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Retrovirus Vector-Mediated Inductional Expression of the Human Lactadherin Gene in Mouse Mammary Epithelial Cells  

권모선 (대구가톨릭대학교 의과대학 생리학교실)
구본철 (대구가톨릭대학교 의과대학 생리학교실)
정병현 (건국대학교 수의과대학)
염행철 (호서대학교 생명과학전공)
박창식 (충남대학교 동물자원과학부, 형질전환복제돼지 연구센터)
김태완 (충남대학교 형질전환복제돼지 연구센터)
Publication Information
Korean Journal of Animal Reproduction / v.27, no.1, 2003 , pp. 15-23 More about this Journal
Abstract
Lactadherin (formerly known as BA46), a major glycoprotein of the human milk fat globule membrane, is abundant in human breast milk and breast carcinomas and may prevent symptomatic rotavirus infections. In this study, under the control of tissue specific and hormonal inducible mouse whey acidic protein (WAP) promote., the expression pattern of lactadherin (Ltd) in lactogenic hormone-dependent mouse mammary epithelial cell line HC11 were tested. pLNWLtd construct containing 2.4 kilobases of the WAP promoter and 1.5 kilobases of human lactadherin gene was stably transfered into HC11 cells using retroviral vector system. Integration and expression level of the transgene was estimated using PCR and RT-PCR, respectively. Prominent induction of Ltd gene under the WAP promoter was accomplished in the presence of insulin, hydrocortisone and prolactin. Compared to the control (cells cultured with insulin alone), however we observed that the WAP promoter was leaky. These data indicate that luther studies are needed in finding an appropriate promoter other than WAP promoter because of its leakiness.
Keywords
Human lactadherin gene; Whey acidic protein promoter; Lactogenic hormones; RT-PCR;
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