• 대한가정학회지
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• 제37권1호
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• pp.29-44
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• 1999

The objectives of this study are (1) to identify sub-areas of the pro-environmental behavior, (2) to examine the level of the pro-environmental attitude and behavior, (3) to examine the degree of inconsistency between attitude and behavior, and (4) to investigate the reasons of the inconsistency. Data was collected from the questionnares with 299 married men and 378 married women and the deep interviews with 10 married women. The major findings are follows : (1) The pro-environmental behavior can be classified into 2 sub-areas : social-oriented area and individual-oriented area. (2) The level of pro-environmental attitude and behavior in the social-oriented area is lower than those in individual-oriented area. (3) The attitude-behavior inconsistency in the social-oriented sub-area is stronger than that in the individual-oriented sub-area. (4) The reasons of the pro-environmental attitude-behavior inconsistency are the lack of the sincere environmental consciousness and the existence of the pro-environmental behavior restriction factors.

• 한국물환경학회지
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• 제24권1호
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• pp.19-29
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• 2008

Hot air sparging is a groundwater remediation technique, in which organic contaminants volatilized into hot air from the saturated to vadose zone. In the laboratory diesel (10,000 mg TPH/kg) was spiked in contaminated saturated aquifer soil. The hot air ($34.9{\pm}2.7^{\circ}C$) was injected in intermittent (Q=1,500 mL/min, 10 minute injection and 10 minute idle) modes. We performed microcosm tests using the groundwater samples to assess TPH reductive remediation activity. For Terminal-Restriction Fragment Length Polymorphism (T-RFLP) analysis of eubacterial communities in sludge of wastewater treatment plants and soil of experiment site, the 16S rDNA was amplified by Polymerase Chain Reaction (PCR) from the sludge and the soil. The obtained 16S rDNA fragments were digested with Msp I and separated by electrophoresis gel. We found various sequence types for hot air sparging experiment with sludge soil samples that were closely related to Bacillus (149 bp, Firmicutes), Methlobacterium (149 bp, Euryarchaeotes), Pseudomonas (492 bp, ${\gamma}$-Proteobacteria), etc., in the clone library. In this study we find that TPH-water was reduced to 78.9% of the initial value in this experiment aquifer. The results of the present study suggests that T-RFLP method may be applied as a useful tool for the monitoring in the TPH contaminated soil fate of microorganisms in natural microbial community.

• 한국환경보건학회지
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• 제50권1호
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• pp.54-65
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• 2024

Background: There is growing international recognition of the need for improvements to national chemical management systems for hazardous chemicals. The European Union has recently introduced the concept of 'essential uses' as a new approach to the management hazardous chemicals by limiting their uses. Objectives: This paper examines the concept of essential uses in chemical management and how to apply it through a case study of essential use. This approach is distinct from the current chemical management system, but seeks to improve its potential benefits by effectively restricting or gradually decreasing the use of hazardous substances. Methods: The concept of essential uses was introduced by reviewing the Montreal Protocol on Substances that Deplete the Ozone Layer, Cousins's three essentiality categories applied to PFAS, restriction options assessed in the PFAS restriction proposal under REACH, and the California Safer Consumer Products regulations prioritizing 6PPD in motor vehicle tires. Based on these essential uses concepts for PFAS and 6PPD, uses of benzene were classified in accordance with the essential uses approach for products using benzene in South Korea. Results: The essential use concept is able to manage the restriction and authorization of substances of concern through essential uses and non-essential uses and the feasible substitution of uses and substances. Conclusions: If the concept and methodology of essential uses are clearly established, they can be expected to shift the national chemical management paradigm from regulating substances to limiting uses under the existing substance management system.

• 대한환경공학회지
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• 제32권4호
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• pp.369-378
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• 2010

폐기물매립장의 안정화에는 미생물이 중요한 역할을 수행한다. 폐기물매립장에서 미생물군집 변화 모니터링에 말단 제한절편다형성(Terminal Restriction Fragment Length Polymorphism; T-RFLP)법의 활용 가능성을 평가하고자 박테리아의 16S rDNA 서열에 기초한 T-RFLP법으로 4개의 폐기물매립장 내부에서 채취한 침출수의 미생물군집 구조를 조사하였다. T-RFLP법을 사용하여 해석한 침출수 내 우점 미생물군집 구조와 일반적으로 널리 사용되고 있는 16S rDNA 클론 해석법에 의한 우점 미생물군집구조는 유사하였다. 또한, T-RFLP법을 이용하여 폐기물매립장의 구조, 매립 폐기물 종류, 운영기간이 다른 폐기물매립장 침출수의 우점 미생물군집 구조가 서로 다르게 나타나는 것을 확인 할 수 있었다. 따라서 T-RFLP법을 사용하여 폐기물매립장 침출수내 미생물군집 구조를 장기적으로 모니터링 한다면 많은 비용과 시간이 소요되는 클론해석법의 반복적인 수행 없이도 비교적 간단하게 폐기물매립장의 안정화 정도를 평가할 수 있을 것으로 기대한다.

• 산업경영시스템학회지
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• 제19권39호
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• pp.81-88
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• 1996

As international commerce pattern changes toward the system of developed countries market competition, developing countries experience difficulties in international commerce market. Developed countries request their trading counterparts to obtain environment restriction. We also endure the environmental pollution due to industrialization. These environmental problems force Western Europe countries to install Environment Management System. This article presents description of development processes of ISO 14000 draft, compares ISO 9000 with 7750 requirements, and describes the common points and differences between ISO 9000 and 14000 draft.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2003년도 춘계학술대회
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• pp.177-177
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• 2003

Adult periodontitis is a chronic inflammatory disease whose etiology is not well defined. Recent studies have shown that vitamin D receptor gene has been a candidate for the susceptibility of adult periodontitis. The purpose of this study is to investigate the frequency of Taq I restriction fragment length polymorphism (RELP) in the vitamin D receptor gene in 141 periodontically healthy controls and 32 adult periodontitis patients. Taq I RFLP in the vitamin D receptor gene were detected by PCR amplification, followed by restriction enzyme digestion and 2% agarose gel electrophoresis. There were no significant difference in the distribution of Taq I RFLP between healthy controls and adult periodontitis group (P > 0.05). Thus, Taq I RFLP in the vitamin D receptor gene may not confer the susceptibility to adult periodontitis in Korean population. However, t allele distributions of this RFLP showed various frequencies among ethnic groups studied. Further studies in other ethnic groups will be required.

• Journal of Microbiology and Biotechnology
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• 제9권1호
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• pp.78-83
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• 1999

Thirty-six environmental isolates of Vibrio vulnificus obtained from seawater, sediments, and raw seafoods, and 18 clinical isolates from Vibrio septicemia patients were typed by restriction endonuclease digestion profiles (REDP) of genomic DNA with SfiI. The results revealed a high-level of variation in REDPs, indicating a vast genomic diversity among V. vulnificus strains. Genetic relatedness of the strains showed similarities ranging from 10％ to 100％. Different REDPs for isolates from various raw seafoods were obtained, and clustering of strains according to type of seafoods was not observed. In contrast, clinical isolates of V. vulnificus showed higher similarity to one another, and could be subdivided into one separate group. The difference in REDPs of the V. vulnificus isolates from clinical origin and from raw seafoods substantiates the previous observation that only a single type of pathogenic strain was involved in each human infection, despite the numerous genetically polymorphic strains found from implicated oysters.

• 한국환경과학회지
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• 제5권5호
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• pp.605-610
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• 1996

시판중인 생굴을 대상으로 Vibrio 속을 숙주로 하는 bacteriophage의 분리를 시도하였다. 4 종의 Vibrio 속과 5 혈청형의 Vibrio parahaemolytius를 실험대상으로 한 결과 배brio parahaemolyticus 2 혈청형에서만 bacteriophage가 분리되었다. 분리된 phage의 plaque 크기는 0.4mm였으며 전자현미경적 형태는 미부가 뚜렷하지 않은 육각형의 두부가 관찰되었고 크기는 67nm$\times$83nm 였으며, PFV/ml은 1.25$\times$$10^{11}$이었다. 분리된 phage는 chloro-form에 감수성을 나타내었다. 분리된 phage의 genomic 특성을 규명하기 위하여 핵산을 분리한 결과 DNA로 판명되어졌으며 두 혈청형 모두 제한효소 처리한 결과 Eco R I으로 4부위의 절단양상과 Hind III로부터 14부위 절단양상이 관찰되었다.

• 한국환경과학회지
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• 제25권3호
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• pp.337-343
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• 2016

The present study aimed to determine the influence of various root restriction media on seedling quality and early growth of strawberry after transplanting. The root activity of the seedlings, measured 20 days after fixation, was considerably higher (0.096, 0.090, and $0.063mg{\cdot}g^{-1}{\cdot}h^{-1}$ at 420, 450, and 480 nm, respectively) in expanded rice hull (ERH) treatment than in the sandy loam and loamy sand treatments. The volumetric water content (VWC) of the root media tested across 3 irrigation regimes (15 d, 30 d, 45 d) in the nursery field was highest in sandy loam (65.0-66.8%), followed by 59.4-61.3% in loamy sand and 38.6-45.3% in ERH. When growth parameters of runner plantlets were compared, ERH treatment was found to result in the highest crown thickness and fresh weights of root and above-ground parts. This had a favorable influence on above-ground tissue growth after transplanting to plastic house soil. As mentioned above, ERH treatment resulted in the highest seedling quality and early growth after transplanting. The results of this study would serve as useful on-site data for the production of high-quality strawberry seedlings.

• 청정기술
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• 제14권4호
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• pp.225-231
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• 2008

최근 주요 선진국을 중심으로 환경규제가 전면적으로 확대되어가고 있다. 특히 작년 발효된 REACH(Registration, Evaluation, Authorization and Restriction of Chemicals) 제도의 경우 우리나라의 주요 수출품이 모두 규제 대상 물품이 될 수 있기 때문에 환경규제에 대한 대응이 미흡할 경우 수출중심의 우리나라 경제에 결정적인 타격이 예상 된다. 그러므로 본고에서는 산업경쟁력 유지 및 지속가능성의 확보를 위한 기반을 마련하고자 REACH에 대한 전반적인 이해와 더불어 최근 REACH의 준비 및 현황에 대해 조사하였으며, 현재 기업 및 정부대응의 문제점을 짚어보고 REACH 관련 전문가들의 수렴된 의견을 통해 정책을 제언하고자 하였다.