• Journal of Environmental Science International
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• v.25 no.6
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• pp.789-798
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• 2016

Lactic acid bacteria (LAB) are industrially important microorganisms for probiotics. The recent widespread application of LAB for preparation of functional food is attributable to the accumulating scientific evidence showing their beneficial effects on human health. In this study, we isolated and characterized plant-derived LAB that show angiotensin-converting enzyme (ACE) inhibitory and antioxidant activities. The selected strain K2 was isolated from Kimchi, and identified as Lactobacillus plantarum by 16S rRNA gene analysis. The strain grew under static and shaking culture systems. They were also able to grow in different culture conditions like $25^{\circ}C{\sim}37^{\circ}C$ temperature, 4~10 pH range and ~6% NaCl concentration. L. plantarum K2 was highly resistant to acid stress; survival rate of the strain at pH 2.5 and 3 were 80% and 91.6%, respectively. The strain K2 also showed high bile resistance to 0.3% bile bovine and 0.3% bile extract with more than 74% of survival rate. The cell grown on MRS agar plate containing bile extract formed opaque precipitate zones around the colonies, indicating they have bile salt hydrolase activity. The strain showed an inhibitory activity against pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Listeria monocytogenes; antibacterial activity was probably due to the lactic acid. The K2 strain showed relatively higher autoaggregation values, antihypertensive and antioxidant activities. These results suggest that L. plantarum K2 could be not only applied as a pharmabiotic for human health but also is also starter culture applicable to fermentative products.

• Journal of Industrial Convergence
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• v.20 no.6
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• pp.1-6
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• 2022

This study started with the design methodology for externally installed metal sculptures. As the scope and meaning of design grows, the concept of design is also used for externally installed sculptures, and there is a demand for a sculpture design that contains the story of the installation environment while being suitable for the surrounding environment. This concept is defined as an environmental sculpture only among formative artists. The concept of environmental sculptures is not fully known and academic research is also insufficiently performed. Therefore, in order to define externally installed sculptures as environmental sculptures, this study elaborated the concept and conditions of good design and the concept and role of environmental sculptures as a theoretical background, and classified the concepts and conditions of good design and the concepts and roles of environmental sculptures. Based on the previous theoretical studies, by utilizing the conditions of good design and the role of environmental sculptures, this study suggested that design development elements were completed as metal environmental sculptures and research works were presented. In conclusion, the design methodology for metal environmental sculptures has been presented, and various environmental sculpture design studies will continue and various paradigms of convergence environmental sculptures will be studied.

• Journal of Environmental Science International
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• v.27 no.9
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• pp.771-780
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• 2018

To develope a microbial weed control agent, HCN-producing bacteria were isolated, and their characteristics were investigated. A selected strain of WA15 was identified as Pseudomonas koreensis by morphological, cultural, biochemical and 16S rRNA gene analyses. The conditions for HCN production was investigated by a One-Variable-at-a-Time (OVT) method. The optimal HCN production conditions were tryptone 1%, glycine 0.06%, NaCl 1%, and an initial pH and temperature of 5.0 and $30^{\circ}C$, respectively. The major component for HCN production was glycine. Under optimal conditions, HCN production was about 3 times higher than that of the basal medium. The WA15 strain had physiological activities, such as indoleacetic acid that was associated with the elongation of plant roots and siderophore and ammonification inhibiting fungal growth, and produced hydrolytic enzymes, such as cellulase, pectinase and lipase. The strain was able to inhibit the growth of phytopathogenic fungi, such as Rhizoctonia solani, Botrytis cinerea and Fusarium oxysporum, by the synergistic action of volatile HCN and diffusible antimicrobial compounds. A microscopic observation of R. solani that was teated with the WA15 strain showed morphological abnormalities of fungal mycelia, which could explain the role of the antimicrobial metabolites that were produced by the WA15 strain. The volatile HCN produced by the WA15 strain was also found to have insecticidal activity against termites. Our results indicate that Pseudomonas koreensis WA15 can be applied as a microbial agent for weed control and also as a termite repellent. Furthermore, it could be applied as a microbial termiticidal agent to replace synthetic insecticides.

• Journal of Environmental Science International
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• v.29 no.8
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• pp.819-826
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• 2020

Production of Bacterial Cellulose (BC) by Gluconacetobacter sp. A5 was studied in shaken culture using different cost-effective carbon sources and its structural and mechanical properties were evaluated. Glycerol showed the highest level (7.26 g/l) of BC production, which was about three times higher than the yield in glucose medium. BC production depended not only on the decrease in pH, but also on the ability of Gluconacetobacter sp. A5 to synthesize glucose from different carbon sources and then polymerize it into BC. All BC produced from different carbon sources exhibited a three-dimensional reticulated structure consisting of ultrafine cellulose fibriles. Carbon sources did not significantly change the microfibrile structure of the resulting BC. BC produced from glucose medium had the lowest water-holding capacity, while BC from molasses medium had the highest. XRD data revealed that all BC were cellulose type I, the same as typical native cellulose. The crystalline strength of BC produced in glucose medium was the highest, and that in molasses medium was the lowest. Our results suggest that glycerol could be a potential low-cost substrate for BC production, leading to the reduction in the production cost, and also to produce BC with different mechanical properties by selecting appropriate carbon source.

• Journal of Environmental Science International
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• v.31 no.3
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• pp.265-274
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• 2022

Lipoxygenase is an enzyme, mainly produced by plants, capable of converting unsaturated fatty acids to fatty acids. It has vast application potential in the food, pharmaceutical and agricultural industries. The aim of this study was to isolate novel lipoxygenase-producing bacteria from the environment and to investigate the lipoxygenase enzymatic properties for industrial production. The strain, NC1, isolated from cultivation soils, was identified as Bacillus subtilis based on the phenotypic characteristics and 16S rRNA gene sequencing. This strain formed a pink color around the colony when cultured on indamine dye formation plates. The production of lipoxygenase by B. subtilis NC1 was influenced by the composition of the medium and linoleic acid concentrations. The optimum temperature and pH for lipoxygenase activity was determined to be 40 ℃ and pH 6, respectively. The enzyme showed relatively high stability at temperatures ranging from 20-50 ℃ and acid-neutral regions. In addition, the lipoxygenase produced by B. subtilis NC1 was able to degrade commercially available oils including sunflower seed oil and Perilla oil. In this study, a useful indigenous bacterium was isolated, and the fundamental physicochemical data of bacterial lipoxygenase giving it industrial potential are presented.

• Journal of Digital Convergence
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• v.13 no.8
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• pp.123-131
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• 2015

Modern is the era of convergence. In the complex position of the company to survive in the rapid environmental changes it can not be a mandatory fusion rather than choice. Wind convergence also in the art can be said to castration. This phenomenon can be found in many real life. Most real-life contact with the service sector is no exception. Due to a number of banks and insurance combined Banca named fusion of French banking and insurance business are integrated. Even recently, the situation has been provided by Investment consulting, equity trading services within bank branches. In this study, based on the need for convergence of corporate businesses convergence factors examined through regression analysis on whether any impact on the financial aptitude. External environmental factors that make up the fusion research factors internal environmental factors, both core competencies were factors to affect financial performance.

• Journal of the Korean Society of Manufacturing Process Engineers
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• v.21 no.8
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• pp.9-18
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• 2022

As the COVID-19 continues, the demand for robotic technology that can be applied in face-to-face tasks such as delivery and transportation, is increasing. Although these technologies have been developed and applied in various industries, the robots can only be operated in a tidy indoor environment and have limitations in terms of payload. To overcome these problems, we developed a 2 degree of freedom(DOF) environmental adaptive robot leg with a double 1-DOF counterbalance mechanism (CBM) based on wire roller. The double 1-DOF CBM is applied to the two revolute joints of the proposed robot leg to compensate for the weight of the mobile robot platform and part of the payload. In addition, the link of the robot leg is designed in a parallelogram structure based on a belt pulley to enable efficient control of the mobile platform. In this study, we propose the principle and structure of the CBM that is suitable for the robot leg, and design of the counterbalance robot leg module for the environment-adaptive control. Further, we verify the performance of the proposed counterbalance robot leg by using dynamic simulations and experiments.

• Journal of Korean Society of Environmental Engineers
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• v.35 no.8
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• pp.533-539
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• 2013

Korea is one of the top countries that has funded great amount to promote basic research and others in recent decade. While the quantity of R&D fund in Korea has rapidly increased, the effectiveness and quality of R&D outcomes became a controversial issue. National Research Foundation of Korea (NRF) and Korea Environmental Industry & Technology Institute (KEITI) agreed to collaborate for diffusing and utilizing R&D outcomes of each institute. NSF and KEITI signed the bilateral MOU in 2012, and Interministerial Collaborative R&D Support Framework (ICR&DSF) was developed. The ICR&DSF consist of launching an Eco-Bridge Program as an interministerial R&D program and composition of Environmental Convergence R&D Bridge Committee. The Eco-Bridge Program was applied to 'Environmental Convergence Technology Project' of KEITI as a pilot program in 2012. The unique feature of this interministerial R&D program is that the Eco-Bridge Program directly supports the basic research outcomes which are previously funded by NRF. As the collaboration between NRF and KEITI becomes stronger, it is expected to explore the more creative and the more transformative research.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 1993.04a
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• pp.6-6
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• 1993

• Journal of the Korean Society of Environmental Restoration Technology
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• v.24 no.6
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• pp.35-48
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• 2021

In Suwon, the overall hygiene of the city is threatened by the emergence of the rook(Corvus fugilegus) in the city. Rooks began to appear in November of 2016 and has continued to appear from November to March every year. In order to eradicate or to prepare an alternative habitat for rooks, this study aimed to identify the preferred habitat and specific environmental variables. Therefore, in this work, we aim to understand the predicted distribution of rooks in Suwon City with citizen science and through MaxENT, the most widely utilized habitat modeling using citizen science to analyze the preferred habitat of harmful tides appearing in urban areas. In this study, seven environmental variables were chosen: biotope group complex, building floor, vegetation, euclidean distance from farmland, euclidean distance from streetlamp, and euclidean distance from pole and DEM. Among the estimated models, after the time period of sunrise (08:00~18:00) the contribution percentage were as following: euclidean distance from arable land(39.2%), DEM(25.5%), euclidean distance from streetlamp(22.3%), euclidean distance from pole(7.1%), biotope group complex(4.9%), building floor(1%), vegetation(0%). In the time period after sunset(18:00~08:00) the contribution percentage were as following: biotope group complex(437.4%), euclidean distance from pole(26.8%), DEM(13.4%), euclidean distance from streetlamp(11.8%), euclidean distance from farmland(7.9%), building floor(1.4%), vegetation(1.3%).