• 한국지하수토양환경학회지:지하수토양환경
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• 제19권5호
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• pp.9-17
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• 2014

This paper reported the use of real-time polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), and the culture-based method in the intrinsic bioremediation study at a petroleum contaminated site. The study showed that phenol hydroxylase gene was detected in groundwater contaminated with benzene, toluene, ethylbenzene, xylene isomers (BTEX) and methyl tert-butyl ether (MTBE). This indicated that intrinsic bioremediation occurred at the site. DGGE analyses revealed that the petroleum-hydrocarbon plume caused the variation in microbial communities. MTBE degraders including Pseudomonas sp. NKNU01, Bacillus sp. NKNU01, Klebsiella sp. NKNU01, Enterobacter sp. NKNU01, and Enterobacter sp. NKNU02 were isolated from the contaminated groundwater using the cultured-based method. Among these five strains, Enterobacter sp. NKNU02 is the most effective stain at degrading MTBE without the addition of pentane. The MTBE biodegradation experiment indicated that the isolated bacteria were affected by propane. Biodegradation of MTBE was decreased but not totally inhibited in the mixtures of BTEX. Enterobacter sp. NKNU02 degraded about 60% of MTBE in the bioreactor study. Tert-butyl alcohol (TBA), acetic acid, 2-propanol, and propenoic acid were detected using gas chromatography/mass spectrometry during MTBE degraded by the rest cells of Enterobacter sp. NKNU02. The effectiveness of bioremediation of MTBE was assessed for potential field-scale application.

• 콘크리트학회논문집
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• 제17권5호
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• pp.857-868
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• 2005

This paper deals with the accumulated damage in concrete structures due to the cyclic freeze-thaw as an environmental load. The cyclic ice body nucleation and growth processes in porous systems are affected by the thermo-physical and mass transport properties, and gradients of temperature and chemical potentials. Furthermore, the diffusivity of deicing chemicals shows significantly higher value under cyclic freeze-thaw conditions. Consequently, the disintegration of concrete structures is aggravated at marine environments, higher altitudes, and northern areas. However, the properties of cyclic freeze-thaw with crack growth and diffusion of chloride ion effects are hard to be identified in tests, and there has been no analytic model for the combined degradations. The main objective is to determine the driving force and evaluate the reduced strength and stiffness by freeze-thaw. For the development of computational model of those coupled deterioration, micro-pore structure characterization, pore pressure based on the thermodynamic equilibrium, time and temperature dependent super-cooling with or without deicing salts, nonlinear-fracture constitutive relation for the evaluation of internal damage, and the effect of entrained air pores (EA) has been modeled numerically. As a result, the amount of ice volume with temperature dependent surface tensions, freezing pressure and resulting deformations, and cycle and temperature dependent pore volume has been calculated and compared with available test results. The developed computational program can be combined with DuCOM, which can calculate the early aged strength, heat of hydration, micro-pore volume, shrinkage, transportation of free water in concrete. Therefore, the developed model can be applied to evaluate those various practical degradation cases as well.

• Nutrition Research and Practice
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• 제7권1호
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• pp.3-8
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• 2013

Buckwheat is known as a health food but is one of the major food allergens triggering potentially fatal anaphylaxis in Asia, especially in Japan and Korea. This study was conducted to investigate the characteristic of enzymatic resistance of buckwheat protein and allergenic potential. Enzymatic resistance of buckwheat protein was performed with in vitro digestibility test in simulated gastric fluid (SGF), pH 1.2, using pepsin and simulated intestinal fluid (SIF) using chymotrypsin. Reactivity of buckwheat proteins to human IgE was performed using six allergic patients sensitized to buckwheat. Buckwheat's IgE levels were measured using the Phadia UniCAP-system. Buckwheat protein, 16 kDa, still remained after 30 min treatment of pepsin on SDS-PAGE. Even though 16 kDa almost disappeared after 60 min treatment, two out of the six buckwheat patients' sera showed reactivity to hydrolysate after 60 min treatment, indicating that allergenicity still remained. In simulated intestinal fluid (SIF) using chymotrypsin, buckwheat protein, 24 kDa, showed resistance to hydrolysis with chymotrypsin on SDS-PAGE, and still had allergenicity based on the result of ELISA. Our results suggest that buckwheat proteins have strong resistance to enzyme degradation. This may be attributed in part to the allergenic potential of buckwheat. Further study should be continued regarding buckwheat allergy.

• 한국산학기술학회논문지
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• 제14권2호
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• pp.911-918
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• 2013

2차원 RMA2 모형을 이용하여 방류조건에 따른 댐하류 하천의 홍수위, 유속분포 및 하상변동을 분석하였다. 수위분석결과 200년빈도에 의한 영향은 주암댐에서 광천교까지 구간(1.27km)에서 나타났고, 여유고는 댐 직하류 좌안제방 300m 구간에서 0.7m 부족하였다. PMF 방류시는 보성강 전구간에서 제방을 월류하는 것으로 분석되었다. 침수범람구역은 200년 빈도에서는 하천합류부의 일부지점에서 발생하였고, PMF 방류시에는 제방월류로 침수구역이 확대되었다. 하도내의 유속은 교량 및 하도의 폭이 좁은 구간에서 비교적 유속이 크게 나타나 하상저하가 발생하였다.

• 한국도서관정보학회지
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• 제42권4호
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• pp.111-136
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• 2011

90년대는 일본 학생들의 문자이탈 현상 및 그에 따른 독해력 저하가 심각한 사회 문제로 대두되었던 독서의 위기 상황이었다. 그러나 이러한 상황을 전환점으로 삼아 학교도서관 및 관련 기관들은 관련 법률을 제정 개정 하고, 학교도서관 및 독서관련 사업을 펼치는 등 다양한 방면에서 어린이 독서활동의 환경 정비에 주력하였다. 본 연구에서는 90년대부터 활발하게 진행된 학교도서관을 둘러싼 독서환경 정비를 위한 각계각층의 노력들을 소개하고 정리함으로써 일본 학교도서관의 거시적 환경을 이해하고 한국의 학교도서관이 나아가야할 방향을 제고한다.

• 한국산학기술학회논문지
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• 제4권3호
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• pp.199-203
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• 2003

In this study plastic optical fibers (POFs) were considered as light-transmitting media and substrates for the potential use in photocatalytic environmental purification system. After the characteristics of POFs in terms of light transmittance and absorption were determined at the beginning, the further investigation was performed through the photocatalytic degradation of trichloroethylene (TCE), iso-propanol and etc. with TiO$_2$-coated optical fiber reactor systems (POFR). It is concluded that the use of POFs is preferred to quartz optical fibers (QOFs) since the advantages such as ease of handling, lower cost, relatively reasonable light attenuation at the wavelength of near 400nm can be obtained. Various geometrical reactor shapes have been constructed and applied for the last one and half years. For the use of POF in water phase treatment, however, more detailed scientific and engineering aspects should be envisaged. This case requires a suitable mixture to obtain more stable and innocuous immobilization of photocatalyst on POF. To overcome this disadvantage, metal-organic chemical vapor deposition (MOCVD) was conducted in a fluidized bed to deposit thin films of titania on glass and alumina beads. Those can be used as photocatalysis for the removal of pollutants especially in liquid phases.

• 대한토목학회논문집
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• 제12권1호
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• pp.19-27
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• 1992

비선형(非線形) 동적하중하(動的荷重下)에서 철근 콘크리트 부재의 이역학동(履歷學動) 응답(應答)을 재생(再生)키 위하여 Roufaiel and Meyer의 이역거동(履歷擧動)모델을 수정 향상시키었다. 본(本) 수정된 모델은 비탄성구간(非彈性區間) 즉, 강복강도(降伏强度)를 넘는 부재에서 발생하는 소성영역(塑性領域)을 고려하였으며 비탄성동적하중하(非彈性動的荷重下)에서의 강성(剛性) 및 강도(强度)저하 현상, 전단력 및 축방향하중의 영향(影響) 등을 모의 모형화하였다. 특히 강도(强度)감소 현상의 모델링 방법은 본(本) 연구(硏究)에서 특기할 사항이다. 제시된 모델의 정확성(正確性)은 많은 실험적인 하중(荷重)-변형(變形)곡선을 수리적(數理的)으로 재생(再生)하여 비교분석(比較分析)함으로써 입증(立證)하였다.

• Toxicological Research
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• 제23권2호
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• pp.123-126
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• 2007

We isolated a lignan, kobusin from Geranium thunbergii and studied its effect on the expression of inducible nitric oxide synthase (iNOS) gene in a monocyte/macrophage cell line, RAW264.7 cells. Kobusin inhibited lipopolysaccharide (LPS)-stimulated NO production and the expression of iNOS in a concentration-dependent manner. To identify the mechanistic basis for its inhibition of iNOS induction, we examined the effect of kobusin on both the luciferase reporter activity using $NF-{\kappa}B$ minimal promoter and the nuclear translocation of p65. Kobusin suppressed the reporter gene activity and the LPS-induced movement of p65 in to nucleus. $NF-{\kappa}B$ activation is controlled by the phosphorylation and subsequent degradation of $I-{\kappa}B{\alpha}$, and in the present study, we found that $I-{\kappa}B{\alpha}$ phosphorylation was also inhibited by kobusin. Our findings indicate that kobusin may provide a developmental basis for an agent against inflammatory diseases.

• 대한기계학회논문집A
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• 제20권2호
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• pp.520-528
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• 1996

Four point bending tests of the brazed joint composed of sintered silicon nitride and 0.2% carbon steel with Cusil ABA filler which were fabricated at 86$0^{\circ}C$ were performed at temperatures, 25, 100, 200, 300, 400, 50$0^{\circ}C$ From the experiments, the maximum bending strength was measured at 30$0^{\circ}C$ From the 3D FE analysis of the residual stress of the brazed joint, it was revealed that the thermally induced residual stresses were minimized when the environmental temperature was 35$0^{\circ}C$ Considering the degradation of the filler material at high temperatures, it was calculated that the maximum bending strength of the brazed joint occured just below the temperature of the minimum thermal residual stress and the thermal residual stress was the dominative parameter of the brazed joint.

• 한국산업융합학회 논문집
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• 제23권3호
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• pp.403-410
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• 2020

Continuous industrial development has led to a better quality of life for everyone, even further accelerating industrial growth. Industrial development, however, has also caused environmental degradation, which is posing a serious threat to humanity. It has also encouraged the indiscriminate use of limited resources, causing resource depletion. Efficient resource management based on resource circulation is critical to saving resources. Resource circulation methods are as follows: reducing the use of resources in the manufacturing process, recycling used or reprocessed products and reusing used resources without being reprocessed, remanufacturing with end-of-life products with disassembled parts. Furthermore, remanufacturing process including cleaning, inspection, repairing, and reassembling facilitate performance level as well as new typical products. It is noteworthy that the remanufacturing of machine tools can significantly save resources because their structural parts are substantially large in size. Machine tools have served as a foundation for the manufacturing industry, which has driven Korea's industrial development. Nevertheless, a few research has been reported for remanufacturing technology with used machine tools. Relevant research of developing a remanufacturing process chart and method is prerequisite for saving the resource and environments.