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http://dx.doi.org/10.4162/nrp.2013.7.1.3

Allergenic potential and enzymatic resistance of buckwheat  

Lee, Sujin (Department of Food Science and Technology, Ewha Womans University)
Han, Youngshin (Environmental Health Center for Atopic Diseases, Samsung Medical Center, Sungkyunkwan University School of Medicine)
Do, Jeong-Ryong (Korea Food Research Institute)
Oh, Sangsuk (Department of Food Science and Technology, Ewha Womans University)
Publication Information
Nutrition Research and Practice / v.7, no.1, 2013 , pp. 3-8 More about this Journal
Abstract
Buckwheat is known as a health food but is one of the major food allergens triggering potentially fatal anaphylaxis in Asia, especially in Japan and Korea. This study was conducted to investigate the characteristic of enzymatic resistance of buckwheat protein and allergenic potential. Enzymatic resistance of buckwheat protein was performed with in vitro digestibility test in simulated gastric fluid (SGF), pH 1.2, using pepsin and simulated intestinal fluid (SIF) using chymotrypsin. Reactivity of buckwheat proteins to human IgE was performed using six allergic patients sensitized to buckwheat. Buckwheat's IgE levels were measured using the Phadia UniCAP-system. Buckwheat protein, 16 kDa, still remained after 30 min treatment of pepsin on SDS-PAGE. Even though 16 kDa almost disappeared after 60 min treatment, two out of the six buckwheat patients' sera showed reactivity to hydrolysate after 60 min treatment, indicating that allergenicity still remained. In simulated intestinal fluid (SIF) using chymotrypsin, buckwheat protein, 24 kDa, showed resistance to hydrolysis with chymotrypsin on SDS-PAGE, and still had allergenicity based on the result of ELISA. Our results suggest that buckwheat proteins have strong resistance to enzyme degradation. This may be attributed in part to the allergenic potential of buckwheat. Further study should be continued regarding buckwheat allergy.
Keywords
Buckwheat; hydrolysis; pepsin; chymotrypsin; allergenicity;
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1 IUIS Allergen Nomenclature [Internet]. Allergens of Buckwheat. [cited 2012 July 14]. Available from: http://www.allergen.org/.
2 Takagi K, Teshima R, Okunuki H, Sawada J. Comparative study of in vitro digestibility of food proteins and effect of preheating on the digestion. Biol Pharm Bull 2003;26:969-73.   DOI   ScienceOn
3 Koyano S, Takagi K, Teshima R, Sawada J. Molecular cloning of cDNA, recombinant protein expression and characterization of a buckwheat 16-kDa major allergen. Int Arch Allergy Immunol 2006;140:73-81.   DOI   ScienceOn
4 Satoh R, Koyano S, Takagi K, Nakamura R, Teshima R, Sawada J. Immunological characterization and mutational analysis of the recombinant protein BWp16, a major allergen in buckwheat. Biol Pharm Bull 2008;31:1079-85.   DOI   ScienceOn
5 The Korean Academy of Asthma. Allergy and Clinical Immunology, 4 Chunmanyoi Allergy. Seoul: Yoihakmunhwasa Publishing Co.; 2005. p.85-92.
6 Smith HL. Buckwheat-poisoning with report of a case in man. Arch Intern Med (Chic) 1909;3:350-9.
7 Son DY, Yoon KR, Lee SI. Study of the most common allergic foods in Korea. Korean J Food Sci Technol 2002;34:885-8.
8 Houben GF, Knippels LM, Penninks AH. Food allergy: predictive testing of food products. Environ Toxicol Pharmacol 1997;4:127-35.   DOI   ScienceOn
9 Tanaka K, Matsumoto K, Akasawa A, Nakajima T, Nagasu T, Iikura Y, Saito H. Pepsin-resistant 16-kD buckwheat protein is associated with immediate hypersensitivity reaction in patients with buckwheat allergy. Int Arch Allergy Immunol 2002;129: 49-56.   DOI   ScienceOn
10 Kruger NJ. The bradford method for protein quantitation. In: Walker JM, editor. The Protein Protocols Handbook. 2nd ed. Totowa: Humana Press Inc.; 2002. p.15-21.
11 Kato H, van Chuyen N, Utsunomiya N, Okitani A. Changes of amino acids composition and relative digestibility of lysozyme in the reaction with alpha-dicarbonyl compounds in aqueous system. J Nutr Sci Vitaminol (Tokyo) 1986;32:55-65.   DOI
12 Keum EH, Lee SI, Oh S. Effect of enzymatic hydrolysis of 7S globulin, a soybean protein, on its allergenicity and identification of its allergenic hydrolyzed fragments using SDS-PAGE. Food Sci Biotechnol 2006;15:128-32.
13 Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970;227:680-5.   DOI   ScienceOn
14 Matsumoto R, Fujino K, Nagata Y, Hashiguchi S, Ito Y, Aihara Y, Takahashi Y, Maeda K, Sugimura K. Molecular characterization of a 10-kDa buckwheat molecule reactive to allergic patients' IgE. Allergy 2004;59:533-8.   DOI   ScienceOn
15 Park JW, Kang DB, Kim CW, Ko SH, Yum HY, Kim KE, Hong CS, Lee KY. Identification and characterization of the major allergens of buckwheat. Allergy 2000;55:1035-41.   DOI   ScienceOn
16 Han Y, Kim J, Ahn K. Food allergy. Korean J Pediatr 2012;55: 153-8.   DOI   ScienceOn
17 Nair A, Adachi T. Immunodetection and characterization of allergenic proteins in common buckwheat (Fagopyrum esculentum). Plant Biotechnol (Tsukuba) 1999;16:219-24.   DOI
18 Kondo Y, Urisu A, Wada E, Tsuruta M, Yasaki T, Yamada K, Masuda S, Morita Y. Allergen analysis of buckwheat by the immunoblotting method. Arerugi 1993;42:142-8.
19 Urisu A, Kondo Y, Morita Y. Identification of a major allergen of buckwheat seeds by immunoblotting methods. Allergy Clin Immunol News 1994;6:151-5.
20 Yoshimasu MA, Zhang JW, Hayakawa S, Mine Y. Electrophoretic and immunochemical characterization of allergenic proteins in buckwheat. Int Arch Allergy Immunol 2000;123:130-6.   DOI   ScienceOn
21 Satoh R, Koyano S, Takagi K, Nakamura R, Teshima R. Identification of an IgE-binding epitope of a major buckwheat allergen, BWp16, by SPOTs assay and mimotope screening. Int Arch Allergy Immunol 2010;153:133-40.   DOI   ScienceOn
22 Wieslander G, Norback D. Buckwheat allergy. Allergy 2001;56: 703-4.   DOI   ScienceOn
23 Park CH, Choi YS. Memil. Chuncheon: Kangwon National University Publishing Co.; 2004. p.35-43.
24 He J, Klag MJ, Whelton PK, Mo JP, Chen JY, Qian MC, Mo PS, He GQ. Oats and buckwheat intakes and cardiovascular disease risk factors in an ethnic minority of China. Am J Clin Nutr 1995;61:366-72.   DOI
25 Choe M, Kim JD, Park KS, Oh SY, Lee SY. Effect of buckwheat supplementation on blood glucose levels and blood pressure in rats. J Korean Soc Food Nutr 1991;20:300-5.