• 한국환경과학회지
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• 제25권6호
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• pp.789-798
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• 2016

Lactic acid bacteria (LAB) are industrially important microorganisms for probiotics. The recent widespread application of LAB for preparation of functional food is attributable to the accumulating scientific evidence showing their beneficial effects on human health. In this study, we isolated and characterized plant-derived LAB that show angiotensin-converting enzyme (ACE) inhibitory and antioxidant activities. The selected strain K2 was isolated from Kimchi, and identified as Lactobacillus plantarum by 16S rRNA gene analysis. The strain grew under static and shaking culture systems. They were also able to grow in different culture conditions like $25^{\circ}C{\sim}37^{\circ}C$ temperature, 4~10 pH range and ~6% NaCl concentration. L. plantarum K2 was highly resistant to acid stress; survival rate of the strain at pH 2.5 and 3 were 80% and 91.6%, respectively. The strain K2 also showed high bile resistance to 0.3% bile bovine and 0.3% bile extract with more than 74% of survival rate. The cell grown on MRS agar plate containing bile extract formed opaque precipitate zones around the colonies, indicating they have bile salt hydrolase activity. The strain showed an inhibitory activity against pathogenic bacteria such as Escherichia coli, Staphylococcus aureus and Listeria monocytogenes; antibacterial activity was probably due to the lactic acid. The K2 strain showed relatively higher autoaggregation values, antihypertensive and antioxidant activities. These results suggest that L. plantarum K2 could be not only applied as a pharmabiotic for human health but also is also starter culture applicable to fermentative products.

• 산업융합연구
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• 제20권6호
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• pp.1-6
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• 2022

본 논문은 외부 설치 금속조형물의 디자인 방법론에 대한 연구로부터 시작되었다. 디자인의 범위와 의미가 커지면서 외부 설치 조형물에도 디자인의 개념이 사용되고, 조형물에 주변환경에 어울리면서 설치환경의 이야기를 담고 있는 조형물 디자인이 요구되고 있는 실정이다. 이러한 개념은 전문가들 사이에서만 환경조형물이라고 정의되고 있다. 환경조형물의 개념은 아직은 대중적이지 않고 학문적 연구 또한 미흡한 상태이다. 이에 연구자는 외부 설치 조형물을 환경조형물로 정의하기 위해 굿디자인의 개념과 조건과 환경조형물의 개념과 역할을 이론적 배경으로 알아보고 굿디자인의 개념과 조건, 환경조형물의 개념과 역할에 대해 분류하였다. 이론적 배경을 바탕으로 굿디자인의 조건과 환경조형물의 역할을 활용하여 연구자의 디자인 전개 요소를 통해 금속환경조형물로 완성하고 연구작품을 제시하였다. 결과적으로 금속환경조형물의 디자인 방법론을 제시하게 되었고 다양한 환경조형물 디자인 연구가 지속되어 융합적 환경조형물의 다양한 패러다임이 연구되기를 바란다.

• 한국환경과학회지
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• 제27권9호
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• pp.771-780
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• 2018

To develope a microbial weed control agent, HCN-producing bacteria were isolated, and their characteristics were investigated. A selected strain of WA15 was identified as Pseudomonas koreensis by morphological, cultural, biochemical and 16S rRNA gene analyses. The conditions for HCN production was investigated by a One-Variable-at-a-Time (OVT) method. The optimal HCN production conditions were tryptone 1%, glycine 0.06%, NaCl 1%, and an initial pH and temperature of 5.0 and $30^{\circ}C$, respectively. The major component for HCN production was glycine. Under optimal conditions, HCN production was about 3 times higher than that of the basal medium. The WA15 strain had physiological activities, such as indoleacetic acid that was associated with the elongation of plant roots and siderophore and ammonification inhibiting fungal growth, and produced hydrolytic enzymes, such as cellulase, pectinase and lipase. The strain was able to inhibit the growth of phytopathogenic fungi, such as Rhizoctonia solani, Botrytis cinerea and Fusarium oxysporum, by the synergistic action of volatile HCN and diffusible antimicrobial compounds. A microscopic observation of R. solani that was teated with the WA15 strain showed morphological abnormalities of fungal mycelia, which could explain the role of the antimicrobial metabolites that were produced by the WA15 strain. The volatile HCN produced by the WA15 strain was also found to have insecticidal activity against termites. Our results indicate that Pseudomonas koreensis WA15 can be applied as a microbial agent for weed control and also as a termite repellent. Furthermore, it could be applied as a microbial termiticidal agent to replace synthetic insecticides.

• 한국환경과학회지
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• 제29권8호
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• pp.819-826
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• 2020

Production of Bacterial Cellulose (BC) by Gluconacetobacter sp. A5 was studied in shaken culture using different cost-effective carbon sources and its structural and mechanical properties were evaluated. Glycerol showed the highest level (7.26 g/l) of BC production, which was about three times higher than the yield in glucose medium. BC production depended not only on the decrease in pH, but also on the ability of Gluconacetobacter sp. A5 to synthesize glucose from different carbon sources and then polymerize it into BC. All BC produced from different carbon sources exhibited a three-dimensional reticulated structure consisting of ultrafine cellulose fibriles. Carbon sources did not significantly change the microfibrile structure of the resulting BC. BC produced from glucose medium had the lowest water-holding capacity, while BC from molasses medium had the highest. XRD data revealed that all BC were cellulose type I, the same as typical native cellulose. The crystalline strength of BC produced in glucose medium was the highest, and that in molasses medium was the lowest. Our results suggest that glycerol could be a potential low-cost substrate for BC production, leading to the reduction in the production cost, and also to produce BC with different mechanical properties by selecting appropriate carbon source.

• 한국환경과학회지
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• 제31권3호
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• pp.265-274
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• 2022

Lipoxygenase is an enzyme, mainly produced by plants, capable of converting unsaturated fatty acids to fatty acids. It has vast application potential in the food, pharmaceutical and agricultural industries. The aim of this study was to isolate novel lipoxygenase-producing bacteria from the environment and to investigate the lipoxygenase enzymatic properties for industrial production. The strain, NC1, isolated from cultivation soils, was identified as Bacillus subtilis based on the phenotypic characteristics and 16S rRNA gene sequencing. This strain formed a pink color around the colony when cultured on indamine dye formation plates. The production of lipoxygenase by B. subtilis NC1 was influenced by the composition of the medium and linoleic acid concentrations. The optimum temperature and pH for lipoxygenase activity was determined to be 40 ℃ and pH 6, respectively. The enzyme showed relatively high stability at temperatures ranging from 20-50 ℃ and acid-neutral regions. In addition, the lipoxygenase produced by B. subtilis NC1 was able to degrade commercially available oils including sunflower seed oil and Perilla oil. In this study, a useful indigenous bacterium was isolated, and the fundamental physicochemical data of bacterial lipoxygenase giving it industrial potential are presented.

• 디지털융복합연구
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• 제13권8호
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• pp.123-131
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• 2015

현대는 융합의 시대이다. 복잡하고 급격한 환경변화에서 살아남아야 하는 기업의 입장에서는 융합이라는 테마는 선택이 아닌 필수가 아닐 수 없다. 기술 분야에 있어서도 융합의 바람은 거세다고 할 수 있다. 이러한 현상들은 실생활에서도 많이 찾아 볼 수 있다. 실생활에서 가장 많이 접하고 있는 서비스 분야도 예외는 아니다. 융합으로 인하여 은행과 보험이 결합된 방카수랑스란 이름으로 은행업과 보험업이 통합되고 있다. 심지어 최근에는 은행 지점내에서 투자 상담, 주식매매 서비스까지 제공되고 있는 실정이다. 따라서 본 연구에서는 기업의 융합 필요성에 근거를 두고 기업의 융합요인이 재무적 성과에 어떠한 영향을 미치는 지를 회귀분석을 통하여 살펴보았다. 연구 결과 융합요인을 구성하는 외부환경요인, 내부환경요인, 핵심역량요인 모두 재무적 성과에 영향을 미치는 것으로 나타났다.

• 한국기계가공학회지
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• 제21권8호
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• pp.9-18
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• 2022

As the COVID-19 continues, the demand for robotic technology that can be applied in face-to-face tasks such as delivery and transportation, is increasing. Although these technologies have been developed and applied in various industries, the robots can only be operated in a tidy indoor environment and have limitations in terms of payload. To overcome these problems, we developed a 2 degree of freedom(DOF) environmental adaptive robot leg with a double 1-DOF counterbalance mechanism (CBM) based on wire roller. The double 1-DOF CBM is applied to the two revolute joints of the proposed robot leg to compensate for the weight of the mobile robot platform and part of the payload. In addition, the link of the robot leg is designed in a parallelogram structure based on a belt pulley to enable efficient control of the mobile platform. In this study, we propose the principle and structure of the CBM that is suitable for the robot leg, and design of the counterbalance robot leg module for the environment-adaptive control. Further, we verify the performance of the proposed counterbalance robot leg by using dynamic simulations and experiments.

• 대한환경공학회지
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• 제35권8호
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• pp.533-539
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• 2013

국가연구개발 투자가 선진국 수준으로 증가함에 따라 투자 대비 성과 제고를 위해, 부처별 역할과 기능에 따라 상이한 소속부처(연구개발지원기관)간 협업의 필요성이 점차 부각되고 있다. 올해 출범한 신정부에서도 부처간 칸막이를 제거하고, 협업을 통한 국가연구개발사업 투자의 효율성 극대화를 정책목표로 제시하였다. 본 연구에서는 한국연구재단과 한국환경산업기술원 간 우수한 연구성과의 선순환적 활용을 위한 협력사업 추진과 Eco-Bridge 구축 방안을 제시하였다. 양 기관은 2012년 환경융합분야 관련 연구개발사업의 성과제고를 골자로 하는 양해각서(MOU)를 체결하였다. 이후 미래창조과학부와 환경부 간 기초연구성과의 실용화를 위해 한국연구재단 지원으로 기 수행된 기초연구과제를 대상으로 한국환경산업기술원에서 응용연구과제를 공모하는 방식의 협력체제를 국내 최초로 구축하고 환경부 공모 '2013년 환경융합신기술개발사업'으로 향후 3년 동안 약 20억원을 지원하게 된다. 또한 부처간 상설 협의기구인 '환경융합 Bridge 협의회' 설립 및 기초 R&D분야 연구자와 산업체 수요자를 상호 연계하는 Matching Program을 추진할 계획이다. 이와 같은 양 기관 협력체제는 기초 R&D가 시장 예측 및 수요를 기반으로 하는 R&BD의 형태로 발전하는 새로운 모델을 제시하고 나아가 부처별 연구지원 특성을 반영한 범부처 협력체계의 구축으로 국가연구개발사업의 효율성을 제고할 것으로 기대된다.

• 한국환경과학회:학술대회논문집
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• 한국환경과학회 1993년도 봄 학술발표회 초록집
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• pp.6-6
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• 1993

• 한국환경복원기술학회지
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• 제24권6호
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• pp.35-48
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• 2021

In Suwon, the overall hygiene of the city is threatened by the emergence of the rook(Corvus fugilegus) in the city. Rooks began to appear in November of 2016 and has continued to appear from November to March every year. In order to eradicate or to prepare an alternative habitat for rooks, this study aimed to identify the preferred habitat and specific environmental variables. Therefore, in this work, we aim to understand the predicted distribution of rooks in Suwon City with citizen science and through MaxENT, the most widely utilized habitat modeling using citizen science to analyze the preferred habitat of harmful tides appearing in urban areas. In this study, seven environmental variables were chosen: biotope group complex, building floor, vegetation, euclidean distance from farmland, euclidean distance from streetlamp, and euclidean distance from pole and DEM. Among the estimated models, after the time period of sunrise (08:00~18:00) the contribution percentage were as following: euclidean distance from arable land(39.2%), DEM(25.5%), euclidean distance from streetlamp(22.3%), euclidean distance from pole(7.1%), biotope group complex(4.9%), building floor(1%), vegetation(0%). In the time period after sunset(18:00~08:00) the contribution percentage were as following: biotope group complex(437.4%), euclidean distance from pole(26.8%), DEM(13.4%), euclidean distance from streetlamp(11.8%), euclidean distance from farmland(7.9%), building floor(1.4%), vegetation(1.3%).