Journal of Microbiology and Biotechnology
- Volume 9 Issue 1
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- Pages.78-83
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- 1999
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- 1017-7825(pISSN)
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- 1738-8872(eISSN)
Intraspecific Variation of Environmental and Clinical Vibrio vulnificus Isolates as Demonstrated by Restriction Endonuclease Digestion Profiles
- Kim, Ki-Yong (Department of Food Science and Technology, Institute of Biotechnology and Center for Specialized Agricultural Sciences, Chonnam National University) ;
- Yang, Ho-Chul (Department of Food Science and Technology, Institute of Biotechnology and Center for Specialized Agricultural Sciences, Chonnam National University) ;
- Tamplin, Mark-L. (Department of Food Science and Human Nutrition, Department of Microbiology and Cell Science, Institute of Food and Agricultural Science, University of Florida) ;
- Choi, Sang-Ho (Department of Food Science and Technology, Institute of Biotechnology and Center for Specialized Agricultural Sciences, Chonnam National University)
- Published : 1999.02.01
Abstract
Thirty-six environmental isolates of Vibrio vulnificus obtained from seawater, sediments, and raw seafoods, and 18 clinical isolates from Vibrio septicemia patients were typed by restriction endonuclease digestion profiles (REDP) of genomic DNA with SfiI. The results revealed a high-level of variation in REDPs, indicating a vast genomic diversity among V. vulnificus strains. Genetic relatedness of the strains showed similarities ranging from 10％ to 100％. Different REDPs for isolates from various raw seafoods were obtained, and clustering of strains according to type of seafoods was not observed. In contrast, clinical isolates of V. vulnificus showed higher similarity to one another, and could be subdivided into one separate group. The difference in REDPs of the V. vulnificus isolates from clinical origin and from raw seafoods substantiates the previous observation that only a single type of pathogenic strain was involved in each human infection, despite the numerous genetically polymorphic strains found from implicated oysters.