Browse > Article
http://dx.doi.org/10.7857/JSGE.2014.19.5.009

Evaluation of Intrinsic Bioremediation of Methyl Tert-butyl Ether (MTBE) Contaminated Groundwater  

Chen, Colin S. (Department of Biotechnology, National Kaohsiung Normal University)
Tien, Chien-Jun (Department of Biotechnology, National Kaohsiung Normal University)
Zhan, Kai-Van (Sinotech Environmental Technology Co, Ltd.)
Publication Information
Journal of Soil and Groundwater Environment / v.19, no.5, 2014 , pp. 9-17 More about this Journal
Abstract
This paper reported the use of real-time polymerase chain reaction (PCR), denaturing gradient gel electrophoresis (DGGE), and the culture-based method in the intrinsic bioremediation study at a petroleum contaminated site. The study showed that phenol hydroxylase gene was detected in groundwater contaminated with benzene, toluene, ethylbenzene, xylene isomers (BTEX) and methyl tert-butyl ether (MTBE). This indicated that intrinsic bioremediation occurred at the site. DGGE analyses revealed that the petroleum-hydrocarbon plume caused the variation in microbial communities. MTBE degraders including Pseudomonas sp. NKNU01, Bacillus sp. NKNU01, Klebsiella sp. NKNU01, Enterobacter sp. NKNU01, and Enterobacter sp. NKNU02 were isolated from the contaminated groundwater using the cultured-based method. Among these five strains, Enterobacter sp. NKNU02 is the most effective stain at degrading MTBE without the addition of pentane. The MTBE biodegradation experiment indicated that the isolated bacteria were affected by propane. Biodegradation of MTBE was decreased but not totally inhibited in the mixtures of BTEX. Enterobacter sp. NKNU02 degraded about 60% of MTBE in the bioreactor study. Tert-butyl alcohol (TBA), acetic acid, 2-propanol, and propenoic acid were detected using gas chromatography/mass spectrometry during MTBE degraded by the rest cells of Enterobacter sp. NKNU02. The effectiveness of bioremediation of MTBE was assessed for potential field-scale application.
Keywords
Real-time polymerase chain reaction (PCR); Denaturing gradient gel electrophoresis (DGGE); Methyl tertbutyl ether (MTBE) degradation; Enterobacter sp. NKNU02;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Deeb, R.A., Chu, K.H., Shih T., Linder, S., Suffet, I.H., Kavanaugh, M.C., and Alvarez-Cohen, L., 2003, MTBE and other oxygenates: environmental sources, analysis, occurrence and treatment, Environ. Eng. Sci., 20, 433-447.   DOI   ScienceOn
2 Baldwin, B.R., Nakatsu, C.H., and Nies, L., 2008, Enumeration of aromatic oxygenase genes to evaluate monitored natural attenuation at gasoline-contaminated sites, Water Res., 42, 723-731.   DOI   ScienceOn
3 Balwin, B.R., Nakatsu, C.H., Nebe, J., and Wickham, G.S., 2009, Enumeration of aromatic oxygenase genes to evaluate biodegradation during multi-phase extraction at a gasoline-contaminated site, J. Hazard. Mater., 163, 524-530.   DOI   ScienceOn
4 Chen, S.C., Chen, C.S., Zhan, K.V., Yang, K.H., Chien, C.C., Shieh, B.S., and Chen, W.M., 2011, Biodegradation of methyl tert-butyl ether (MTBE) by Enterobacter sp. NKNU02, J. Hazard. Mater., 186, 1744-1750.   DOI   ScienceOn
5 Eixarch, H. and Constani, M., 2010, Biodegradtion of MTBE by Achromobacter xylosoxidans MCM1/1 induces synthesis of proteins that may be related to cell survival, Process Biochem., 45, 794-798.   DOI   ScienceOn
6 Ferreira, N.L., Malanndain, C., and Guichard, F.F., 2006, Enzymes and genes involved in the aerobic biodegradation of methyl tert-butyl ether (MTBE), Appl. Microbiol. Biotechnol., 72, 252-262.   DOI   ScienceOn
7 Fischer, A., Muller, M., and Klasmeier, J., 2004, Determination of Henry's law constant for methyl tert-butyl ether (MTBE) at groundwater temperatures, Chemosphere, 54, 689-694.   DOI   ScienceOn
8 Lee, E.H. and Cho, K.S., 2009, Effect of substrate interaction on the degradation of methyl tert-butyl ether, benzene, toluene, ethylbenzene, and xylene by Rhodococcus sp., J. Hazard. Mater. 167, 669-674.   DOI   ScienceOn
9 Francois, A., Garnier, L. Mathis, H., Fayolle, F., and Monot, F., 2003, Roles of tert-butyl formate, tert-butyl alchol and acetone in the regulation of methyl tert-butyl ether degradation by Mycobacterium austroafricanium IFP 2012, Appl. Microbiol. Biotechnol., 62, 256-262.   DOI
10 Kao, C.M., Chen, C.S., Tsa, F.Y, Yang, K.H., Chien, C.C., Liang, S.H., Yang, C.A., and Chen, S.C., 2010, Application of real-time PCR, DGGE fingerprinting, and culture-based method to evaluate the effectiveness of intrinsic bioremediation on the control of petroleum-hydrocarbon plume, J. Hazard. Mater., 178, 409-416.   DOI   ScienceOn
11 Labbe, D., Margesin, R., Schinner, F., Whyte, L., and Greer, C.W., 2007, Comparative phylogenetic analysis of microbial communities in pristine and hydrocarbon-contaminated Alpine soils, FEMS Microbiol. Ecol., 59, 466-475.   DOI   ScienceOn
12 Lin, B., Van Verseveld, H.W., and Rolling, W.F., 2002, Microbial aspects of anaerobic BTEX degradation, Biomed. Environ. Sci., 15, 130-144.
13 Nebr, I., Baldwin, B.R., Kassab, R., Nies, L., and Nakatsu, C.H. 2009, Quantification of aromatic oxygenase genes to evaluate enhanced bioremediation by oxygen releasing materials at a gasoline-contaminates site, Environ. Sci. Technol., 43, 2029-2034.   DOI   ScienceOn
14 Okeke Jr., B.C. and Frankenberger, W.T., 2003, Biodegradation of methyl tertiary butyl ether (MTBE) by a bacterial enrichment consortia and its monoculture isolates, Microbiol. Res., 158, 99-106.   DOI   ScienceOn
15 Pruden, A., Suidan, M.T., Venosa, A.D., and Wilson, G.J., 2001, Biodegradation of methyl tert-butyl ether under various substrate conditions, Environ. Sci. Technol., 35, 4235-4241.   DOI   ScienceOn
16 Salanitro, J.P., Diaz, L.A., Williams, M.P., and Wisniewski, H.L., 1994, Isolation of a bacterial culture that degrades methylt-butyl ether. Appl. Environ. Microbiol., 60, 2593-2596.
17 Schmidt, T.C., Schirmer, M., Weib, H., and Haderlein, S., 2004, Microbial degradation of methyl tert-butyl ether and tert-butyl alcohol in the subsurface, J. Contam. Hydrol., 70, 173-203.   DOI   ScienceOn
18 Pruden, A. and Suidan, M., 2004, Effect of benzene, toluene, ethylbenzene, and p-xylene (BTEX) mixture on biodegradation of methyl tert-butyl ether (MTBE) and tert-butyl alchol (TBA) by pure culture UC1, Biodegradation, 15, 213-227.   DOI   ScienceOn
19 Rooney-Varga, J.N., Anderson, R.T., Fraga, J.L., Ringelberg, D., and Lovley, D.R., 1999, Microbial communities associated with anaerobic benzene degradation in a petroleum-contaminated aquifer, Appl. Environ. Microbiol., 65, 3056-3063.
20 Saitou, N. and Nei, M., 1987, The neighbour-joining method: a new method for reconstructing phylogenetic tress. Mol. Biol. Evol., 4, 406-425.
21 Smith, C.A., O'Reilly, K.T., and Hyman, M.R., 2003, Characterization of the initial reactions during the cometabolic oxidation of methyl tert-butyl ether by propane-grown Mycobacterium vaccae IOB5, Appl. Microbiol. Biotechnol., 69, 796-804.
22 Smith, C.A. and Hyman, M.R., 2004, Oxidation of methyl tertbutyl ether by alkane hydroxylase in dicyclopropylketone-induced and n-octane-grown Pseudomonas putida Gpo1, Appl. Environ. Microbiol., 70, 4544-4555.   DOI   ScienceOn
23 Sulfita, J. M. and Mormile, M.R., 1993, Anaerobic biodegradation of known and potential gasoline oxygenates in the terrestrial subsurface. Environ. Sci. Technol., 27, 976-978.   DOI   ScienceOn
24 TEPA (Taiwan Environmental Protection Administration) 1999, Toxic Chemical Substances Management Act, http://law.epa.gov.tw/en/laws/toxics/.
25 Chen, C.S., Hseu, Y.C., Liang, S.H., Kuo, J.Y., and Chen, S.C., 2008, Assessment of genotoxicity of methyl-tert-butyl ether, benzene, toluene, ethylbenzene, and xylene to human lymphocytes using comet assay, J. Hazard Mater., 53, 351-356.
26 Zhang, L.L. Zhu, R.Y., Chen, J.M., and Cai, W.M., 2008, Biodegradation of methyl tert-butyl ether as a sole carbon by aerobic granules cultivated in a sequencing batch reactor, Bioprocess Biosyst. Eng., 31, 527-534.   DOI   ScienceOn
27 US EPA (US Environmental Protection Agency) 1997, Drinking water advisory: Consumer acceptability advice and health effects analysis on methyl tertiary-butyl ether (MTBE), EPA 822-F-97-009.
28 Volpe, A., Moro, G.D., Rossetti, S., Tandoi, V., and Lopez, A., 2009, Enhanced bioremediation of methyl tert-butyl ether (MTBE) by microbial consortia obtained from contaminated aquifer material, Chemosphere, 75, 149-155.   DOI   ScienceOn