• The Journal of Korean Society of Virology
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• v.27 no.2
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• pp.105-113
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• 1997

The truncated $E1_{192-283}$ and $E2_{384-649}$ genes of hepatitis C virus (HCV) linked to the gene for glutathione S-transferase (GST) were constructed and their expressions were analyzed. The $GST-E1_{192-283}$ fusion gene overexpressed the fusion protein in E. coli as a soluble form, while the $GST-E1_{192-383}$ plasmid did not express expected fusion protein. The purified $GST-E1_{192-283}$ fusion protein was efficiently cleaved by thrombin. More than 90% pure, HCV $E1_{192-283}$ protein was obtained by GST-agarose chromatography. The truncated $GST-E2_{384-649}$ fusion gene expressed the fusion protein mainly as an insoluble form, whereas the $GST-E2_{384-740}$ did not express the fusion protein. The truncated $GST-E1_{182-283}$ and $GST-E2_{384-649}$ fusion proteins reacted specifically with an HCV patient serum. In addition, mice immunized with either the purified $E1_{192-283}$ or $GST-E2_{384-649}$ proteins generated specific antibodies to each antigen. The results suggested that hydrophobic carboxyl portions of the E1 and E2 proteins might affect expression levels as well as the solubility of each fusion protein in bacteria. Also, the truncated E1 protein with Tyr-192 to Ser-283 contained antigenic epitope(s) which could be specifically recognized by an HCV patient serum.

• Journal of the Institute of Electronics Engineers of Korea SD
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• v.47 no.3
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• pp.28-34
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• 2010

In this paper, the transceiver for RFID reader using 13.56MHz as a carrier frequency and meeting International Standard ISO 14443 type A, 14443 type B and 15693 is presented. The receiver is composed of envelope detector, VGA(Variable Gain Amplifier), filter, comparator to recovery the received signal. The proposed automatic reference voltage generator, positive peak detector, negative peak detector, and data slicer circuit can adjust the decision level of reference voltage over the received signal amplitudes. The transmitter is designed to drive high voltage and current to meet the 15693 specification. By using inductor loading circuit which can swing more than power supply and drive large current even under low impedance condition, it can control modulation rate from 30 percent to 5 percent, 100 perccnt and drive the output currents from 5 mA to 240 mA depending on standards. The 13.56 MHZ RFID reader is implemented in $0.18\;{\mu}m$ CM08 technology at 3.3V single supply. The chip area excluding pads is $1.5mm\;{\times}\;1.5mm$.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.8 no.2
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• pp.151-160
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• 1997

In this paper, we have carried out the DS/CDMA with a suppressed pilot channel, which is used in receiving coherently with Rake diversity and in synchronizing the chip timing, in the mobile satellite communication. Also, we have investigated the envelope variation of a shadowed Rician fading simulator, and analyzed the error performences of DS/CDMA in the mobile satellite communication. The results showed that the error performance in the Heavy shadowing environment might be degraded more than in the Rayleigh fading environment since the fading envelopes in the former environment are varied randomly compared with those in the latter environment. And the performence of DS/CDMA system could be improved about 10 dB compared with that of narrowband QPSK system. In conclusion, DS/CDMA with a suppressed pilot channel had the best performance in the case of the suppressed pilot channel to transmission power ratio $\beta$=-8 dB, the number of complex delay profiles $N_{profile}$=32, and using these values, the error performance of DS/CDMA in Light shadowing environment was identical to the ideal QPSK error performance.

• Journal of the Korean Geotechnical Society
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• v.36 no.10
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• pp.21-31
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• 2020

In this study, Hoek-Brown constants (m_i) were calculated through nonlinear regression analyses using the results of the triaxial compression tests for the basaltic intact rocks in Jeju Island. The relationships of the m_i with the uniaxial compressive strength (UCS), Brazilian tensile strength (BTS) and UCS/BTS of the Jeju basalts were investigated, respectively. In addition, a method that can be used in determining Hoek-Brown failure envelopes including the tensile and compressive failures of the Jeju basalts has been proposed. As results, the m_i values had no clear correlations with the UCS, BTS and UCS/BTS of the Jeju basalts, but there were two strong correlations between UCS and m_i/UCS, and between BTS and m_i/BTS of the Jeju basalts. In addition, it was found that the tensile strengths calculated by the Hoek-Brown failure criterion underestimate the tensile strengths of the Jeju basalts through the relationship between the m_i and UCS/BTS of the Jeju basalts. The method presented in this study is considered to be useful in determining the Hoek-Brown failure envelope for the tensile and compressive failures of the Jeju basalts.

• Journal of Korean Library and Information Science Society
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• v.35 no.3
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• pp.79-94
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• 2004

This research studied the preservation treatment of the papered literature the national treasure 745-9 $\ulcorner$Keumganggyung-samgahae$\lrcorner$, discovered in 1995 from the Four Devas of Borimsa Buddhist Temple at Jangheung County, in the viewpoint of the bibliography. The overall features of the $\ulcorner$Keumganggyung-samgahae$\lrcorner$ were of 25.3cm width, 38.9cm height 1.5cm depth, 58 pages and of high-fine yellow-brown colored reef paper. The book was ruined physically in the shape and color. The covers of the book were deeply stained by the permeated rain and some internal pages were changing into brown color by corrosion. The treatment policies were； maintaining the status quo and capable repeated treatment. And the preservation treatment was done in the sequences of 1) 1st cleaning process, 2) repairing and correcting, 3) 2nd cleaning and preservation 4) cutting, binding the book and making the envelope and wood box. The post-preservation features of $\ulcorner$Keumganggyung-samgahae$\lrcorner$ were； total 64 pages and the size being 25.8 cm width 39.4cm of lenght, and 1.9cm of depth.

• Journal of Animal Science and Technology
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• v.47 no.2
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• pp.177-186
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• 2005

Xenotransplantation may help to overcome the critical shortage of human tissues and organs for human transplantation, Swine represents an ideal source of such organs owing to their anatomical and physiological similarities to human besides their plentiful supply, However, the use of organs across the species barrier may be associated with the risk of transmission of pathogens, specially porcine endogenous retroviruses (PERVs).• Although most of these potential pathogens could be eliminated by pathogen-free breeding, PERVs are not eliminated by this treatment. PERVs are integrated into the genome of all pigs and produced by normal pig cells and infect human cells. They belong to gamma retroviruses and are of three classes viruses: A, B and C. In the present study, PCR based cloning was performed with chromosomal DNA extracted from pigs from domestic pigs in Korea. Amplified PCR fragments of about 1.5 Kb, covering the partial env gene, were cloned into pCR2.l-TOPO vectors and sequenced. A total of 91 env clones were obtained from domestic pigs, Berkshire, Duroc, Landrace and Yorkshire in Korea. Phylogenetic analysis of these genes revealed the presence of only PERV class A and B in the proportion of 58 % and 42 %, respectively. Among these, 28 clones had the correct open reading frame: 18 clones in class A and 10 clones in class B. Since both these PERV classes are polytropic and have the capacity to infect human cells, our data suggest that proviral PERVs have the potential to generate infectious viruses during or after xenotransplantation in human.

• Analytical Science and Technology
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• v.23 no.3
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• pp.304-311
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• 2010

Near Infrared Spectroscopy (NIRs) has been applied for rapid and nondestructive paper measurement by replacing the current destructive method to the property of paper. Current standard methods for the property of paper were pH, moisture, breaking length, and folding endurance, which data were compared with spectrum of FT-NIR spectrometer. Various paper products such as copy, envelope, white, newspaper, as well as old paper produced around 1960~1980 were used as the sample. The correlation ($R^2$) and standard error of prediction (SEP) results for breaking length, folding endurance, moisture and pH are $R^2$=0.914, SEP=0.508, $R^2$=0.926, SEP=0.281, $R^2$=0.941, SEP=0.931, pH $R^2$=0.949, SEP= -0.0631, respectively. This result show that NIRs can be applied to practical application for nondestructive analysis of paper records materials.

• Review of Korean Society for Internet Information
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• v.14 no.3
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• pp.78-85
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• 2013

The revelations made possible by Edward Snowden, a contractor of the US intelligence service NSA, are a sobering reminder that the Internet is not an 'anonymous' means of communication. In fact, the Internet has never been conceived with anonymity in mind. If anything, the Internet and networking technologies provide far more detailed and traceable information about where, when, with whom we communicate. The content of the communication can also be made available to third parties who obtain encryption keys or have the means of exploiting vulnerabilities (either by design or by oversight) of encryption software. Irrebuttable evidence has emerged that the US and the UK intelligence services have had an indiscriminate access to the meta-data of communications and, in some cases, the content of the communications in the name of security and protection of the public. The conventional means of judicial scrutiny of such an access turned out to be ineffectual. The most alarming attitude of the public and some politicians is "If you have nothing to hide, you need not be concerned." Where individuals have nothing to hide, intelligence services have no business in the first place to have a peek. If the public espouses the groundless assumption that State organs are benevolent "( they will have a look only to find out whether there are probable grounds to form a reasonable suspicion"), then the achievements of several hundred years of struggle to have the constitutional guarantees against invasion into privacy and liberty will quickly evaporate. This is an opportune moment to review some of the basic points about the protection of privacy and freedom of individuals. First, if one should hold a view that security can override liberty, one is most likely to lose both liberty and security. Civilized societies have developed the rule of law as the least damaging and most practicable arrangement to strike a balance between security and liberty. Whether we wish to give up the rule of law in the name of security requires a thorough scrutiny and an informed decision of the body politic. It is not a decision which can secretly be made in a closed chamber. Second, protection of privacy has always depended on human being's compliance with the rules rather than technical guarantees or robustness of technical means. It is easy to tear apart an envelope and have a look inside. It was, and still is, the normative prohibition (and our compliance) which provided us with protection of privacy. The same applies to electronic communications. With sufficient resources, surreptitiously undermining technical means of protecting privacy (such as encryption) is certainly 'possible'. But that does not mean that it is permissible. Third, although the Internet is clearly not an 'anonymous' means of communication, many users have a 'false sense of anonymity' which make them more vulnerable to prying eyes. More effort should be made to educate the general public about the technical nature of the Internet and encourage them to adopt user behaviour which is mindful of the possibilities of unwanted surveillance. Fourth, the US and the UK intelligence services have demonstrated that an international cooperation is possible and worked well in running the mechanism of massive surveillance and infiltration into data which travels globally. If that is possible, it should equally be possible to put in place a global mechanism of judicial scrutiny over a global attempt at surveillance.

• Proceedings of the Korean Vacuum Society Conference
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• 1998.02a
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• pp.24-24
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• 1998

반도체장비를 포함하는 초고진공장비의 園훌化가 급속히 그리고 절실히 요구되고 있는 것이 현실정이다. 當面해서 실현할 국산진공장비의 대상은 廣範圍하다. 즉, 각종 진공 pump ( (rotary, dry, diffusion, cryo, ion, turbo melecular pump), 진공 chamber, 진공 line, gate valve 를 위 시 한 진공 V머ve, flange, gasket, fl않d야lU, mainpulater 퉁 진공 部品이 다. 진공계 의 핵심 은 適切하고 優良한 진공재료의 선태파 사용이다. 진공장비는 사용자가 원하는 진공도를 원하 는 시간 동안 륨空度를 유지해 주어야 한다. 진공재료 선태의 기준사항은:(1) 기체의 透過성 (2) 薰했훌 (3) 혔體放出특성 - -outgassing과 degassing- (4) 機械的 량훌度 (5) 온도 의존성 (6) 化學톡성 (7) 加I성 및 鎔接 성 (8) 課電특성 (9) 磁氣특성 (10) 高速함子 및 放射線 특성 (11) 經濟성 및 調達생 둥이 다. 우량한 초고진공계재료는 풍부하게 개발되어 왔고, 또 新材料들이 개발되고 있다. 여기에서는 주로 초고진공 내지는 극고진공계의 構造材料, 機能材料, 部品材料 일반파 몇가지 신재료의 특 성에 관해서 記述한다. M Mild SteeHSAE, 1112, 1010, 1020, 1022, etc)., S Stainless SteeHAlSI, 304, 304L, 310, 316, 321, 347): 구조재료, chamber, fl하1ges A Aluminum과 Alloys (1060, 1100, 2014, 4032, 6(뻐1): 구조재료, chamber, flanges, gaskets A AI, Al 떠loy는 SS에 代替하는 역 할올 시 작하고 있다. C Copper, Copper Alloys(C11$\alpha$)0, C26800, C61400, Cl7200): 내장인자, gasket, cryopanel, tubing T Titanium, Ziriconium, Haf띠um 및 Alloys: 특히 Ti은 10n pump 용 getter material 이 외 에 U UHV,XHV용 chamber계로서 관심올 끌고 있다. N Nickel, Nickel Alloys (200, 204, 211, monel, nichrome): 부식 방지 , 전자장치 , 자기 장치 귀 금속(Ag, Au, Pt, Pd, Rh, Ir, Os, Ru): 보조부품, gasket, filament, coating, thermocouple, 접 합부위 T TiC, SiC, zrC, HfC, TaC 둥의 탄화물과, BN, TiN, AlN 동의 질화물, 붕화물이 둥장하고 었 다. 유리: Soda Lime, Borosilicate, Potash Soda Lead: View Port, Chamber envelope C Ceramics: AlZ03, BeO, MgO, zrOz, SiOz, MgOzSiOz, 3Alz032SiOz, Z$textsc{k}$hSiOz S상N4: e electrical, thermal insulators, crucibles, boats, single crystals, sepctr려 windows 저자는 최근 저자들이 발견한 Zr-Ti-Cu-Ni-Be amorphous alloys coated cham뾰r가 radiation p proof로 이용될 수 있는 사실을 점검하고 었다 .. Z.Y. Hua 들은 Cs3Sb를 새로운 photocathode 재료로 보고하고 있다.

• Journal of the Korean Wood Science and Technology
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• v.41 no.6
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• pp.515-527
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• 2013

Building energy simulations which are mainly used in Korea have evaluated the building energy performance with the different thermal conductivity of construction materials. In order to evaluate the energy consumption accurately, the difference in thermal conductivity of the wood used in stud for wooden structure was confirmed from the each simulation. In addition, the thermal transmission of building members and the thermal bridge at the conjunction of building members according to thermal conductivity from each simulation programs were researched. The thermal conductivity of pine that has the largest variation among the energy simulations was applied to the thermal properties of studs in wooden structure. The maximum error between the maximum and minimum thermal transmission of roof, wall, and floor slab was $0.023W/m^2{\cdot}K$. Plus, that thermal bridge at Rafter junction on the roof, roof-wall joint, and floor slab-wall joint was $0.025W/m{\cdot}K$. The heat transfer image for changes in temperature and the heat exchange were analyzed by HEAT2 program. The distorted temperature lines were found around the insufficient insulated connection parts. It was predicted that the temperature at the distorted parts in the analyzed image was lower than that of the other portion of the other structures.