The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Proteomic and Phenotypic Analyses of a Putative YggS Family Pyridoxal Phosphate-Dependent Enzyme in Acidovorax citrulli

  • Lynn Heo (Department of Plant Science and Technology, Chung-Ang University) ;
  • Yongmin Cho (Department of Plant Science and Technology, Chung-Ang University) ;
  • Junhyeok Choi (Department of Plant Science and Technology, Chung-Ang University) ;
  • Jeongwook Lee (Department of Plant Science and Technology, Chung-Ang University) ;
  • Yoobin Han (Department of Plant Science and Technology, Chung-Ang University) ;
  • Sang-Wook Han (Department of Plant Science and Technology, Chung-Ang University)
  • Received : 2023.03.29
  • Accepted : 2023.04.17
  • Published : 2023.06.01
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Abstract

Acidovorax citrulli (Ac) is a phytopathogenic bacterium that causes bacterial fruit blotch (BFB) in cucurbit crops, including watermelon. However, there are no effective methods to control this disease. YggS family pyridoxal phosphate-dependent enzyme acts as a coenzyme in all transamination reactions, but its function in Ac is poorly understood. Therefore, this study uses proteomic and phenotypic analyses to characterize the functions. The Ac strain lacking the YggS family pyridoxal phosphate-dependent enzyme, AcΔyppAc(EV), virulence was wholly eradicated in geminated seed inoculation and leaf infiltration. AcΔyppAc(EV) propagation was inhibited when exposed to L-homoserine but not pyridoxine. Wild-type and mutant growth were comparable in the liquid media but not in the solid media in the minimal condition. The comparative proteomic analysis revealed that YppAc is primarily involved in cell motility and wall/membrane/envelop biogenesis. In addition, AcΔyppAc(EV) reduced biofilm formation and twitching halo production, indicating that YppAc is involved in various cellular mechanisms and possesses pleiotropic effects. Therefore, this identified protein is a potential target for developing an efficient anti-virulence reagent to control BFB.

Keywords

Acknowledgement

We thank J. Kim for their technical help at the BT research facility center, Chung-Ang University. This work was supported by the National Research Foundation of Korea (NRF) grant funded by the Korean government (MSIT) (No. NRF-2020R1A2C1013040), Republic of Korea. This research was also supported by the Chung-Ang University Graduate Research Scholarship in 2022 (awarded to Yongmin Cho).

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