• The Plant Pathology Journal
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• 제39권3호
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• pp.235-244
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• 2023

Acidovorax citrulli (Ac) is a phytopathogenic bacterium that causes bacterial fruit blotch (BFB) in cucurbit crops, including watermelon. However, there are no effective methods to control this disease. YggS family pyridoxal phosphate-dependent enzyme acts as a coenzyme in all transamination reactions, but its function in Ac is poorly understood. Therefore, this study uses proteomic and phenotypic analyses to characterize the functions. The Ac strain lacking the YggS family pyridoxal phosphate-dependent enzyme, AcΔyppAc(EV), virulence was wholly eradicated in geminated seed inoculation and leaf infiltration. AcΔyppAc(EV) propagation was inhibited when exposed to L-homoserine but not pyridoxine. Wild-type and mutant growth were comparable in the liquid media but not in the solid media in the minimal condition. The comparative proteomic analysis revealed that YppAc is primarily involved in cell motility and wall/membrane/envelop biogenesis. In addition, AcΔyppAc(EV) reduced biofilm formation and twitching halo production, indicating that YppAc is involved in various cellular mechanisms and possesses pleiotropic effects. Therefore, this identified protein is a potential target for developing an efficient anti-virulence reagent to control BFB.

• 대한토목학회논문집
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• 제28권1A호
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• pp.59-67
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• 2008

원전 구조물의 내진해석에서 사용하는 시간이력 함수는 적절한 하중을 보장하기 위하여 설계응답스펙트럼에 부합할 뿐만 아니라 최소 PSD함수 이상의 PSD함수를 갖도록 함으로서 각각의 진동수에서 일정 크기 이상의 에너지를 갖고 있어야 한다. 도로교 구조물에서도 구조물의 대형화와 정교화로 인한 구조물의 공공 기능의 안전성이 더욱 강조되어, 설계 목적의 PSD함수의 규정이 필요하지만 적절한 절차가 개발이 되지 않아 현실적으로 적용하기 어려운 실정이다. 본 연구에서는 다수의 인공지진을 이용하여 표준 PSD함수를 계산하는 절차를 제시하고 현재의 도로교 내진설계 스펙트럼에 부합하는 표준 PSD 함수를 제안하였다.

• Journal of Electrical Engineering and Technology
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• 제11권2호
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• pp.529-536
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• 2016

In this paper we proposed an unsupervised algorithm to estimate the reverberation time (RT) directly from the reverberant speech signal. For estimation process we use maximum likelihood estimation (MLE) which is a very well-known and state of the art method for estimation in the field of signal processing. All existing RT estimation methods are based on the decay rate distribution. The decay rate can be obtained either from the energy envelop decay curve analysis of noise source when it is switch off or from decay curve of impulse response of an enclosure. The analysis of a pre-existing method of reverberation time estimation is the foundation of the proposed method. In one of the state of the art method, the reverberation decay is modeled as a Laplacian distribution. In this paper, the proposed method models the reverberation decay as a Gamma distribution along with the unification of an effective technique for spotting free decay in reverberant speech. Maximum likelihood estimation technique is then used to estimate the RT from the free decays. The method was motivated by our observation that the RT of a reverberant signal when falls in specific range, then the decay rate of the signal follows Gamma distribution. Experiments are carried out on different reverberant speech signal to measure the accuracy of the suggested method. The experimental results reveal that the proposed method performs better and the accuracy is high in comparison to the state of the art method.

• Smart Structures and Systems
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• 제19권5호
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• pp.487-497
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• 2017

Semi-active devices use the building's own motion to produce resistive forces and are thus strictly dissipative and require little power. Devices that independently control the binary open/closed valve state can enable novel device hysteresis loops that were not previously possible. However, some device hysteresis loops cannot be obtained without active analog valve control allowing slower, controlled release of stored energy, and is presents an ongoing limitation in obtaining the full range of possibilities offered by these devices. This in silico study develops a proportional-derivative feedback control law using a validated nonlinear device model to track an ideal diamond-shaped force-displacement response profile using active analog valve control. It is validated by comparison to the ideal shape for both sinusoidal and random seismic input motions. Structural application specific spectral analysis compares the performance for the non-linear, actively controlled case to those obtained with an ideal, linear model to validate that the potential performance will be retained when considering realistic nonlinear behaviour and the designed valve control approach. Results show tracking of the device force-displacement loop to within 3-5% of the desired ideal curve. Valve delay, rather than control law design, is the primary limiting factor, and analysis indicates a ratio of valve delay to structural period must be 1/10 or smaller to ensure adequate tracking, relating valve performance to structural period and overall device performance under control. Overall, the results show that active analog feedback control of energy release in these devices can significantly increase the range of resetable, valve-controlled semi-active device performance and hysteresis loops, in turn increasing their performance envelop and application space.

• 대한조선학회논문집
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• 제61권2호
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• pp.125-134
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• 2024

The Safe Operating Envelope (SOE) combined with Short-Take-Off and Vertical Landing (STOVL) performance is an essential consideration of a light aircraft carrier for design of hull shape with excellent seakeeping performance in terms of naval air operations as well as traditional naval ship missions such as Transit and Patrol (TAP), and Replenishment at Sea (RAS) and so on. A variety of procedures are systematically combined to determine SOE considering rather complicated missions associated with operation of aircraft onboard. The evaluation of take-off and landing safety missions onboard should consider wind effect on deck and severer seakeeping indices and standards compared with conventional naval ships. In order to support take-off and landing missions, various support activities of the crews are required. So, additional evaluation is needed for indicators such as MSI(Motion sickness Index) and MII(Motion Induced Interruptions), which are quantitative indicators of work ability that appear as a result of motion response. In this study, a standard procedure is developed including the seaworthiness performance indicators, standards, and evaluation procedures that should be considered during design of STOVL aircraft carrier. Analysis results are discussed in terns of air-wake on deck as well as seakeeping indices associated with design parameter changes in view of conceptual design of a light aircraft carrier.

• Journal of Microbiology and Biotechnology
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• 제22권11호
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• pp.1580-1587
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• 2012

Japanese encephalitis virus (JEV) envelope (E) protein holds great promise for use in the development of a recombinant vaccine. Purified recombinant E (rE) protein may be useful for numerous clinical applications; however, there are limitations in using the Escherichia coli expression system for producing high-quality rE protein. Therefore, in this study, the yeast expression system was used to generate the rE protein. For protein production using the yeast system, the full-length JEV E gene was cloned into Pichia pastoris. SDS-PAGE and immunoblotting analysis demonstrated that the rE protein had a molecular mass of 58 kDa and was glycosylated. The predicted size of the mature unmodified E protein is 53 kDa, suggesting that post-translational modifications resulted in the higher molecular mass. The rE protein was purified to greater than 95% purity using combined ammonium sulfate precipitation and a SP-Sepharose Fast Flow column. This purified rE protein was evaluated for immunogenicity and protective efficacy in mice. The survival rates of mice immunized with the rE protein were significantly increased over that of Hyphantria cunea nuclear polyhedrosis virus E protein (HcE). Our results indicate that the rE protein expressed in the P. pastoris expression system holds great promise for use in the development of a subunit vaccine against JEV.

• Architectural research
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• 제22권1호
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• pp.13-21
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• 2020

The purpose of this study was to evaluate the variation in building energy predictions caused by simulation settings related to building envelop thickness. The study assessed the ceiling depth impact on skylight energy performance through OpenStudio integrated Radiance and EnergyPlus simulation programs. A ceiling as deep as 1.5 to 3m was analyzed for skylight to roof ratios from 1% to 25%. The results indicated that the building ceiling depth negatively affected the capability of skylights to significantly reduce building energy consumption. Through a parametric analysis, the study concluded that 8%, 9%, 10% and 11% skylight to roof ratio were optimal in terms of total building energy consumption for a ceiling depth of 1.5m, 2m, 2.5m and 3m, respectively. In addition, the results showed that the usually recommended 5% skylight to roof ratio was only efficient when no ceiling depth was included in the simulation model. Furthermore, the study indicated that the building energy saved by the optimal skylight of each ceiling depth decreased as the ceiling depth deepened. The highest total building energy reduction was 9%, 7%, 5% and 3% for a ceiling depth of 1.5m, 2m, 2.5m and 3m, respectively. This study induced that the solar heat gains and daylight visible transmittance by ceiling depth were crucial in the predictions of skylight energy performance and should not be neglected through building simulation simplifications as it is commonly done in most simulation programs' settings.

• 한국건축시공학회지
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• 제16권6호
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• pp.579-585
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• 2016

3차원 이미지 스캐닝 기술은 복잡한 비정형 건축물의 외피의 정확한 형상과 위치 데이터를 확보하는데 효과적이다. 3차원 이미지 스캐닝 기술의 세부 과정인 점 군집화, 메쉬표면 분리, 넙스 표면 생성, 파라메트릭 솔리드 모델을 통하여 비정형 건축물의 형상을 구축할 수 있다. 이에 본 연구는 비정형 건축물의 외장재 제작과 시공을 위한 3차원 이미지 스캐닝에 의한 역설계 프로세스와 사례 분석을 통하여 시사점을 도출한다. 본 연구의 결과는 역설계 기술을 사용한 3차원 형상 기술과 설계 요소화 방법을 다양한 건설 프로젝트에 활용할 수 있을 것으로 판단된다.

• 한국추진공학회지
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• 제10권1호
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• pp.72-86
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• 2006

램제트 추진기술에 대한 최근 개발동향과 필요 주요기술을 분석하였다. 통합 부스터의 장점을 극대화하면서 부스터의 총역적을 다양하게 조정하는 기술, 램제트 연소실의 길이는 최소화하면서 화염을 안정시키는 기술, 비행 운용범위를 다양하도록 하는 가변노즐 기술, 장시간 작동을 가능하게 하는 열차폐 기술 등이 소개/분석되었다. 여러 나라에서 다양한 램제트 추진기술을 개발해왔으며, 원초적 기술은 어느 정도 성숙되었으며, 군사적 응용과 복합사이클을 사용하는 민수용으로의 적용이 확대되고 있는 추세이다. 보다 효과적으로 다양한 발사 프랫폼에 공통으로 적용 가능한 다용도 램제트 기술 개발을 위하여 최근에 여러 유도탄(미국의 JSSAM, 러시아의 Yakhont)들이 연구되었으며, 이는 기술 신뢰도 향상 및 개발경비 절감을 높일 수 있다는 기대로 도전적 연구가 진행되고 있다.

• 한국정보통신학회논문지
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• 제11권10호
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• pp.1845-1851
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• 2007

배경 잡음과 실내 잔향음은 음성 인식 시스템 성능 저하의 주요 이유이다. 많은 알고리즘이 음성의 잔향음 제거를 위해 개발되었다. 이 논문에서는 실내 환경에서 수정된 선형 예측 잔여(Linear Prediction Residual)를 이용하여 음질 개선을 위한 잔향음 제거 방법을 제안한다. 제안된 잔향음 제거 방법은 음성에서 성문 경계의 순간에 발생한 성도(聲道)시스템의 중요한 여기에 기반한다. 본 논문에서 제안한 방법은 3개의 센서로부터 수집한 반향신호로 각 센서에서의 시간지연 정보를 사용한다. 새로운 선형 예측 잔여신호는 선형 예측 잔여의 가중치와 힐버트 변환으로 얻은, 개선된 선형 예측 잔여 조합을 사용한다. 코히런트하게 더해진 힐버트 포락선의 특징은 잡음과 반사로 인한 큰 진폭 피크를 가지는 것이다. 깨끗한 음성의 잔여는 개선된 음성을 얻는 시변전극 필터를 일으키는데 사용된다. 본 논문에서는 반향 환경에서 성능 분석을 위해 제안된 알고리즘의 시뮬레이션을 수행하였다. 제안된 알고리즘은 실내 잔향환경에서 기존의 알고리즘에 비해 반사된 음성의 품질 향상의 결과를 보였다.