. HPV DNA testing is widely used, along with Papanicolaou (Pap) testing, to screen for cervical abnormalities. However, there are no data on the prevalence of genotype-specific HPV infections assessed by measuring HPV E6/E7 mRNA in women representative of the Chinese population across a broad age range. Materials and Methods: In the present study, we compared the results with the CervicGen HPV RT-qDx assay, which detects 16 HR-HPV genotypes (Alpha-9: HPV 16, 31, 33, 35, 52, and 58; Alpha-7: HPV 18, 39, 45, 51, 59, and 68; and Alpha-5, 6: HPV 53, 56, 66, and 69), and the REBA HPV-ID assay, which detects 32 HPV genotypes based on the reverse blot hybridization assay (REBA) for the detection of oncogenic HPV infection according to cytological diagnosis. We also investigated the prevalence and genotype distribution of HPV infection with a total of 324 liquid-based cytology samples collected in western Shandong province, East China. Results: The overall HPV prevalences determined by HPV DNA and HPV E6/E7 mRNA assays in this study were 79.9% (259/324) and 55.6% (180/324), respectively. Although the positivity of HPV E6/E7 mRNA expression was significantly lower than HPV DNA positivity, the HPV E6/E7 mRNA assay showed greater specificity than the HPV DNA assay (88.6% vs. 48.1%) in normal cytology samples. The prevalence of Alpha-9 (HPV 16, 31, 33, 35, 52, and 58) HPV infection among these women accounted for up to 80.3% and 76.1% of the high-grade lesions detected in the HPV mRNA and DNA tests, respectively. The HR-HPV genotype distribution, based on HPV DNA and E6/E7 mRNA expression by age group in patients with cytologically confirmed lesions, was highest in women aged 40 to 49 years (35.9% for cytologically confirmed cases, Pearson correlation r value=0.993, p<0.001) for high-grade lesions. Among the oncogenic HR-HPV genotypes for all age groups, there was little difference in the distribution of HPV genotypes between the HPV DNA (HPV -16, 53, 18, 58, and 33) and HPV E6/E7 mRNA (HPV -16, 53, 33, 58, and 18) assays. HPV 16 was the most common HPV genotype among women with high-grade lesions. Conclusions: Our results suggest that the HPV E6/E7 mRNA assay can be a sensitive and specific tool for the screening and investigation of cervical cancer. Furthermore, it may provide useful information regarding the necessity for early cervical cancer screenings and the development of additional effective HPV vaccines, such as one for HPV 53 and 58. Additionally, gaining knowledge of HPV distribution may also inform us about ecological changes in HPV after the vaccination.


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