• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2021.11a
• /
• pp.234-235
• /
• 2021

We set the representative balcony types of the existed building to two types: unexpanded balcony and extended balcony, and analyze the effect of reducing the cooling and heating energy load when applying remodeling. The scope of the study was limited to balcony walls, including window-wall junctions, and was conducted by comparing cases with and without thermal break insulation structures for a clear conclusion. The study was conducted using the equivalent U-value in each case. The equivalent U-value was calculated by deriving through 2 dimensional steady-state heat transfer analysis of each case balcony envelope. And building energy was calculated using the derived equivalent U-value. According to the calculation results, for unexpanded balconies, the equivalent U-value was reduced by about 80%, and the heating and cooling load was reduced by about 20%. In the case of extended balconies, the equivalent U-value was reduced by about 57% and the resulting heating and cooling load was reduced by about 12%.

• Journal of agricultural medicine and community health
• /
• v.21 no.1
• /
• pp.97-105
• /
• 1996

This study conducted to determine the evaluation and improvement for enforcement of the waste recycling system in Pohang city and surveyed 4 areas at the southern and northern districts respectively by a team of two officials during the period 6 February 7 February 1995. The rates of the use for waste recycling system's envelope from home trash were 94.2% at the northern district and 92.8% at the southern district by areas, 95.6% at the group houses (apartments and tenement houses) and 91.3% at the separate houses by patterns of house, the low economic class 96.4%, the middle economic class 96.3% and the upper economic class 87.5% by the economic levels, and the bell method 96.5%, the container method 95.6%, door take method 86.1% in order, respectively. The trash from houses were garbage 57.4%, paper 18.7%, plastic 10.3%, fiber 7.9%, bottle 2.7%, metal and can 1.4%, and wood and rubber 0.7% in order. The rates of the reuse trash(reuse possible paper, plastic, bottle, can and metal) were 20.2% of the total, 21.8% at the southern district and 18.5% at the northern district by areas, 25.7% at the separate houses and 14.7% at the group houses(apartments and tenement houses) by patterns of house, and the door take method 34.3%, the bell take method 17.1% and the container take method 14.7% in order, respectively. There were the double envelopes of the plastic bag from stories 46.3% and the clear plastic bag 29.5% in the waste cycling system's envelopes. There were 1.9 double envelopes in the waste cycling system's envelopes. Garbage occupied more than half of the total trash, so it is need to be compost and provender.

• Korean Journal of Microbiology
• /
• v.21 no.2
• /
• pp.61-70
• /
• 1983

Synchronized cat kidney cells chronically infected with feline leukemia virus (FeLV) were used to study virus production, the synthesis of group specific antigen (gag) and envelope (env) proteins, the expression of env protein on the cell surface during the cell cycle, and the stability of viral RNA. As detecting method, we developed the radioimmunoassay (RIA) system using beta-emission of $^{131}I$ and demonstrated the validity of this system by comparison with routine RIA system using gamma-emission of $^{125}I$. The produced virus was analysed by developed RIA interval was determined by measuring reverse transcriptase activity. The results show that infected cells produce the complete virus particle containing products of gag, env and pol genes of FeLV, and maximum virus production occurs during mitosis of synchronized cells. Labeling of the cell surface of synchronized cells with $^{131}I$ shows that the amount of $gp70^{env}$ on the cell surface parallels cellular gorwth. Therefore, the cell cycle-dependent release of virus is not petition RIA of synchronized cells with $^{131}I$ labeled viral proteins synthesis during the cell cycle. The rate of synthesis of gag protein shows three peaks, corresponding to the $G_1,\;late\;S\;and\;late\;G_2$ phases of cell cycle. But the rate of synthesis of env protein dose not change, suggesting that in these cells the synthesis of these two gene products in controlled seperately. In Actionomycin D treated cells, the synthesis of viral proteins decreased sharply from 8 hours after treatment, and the late S and $G_2$ peaks of gag protein synthesis were disappeared. This shows the stability of viral RNA for about 6 hours in the absence of continuing viral RNA synthesis.

• Wind and Structures
• /
• v.14 no.5
• /
• pp.383-411
• /
• 2011

Roof is an integral part of building envelope. It protects occupants from environmental forces such as wind, rain, snow and others. Among those environmental forces, wind is a major factor that can cause structural roof damages. Roof due to wind actions can exhibit either flexible or rigid system responses. At present, a dynamic test procedure available is CSA A123.21-04 for the wind uplift resistance evaluation of flexible membrane-roofing systems and there is no dynamic test procedure available in North America for wind uplift resistance evaluation of rigid membrane-roofing system. In order to incorporate rigid membrane-roofing systems into the CSA A123.21-04 testing procedure, this paper presents the development of a load cycle. For this process, the present study compared the wind performance of rigid systems with the flexible systems. Analysis of the pressure time histories data using probability distribution function and power spectral density verified that these two roofs types exhibit different system responses under wind forces. Rain flow counting method was applied on the wind tunnel time histories data. Calculated wind load cycles were compared with the existing load cycle of CSA A123.21-04. With the input from the roof manufacturers and roofing associations, the developed load cycles had been generalized and extended to evaluate the ultimate wind uplift resistance capacity of rigid roofs. This new knowledge is integrated into the new edition of CSA A123.21-10 so that the standard can be used to evaluate wind uplift resistance capacity of membrane roofing systems.

• Journal of Astronomy and Space Sciences
• /
• v.28 no.4
• /
• pp.299-304
• /
• 2011

As more satellites are designed to downlink their observed image data through the X-band frequency band, it is inevitable that the occupied bandwidth of a target satellite will overlap with that of other X-band downlink satellites. For sun-synchronized low earth orbit satellites, in particular, it can be expected that two or more satellites be placed within the looking angle of a ground station antenna at the same time. Due to the overlapping in the frequency band, signals transmitted from the adjacent satellites act as interferers, leading to degraded link performance between target satellite and ground station. In this paper, link analysis was initiated by modeling the radiation pattern of ground station antenna through a validated Jet Propulsion Laboratory peak envelope model. From the relative antenna gain depending on the offset angle from center axis of maximum antenna directivity, the ratio of received interference signal level to the target signal level was calculated. As a result, it was found that the degradation increased when the offset angle was within the first point of radiation pattern. For a 7.3 m antenna, serious link degradation began at an offset angle of 0.4 degrees. From this analysis, the link performance of the coming satellite passes can be recognized, which is helpful to establish an operating procedure that will prevent the ground station from receiving corrupted image data in the event of a degraded link.

• Korean Journal of Animal Reproduction
• /
• v.27 no.1
• /
• pp.15-23
• /
• 2003

Lactadherin (formerly known as BA46), a major glycoprotein of the human milk fat globule membrane, is abundant in human breast milk and breast carcinomas and may prevent symptomatic rotavirus infections. In this study, under the control of tissue specific and hormonal inducible mouse whey acidic protein (WAP) promote., the expression pattern of lactadherin (Ltd) in lactogenic hormone-dependent mouse mammary epithelial cell line HC11 were tested. pLNWLtd construct containing 2.4 kilobases of the WAP promoter and 1.5 kilobases of human lactadherin gene was stably transfered into HC11 cells using retroviral vector system. Integration and expression level of the transgene was estimated using PCR and RT-PCR, respectively. Prominent induction of Ltd gene under the WAP promoter was accomplished in the presence of insulin, hydrocortisone and prolactin. Compared to the control (cells cultured with insulin alone), however we observed that the WAP promoter was leaky. These data indicate that luther studies are needed in finding an appropriate promoter other than WAP promoter because of its leakiness.

• Proceedings of the KSAR Conference
• /
• 2003.06a
• /
• pp.49-49
• /
• 2003

본 연구에서는 vesicular stomatitis virus G glycoprotein (VSV-G)를 envelope로 가지는 pantropic retrovirus vector system을 이용하여 재조합 human FSH 유전자가 전이된 형질전환 닭을 생산하고자 하였다. Human FSH $\alpha$ 및 $\beta$ 유전자와 CTP linker는 human pituitary gland cDNA library에서 RT-PCR 방법을 이용하여 cloning하였으며, 각각의 fragment는 FSH$\beta$-CTP-FSH$\alpha$ 순서의 단일사슬로 연결하였다. 연결된 FSH$\beta$-CTP-FSH$\alpha$는 retroviral vector 내의 $\beta$-actin promoter의 조절 하에 도입한 후, PT67 packaging cell line에 transfection하여 virus를 생산하였으며 생산된 virus는 pantropic한 virus producing cell인 GP293에 infection하여 FSH 유전자가 도입된 virus를 생산하였다. FSH 유전자의 발현을 in vitro에서 확인하기 위하여 CHO (chinese hamster ovary) 세포에 virus를 감염시킨 후, 세포의 배양액을 취하여 electrochemilumine-scence immunoassay 방법으로 정량하였다. In vitro에서 전이 후 발현이 확인된 FSH 외래유전자의 retroviral vector virus를 초원심분리로 고농축하여 stageX의 계란의 배반엽 층에 주입하였으며, 그 결과 18%의 부화율과 91%의 부화한 닭의 유전자 전이율을 확인할 수 있었다. 전이된 유전자의 확인은 FSH$\beta$와 Neo 유전자에 대한 primer를 이용한 RT-PCR의 방법을 이용하였다. In vitro에서와는 달리 in vivo에서는 FSH 유전자의 전이는 확인되었으나 발현을 확인하지는 못하였는데, 이는 적은 수의 실험군이 형질전환율에 비해 상대적이지 못하였거나, 외래 유전자인 FSH의 발현에 의한 생리적인 부작용이 유발되어 해당개체가 부화되지 못한 것으로 추정된다. 본 문제점을 해결하기 위하여 실험군의 수를 늘리고 외래 유전자에 대한 controllable expression system이 보완될 필요성이 요구되며, 이러한 점이 해결된다면 높은 유전자 전이율에 기인하여 retrovirus를 이용한 형질전환 방법은 형질전환 가금의 생산에 있어서 매우 효율적이고 주목할 만한 방법으로 사료된다.

• KIEAE Journal
• /
• v.3 no.2
• /
• pp.37-44
• /
• 2003

The aim of this study was to analyze the thermal performance through Test-Cell of TI-wall in domestic climate. This study was carried out as follows: 1) The TI-wall was studied for ability to reduce heat loss through the building envelope and analyzed to TIM properties. 2) Test models of TI-wall were designed through the investigation of previous paper and work, measured for winter and spring, and the thermal effects were analyzed. The type of the TIM used in test model is small-celled(diameter 4mm and thickness 50mm) capillary and cement brick(density $1500kg/m^3$) was used by thermal mass. 3) Test-cell of TI-wall was calibrated from measured data and the dynamic simulation program ESP-r 9.0. In these simulations, the measured climate conditions of TaeJon were used as outdoor conditions, and the simulation model of Test-cell was developed. 4) The sensitivity analysis is executed in various aspects with standard weather files and ESP-r 9.0, and then most suitable system of TI-wall are predicted. Finally, The suitable system of TI-wall was analysed according to sizes of air gap, kinds, thickness, and the surface absorption of therm wall. The result is following. In TI-wall, Concrete is better than cement brick, at that time the surface absorption is 95%, and the most efficient thickness is 250mm. As smaller of a air gap, as reducer of convection heat loss, it is efficient for heating energy. However, ensuring of a air gap at least more than 50mm is desirable for natural ventilation in Summer.

• Asian-Australasian Journal of Animal Sciences
• /
• v.14 no.2
• /
• pp.163-169
• /
• 2001

Despite the high potency of the retrovirus vector system in gene transfer, one of the main drawbacks of has been difficulty in preparing highly concentrated virus stock. Numerous efforts to boost the virus titer have ended in unsatisfactory results mainly due to fragile property of retrovirus envelope protein. In this study, to overcome this problem, we constructed our own retrovirus vector system producing vector viruses encapsulated with VSV-G (vesicular stomatitis virus G glycoprotein). Concentration process of the virus stock by ultracentrifuge did not sacrifice the virus infectivity, resulting in more than 108 to 109 CFU (colony forming unit) per ml on most of the target cell lines tested. Application of this high-titer retrovirus vector system was tested on chicken embryos. Injection of virus stock beneath the blastoderms of pre-incubated fertilized eggs resulted in chick embryos expressing E. coli LacZ gene with 100% efficiency. Therefore, our results suggest that it is possible to transfer the foreign gene into chicken embryo using our high-titer retrovirus vector.

• Journal of the Korean Society for Aeronautical & Space Sciences
• /
• v.43 no.3
• /
• pp.204-212
• /
• 2015

The infrared lock-on range of target aircraft plays a critical role in determining the aircraft survivability. In this investigation, the effects of various UAV engine nozzle configurations on the aircraft lock-on range were theoretically analyzed. A virtual subsonic aircraft was proposed first, based on the mission requirement and the engine performance analysis, and convergent-type nozzles were then designed. After determining thermal flow field and nozzle surface temperature distribution with the CFD code, an additional analysis was conducted to predict the IR signature. Also, atmospheric transmissivity for various latitude and seasons was calculated, using the LOWTRAN code. Finally, the lock-on and lethal envelopes were calculated for different nozzle configurations, assuming the sensor threshold of the given IR guided missile. It was shown that the maximum 55.3% reduction in lock-on range is possible for deformed nozzles with the high aspect ratio.